Single Cell Performance Research Articles

Enhancement effects of Ga-Gd Co-doping on the structure, conductivity, and interfacial polarization of CeO2-based electrolytes

Gd0.1Ce0.9-xGaxO2-δ (x = 0, 0.04,0.07, 0.12) are synthesized via the citric acid-EDTA combustion method as an electrolyte material for intermediate-temperature solid oxide fuel cells. XRD and Rietveld refinement revealed that the material exhibits a single-phase cubic fluorite structure. SEM and EDS analyses demonstrated that the incorporation of Ga ions improved the sample's density, increased the grain size, and provided better element dispersion. XPS analysis confirms that Ga-Gd co-doping leads to increased oxygen vacancy concentration and modifies the oxidation states of Ce. Impedance spectroscopy results indicated that Ga-Gd co-doped ceria has higher ionic conductivity and lower activation energy compared to Gd single-doped ceria. In comparison to Gd0.1Ce0.9O1.95, the best-performing Gd0.1Ce0.83Ga0.07O2-δ achieved nearly a 26-fold increase in ionic conductivity and a 66 % reduction in activation energy. The clearance coefficient of Gd0.1Ce0.83Ga0.07O2-δ reached 100.95, while the blocking factor decreased by nearly 73 % compared to Gd0.1Ce0.9O1.95. The incorporation of Ga ions led to a nearly one order of magnitude reduction in the dissipation of oxygen vacancy concentration in the space charge layer, with the potential decreasing from 0.3971V (Gd0.1Ce0.9O1.95) to 0.3117V (Gd0.1Ce0.83Ga0.07O2-δ). Dielectric studies clarified the contribution of the Gd-Ga synergistic effect on interfacial polarization. In terms of single-cell performance, the NiO-GDC| Gd0.1Ce0.83Ga0.07O2-δ|LSCF cell exhibited higher power density and current density, particularly showing a 42.9 % performance improvement at 650 °C compared to NiO-GDC|Gd0.1Ce0.9O1.95|LSCF. Density functional theory calculations indicated that the incorporation of Ga ions effectively suppresses the electronic conductivity of CeO2 and significantly reduces the formation energy, binding energy, and migration barrier of oxygen vacancies. Therefore, Ga-Gd co-doping can be regarded as an effective strategy for enhancing the performance of ceria-based electrolytes in IT-SOFCs.

Redox flow stacks with tubular cell design—Feasibility and performance

Redox flow batteries are a reliable option for the storage of energy from renewable resources and the all vanadium cell chemistry features the highest level of commercialization. The storage market demands further cost reductions. A tubular cell design can lower power specific costs by featuring reduced material needs and enabling the use of cost efficient extrusion processes of cell components. The feasibility of tubular all vanadium cells has already been shown in previous studies of the authors. In this study, we report on the progress of a recent cell generation and the integration of single cells into stacks. The performance of the current cell and stack generation has reached the region of planar cells with discharge current densities of idch> 300mAcm-2 at SOC≈0.5 and Ucell=0.8V as well as maximum power densities of P/Area> 300mWcm-2 with respect to the membrane area and of P/Volume> 750kWm-3 with respect to the cell body volume. The single cell performance turns out to be reproduceable with negligible losses at stack level in a 5p setup. In this way, the feasibility of tubular stacks is demonstrated. We claim the tubular approach to be transferrable to other cell chemistries.

Enhancing the performance of unitized regenerative proton exchange membrane fuel cells through microwave-synthesized chitosan based nanocomposites

The membrane electrode assembly (MEA) is a core component of unitized regenerative proton exchange membrane fuel cells (UR-PEMFCs). The studies aimed to improve the cell performance and reduce the cost of the MEAs for the widespread adoption of UR-PEMFCs. The present study focuses on modifications of MEA. For this purpose, an innovative nanocomposite electrocatalyst was developed by using a carbon-based support material containing platinum nanoparticles with a diameter of approximately 20–30 nm via microwave synthesis technique. The electrocatalyst was developed by a single-step process, consist of multi-walled carbon nanotubes (MWCNT), graphitic carbon nitride (g-C3N4), chitosan (Chi), and platinum nanoparticles (MWCNT/g-C3N4/Chi/Pt nanocomposite). With the development of this support material, a relatively economical and effective electrocatalyst was obtained by large surface area and using the platinum on this surface at the nano level. The prepared catalyst was applied to commercially available membrane electrode assemblies with an active area of 100 cm2. Single-cell and triple-stack performance tests were conducted, and an increase of 17.13 % in the electrolyzer mode and 16.98 % in the fuel cell mode was achieved in single-cell performance with this applied electrocatalyst. Furthermore, an enhancement of 16.96 % in the electrolyzer mode and 16.81 % in the fuel cell mode was discerned in the UR-PEMFC stack. Beside the experimental studies, a numerical model of the modified membrane properties has been developed and validated through experimental data.

