Perfect Amplification Research Articles

A Review on The impact of Transistor Configuration as an amplifier

In the electronic era, the development of the transistor is unquestionably one of the most valuable contributions in the field of Electronic components. Transistor has an application to work as a switch and as an amplifier and almost in all cases. The transistor is taken as the use of an amplifier because it gives proper biasing. In this review paper, we are going to compare the Transistor as an amplifier with three configurations and select one desirable configuration with perfect amplification & that is CE configuration and taken as used in everywhere mostly.

A Review on The impact of Transistor Configuration as an amplifier

In the electronic era, the development of the transistor is unquestionably one of the most valuable contributions in the field of Electronic components. Transistor has an application to work as a switch and as an amplifier and almost in all cases. The transistor is taken as the use of an amplifier because it gives proper biasing. In this review paper, we are going to compare the Transistor as an amplifier with three configurations and select one desirable configuration with perfect amplification & that is CE configuration and taken as used in everywhere mostly.

The Rapid Identification of Anoplophora chinensis (Coleoptera: Cerambycidae) From Adult, Larval, and Frass Samples Using TaqMan Probe Assay.

A molecular diagnostic method using TaqMan probe qPCR is presented for the identification of Anoplophora chinensis (Förster) (Coleoptera: Cerambycidae) from whole body insects (adults and larvae) and frass samples stored under different conditions. The results showed a perfect amplification of DNA from all samples; the repeatability and reproducibility of the protocol were very good, with standard deviations of inter-run and intra-run variability less than or equal to 0.5. The assay allowed to discern all A. chinensis samples from those of the other non-target wood-borer species, with 100% correspondence to the homologous sequences. No amplification or cross reactions were observed with A. glabripennis (Motschulsky) (Coleoptera: Cerambycidae), which is the most related species among those tested. The protocol was validated by an internal blind panel test which showed a good correspondence between the results obtained by different operators in the same lab. The analytical sensitivity for the lab frass with the Probe qPCR, namely the lowest amount of A. chinensis DNA that can be detected (LoD), was 0.64 pg/µl with a Cq of 34.87. The use of indirect evidence for the identification of a pest is an important feature of the method, which could be crucial to detect the presence of wood-boring insects. This diagnostic tool can help prevent the introduction of A. chinensis into new environments or delimit existing outbreak areas thanks to indirect frass diagnosis.

A novel data processing method CyC* for quantitative real time polymerase chain reaction minimizes cumulative error.

Quantitative real-time polymerase chain reaction (qPCR) is routinely conducted for DNA quantitative analysis using the cycle-threshold (Ct) method, which assumes uniform/optimum template amplification. In practice, amplification efficiencies vary from cycle to cycle in a PCR reaction, and often decline as the amplification proceeds, which results in substantial errors in measurement. This study reveals the cumulative error for quantification of initial template amounts, due to the difference between the assumed perfect amplification efficiency and actual one in each amplification cycle. The novel CyC* method involves determination of both the earliest amplification cycle detectable above background (“outlier” C*) and the amplification efficiency over the cycle range from C* to the next two amplification cycles; subsequent analysis allows the calculation of initial template amount with minimal cumulative error. Simulation tests indicated that the CyC* method resulted in significantly less variation in the predicted initial DNA level represented as fluorescence intensity F0 when the outlier cycle C* was advanced to an earlier cycle. Performance comparison revealed that CyC* was better than the majority of 13 established qPCR data analysis methods in terms of bias, linearity, reproducibility, and resolution. Actual PCR test also suggested that relative expression levels of nine genes in tea leaves obtained using CyC* were much closer to the real value than those obtained with the conventional 2-ΔΔCt method. Our data indicated that increasing the input of initial template was effective in advancing emergence of the earliest amplification cycle among the tested variants. A computer program (CyC* method) was compiled to perform the data processing. This novel method can minimize cumulative error over the amplification process, and thus, can improve qPCR analysis.

Development of an effective and efficient DNA isolation method for Cinnamomum species

Different species of Cinnamomum are rich in polysaccharide’s and secondary metabolites, which hinder the process of DNA extraction. High quality DNA is the pre-requisite for any molecular biology study. In this paper we report a modified method for high quality and quantity of DNA extraction from both lyophilized and non-lyophilized leaf samples. Protocol reported differs from the CTAB procedure by addition of higher concentration of salt and activated charcoal to remove the polysaccharides and polyphenols. Wide utility of the modified protocol was proved by DNA extraction from different woody species and 4 Cinnamomum species. Therefore, this protocol has also been validated in different species of plants containing high levels of polyphenols and polysaccharides. The extracted DNA showed perfect amplification when subjected to RAPD, restriction digestion and amplification with DNA barcoding primers. The DNA extraction protocol is reproducible and can be applied for any plant molecular biology study.

