Perccottus Glenii Dybowski Research Articles

Effect of selected environmental factors on the growth and survivability of the larvae of the Chinese sleeper Perccottus glenii Dybowski 1877

The aim of this study was to determine the influence of the temperature (range 15 to 25°C), pH (6 to 9) and salinity of water (between 0 to 10‰) on the growth, condition and survival of larvae of the Chinese sleeper. During the 28‐day experimental rearing, the highest average body weight of 81.3 ± 16.3 mg was reached in fish from the group maintained at a water temperature 25°C, and was almost five times higher than the weight at the temperature of 15°C (15.6 ± 2.2 mg). A significantly higher body weight of Chinese sleeper larvae was also recorded in the group reared in alkaline water (pH 9), compared to those kept in acidic and neutral water. Statistically significant differences between the groups (p≤0.05) were recorded for the condition index (K) values and relative body weight (SGR) only in the experiment with different water temperatures of fish rearing. The highest number (16% of the initial stock) of abnormal larvae, associated with a lower jaw and vertebral structure deformities, was recorded in the group reared at 5‰ salinity (Z5). Simultaneously, 100% mortality of the stock was noted by the 10th day of rearing in the 10‰ salinity. The cumulative mortality of the Chinese sleeper larvae related to cannibalism Types I and II in all the experimental rearing was low, oscillating between 0.5% and 11.5% in the groups bred in water with a pH 9 and 0‰ salinity, respectively. The study results may help determine the critical values of water parameters that promote or limit development in the early stages of the Chinese sleeper and could be used in activities related to eradicating or significantly reducing the increasing abundance of this alien invasive fish species in freshwater ecosystems.

Parasitic Helminths and Freshwater Fish Introduction in Europe: A Systematic Review of Dynamic Interactions

The introduction of non-native freshwater fish is a primary cause of aquatic biodiversity loss at global scale. Such introductions have a severe impact on freshwater ecosystems in terms of competition, predation, habitat alteration, genetic pollution and transmission of diseases and parasites. A systematic review was conducted on the helminths parasites of freshwater fish in the context of species introduction and a total of 199 publications were retrieved between 1969 and November 2022. Several scenarios may arise when a new fish species arrive in a recipient area. Non-native fish hosts can co-introduce their parasites without transmitting them to native fish (e.g., the case of North American Centrarchidae and their Monogenea parasites). Another possible outcome is the transfer of these parasites to the native fish fauna (spillover, e.g., the cases of the Nematoda Anguillicola crassus Kuwahara, Niimi & Itagaki, 1974 and the Monogenea Gyrodactylus salaris Malmberg, 1957). Reciprocally, non-native fish hosts may acquire parasites in their new distribution range whether these parasites are native or were previously introduced (e.g., the cases of Ponto-Caspian Gobiidae and the Chinese sleeper Perccottus glenii Dybowski, 1877). Acquired parasites can then be spilled back to the native fauna. This phenomenon is of particular interest when non-native fish hosts influence the dynamics of zoonotic parasites.

Correction: First record of highly invasive Chinese sleeper Perccottus glenii Dybowski, 1877 (Perciformes: Odontobutidae) in the Elbe River Basin, Czechia

Correction: First record of highly invasive Chinese sleeper Perccottus glenii Dybowski, 1877 (Perciformes: Odontobutidae) in the Elbe River Basin, Czechia

First record of highly invasive Chinese sleeper Perccottus glenii Dybowski, 1877 (Perciformes: Odontobutidae) in the Elbe River Basin, Czechia

First record of highly invasive Chinese sleeper Perccottus glenii Dybowski, 1877 (Perciformes: Odontobutidae) in the Elbe River Basin, Czechia

Intraguild predation as a potential explanation for the population decline of the threatened native fish, the European mudminnow (Umbra krameri Walbaum, 1792) by the invasive Amur sleeper (Perccottus glenii Dybowski, 1877)

