Perennial Tree Research Articles

Conservation Implications of Population Structure and Dynamics in Medicinal Arbor Albizia odoratissima on Hainan Island, China

Albizia odoratissima Benth is a perennial evergreen tree valued for its medicinal properties and is indigenous to the mountainous regions of southwestern China. The population status of A. odoratissima has been sparsely studied. This study systematically evaluated the population structure and dynamics of A. odoratissima in the central mountainous region of Hainan Island, China, with the objective of informing the development of sustainable conservation strategies for the ecological restoration of its natural populations. Using the methodologies of population ecology, including the development of static life tables, population survival curves, population dynamics analysis, and time-series predictions, the results indicated that the populations of A. odoratissima on Hainan Island were geographically isolated into three groups. The age class distribution revealed that young, middle-aged, and mature individuals accounted for 5.73%, 74.94%, and 19.33%, respectively, suggesting a declining trend in the population. Moreover, the A. odoratissima population on Hainan Island was highly sensitive to anthropogenic disturbances, with significant increases in mortality rates observed at both the juvenile and mature stages. These results were likely due to the intraspecific and interspecific competitions, as well as external factors including human-induced disturbances, climate variability, and extreme weather events, which might potentially lead to the species’ future endangerment. Based on the current status of the A. odoratissima population, we develop adaptive management and forbid anthropogenic deforestation, conserving in situ and expanding populations, protecting ex situ germplasm resources, and replanting artificially, in order to manage the long-term conservation and management of A. odoratissima.

Phenolic Characterization and Quality Evaluation of Herbal Coffee from Roasted Juniper Berry Fruits (Juniperus drupacea L.): Elucidating the Impact of Roasting.

Consumers' demand for foods with health benefits and different tastes is on an increasing trend. Juniper berries ("andiz" in Turkish) are the fruits of perennial, aromatic, and resinous Juniperus drupacea trees. In this study, quality properties of herbal coffee samples obtained from juniper berries roasted at three different temperatures (120, 160, and 200 °C) and four different durations (10, 25, 32.5, and 55 min) were elucidated. The herbal coffee samples were prepared from roasted and powdered fruits, and their total phenolic contents (TPCs), sugar profiles, antioxidant activities (AAs), and other quality parameters were examined. The highest AA value was determined as 17.99 and 29.36 mM Trolox/L (DPPH and ABTS, respectively) in the herbal coffee prepared from berries roasted at 120 °C for 25 min. Sucrose and glucose were dominant in all herbal coffee samples. Sixteen phenolic compounds were identified and quantified by a LC-ESI-MS/MS device. The TPC values of the herbal coffee varied from 236.7 to 917.0 g/L, and the procyanidin dimer, amentoflavone, methyl-biflavone, and digalloylquinic acid were dominant in all samples. The hydroxymethylfurfural (HMF) content of the herbal coffee varied between 0.01 and 0.39 mg/kg. According to a sensory analysis, the herbal coffee obtained from fruits roasted at 120 °C for 25 min was the most appreciated sample. In sum, this work shows that herbal coffee is non-caffeinated and is an alternative to regular coffee drinks derived from juniper berries roasted at lower temperatures and has more significant phenolic and antioxidant contents. It also has the potential to offer innovative and healthy alternatives to the food industry. Future research should focus on investigating how this herbal coffee can be positioned in the market and can influence consumer preference.

Microbiota dynamics and source tracing during the growing, aging, and decomposing processes of Eucommia ulmoides leaves.

