Direct numerical simulation (DNS) of turbulent wall-bounded flows requires long streamwise computational domains to establish the correct spatial evolution of large-scale structures with high fidelity. In contrast, experimental measurements can relatively easily capture large-scale structures but struggle to resolve the dissipative flow scales with high fidelity. One methodology to overcome the shortcomings of each approach is by incorporating experimental velocity field measurements into DNS as an inflow boundary condition. This hybrid approach combines the strengths of DNS and experimental measurements, allowing for a reduction in the streamwise computational domain and accelerated development of large-scale structures in turbulent wall-bounded flows. To this end, this paper reports the results of an investigation to establish the impact of limited spatial resolution and limited near-wall experimental inflow data on the DNS of a wall-bounded turbulent shear flow. Specifically, this study investigates the spatial extent required for the DNS of a turbulent channel flow to recover the turbulent velocity fluctuations and energy when experimental inflow data is typically unable to capture fluctuations down to the viscous sub-layer or the smallest viscous scales (i.e. the Kolmogorov scale or their surrogate viscous scale in wall-bounded turbulent shear slows) is used as the inflow to a DNS. A time-resolved numerically generated experimental field is constructed from a periodic channel flow DNS (PCH-DNS) at Reτ=\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$$Re_{\ au } =$$\\end{document} 550 and 2300, which is subsequently used as the inflow velocity field for an inflow–outflow boundary conditions DNS. The time-resolved experimental inflow field is generated by appropriately filtering the small scales from the PCH-DNS velocity by integrating over a spatial domain that is representative of a particle image velocimetry interrogation window. This study shows that the recovery of small scales requires a longer domain as the spatial resolution at the inflow decreases with all flow scales recovered and their correct scale-dependent energy is re-established once the flow has developed for 3 channel heights.