Biotic interactions exerted by invasive species have a strong effect on ecosystems. Intraguild predation may contribute to the decline in the distribution, abundance and population size of native species and may facilitate the spread of non-native taxa. In this study, we investigated the feeding ecology of the invasive fish Amur sleeper (Perccottus glenii Dybowski, 1877) in a lowland watercourse, where it co-exists with the threatened native fish European mudminnow (Umbra krameri Walbaum, 1792). We used two sampling protocols that differed in the frequency of sampling time (e.g. monthly samplings and samplings in 10-day intervals) to provide evidence of predation, an interaction that may lead to the decline of mudminnow populations with the spread of the Amur sleeper. Aquatic macroinvertebrates comprised a major part of the diet for both sampling intervals. However, finer temporal resolution revealed the importance of fish, especially mudminnow juveniles, as a periodically available food source in the Amur sleeper’s diet. A high degree of dietary overlap was found between the different size groups of the Amur sleeper, but larger specimens tended to feed on a relatively higher proportion of fish. Our results suggested that temporal resolution of stomach content analyses may largely determine inferences on the importance of predation on juvenile mudminnow. Overall, we found that intraguild predation could contribute to the decline of European mudminnow populations, which underscores the importance of effective control measures to prevent the further spread of the invasive Amur sleeper.

Parasites of Perccottus glenii Dybowski, 1877 (Actinopterygii: Odontobutidae) in the native and the introduced host range: Abundance-occupancy and abundance-variance relationships.

The Chinese sleeper Perccottus glenii Dybowski, 1877 is an invasive fish species rapidly expanding in Siberia and Europe. Its native range encompasses the Far East region of Russia, northeastern China and northern North Korea. We studied species composition, prevalence, mean abundance and variance of mean abundance of macroparasites of the Chinese sleeper in the native and the introduced range. The species composition of the parasite component communities differed considerably in the native and the introduced range. The frequency distributions of prevalence, mean abundance and variance of mean abundance of the parasites did not demonstrate any significant differentiation between the two parts of the host range. However, an analysis of the abundance-occupancy and the abundance-variance relationships revealed that the parasite component communities in the two parts of the host range were quite distinct. In the native range, prevalence increased faster and variance increased more slowly with the increasing abundance of the parasites than in the introduced range. These features are mostly associated with considerably increased prevalence, abundance and aggregation of the host-specific cestode Nippotaenia mogurndae in recipient water bodies as compared with the native habitats.

Інвазивні види риб у річках басейну Західного Бугу в межах України

Purpose. To determine the occurrence of invasive fish species in ichthyofauna of rivers of the Western Bug basin in Ukraine. Methodology. Ichthiological studies were carried out during 2019-2021 in the Western Bug River and on its tributaries - Rata, Bushkov, Poltva and Mlynivka. Fish species identification was carried out in situ and was performed according to the identification keys of Yu.V. Movchan and A.F. Koblytska, incomplete biological analysis of invasive species was performed in the laboratory according to the methods of I.F. Pravdin, G.V. Nikolsky and I.I. Chugunova. Findings. The obtained data showed that invasive species in the composition of ichthyofauna in the studied rivers of the Western Bug basin were represented by five species: Prussian carp Carassius gibelio (Bloch, 1782), Stone moroko Pseudorasbora parva (Temminck & Schlegel, 1846), Pumkinseed Lepomis gibbosus (Linnaeus, 1758), Chinese sleeper Perccottus glenii Dybowski, 1877, Monkey goby Neogobius fluviatilis (Pallas, 1814). These species entered the ecosystem due to human activities through deliberate or accidental introduction into rivers of the Western Bug basin, where conditions are quite favorable for their vital activity and growth, due to a fairly warm climate and changes in hydrological regime of many rivers as a result of human impact. Originality. The article presents for the first time a list of invasive fish species of rivers of the Western Bug basin in Ukraine and provides a description of their habitats. Practical value. The obtained results can be used to regulate recreation fishery, for planning of conservation measures for rare fish species and for planning prevention of negative consequences from the spread of invasive fish species. Key words: ichthyofauna, the Western Bug River, invasive alien species, Prussian carp, Stone moroco, Pumkinseed, Chinese sleeper, Monkey goby.