Eucommia ulmoides, an important tree, faces serious threat to its growth from environmental stress, particularly climate change. Using plant microbes to enhance host adaptation to respond climate change challenges has been recognized as a viable and sustainable strategy. However, it is still unclear how the perennial tree microbiota varies across phenological stages and the links between respective changes in aboveground and belowground niches. Here, we sequenced 27 root and 27 leaf samples of E. ulmoides using 16S rRNA and ITS amplicon sequencing techniques. These samples were obtained from the three main phenological stages of leaves, including leaf growing, aging and decomposing stages. Results showed that the diversity, composition, and function of the leaf microbiota of E. ulmoides showed more obvious changes at three phenological time points compared to roots. Regarding alpha diversity, the root microbiota showed no difference across three sampling stages, while the leaf microbiota varied with sampling stages. Regarding beta diversity, the root microbiota clustered from different sampling stages, while the leaf microbiota exhibited distinct separation. Regarding composition and function, the dominant taxa and main functions of the root microbiota were the same in three sampling stages, while the leaf microbiota in the decomposing stage was obviously different from the remaining two stages. Additionally, taxa overlap and source-sink relationship existed between E. ulmoides microbiota. Specifically, the degree of overlap among root microbiota was higher than that of leaf microbiota in three sampling stages. The bidirectional source-sink relationship that existed between the root and leaf niches varied with sampling stage. During the leaf growing and aging stages, the proportion of microbial members migrating from roots to leaves was higher than the proportion of members migrating from leaves to roots. During the leaf decomposing stage, the migration characteristics of the fungal community between the root and leaf niches maintained the same as in the remaining two stages, but the proportion of bacterial members migrating from leaves to roots was significantly higher than that of members migrating from roots to leaves. Our findings provide crucial foundational information for utilizing E. ulmoides microbiota to benefit their host under climate change challenges.

Changing climate risks for high-value tree fruit production across the United States

Abstract Climate change poses growing risks to global agriculture including perennial tree fruit such as apples that hold important nutritional, cultural, and economic value. This study quantifies historical trends in climate metrics affecting apple growth, production, and quality, which remain understudied. Utilizing the high-resolution gridMET dataset, we analyzed trends (1979-2022) in several key metrics across the U.S. – cold degree days, chill portions, last day of spring frost, growing degree days (GDD), extreme heat days (daily maximum temperature >34°C), and warm nights (daily minimum temperatures >15°C). We found significant trends across large parts of the U.S. in all metrics, with the spatial patterns consistent with pronounced warming across the western states in summer and winter. Yakima County, WA, Kent County, MI, Wayne County, NY—leading apple-producers—showed significant decreasing trends in cold degree days and increasing trends in GDD and warm fall nights. Yakima county, with over 48,870 acres of apple orchards, showed significant changes in five of the six metrics - earlier last day of spring frost, fewer cold degree days, increasing GDD over the overall growth period, and more extreme heat days and warm nights. These trends could negatively affect apple production by reducing the dormancy period, altering bloom timing, increasing sunburn risk, and diminishing apple appearance and quality. Large parts of the U.S. experience detrimental trends in multiple metrics simultaneously that indicate the potential for compounding negative impacts on the production and quality of apples and other tree fruit, emphasizing the need for developing and adopting adaptation strategies.

A molecular module connects abscisic acid with auxin signals to facilitate seasonal wood formation in Populus.

Perennial trees have a recurring annual cycle of wood formation in response to environmental fluctuations. However, the precise molecular mechanisms that regulate the seasonal formation of wood remain poorly understood. Our prior study indicates that VCM1 and VCM2 play a vital role in regulating the activity of the vascular cambium by controlling the auxin homoeostasis of the cambium zone in Populus. This study indicates that abscisic acid (ABA) affects the expression of VCM1 and VCM2, which display seasonal fluctuations in relation to photoperiod changes. ABA-responsive transcription factors AREB4 and AREB13, which are predominantly expressed in stem secondary vascular tissue, bind to VCM1 and VCM2 promoters to induce their expression. Seasonal changes in the photoperiod affect the ABA amount, which is linked to auxin-regulated cambium activity via the functions of VCM1 and VCM2. Thus, the study reveals that AREB4/AREB13-VCM1/VCM2-PIN5b acts as a molecular module connecting ABA and auxin signals to control vascular cambium activity in seasonal wood formation.

Soil quality and vegetation identification in closed sand-gravel mining area in West Java, Indonesia

This paper examines the soil quality and vegetation identification in a closed sand gravel mining area in West Java, Indonesia. Revegetation of disturbed mining areas is crucial in restoring soil quality and environmental function. The native plants play a pivotal role in accelerating this process. The goal of this mixed-methods study in closed sand-gravel mining was to look at the nutrient profile, texture, and microbial count of the soil in two places in the foothills of a closed mine, as well as to find and name the plant species that naturally grow well and to find and name the plant species that naturally grow well when it rains. The observation showed that the soil near the spring was more fertile than the soil away from the spring, and the soil surrounding the spring supported a higher plant density. The study area supported the growth of 22 plants, including two trees, 13 shrubs, two sedges, four grasses, and one fern. Notably, two perennial trees–Mexican lilac and beech wood–were successfully grown during the initial greening program conducted three years ago. The bacterial and fungal counts for both soils were 109 colony-forming units (CFU) per g and 104 CFU/g, respectively. We found different morphology in the soil fungal colonies, while the bacteria colonies remained homogenous. The results underscore that the soil in the disturbed mine area still supported the native plant growth. These findings are crucial for the preservation of these native plants, making them crucial for the intensive revegetation of the mining area prior to the introduction of suitable perennial trees.

