Purpose: Needle-stick injury (NSI) is a major occupational hazard for transmission of blood-borne diseases among healthcare workers (HCWs). CDC estimated, annually around 236,000 out of 384,000 percutaneous injuries in the US are caused by NSI alone. This number is much higher in the developing countries. Neither standard reporting system nor any data on NSI is available from Bangladesh healthcare sector. This study was undertaken to survey the rate of NSI among HCWs and role of education and training in reducing NSI and improving occupational safety. Methods: A one year pretest-posttest study with intervention was conducted at a secondary level community hospital in Bangladesh. The study included a baseline survey, pretest-posttest with preformed questionnaire, intervention through classroom and hands-on training and a post-training survey. The compliance rate was assessed by covert observation using a checklist and data was analyzed by SPSS. Total 141 respondents participated in the study that included nurses, laboratory and cleaning staff. Results: After the training, overall NSI decreased significantly from 47.5% to 8.27% (p < 0.0001). NSI found to be reduced remarkably from 82.1% to 20.9% among cleaning staff, 32.3% to 4.1% among nurses and 88.2% to 15.3% among laboratory technicians (p < 0.0001). Other noteworthy findings were, introduction of colour-coded sharp bins facilitating segregation of needles from other wastes, development of protocols and use of personal protective equipments for cleaning staff, contributed largely in reduction of NSI. Conclusions: Findings of this study have emphasized the significance of education and training of HCWs in reducing NSI as it occurs mostly due to lack of knowledge and awareness about the consequences of NSI. This study also demonstrates that implementation of NSI reporting system, with proper segregation and disposal of sharps, facilitated quality improvement initiatives as well as safeguarding HCWs from NSI, which ultimately will improve patient outcomes and quality of care.