Ecological status of fish fauna from Razim Lake and the adjacent area, the Danube Delta Biosphere Reserve, Romania

The aim of this study was to determine the ecological status of fish fauna of Razim Lake under the conditions of the water salinity changing from brackish, almost 70 years ago, into freshwater nowadays. The natural processes of siltation and organic deposits, characteristic of Danube Delta lake complexes, intensified in the last decades and included also Razim Lake. The presently reported study of Razim Lake and the adjacent area was undertaken in 2020 with intention to cover fish fauna collected with three different sampling methods (electrofishing, gillnetting, and seining). For each sampling method, Catch per Unit Effort (CPUE), relative abundance, and biomass were determined, as well as selected ecological parameters to determine ecological status of richness species in the area. Published data included 55 fish species, mainly marine and euryhaline, but in 2020 only 43 species were reported. Also, the species composition shifted from marine ones to freshwater or euryhaline ones. Of those 43 species captured in 2020 from Razim Lake and neighboring areas, 39 were native and four were non-native, including a newcomer, the Chinese sleeper, Perccottus glenii Dybowski, 1877. Few species were migratory, reophilous, or reophilous-stagnophilous which rarely enter Razim Lake, but the majority were limnophilous or stagnophilous-reophilous species. Four species were dominant in terms of the abundance; Blicca bjoerkna (Linnaeus, 1758); Rutilus rutilus (Linnaeus, 1758); Alburnus alburnus (Linnaeus, 1758); and Carassius gibelio (Bloch, 1782). In terms of the biomass the dominants were: Cyprinus carpio Linnaeus, 1758; Carassius gibelio; Sander lucioperca (Linnaeus, 1758); Pelecus cultratus (Linnaeus, 1758); and Blicca bjoerkna. Some differences between sampling methods used were observed. Eudominant, euconstant, and main species were Blicca bjoerkna and the majority of fish species were accessories, with differences amongst sampling methods used. Fish diversity parameters indicate a stable ichthyocoenosis, more stable along the lake shoreline. Ecological indicators of fish fauna from Razim Lake in 2020 grade the water lake quality as a moderate ecological class according to the Water Framework Directive of the European Union.

First report of the Chinese sleeper Perccottus glenii Dybowski, 1877 in the Drava River, Croatia

First report of the Chinese sleeper Perccottus glenii Dybowski, 1877 in the Drava River, Croatia

ИХТИОФАУНА НЕКОТОРЫХ ВОДОЁМОВ МЕСТНОГО ЗНАЧЕНИЯ АКСУСКОГО РАЙОНА, ПАВЛОДАРСКОЙ ОБЛАСТИ

In 2020, research works were carried out on seven reservoirs of local significance in Aksu district, Pavlodar region. These are Lake Komendant, Lake Krasnoe, Lake Lesnoe, Lake Topalevoe, Lake Kara Murza, Lake Baltazhar and the Karasu Channel. In these reservoirs, the ichthyological composition was studied, such fish species as pike (Esox lucius L., 1758), silver carp (C. gibelio (Bloch, 1782)), golden carp (Carassius carassius (L., 1758)), rotan (Perccottus glenii Dybowski), tench (Tinca tinca (L., 1758)), perch (Perca fluviatilis L. ,1758), roach (Rutilus rutilus (L., 1758)), carp (Cyprinus carpio L.,1758), and bream (Abramis brama (L., 1758)). The species of fish included in the Red Book of the Republic of Kazakhstan, as well as rare or endangered, are not marked in the research catches of the studied reservoirs.