Subtropical region tea tree LAI estimation integrating vegetation indices and texture features derived from UAV multispectral images

Monitoring perennial tea tree leaf area index (LAI) provides essential insights into crop growth, and unmanned aerial vehicle (UAV) -based LAI estimation has recently proven effective across various crops. In subtropical regions, the conventional farming method (CFM), involves intensive irrigation and fertilization, and the agroecological farming method (AFM) emphasizes environmentally sustainable practices. This research employs machine learning (ML) to analyze thirty-four vegetation indices (VIs) and eight texture features (TFs) derived from UAV imagery, alongside in situ LAI measurements, to estimate tea tree LAI under CFM and AFM. Additionally, minimum redundancy-maximum relevance analysis was utilized to assess the mutual information of features for regression analysis using Polynomial Regression (PR), Ridge Regression, Decision Tree Regression, and Random Forest Regression (RFR) models. The results showed that: (1) AFM had higher in situ LAI values (mean=4.323, SD=1.594) compared to CFM (mean=3.901, SD=1.816), with less seasonal variation, mainly attributed to agronomic practices like harvesting and winter pruning. (2) Optimal image features for LAI estimation were identified by extracting pixel-based features from tea tree regions to enhance the correlation between LAI and imagery. (3) Combining VIs and TFs improved LAI estimation accuracy. The RFR model, using two VIs and one TF, achieved R²=0.710 and RMSE=1.357 for CFM, while the PR model, incorporating nine VIs and 10 TFs, had R²=0.540 and RMSE=1.283 for AFM. This study demonstrates that ML techniques are promising for estimating tea tree LAI using UAV-based multispectral imagery, offering practical guidelines for efficient field management.

Pan-transcriptome analysis provide insights into resistance and fruit quality breeding of pear (Pyrus pyrifolia)

Pyrus pyrifolia, commonly known as sand pear, is a key economic fruit tree in temperate regions and possesses highly diverse germplasm resources for pear quality improvement. However, research on the relationship between resistance and fruit quality traits during the breeding of fruit species like pear is limited. Pan-transcriptomes effectively capture genetic information from coding regions and reflect variations in gene expression between individuals. Here, we constructed a pan-transcriptome composed of 506 samples from different tissues of sand pear, and explored the intrinsic relationships among phenotypes and the selection for disease resistance during improvement based on expression presence/absence variation (ePAVs). The pan-transcriptome contains 156,744 transcripts, among which the novel transcripts show significant enrichment in the defense response. Interestingly, disease resistance genes are highly expressed in landraces of pear but have been selected against during the improvement of this perennial tree species. We found that genetically diverse landraces can be divided into two subgroups and inferred that they have undergone different dispersal processes. Through co-expression network analysis, we confirmed that the formation of stone cells in pears, the deposition of fruit anthocyanins, and the ability to resist stress are interrelated. They are jointly regulated by several modules, and the expression of regulatory genes has a significant correlation. Moreover, we identified candidate genes such as HKL1 that may affect sugar content and are missing from the reference genome. Our study provides insights into the associations between complex fruit traits, while providing a database resource for pear disease resistance and fruit quality breeding.

