Periodontally Involved Root Surfaces Research Articles

A Scanning Electron Microscopic (SEM) Study Of The Evaluation Between The Results by Gracey Curette And Er,Cr:YSGG Laser On Periodontally Involved Root Surfaces

People with chickenpox often gain lifelong immunity after one infection. In most cases, chickenpox's natural immunity provides significant protection against reinfection. However, patients with a second reinfection have been encountered periodically. Neither population-based nor individual data are available on the frequency of reported second Varicella infections among Indians. The possibility of third-time reinfection is very rare, even in reputed literature. Here, we discuss a 68-year-old patient with four reinfections of chickenpox. Two reputed dermatologists confirmed his infection, and he recovered fully with Acyclovir and bed rest. The primary objective of this case report is to highlight the risk of chickenpox reinfections and reinfections occurring more than two times.

Effects of Citric Acid and EDTA on Periodontally Involved Root Surfaces: A SEM Study

Root debridement by scaling and root planing may not completely remove contaminated cementum particularly in more apical areas. The smear layer formed during root planing inhibits cell migration and attachment leading to impaired marginal periodontal healing. The present study was done to compare the morphological effects of root surface demineralization using citric acid and EDTA as root-conditioning agents. Thirty fragments of human dental roots previously exposed to periodontal disease were scaled and randomly divided into the following groups of treatment: Group I: Hand instrumentation and conditioning with normal saline for 5 minutes as control; group II: Hand instrumentation and conditioning with citric acid (pH 1) for 5 minutes and group III: Hand instrumentation and conditioning with EDTA (pH-7.4) for 5 minutes. Scanning electron microscopy was used to check for the presence of residual smear layer and for measuring the number and area of exposed dentin tubules. The mean efficacy of smear layer removal was compared between groups I and II, groups I and III and groups II and III, it was found to be statistically significant at 5%. When the mean diameter of the dentinal tubules and mean total surface area occupied by the dentinal tubule orifices was compared between groups II and III it was found to be statistically significant at 1% level of significance. Citric acid causes greater degree of morphological alterations than EDTA and is considered to be a better root-conditioning agent. However, the use of EDTA cannot be ruled out. This study supports the hypothesis that demineralizing agents can be used as an adjunct to the periodontal treatment aiming at restoring the biocompatibility of roots and helps in choosing an appropriate agent for root conditioning.

The Attachment of V79 and Human Periodontal Ligament Fibroblasts on Periodontally Involved Root Surfaces Following Treatment with EDTA, Citric Acid, or Tetracycline HCL: An SEM in vitro Study

Abstract Objective The present in vitro study has been designed to establish and compare the effects of citric acid, EDTA, and tetracycline HCl on human periodontally diseased roots on the structure, attachment, and orientation of V79 (primary Chinese hamster lung fibroblasts) cells and human periodontal ligament fibroblasts (HPDL). Materials and Methods Commercially availableV79 cells and HPDL derived from healthy human third molars were used in this study. These fibroblasts were left in solution for seven days in order to attain confluence. Forty single-rooted teeth were obtained from patients diagnosed with periodontitis. The crown part was removed under constant irrigation and the root was split vertically into two equal halves, thus, yielding 80 specimens. Following scaling and root planing, the specimens were washed with phosphate buffered saline (PBS) and kept in 50 μg/ml gentamycin sulphate solution for 24 hours. The root pieces were then treated as follows: citric acid at pH 1, 24% EDTA, or with a 10% solution of tetracycline HCl and were then placed in V79 fibroblast cultures and HPDL cultures. The specimens were harvested after four weeks and were fixed in 2.5% glutaraldehyde in PBS before preparation for scanning electron microscopy (SEM). Results The behavior of V79 cells was similar to that of human periodontal ligament cells on root conditioned surfaces. V79 and HPDL showed a healthy morphology on root surfaces treated with citric acid and EDTA and a relatively unhealthy appearance on root surfaces treated with tetracycline HCl and distilled water (control group). Conclusion The results suggest the use of citric acid and EDTA as root conditioning agents favorably affects the migration, attachment, and morphology of fibroblasts on human root surfaces, which may play a significant role in periodontal healing and regeneration. Citation Chandra RV, Jagetia GC, Bhat KM. The Attachment of V79 and Human Periodontal Ligament Fibroblasts on Periodontally Involved Root Surfaces Following Treatment with EDTA, Citric Acid, or Tetracycline HCL: An SEM in vitro Study. J Contemp Dent Pract 2006 February;(7)1:044-059.