In situ synthesis and deposition of palladium nanoparticles on gas diffusion layers via gamma radiolysis for cathode electrodes in proton exchange membrane fuel cells

This study investigates gamma radiolysis technique for synthesising and depositing palladium (Pd) nanoparticles on gas diffusion layers (GDLs). The Pd-coated GDLs were then used to fabricate MEAs, which were evaluated for their single-cell performance. The fabricated PEMFCs with Pd-loaded GDLs successfully generated potential and produced electricity, with the PEMFC with a Pd-loaded GDL prepared using a 0.05 M Pd precursor solution and 50 KGy dose achieved performances comparable to the PEMFC with commercial gas diffusion electrodes (GDEs). This study represents the first demonstration of a functional PEMFC using a novel gamma radiolysis-synthesised Pd catalyst GDL as the cathode electrode.

Advancements in Battery Management through Multi-Scale Digital Twin Technology: Principles and Applications

To achieve peak carbon and carbon neutral goals, the development of electric cars has become strategically important. It is necessary to have precise battery management technology because the lifespan and safety of power batteries change dynamically as they are used. This leads to rapid capacity degradation brought on by the inconsistent performance of single cells and thermal runaway brought on by short board batteries or internal defects. New battery management capabilities have been made possible by the development of the digital twin model, which is now one of the technical trends in the industry. Based on the development trend of battery management technology , the article concentrates on the analysis of basic principles of battery digital twin modeling from the aspects of system modeling to management and control requirements. The article systematically introduces the construction method of multi-dimensional, multi- scale and multi-physical field fusion of digital twin battery. Combined with the previous research of the team, the practical case of the digital twin battery was analyzed. Finally, the application perspective of digital twin battery is discussed in production design, life cycle management and other scenarios, which provided ideas and references for the development of battery management technology.

Pt-decorated oxygen-vacancy-tuned high temperature hydrogen-annealed WO3 as an efficient anode electrocatalyst for PEMFC

One of the most significant steps for the widespread application of Proton Exchange Membrane Fuel Cells (PEMFC) is the development of an efficient and corrosion-resistant electrocatalyst for Hydrogen Oxidation Reaction (HOR). To achieve an enhanced electrocatalytic activity towards HOR, we developed a carbon-free electrocatalyst that performs on par with the Pt/C commercial-based catalyst. Pt nanoparticles dispersed on hydrogenated WO3 surfaces were synthesized and experimentally shown to be an efficient HOR electrocatalyst. Furthermore, different characterizations and electrochemical studies validate the performances of all the hydrogenated samples. Pt/a-WO3/H400, hydrogenated at 400 °C, exhibits excellent electrocatalytic activity in terms of onset potential, limiting current density, and electrochemical surface area (ECSA). The membrane electrode assembly (MEA) was fabricated, and its single-cell performance was carried out using Pt/a-WO3/H300, Pt/a-WO3/H400, and Pt/a-WO3/H500 at the anode with Pt loading of 0.125 mg/cm2. The single-cell performance of Pt/a-WO3/H400 as anode electrocatalyst has the highest power density (540 mW/cm2) at 50 °C and high durability, compared to Pt/C. The enhanced electrocatalytic performance is attributed to the synergistic catalytic effect between Pt nanoparticles and the WO3-x interface. This occurs due to the preferential orientation of the highly active (002) facet and the creation of an optimum amount of oxygen vacancies due to the hydrogenation impact.

Microstructural Design of PEFC Electrocatalyst Layer Using Mesoporous Carbon

Heavy-duty applications of polymer electrolyte fuel cells (PEFCs) require improved activity and durability of the cathode electrocatalysts. The nanostructure and microstructure of the catalyst layer both significantly affect PEFC performance. Mesoporous carbons (MC) have mesopores which have been shown to suppress ionomer poisoning, when used as a catalyst support, leading to higher catalytic activity. Here, we compare the initial performance of single cells using Ketjen black (KB) and MC catalyst supports. Furthermore, the fuel cell performance is evaluated using Pt-Ta-Co alloy-based catalyst decorated on MC. Pt-Ta-Co/MC electrocatalyst exhibits high cell performance in a low current density range. However, cell performance at high current densities has yet to be improved due to non-uniform microstructure of the Pt-Ta-Co/MC electrocatalyst layer.