Urban Cemeteries as Biodiversity Refuges: A Comparative Study of Plant Ecobiomorphs in Central Kazakhstan

Cemeteries are often overlooked in ecological studies, yet they represent unique urban microhabitats that contribute to the preservation of diverse plant species, including those adapted to various ecological niches. This study aimed to assess the species composition, ecological classifications, and abundance of vascular plants in the cemetery and surrounding areas to explore cemeteries’ role in conserving plant ecobiomorph diversity in arid climates. This study identified 79 plant species from 23 families within the cemetery compared with 31 species from 11 families in the surrounding area. The plant community in the cemetery was dominated by mesophytes, suggesting favorable and stable conditions for plant growth, while xerophytes were more common in the surrounding areas, indicating harsher, drier conditions. The diversity of plant life forms, including perennial herbs, shrubs, and trees, was significantly higher within the cemetery, indicating a more complex and resilient ecosystem. Our study demonstrates that cemeteries act as vital refuges for plant biodiversity. They offer significantly higher species diversity and more complex ecosystem structures compared with the surrounding areas. These findings emphasize the critical role cemeteries play in urban biodiversity conservation, particularly in increasingly arid environments.

Exploring the inhibitory activity and mechanism on lipid production in 3T3-L1 cells by hot water extract derived from Acacia confusa flowers.

Acacia confusa Merr. (Fabaceae) (A. confusa) is a native tree species of Taiwan, commonly found in the low-altitude mountains and hilly areas of the Hengchun Peninsula. This evergreen, perennial, and large-sized tree was the focus of a study that employed various chromatographic and spectroscopic methods to analyze the hot water extract of its flowers. The analysis revealed that the major components of the extract were myricitrin, quercitrin, europetin-3-O-rhamnoside, and chalconaringenin-2'-xyloside, with respective concentrations of approximately 0.22, 0.02, 0.26, and 0.10mg/g of the flowers. Subsequent cell assays were conducted to assess the inhibitory effect of the extract on lipid synthesis in fat cells. Oil Red O staining results indicated that the extract significantly suppressed fatty acid accumulation in 3T3-L1 cells, with the most pronounced effect observed at a concentration of 180μg/ml. Furthermore, the hot water extract of A. confusa flowers was found to increase the phosphorylation of AMP-activated protein kinase (AMPK), decrease the phosphorylation of cAMP response element-binding protein (CREB), and reduce the expression of glucocorticoid receptor (GR) protein. This, in turn, inhibited the expression of downstream transcription factors such as CCAT/ehancer binding proteins α (C/EBPα), CCAT/ehancer binding proteins β (C/EBPβ), CCAT/ehancer binding proteins δ (C/EBPδ), peroxisome proliferation-actived receptor γ (PPARγ), and sterol regulatory element binding proteins-1 (SREBP-1). Consequently, the expression of lipid synthesis-related proteins acetyl-CoA carboxylase (ACC), fatty acid synthase (FAS), and fatty acid translocase (CD36) was reduced, ultimately inhibiting lipid generation. Therefore, the hot water extract of A. confusa flowers shows potential for development as a weight-loss tea.

The Grapevine MADS-Box Protein VvAGL11 Induces Early Flowering in Arabidopsis

Grapevine (Vitis vinifera) is an important and popular perennial fruit tree cultivated worldwide. Grapevine ripening is affected by flowering time, and although members of the MADS-box protein family play vital roles in regulating flowering in plants, the functions of MADS-box proteins in grapevine remain largely unknown. AGAMOUS-LIKE 11 (VvAGL11), a MADS-box gene in grapevine, was reported to be a regulator of seed morphogenesis. In this study, heterologous overexpression of VvAGL11 was found to significantly promote flowering in Arabidopsis, suggesting that its active expression in grapevine may induce early flowering and ripening. Transcriptome analysis showed that VvAGL11 overexpression affected the expression of genes involved in stress responses, hormonal signaling responses, and flowering regulation. Notably, VvAGL11 significantly increased the expression of key flowering genes such as FLOWERING LOCUS T (FT), APETALA3 (AP3), and SQUAMOSA PROMOTER BINDING PROTEIN-LIKE 5 (SPL5), which might have contributed to the early flowering in Arabidopsis. In summary, we characterized a novel flowering regulator, VvAGL11, which could be a potential target for early ripening breeding in grapevine.

Preparation of Lyocell Fibers from Solutions of Miscanthus Cellulose.