The Attachment of V79 and Human Periodontal Ligament Fibroblasts on Periodontally Involved Root Surfaces Following Treatment with EDTA, Citric Acid, or Tetracycline HCL: An SEM in vitro Study

The present in vitro study has been designed to establish and compare the effects of citric acid, EDTA, and tetracycline HCl on human periodontally diseased roots on the structure, attachment, and orientation of V79 (primary Chinese hamster lung fibroblasts) cells and human periodontal ligament fibroblasts (HPDL). Commercially available V79 cells and HPDL derived from healthy human third molars were used in this study. These fibroblasts were left in solution for seven days in order to attain confluence. Forty single-rooted teeth were obtained from patients diagnosed with periodontitis. The crown part was removed under constant irrigation and the root was split vertically into two equal halves, thus, yielding 80 specimens. Following scaling and root planing, the specimens were washed with phosphate buffered saline (PBS) and kept in 50 microg/ml gentamycin sulphate solution for 24 hours. The root pieces were then treated as follows: citric acid at pH 1, 24% EDTA, or with a 10% solution of tetracycline HCl and were then placed in V79 fibroblast cultures and HPDL cultures. The specimens were harvested after four weeks and were fixed in 2.5% glutaraldehyde in PBS before preparation for scanning electron microscopy (SEM). The behavior of V79 cells was similar to that of human periodontal ligament cells on root conditioned surfaces. V79 and HPDL showed a healthy morphology on root surfaces treated with citric acid and EDTA and a relatively unhealthy appearance on root surfaces treated with tetracycline HCl and distilled water (control group). The results suggest the use of citric acid and EDTA as root conditioning agents favorably affects the migration, attachment, and morphology of fibroblasts on human root surfaces, which may play a significant role in periodontal healing and regeneration.

Er:YAG Laser in Defocused Mode for Scaling of Periodontally Involved Root Surfaces: An In Vitro Pilot Study

The Er:YAG laser may be used on periodontally involved teeth in combination with conventional periodontal therapy in order to improve the efficacy of root instrumentation. The aim of this study was to compare the effect of hand instrumentation on root surfaces of periodontally involved teeth with Er:YAG laser application. Thirty freshly extracted, non-carious, single-rooted, periodontally diseased human teeth from adult humans with advanced periodontal disease were used in this study. The teeth were divided into three groups of 10 specimens each. Group A was treated with scaling and root planing (SRP) with curets only (control). In group B, the root surfaces were scaled with curets and then lased with an Er:YAG laser (wavelength 2.94 microm). A handpiece with a water spray was used in non-contact mode (defocused) at a distance of 1 cm from root surface. Laser parameters were set at energy of 100 to 200 mJ/pulse, with 10 Hz of frequency. In group C, the root surfaces were lased only with power settings 250 to 300 mJ/pulse and 10 Hz frequency. An epon-araldite plastic embedding technique was used for light microscopic investigation. Histologic findings showed significant differences between the test and control sites. In control sites, after hand instrumentation, the surface was smooth, without a cementum layer, and the dentin layer presented opened tubules. Defects on the dentin layer were also present along root surfaces. In the test sites (B, C) root surfaces revealed no thermal damage; no cracking or tissue carbonization were observed. The superficial layers of lased surfaces appeared smooth and melted without alterations. Based on these findings, it appears that it may be feasible to use the Er:YAG laser for root instrumentation without prior root planing if the proper parameters are followed.

The periodontally-involved root surface.