Truly noiseless probabilistic amplification

Most of the schemes for "noiseless" amplification of coherent states, which have recently been attracting theoretical and experimental interest, share a common trait: the amplification is not truly noiseless, or perfect, for non-zero success probability. While this must hold true for all phase-independent amplification schemes, in this work we point out that truly noiseless amplification is indeed possible, provided that the states which we wish to amplify come from a finite set. Perfect amplification with unlimited average gain is then possible with finite success probability, for example using techniques for unambiguously distinguishing between quantum states. Such realizations require only linear optics, no single-photon sources, nor any photon counting. We also investigate the optimal success probability of perfect amplification of a symmetric set of coherent states. There are two regimes: low-amplitude amplification, where the target amplitude is below one, and general amplification. For the low-amplitude regime, analytic results for the optimal amplification success probabilities can be obtained. In this case a natural bound imposed by the ratio of success probabilities of optimal unambiguous discrimination of the source and amplified states can always be reached. We also show that for general amplification this bound cannot always be satisfied.

Nondeterministic amplifier for two-photon superpositions

We examine heralded nondeterministic noiseless amplification based on the quantum scissors device, which has been shown to increase the one-photon amplitude of a state at the expense of the vacuum-state amplitude. Here we propose using the same basic design to perform perfect amplification in a basis set of up to two photons. The device is much more efficient than several one-photon amplifiers working in tandem. When used to amplify coherent states this advantage is shown using either fidelity or in terms of probability of sucessful action, or more strikingly in a combination of the two.

Novel intron markers to study the phylogeny of closely related mammalian species.

BackgroundMultilocus phylogenies can be used to infer the species tree of a group of closely related species. In species trees, the nodes represent the actual separation between species, thus providing essential information about their evolutionary history. In addition, multilocus phylogenies can help in analyses of species delimitation, gene flow and genetic differentiation within species. However, few adequate markers are available for such studies.ResultsIn order to develop nuclear markers that can be useful in multilocus studies of mammals, we analyzed the mammalian genomes of human, chimpanzee, macaque, dog and cow. Rodents were excluded due to their unusual genomic features. Introns were extracted from the mammalian genomes because of their greater genetic variability and ease of amplification from the flanking exons. To an initial set of more than 10,000 one-to-one orthologous introns we applied several filters to select introns that belong to single-copy genes, show neutral evolutionary rates and have an adequate length for their amplification. This analysis led to a final list of 224 intron markers randomly distributed along the genome. To experimentally test their validity, we amplified twelve of these introns in a panel of six mammalian species. The result was that seven of these introns gave rise to a PCR band of the expected size in all species. In addition, we sequenced these bands and analyzed the accumulation of substitutions in these introns in five pairs of closely related species. The results showed that the estimated genetic distances in the five species pairs was quite variable among introns and that this divergence cannot be directly predicted from the overall intron divergence in mammals.ConclusionsWe have designed a new set of 224 nuclear introns with optimal features for the phylogeny of closely related mammalian species. A large proportion of the introns tested experimentally showed a perfect amplification and enough variability in most species, indicating that this marker set can be very helpful in multilocus phylogenetics of mammals. Due to the lower variability and stronger stochasticity of nuclear markers with respect to mitochondrial genes, studies should be designed to make use of several markers like the ones designed here.

Unifying Quantum State Transfer and State Amplification

We present a Hamiltonian that can be used for amplifying the signal from a quantum state, enabling the measurement of a macroscopic observable to determine the state of a single spin. We prove a general mapping between this Hamiltonian and an exchange Hamiltonian for arbitrary coupling strengths and local magnetic fields. This facilitates the use of existing schemes for perfect state transfer to give perfect amplification. We further prove a link between the evolution of this fixed Hamiltonian and classical cellular automata, thereby unifying previous approaches to this amplification task. Finally, we show how to use the new Hamiltonian for perfect state transfer in the scenario where total spin is not conserved during the evolution, and demonstrate that this yields a significantly different response in the presence of decoherence.