Microstructural Design of PEFC Electrocatalyst Layer Using Mesoporous Carbon

Heavy-duty applications of polymer electrolyte fuel cells (PEFCs) require improved activity and durability of the cathode electrocatalysts. The nanostructure and microstructure of the catalyst layer both significantly affect PEFC performance. Mesoporous carbons (MC) have mesopores which have been shown to suppress ionomer poisoning, when used as a catalyst support, leading to higher catalytic activity. Here, we compare the initial performance of single cells using Ketjen black (KB) and MC catalyst supports. Furthermore, the fuel cell performance is evaluated using Pt-Ta-Co alloy-based catalyst decorated on MC. Pt-Ta-Co/MC electrocatalyst exhibits high cell performance in a low current density range. However, cell performance at high current densities has yet to be improved due to non-uniform microstructure of the Pt-Ta-Co/MC electrocatalyst layer.

Experimental investigation for the influence mechanism of air intake method and humidity level on performance of proton exchange membrane fuel cells

As pivotal component of hydrogen fuel cell vehicles (HFCVs), proton exchange membrane fuel cells (PEMFCs) play a crucial role in determining performance and energy efficiency of HFCVs. In this study, comprehensive bench tests of fuel cell stack and electrochemical impedance spectroscopy (EIS) tests of single cells were conducted, and influence mechanisms of air intake methods and humidity levels on performance of stack and single cells at different locations were analyzed in depth. The results indicate that counter-flow inlet method exhibits superior voltage output performance and stability across various relative humidity (RH) and current densities (CDs). A moderate increase in RH can mitigate performance degradation, but excessive humidity can lead to flooding. When RH is increased to 60%, the voltage difference between co-flow and counter-flow of fuel cell stack does not exceed 0.05 V at 1.6 A/cm2. Meanwhile, the performance of cells near inlet end plate is consistently worse than those farther away under different inlet conditions. In addition, the performance trend of single cells in serpentine channels aligns with that of fuel cell stack, underscoring strong adaptability of mechanisms influencing performance under different conditions. These findings provide theoretical guidance and data support for optimizing performance and preventing failures in PEMFCs.

Study on Attenuation Mechanism and Durability Improvement of Platinum-Carbon Catalysts for Proton Exchange Membrane Fuel Cells

The relationship between Pt particle size and activity and durability of Pt/C electrocatalysts on rotating disk electrodes is established and the results show that Pt/C electrocatalysts with particle size over 3.5 nm have excellent resistance to Pt particle decay and carbon corrosion. Further, the research results on the mechanism of Pt particle decay and carbon corrosion show that the Pt particle attenuation is composed of 80% Ostwald ripening and 20% particle agglomeration, and the carbon corrosion is affected by the catalytic action of Pt particles. Therefore, the above results show that regulating the Pt particle size to 3.5–4.0 nm can improve the durability of Pt/C electrocatalysts on RDE. To verify the accuracy of this conclusion and determine the optimal particle size range in practical application, single cells with 5 × 5 cm2 is assembled to evaluate the performance and durability of cathode Pt/C electrocatalysts under H2/air. The results show that cathode Pt/C electrocatalysts with particle size between 3.5 nm and 3.8 nm have high single cell performance (2.3 A cm−2@0.65 V) and durability (A loss of 15 mV@0.8 A cm−2 after 30000 cycles). These findings reveal the attenuation mechanism of Pt/C electrocatalysts and provide ideas for the development of high-durability Pt-based electrocatalysts for practical applications.

Impact of using dual back surface field layers of different materials on GaAs single junction solar cell performance

This research aspires to investigate the impact of employing dual BSF layers on the performance of single junction GaAs solar cells using the Silvaco TCAD simulator. A layer of GaAs, InGaP, and InAlGaP has been implemented as a second BSF layer on top of the original BSF layer of the n-InGaP/n-GaAs/p-GaAs/p-InAlGaP structured solar cell. The results show that using GaAs as a second BSF layer has increased the carrier’s recombination and degraded the cell efficiency due to its lower energy bandgap, which creates a potential well that lessens the number of photogenerated carriers flowing through the conduction band toward electrodes. However, adding InGaP and InAlGaP as a second BSF layer decreases the recombination rate and generates a broad electric field region leading to extra photogenerated carriers drifting through the cell, which increases the efficiency from 29.42% to 29.81% for the case of using InGaP and 30.33% for the case of using InAlGaP. Furthermore, increasing the thickness and doping of the second BSF layer reduces the carriers’ recombination at the boundaries of this layer, which implies efficiency enhancement.