Both annual (cotton, flax, hemp, etc.) and perennial (trees and grasses) plants can serve as a source of cellulose for fiber production. In recent years, the perennial herbaceous plant miscanthus has attracted particular interest as a popular industrial plant with enormous potential. This industrial crop, which contains up to 57% cellulose, serves as a raw material in the chemical and biotechnology sectors. This study proposes for the first time the utilization of miscanthus, namely Miscanthus Giganteus "KAMIS", to generate spinning solutions in N-methylmorpholine-N-oxide. Miscanthus cellulose's properties were identified using standard methods for determining the constituent composition, including also IR and atomic emission spectroscopy. The dry-jet wet method was used to make fibers from cellulose solutions with an appropriate viscosity/elasticity ratio. The structural characteristics of the fibers were studied using IR and scanning electron microscopy, as well as via X-ray structural analysis. The mechanical and thermal properties of the novel type of hydrated cellulose fibers demonstrated the possibility of producing high-quality fibers from miscanthus.

Salinity-Induced Photorespiration in Populus Vascular Tissues Facilitate Nitrogen Reallocation.

Adaptation to abiotic stress is critical for the survival of perennial tree species. Salinity affects plant growth and productivity by interfering with major biosynthetic processes. Detrimental effects of salinity may vary between different plant tissues and cell types. However, spatial molecular mechanisms controlling plant responses to salinity stress are not yet thoroughly understood in perennial trees. We used laser capture microdissection in clones of Populus tremula x alba to isolate palisade and vascular cells of intermediary leaf from plants exposed to 150 mM NaCl for 10 days, followed by a recovery period. Cell-specific changes in proteins and metabolites were determined. Salinity induced a vascular-specific accumulation of proteins associated with photorespiration, and the accumulation of serine, 3-phosphoglycerate and NH4 + suggesting changes in N metabolism. Accumulation of the GLUTAMINE SYNTHETASE 2 protein, and increased GS1.1 gene expression, indicated that NH4 + produced in photorespiration was assimilated to glutamine, the main amino acid translocated in Populus trees. Further analysis of total soluble proteins in stems and roots showed the accumulation of bark storage proteins induced by the salinity treatments. Collectively, our results suggest that the salt-induced photorespiration in vascular cells mediates N-reallocation in Populus, an essential process for the adaptation of trees to adverse conditions.

Comprehensive review on ethnomedicinal, phytochemistry and pharmacological profile of.

Ficus religiosa L. (F. religiosa) or sacred fig is a large perennial tree belonging to the family Moraceae or mulberry family. Though the tree has pan-tropical distribution but originally it is indigenous to the Indian subcontinent and Indochina region. Popularly the tree is named "Pepal or bodhi tree". Traditionally, it is practiced for the treatment of asthma, nose bleeding, heart disorders, diabetes, wound healing, ear problems, constipation, hyperlipidemia, gonorrhea, ulcers and infectious disorders. Chemical analysis demonstrated the presence of numerous bioactives including tannins, phenols, saponins, sugars, alkaloids, methionine, terpenoids, flavonoids, glycosides, proteins, separated amino acids, essential and volatile oils and steroids etc., which are probably responsible for its diverse pharmacological actions. The present work is an attempt to compile up-to-date comprehensive information on F. religiosa that covers its taxonomy, ethnomedicinal importance, phytochemistry, pharmacological attributes and clinical trials. Keeping in mind the various health attributes of F. religiosa, future research can be aimed at in-depth elucidation of the structure-function relationship and multifactorial signalings pathways.

Targeted Metabolites and Transcriptome Analysis Uncover the Putative Role of Auxin in Floral Sex Determination in Litchi chinensis Sonn.

Litchi exhibits a large number of flowers, many flowering batches, and an inconsistent ratio of male and female flowers, frequently leading to a low fruit-setting rate. Floral sexual differentiation is a crucial phase in perennial trees to ensure optimal fruit production. However, the mechanism behind floral differentiation remains unclear. The objective of the study was to identify the role of auxin in floral differentiation at the transcriptional level. The results showed that the ratio of female flowers treated with naphthalene acetic acid (NAA) was significantly lower than that of the control stage (M0/F0). The levels of endogenous auxin and auxin metabolites were measured in male and female flowers at different stages of development. It was found that the levels of IAA, IAA-Glu, IAA-Asp, and IAA-Ala were significantly higher in male flowers compared to female flowers. Next-generation sequencing and modeling were employed to perform an in-depth transcriptome analysis on all flower buds in litchi 'Feizixiao' cultivars (Litchi chinensis Sonn.). Plant hormones were found to exert a significant impact on the litchi flowering process and flower proliferation. Specifically, a majority of differentially expressed genes (DEGs) related to the auxin pathway were noticeably increased during male flower bud differentiation. The current findings will enhance our comprehension of the process and control mechanism of litchi floral sexual differentiation. It also offers a theoretical foundation for implementing strategies to regulate flowering and enhance fruit production in litchi cultivation.

