Seed availability poses a significant challenge for farmers, and True Shallot Seed (TSS) derived from seeds offers a potential solution to this problem. Currently, farmers primarily rely on bulb seeds for shallot cultivation. Therefore, extensive research on TSS seeds is crucial. This study aimed to investigate the impact of different indigenous FMA isolate types on the growth of various shallot varieties derived from seeds and the root infection of shallot plants. The research was conducted at the Wire House of the Faculty of Agriculture, Andalas University. A completely randomized design with two factors, namely the variety type and FMA dose, was employed, with three replications. The Lokananta and Sanren varieties were used, and the doses of FMA applied were 0, 10, 20, and 30 g/plant. The findings revealed no interaction between the variety type and FMA dose. The Sanren variety outperformed the Lokananta variety in plant height, number of leaves, percentage of root infection, root fresh weight, and root volume. Additionally, the FMA dose of 30 g/plant yielded better results regarding root fresh weight and volume.