Recent years have seen much research on the periodontally-involved root surface. Many of these studies have produced results which suggest that plaque contaminants of the root surface are only superficially placed, and capable of being removed by gentle means. Further research has attested to the difficulties in rendering periodontally-involved root surfaces free of calculus deposits by instrumentation, yet clinical studies show that periodontal disease can be managed by root planing. It is concluded that root surface debridement is best assessed on the basis of the healing response and that it should aim to disrupt plaque on and remove plaque from the periodontally-involved root surface rather than to remove part of the root surface itself.

歯面研磨用バーによる処置歯根面に対する培養細胞付着に関する研究 走査型電子顕微鏡による観察

歯面研磨用バーによる処置歯根面に対する培養細胞付着に関する研究 走査型電子顕微鏡による観察

Topical Application of Doxycycline on Periodontally Involved Root Surfaces in Vitro: Comparative Analysis of Substantivity on Cementum and Dentin

In this study, substantivity of topically applied doxycycline hydrochloride on root surfaces obtained from patients with periodontal disease was studied in vitro. Cementum and dentin specimens were impregnated with aqueous solutions of doxycycline for 3 minutes, and incubated in serum for 10 minutes, 7 days, and 14 days. Substantivity was determined by agar diffusion inhibition assay. Comparative analyses of cementum versus dentin groups were conducted with 2-way analysis of variance. Results indicated that there was no statistically significant differences between the binding potential of doxycycline to cementum and dentin specimens. Antibacterial effect of specimens treated with the concentration of 100 mg/ml persisted for 14 days, indicating the presence of doxycycline in biologically active form. Our findings demonstrate the long lasting substantivity of doxycycline hydrochloride on periodontally diseased root surfaces and supports the concept of using root surfaces as a substrate for the deposition and slow release for local tetracycline delivery.

New attachment in monkeys with experimental periodontitis with and without removal of the cementum

Experimental periodontitis was induced in monkeys by means of elastic orthodontic ligatures. The periodontally-involved teeth were then treated with different methods. At the end of the treatment period, the monkeys were sacrificed. The premolar-molar areas were dissected out and embedded in a low-viscosity embedding medium (Spurr). The results of the treatment procedures were analyzed on ground sections. Formation of new cementum and new bone was determined by means of tetracycline labeling. Root planing with an ultrasonic device or with hand instruments almost completely removed the cementum and a portion of the peripheral dentin. The root surface was more uneven after treatment with the ultrasonic device than after using hand instruments. No new attachment could be found after root planing. Cleaning of the periodontally-involved root surfaces with two detergents (cetylpyrimidinium chloride and sodium-N-lauroyl sarcosine) without root planing resulted in a significant amount of new attachment.

Assessment of plaque by image analysis.

The uneven distribution and pattern of staining, revealed on root-planed, periodontally-involved root surfaces by the application of a plaque-disclosing solution, precludes the use of conventional plaque scoring indices or other methods of assessment based on planimetric and gravimetric techniques. A method has been developed and evaluated to quantify stained areas using image analysis. The method is reproducible, with an error factor of 1.32%, and represents a comparatively simple, yet effective technique for quantifying plaque on irregular root surface areas.

In Vitro Cytotoxicity of Periodontally Diseased Root Surfaces

The purpose of this experiment was to develop a model system for controlled in vitro testing of root surface toxicity. Human gingival fibroblasts were placed into a depression created in the root surfaces of ten heat sterilized periodontally involved teeth, one half of which were root planed to remove all irritants. The teeth and fibroblasts were incubated in culture medium for 5 days, after which the cells were fixed and stained. Each tooth was examined for migration of cells onto the root planed and non-root planed areas of the tooth, and for evidence of any cytotoxic reaction. No difference in pattern of cell growth, cell migration, or cytotoxic reaction was discernible between root planed and non-root planed areas. Normal fibroblasts could be detected on the surfaces of dentin, calculus, periodontally involved cementum, and enamel. It appears that heat-sterilized periodontally-involved root surfaces, as well as calculus and calculus-laden enamel, were not toxic to human gingival fibroblasts in cell culture.