Comparing Apples and Oranges: Advances in Disease Resistance Breeding of Woody Perennial Fruit Crops.

Apple and citrus are perennial tree fruit crops that are vital for nutritional security and agricultural economy and to achieve the Sustainable Development Goals of the United Nations. Apple scab and fire blight, along with Huanglongbing, canker, and tristeza virus, stand out as their most notorious diseases and annually destabilize fruit supply. An environmentally sound approach to managing these diseases is improving tree resistance through breeding and biotechnology. Perennial fruit tree germplasm collections are distributed globally and offer untapped potential as sources of resistance. However, long juvenility, specific pollination and flowering habits, and extensive outcrossing hinder apple and citrus breeding. Advances in breeding approaches include trans- and cis-genesis, genome editing, and rapid-cycle breeding, which, in addition to conventional crossbreeding, can all facilitate accelerated integration of resistance into elite germplasm. In addition, the global pool of available sources of resistance can be characterized by the existing genetic mapping and gene expression studies for accurate discovery of associated loci, genes, and markers to efficiently include these sources in breeding efforts. We discuss and propose a multitude of approaches to overcome the challenges of breeding for resistance in woody perennials and outline a technical path to reduce the time required for the ultimate deployment of disease-resistant cultivars.

Tactics for Creating Tree Bank for Community Environment and Household Economy in Thailand

This article aimed to present strategies for creating a tree bank. Activities in tree bank plots for sustainable community environmental management and raising the household economy according to the new economic model were studied. The research area is a community that has successfully managed a tree bank and has been praised as a model in 3 regions, including 3 locations: Ban Tham Suea tree bank in Phetchaburi province, Khao Chakan agroforestry tree bank in Sa Kaeo province and Ban Khlong Ruea tree bank in Chumphon province. Data were collected through in-depth interviews. Group interviews were conducted and tree survey plots in the field were prepared. Committee members of the Tree Bank and representatives of the 3 Bank for Agriculture and Agricultural Cooperatives (BAAC), 11 people each, totaling 33 people were purposive sampling. Content analysis and the abundance of plant species were analyzed using the Menhinick (1964). The research results showed that the strategy for creating a tree bank involved group forming of household members with their own land by planting trees in agroforestry plots. The group acquire wood as an asset and use trees as collateral to guarantee loans from financial institutions that are guaranteed by the state. Environmental activities were organized by planting trees of at least 25 perennial trees per rai. 58 plant species as specified by the BAAC were chosen. Nourish the soil with compost made from scraps of leaves and branches. The richness index (R2) analysis showed that the abundance of tree species in all plots was at a high level (R2 =2.5-4.5). In terms of upgrading the household economy, knowledge of the production of firewood and burn charcoal, wood vinegar from trees, wood processing for house construction and furniture products was disseminated by the tree bank by organizing learning center and eco-tourism. Collaborative networks with organizations and educational institutions were built in order to use the tree bank for sustainable development.

VvLBD39, a grape LBD transcription factor, regulates plant response to salt and drought stress

Grape (Vitis vinifera L.), as an important deciduous perennial fruit tree, constantly confronts various abiotic stresses such as salinity and drought. The lateral organ boundaries domain (LBD) proteins are a class of plant-specific transcription factors that play pivotal roles in regulating plant growth and responding to abiotic stress. However, the biological function of the LBD transcription factor in grape remains poorly understood. Here, we cloned and characterized the VvLBD39 gene from grape, which contained a highly conserved LBD domain and localized to the cell nucleus. qRT-PCR analyses showed that the expression of VvLBD39 was downregulated upon exposure to NaCl, polyethylene glycol 6000 (PEG6000) and abscisic acid (ABA) treatments, respectively. Overexpression of VvLBD39 in grape calli and Arabidopsis resulted in hypersensitivity to PEG6000 and NaCl stress. Moreover, VvLBD39-overexpressing transgenic tobacco exhibited decreased tolerance to drought and salt stress, as well as insensitivity to exogenous ABA. After drought and salt stress treatments, the chlorophyll content, root length and antioxidant enzyme activity of the transgenic tobacco were lower than those of the wild-type (WT). Conversely, malonic dialdehyde (MDA) content, electronic conductivity, hydrogen peroxide (H2O2) content and superoxide anion (O2-) productivity were markedly elevated in the transgenic tobacco compared to the WT. Further investigations found that VvLBD39 had a negative impact on stomatal closure, ABA biosynthesis and ABA signaling under drought and salt treatments. In addition, the expression of genes related to reactive oxygen species (ROS) scavenging and stress response were significantly downregulated in VvLBD39 transgenic tobacco. Taken together, these results indicated that VvLBD39 functions as a negative regulator of salt and drought tolerance, making it a promising target for drought and salt resistance breeding.