Population genetic pattern of the freshwater fish Amur sleeper (Perccottus glenii) across its native distribution area in China

The Amur sleeper (Perccottus glenii Dybowski, 1877), which inhabits the Far East of Eurasia, is a fish capable of adapting to cold waters, including frozen water bodies. Therefore, it is a suitable vertebrate model for research on cryopreservation of entire organisms. However, the fish population has been decreasing annually and there is an urgent need to protect them. In this study, the genetic pattern in nine eco-geographical regions of Perccottus glenii from four water bodies across its native distributional area in China was explored by microsatellite markers. We found a high total genetic diversity (Ht: 0.4639) and a relatively low genetic diversity within populations (Hs: 0.1029). AMOVA revealed that 51% of the genetic variation occurred within individuals and 42% of the genetic variation occurred among populations. Among different sampled populations, high genetic differentiation (GST: 0.4024) was observed. Populations inhabiting Wusuli River, Fuyuan and Songhua River were found to be highly diverse, while populations inhabiting Hunchun and Ji’an were least diverse. A significant bottleneck was found in five populations and a significant Hardy-Weinberg Equilibrium was found in seven populations. Population structure analysis divided P. glenii populations into four clusters. The first cluster included the populations located downstream of the Amur River, the second cluster was comprised of the Liaohe River population and the populations located upstream and in the middle of the Amur River. The third and fourth clusters were composed of populations located in the Tumen River and Yalu River, respectively. These findings indicate that the conservation management of P. glenii is urgent.

The role of invasive Chinese sleeper Perccottus glenii Dybowski, 1877 in the Ilgas Nature Reserve ecosystem: an example of a monospecific fish community

The role of invasive Chinese sleeper Perccottus glenii Dybowski, 1877 in the Ilgas Nature Reserve ecosystem: an example of a monospecific fish community

A checklist of parasites of non‐native populations of the fish rotanPerccottus glenii(Odontobutidae): Communication II

The fish rotan, Perccottus glenii Dybowski, 1877 (Odontobutidae) is one of the most successful fish invaders during 20th and beginning of 21st century in European and Asian fresh waters. The diversity of its parasites within its invaded range was first reviewed by Sokolov, Reshetnikov, et al. (2014). The current paper analyzes new data from 2013 to 2019 on protistan and metazoan parasites of the non-native populations of this fish. We used information from peer-reviewed journals, difficult to access grey literature and our own recently obtained original data. 45 literature sources were included in the analysis. The newly-available data originate from 95 water bodies of 13 river basins, isolated lakes in West Siberia, and Gulf of Finland, spanning a total of eight countries. A total of 132 recently detected parasite species/taxa are listed in this paper. Each species is presented with its location in/on the host, geographical localities, countries, and according literature sources. When available, number of examined fish as well as prevalence and range of intensity of infection are cited from the original source. This review enlarges contemporary knowledge on parasites of non-native populations of the studied fish species up to 167 species/taxa. We highlight perspectives for further parasitological investigations of this invasive fish.

Effects of Roundup Herbicide at Low Concentration and of Thermal Stress on Physiological and Biochemical Parameters in Amur Sleeper Perccottus glenii Dybowski Juveniles

The effects of 30-day exposure to Roundup at concentration of 2 μg/L and of thermal stress on the intestinal glycosidase activities (maltase and amylolytic activities (AA)), brain acetylcholinesterase (AChE) activity and water-soluble protein (WSP) content in juvenile specimens of Amur sleeper Perccottus glenii Dybowski have been studied. Additionally, critical thermal maximum (CTM) and final selected temperature (FST) are determined in fish of control (exposure to 0 μg/L Roundup) and Roundup-exposed groups. After Roundup exposure AA and WSP content were 27 and 31% lower respectively, maltase and AChE activities did not change. An increase in water temperature at a rate of 8°C/h decreased AA by 60%, maltase activity by 18% and WSP content by 28% in fish of the control group; but decreased AA by 76%, AChE activity by 29%, WSP content by 40%, and increased maltase activity by 11% in fish of the Roundup-exposed group. The recovery treatment during 12 days in a thermal gradient with herbicide-free water (temperature range between 14 and 31°C) reduced the inhibitory effect of heating on AA, but increased it the on AChE and WSP content in fish from both control and Roundup-exposed groups (on maltase activity only in the fish of exposed group). No significant differences were noticed in CTM and FST values in fish of control and Roundup-exposed groups.

The Phylogenetic Position of Spironucleus sp. (Diplomonadida: Hexamitidae) from the Intestine of Chinese Sleeper Perccottus glenii Dybowski, 1877 (Actinopterygii: Odontobutidae).