Bioadhesive-Inspired Ionomer for Membrane Electrode Assembly Interface Reinforcement in Fuel Cells.

Anion exchange membrane fuel cells promise a sustainable and ecofriendly energy conversion pathway yet suffer from insufficient performance and durability. Drawing inspiration from mussel foot adhesion proteins for the first time, we herein demonstrate catechol-modified ionomers that synergistically reinforce the membrane electrode assembly interface and triple-phase boundary inside catalyst layers. The resulting ionomers present exceptional alkaline stability with only slight ionic conductivity declines after treatment in 2 M NaOH aqueous solution at 80 °C for 2500 h. Adopting catechol-modified ionomer as both anion exchange membrane and binder achieves a single-cell performance increase of 34%, and more importantly, endows fuel cell operation at a current density of 0.4 A cm-2 for over 300 h with negligible performance degradation (with a cell voltage decay rate of 0.03 mV h-1). Combining theoretical and experimental investigations, we reveal the molecular adhesion mechanism between the catechol-modified ionomer and Pt catalyst and illuminate the effect on the catalyst layer microstructure. Of fundamental interest, this bioadhesive-inspired strategy is critical to enabling knowledge-driven ionomer design and is promising for diverse membrane electrode assembly configurational applications.

Effects of voltage stress conditions on degradation of iridium-based catalysts occurring during high-frequency operation of PEM water electrolysis

Proton exchange membrane water electrolysis (PEMWE) is needed to store renewable energy in the form of hydrogen. To improve the performance of PEMWE, elucidating the degradation mechanism of iridium (Ir)-based catalysts used for oxygen evolution reaction (OER) that is a rate-determining step is important. To date, it is known that one important reason for that is undesirable change in crystal structure of Ir. To further evaluate its degradation mechanism, in this study, effects of operating voltage conditions on crystal structure of Ir are investigated because the voltage conditions can accelerate changes in crystal structure of Ir. Especially how Ir was changed into an unfavorable metallic form was analyzed. PEMWE single cells under conditions of (i) a constant voltage of 1.9 V, (ii) a voltage cycle of 1.7–1.9 V, then a constant voltage of 1.9 V, and (iii) an applied voltage cycle with open circuit voltage (OCV). In the OCV condition, the crystalline structure of Ir was irreversibly transformed into a metallic form. This transformation induced lower OER activity than other crystalline states of Ir, followed by lower performance of PEMWE single cells. These findings help to establish baseline protocols for the stable operation of PEMWEs using Ir-based catalysts.

Construction of X-Co-tri@SSNF reinforced PPS composite proton exchange membranes and their performance in fuel cells

In this study, we pioneered the introduction of benzophenone (DPK) as a solid solvent into polyphenylene sulfide (PPS) melt-blown, which effectively reduced the PPS melt-blown processing temperature and significantly improved the coefficients of variation of PPS melt-blown nonwoven materials. X-Co-tri@SSNF proton exchange membranes with excellent performance were prepared by functionalized modification of PPS melt-blown fabric as a substrate. The structure and properties of X-Co-tri@SSNF proton exchange membranes were systematically characterized. The results demonstrate that the 1-Co-tri@SSNF PEM exhibited a high proton conductivity of 0.183 S cm−1 at 80 °C and high humidity, significantly higher than the commercial Nafion 117 (0.135 S cm−1). Due to the heat resistance of PPS itself and the continuous dense network structure substrate of PPS nonwoven fabrics as well as the high compatibility with the membrane casting liquid, the hybrid membrane interfaces were tightly connected, so it makes the hybrid membrane perform better in terms of mechanical stability (tensile strength of 36.7 MPa), methanol permeability (4.78 × 10−7 cm2 s−1), selectivity (16.32 × 104 S s cm−3), and thermal stability (thermal decomposition temperature of around 450 °C). In addition, the 1-Co-tri@SSNF membrane electrode module exhibited excellent single-cell performance with an open-circuit voltage of 629.91 V and a peak power density of 96.29 mW cm−2 (162.9 % higher than that of Nafion 117). Therefore, the composite PEM with PPS melt-blown fabric as the matrix has a more environmentally friendly process than the commercial Nafion 117 membrane, a theoretical guidance for advancing the large-scale production of low-cost PEM.