Extraction of Oil from Azadirachta indica and Moringa stenopetala Seeds and Evaluation of its Physicochemical Properties

Azadirachta indica and Moringa stenopetala trees have been regarded as underutilized, tropical plants, fast-growing, drought-tolerant, robust, oleaginous, and evergreen perennial trees growing widely in various regions of Ethiopia. Almost every part of these plants (i.e., roots, stems, foliage, seeds, and barks) can be used as food additives and as raw materials for pharmaceuticals, cosmetics, soap, and biofuel processing industries. This study aimed at the extraction and characterization of oil from A. indica and M. stenopetala seeds using the solvent method. The Box-Behnken Design was employed in the experimental design and result analysis. The particle size (0.2, 0.5, 0.8 mm), solvent-to-solute ratio (3:1, 6:1, 9:1), and extraction time (2, 5, 8 hrs) were experimental variables with three levels of low, medium, and high, whereas, the extraction temperature was kept uniform. Seventeen experiments were conducted for each species thereby developing the quadratic models with a P-value < 0.0001(significant). The quality and adequacy of the models were evaluated by analysis of variance (ANOVA) at 5% least significant difference. Results of the physicochemical determination of oils were triplicated and obtained as mean ± standard deviation. The determined physicochemical properties of A. indica and M. stenopetala seed oils were kinematic viscosity, specific gravity, pH value, refractive index, acid value, free fatty acid, saponification value, iodine value, and peroxide value. The obtained experimental results showed that the extracted oils from A. indica and M. stenopetala seeds exhibit good oil quality, and hence, they can be employed for commercial and industrial purposes, and the generation of renewable energy (biofuel).

A Perennial Green Revolution to address 21st‐century food insecurity and malnutrition

AbstractFarming practices of the past century have dramatically increased annual crop yields to unprecedented levels but have consequentially created increasing ecological and public health concerns, posing a long‐term threat to global food security. Soil tillage and chemical inputs perpetuate soil erosion, biodiversity loss, wetlands eutrophication, carbon emissions, and other farming stressors. Concomitantly, accompanying poor dietary patterns and malnutrition increase the risk for chronic diseases, such as cardiovascular diseases, obesity, type 2 diabetes, and cancer, which account for greater than 70% of global mortality per annum. Altogether, such annual monocropping systems exacerbate food insecurity, necessitating action across the fields of public health, agriculture, nutrition, medicine, and environmental ecology, that is, a transdisciplinary approach. Herein, we argue that the perennialization of crops creates an opportunity to address the challenges of environmental sustainability and nutritional adequacy economically. Unlike annuals, perennial crops have deeper roots for increased drought tolerance and reduced needs for fertilization and irrigation. Adopting perenniality can result in greater drought tolerance and improved soil health while reducing erosion, farming labor, and seed purchasing. Furthermore, perennializing novel staple crops may offer a superior and diverse dietary profile of phytochemicals, fiber, and macronutrients compared to conventional annuals. Instead of traditional perennial tree crops, we focus on intermediate wheatgrass Kernza® (Thinopyrum intermedium) and sunflowers (Helianthus tuberosus, H. maximiliani, and Silphium integrifolium) as exemplars of perennial staple food crops for grain and oil, respectively, at different stages of perennial crop commercialization. Ultimately, we discuss how integrating perenniality has the potential to revolutionize global agriculture and address food security concerns for the remainder of the 21st century.