Polycarbazole-based anion exchange membranes containing flexible side-chain linked piperidine pendants for alkaline fuel cells

In recent years, advancements in anion exchange membranes (AEMs) have notably enhanced the overall performance of anion exchange membrane fuel cells (AEMFCs). However, issues like limited alkali stability and low ionic conductivity of AEMs have become apparent. In this study, focusing on molecular design, a series of polycarbazole-based AEMs with flexible side chains, named PCP-n (where ‘n’ denotes the molar ratio of the newly synthesized monomer BHC in carbazole derivatives), were developed. The integration of long flexible side chains fosters chain movement and the clustering of piperidine cations, leading to distinct hydrophilic/hydrophobic nanoscale phase separation within the PCP-n membranes. Moreover, the PCP-n membranes demonstrate effective water management capabilities, with the PCP-90 membrane displaying a 32 % water uptake and an in-plane swelling ratio below 20 % at 80 °C. The introduction of an ether-free main chain based on carbazole derivatives along with the highly stable piperidine cationic group endows the PCP-n membranes with exceptional alkali resistance. Following a 1500-h immersion in 2 M KOH at 80 °C, the PCP-90 membrane successfully retains 79.92 % of its original ionic conductivity. Given its substantial thermal stability and mechanical strength, the PCP-90 membrane underwent testing in membrane electrode assembly and single-cell performance, achieving a notable peak power density of 275.64 mW cm−2 and an open-circuit voltage of 0.98 V. Overall, the impressive comprehensive performance of the PCP-n membranes suggests a promising future for their application.

Study on the Application Performance of Polymer Electrolyte Membrane Functionalized with Triethyl Phosphite-Modified Graphene Oxide in Fuel Cells.

In this paper, we successfully synthesize phosphoric acid functionalized graphene oxide (PGO) based on acid modification of graphene oxide. The composite membrane is further prepared by adding PGO into sulfonated poly(aryl ether ketone sulfone) containing carboxyl groups matrix (C-SPAEKS). The PGO as well as the composite membranes were characterized by a series of tests. The prepared composite proton exchange membranes (PEMs) have good mechanical and electrochemical properties. Compared to the C-SPAEKS membrane, the best composite membrane has a tensile strength of 40.7 MPa while exhibiting superior proton conductivity (110.17 mS cm-1 at 80 °C). In addition, the open-circuit voltage and power density of C-SPAEKS@1% PGO are 0.918 V and 792.17 mW cm-2, respectively. Compared with C-SPAEKS (0.867 V and 166 mW cm-2), it can be seen that our work has a certain effect on the improvement of the single cell performance. The above results demonstrate that the functionalized graphene oxide has greatly improved the electrochemical performance and even the overall performance of PEMs.

Beyond benchmarking and towards predictive models of dataset-specific single-cell RNA-seq pipeline performance

BackgroundThe advent of single-cell RNA-sequencing (scRNA-seq) has driven significant computational methods development for all steps in the scRNA-seq data analysis pipeline, including filtering, normalization, and clustering. The large number of methods and their resulting parameter combinations has created a combinatorial set of possible pipelines to analyze scRNA-seq data, which leads to the obvious question: which is best? Several benchmarking studies compare methods but frequently find variable performance depending on dataset and pipeline characteristics. Alternatively, the large number of scRNA-seq datasets along with advances in supervised machine learning raise a tantalizing possibility: could the optimal pipeline be predicted for a given dataset?ResultsHere, we begin to answer this question by applying 288 scRNA-seq analysis pipelines to 86 datasets and quantifying pipeline success via a range of measures evaluating cluster purity and biological plausibility. We build supervised machine learning models to predict pipeline success given a range of dataset and pipeline characteristics. We find that prediction performance is significantly better than random and that in many cases pipelines predicted to perform well provide clustering outputs similar to expert-annotated cell type labels. We identify characteristics of datasets that correlate with strong prediction performance that could guide when such prediction models may be useful.ConclusionsSupervised machine learning models have utility for recommending analysis pipelines and therefore the potential to alleviate the burden of choosing from the near-infinite number of possibilities. Different aspects of datasets influence the predictive performance of such models which will further guide users.

Probing the Efficiency of PPMG-Based Composite Electrolytes for Applications of Proton Exchange Membrane Fuel Cell

PPMG-based composite electrolytes were fabricated via the solution method using the polyvinyl alcohol and polyvinylpyrrolidone blend reinforced with various contents of sulfonated inorganic filler. Sulfuric acid was employed as the sulfonating agent to functionalize the external surface of the inorganic filler, i.e., graphene oxide. The proton conductivities of the newly prepared proton exchange membranes (PEMs) were increased by increasing the temperature and content of sulfonated graphene oxide (SGO), i.e., ranging from 0.025 S/cm to 0.060 S/cm. The induction of the optimum level of SGO is determined to be an excellent route to enhance ionic conductivity. The single-cell performance test was conducted by sandwiching the newly prepared PEMs between an anode (0.2 mg/cm2 Pt/Ru) and a cathode (0.2 mg/cm2 Pt) to prepare membrane electrode assemblies, followed by hot pressing under a pressure of approximately 100 kg/cm2 at 60 °C for 5–10 min. The highest power densities achieved with PPMG PEMs were 14.9 and 35.60 mW/cm2 at 25 °C and 70 °C, respectively, at ambient pressure with 100% relative humidity. Results showed that the newly prepared PEMs exhibit good electrochemical performance. The results indicated that the prepared composite membrane with 6 wt% filler can be used as an alternative membrane for applications of high-performance proton exchange membrane fuel cell.

Reinforcement mechanism and the stress-strain behaviors of geocells made by non-woven geotextile

Geocell is one of the most widely employed geosynthetics for reinforcing soil beds across diverse applications, including base layer pavement and various structures such as embankments, foundations, and retaining walls. Its reinforcement mechanisms encompass both vertical and lateral cellular confinement, enhancing load distribution. Geocells are commonly made from polymer networks like high-density polyethylene (HDPE), non-woven geotextiles, and geogrids. Each type of geocell offers special advantages and effects on the reinforcement mechanism for the soil bed, dependent on material stiffness and effective opening size. This paper aims to assess both the reinforcement mechanism and stress–strain behavior of geocells (made by non-woven geotextile). To this end, three series of plate-loading tests were conducted on reinforced sand beds. The reinforcement configurations included single cells as well as multi-cells arranged in predetermined patterns. The experiments enabled us to determine bearing capacity, vertical surface displacement and axial strain of cell wall for different pocket sizes and numbers of cells. The results suggest that cells with pocket sizes matching the diameter of the loading plate exhibit superior performance. Furthermore, a comparative analysis between performance of single cells and multi-cells revealed that incorporating adjacent cells, particularly for geocells with smaller pocket sizes, significantly mitigates loading plate settlement. In addition, the results showed that the axial strain and deformation applied on non-woven geotextiles were more pronounced for smaller pocket sizes than for cells with larger pocket sizes.

Systematic evaluation of single-cell RNA-seq analyses performance based on long-read sequencing platforms

IntroductionThe rapid development of next-generation sequencing (NGS)-based single-cell RNA sequencing (scRNA-seq) allows for detecting and quantifying gene expression in a high-throughput manner, providing a powerful tool for comprehensively understanding cellular function in various biological processes. However, the NGS-based scRNA-seq only quantifies gene expression and cannot reveal the exact transcript structures (isoforms) of each gene due to the limited read length. On the other hand, the long read length of third-generation sequencing (TGS) technologies, including Oxford Nanopore Technologies (ONT) and Pacific Biosciences (PacBio), enable direct reading of intact cDNA molecules. ObjectivesBoth ONT and PacBio have been used in conjunction with scRNA-seq, but their performance in single-cell analyses has not been systematically evaluated. MethodsTo address this, we generated ONT and PacBio data from the same single-cell cDNA libraries containing different amount of cells. ResultsUsing NGS as a control, we assessed the performance of each platform in cell type identification. Additionally, the reliability in identifying novel isoforms and allele-specific gene/isoform expression by both platforms was verified, providing a systematic evaluation to design the sequencing strategies in single-cell transcriptome studies. ConclusionBeyond gene expression analysis, which the NGS-based scRNA-seq only affords, TGS-based scRNA-seq achieved gene splicing analyses, identifying novel isoforms. Attribute to higher sequencing quality of PacBio, it outperforms ONT in accuracy of novel transcripts identification and allele-specific gene/isoform expression.