The Chinese (Amur) sleeper (Perccottus glenii Dybowski, 1877) (Actinopterygii: Odontobutidae) is a freshwater fish species with high invasive potential. Diplomonads have been detected in the intestines of Chinese sleepers using light microscopy. The aim of this study was to identify the diplomonads in Chinese sleepers using molecular-genetic methods. The fish used in this analysis were caught in the following bodies of water in Russia between 2014 and 2016: Lake Dolgoe, the floodplain of the Ingoda River (Amur River basin), the Tsna River (the Oka River basin), and the littoral of the Kotlin Island (Gulf of Finland). Partial sequences of small subunit rRNA genes were obtained for the intestinal diplomonads of Chinese sleeper. The analysis of all sequenced samples revealed the presence of Spironucleus salmonis Moore, 1922; other Spironucleus species were not found in the sampled fish. With 82% probability, the sampled sequences of diplomonads from Chinese sleeper formed a separate cluster in the clade of S. salmonis on the phylogenetic tree. This is the first record of S. salmonis in fish in the family Odontobutidae.

Myxidium shedkoae Sokolov, 2013 (Myxozoa: Myxidiidae), a parasite of the gallbladder of Perccottus glenii Dybowski, 1877 (Actinoptrygii: Odontobutidae): Supplementary data on morphology and phylogenetic position based on 18s rDNA sequence analysis.

This paper is the first report on the molecular characterisation of myxozoan parasites from the odontobutid fish Chinese (Amur) sleeper (Perccottus glenii Dybowski, 1877). The authors determined the partial 18S rDNA sequence of Myxidium shedkoae Sokolov, 2013 from the gallbladder of the fish. Phylogenies reconstructed using maximum likelihood and Bayesian inference analysis revealed that M. shedkoae belongs to the hepatic biliary group of myxozoans (after Kristmundsson and Freeman, 2013) as a member of the clade consisting of Zschokkella sp. KLT-2014, Myxidium truttae and Zschokkella nova. Some new morphological features of the parasite are also presented.

The Phylogenetic Position of the Cestode Nippotaenia mogurndae Yamaguti et Miyata, 1940 (Cestoda: Nippotaeniidae), a Parasite of the Chinese Sleeper Perccottus glenii Dybowski, 1877 (Actinopterygii: Odontobutidae), Based on a Partial Sequence of the 18S rRNA Gene

A partial sequence of the 18S rRNA gene (1979 bp) of the cestode Nippotaenia mogurndae Yamaguti et Miyata, 1940, a parasite of the Chinese sleeper Perccottus glenii Dybowski, 1877, is obtained. The maximum likelihood (ML) and Bayesian Inference (BI) algorithms are used for phylogenetic analysis that included the sequence and data obtained from GenBank. Both algorithms reveal a close phylogenetic relationship of our N. mogurndae and the N. mogurndae s. str. from its type host, Odontobutis obscura (Temminck et Schlegel, 1845), from Japan. The genetic divergence between these helminths (0.005) corresponds to the level of intraspecies differences typical for groups of cestodes phylogenetically close to the nippotaeniids: Mesocestoididae and Hymenolepididae. The results of the phylogenetic analysis also do not support the separation of nippotaeniid cestodes into two genera: Amurotaenia Achmerow, 1941 and Nippotaenia Yamaguti, 1939.

Effects of Roundup Herbicide and Increase in Water Temperature on the Parameters of Peripheral Blood Cells in Amur Sleeper Perccottus glenii Dybowski

The separate and combined effects of chronic 30-day exposure to the herbicide Roundup in a sublethal concentration of 2 μg/L and an increase in water temperature at a rate of 8°C/h on the parameters of red and white blood in juveniles of Amur sleeper Perccottus glenii Dybowski have been studied. The ratio of mature and immature erythrocytes in the peripheral blood do not change under the influence of the studied factors. An increase in temperature after chronic exposure to Roundup leads to a decrease in red blood cell sizes and increase in the share of abnormal cells. Exposure to the herbicide and the rise in water temperature have the opposite effect on the number of amitosis in erythrocytes and the ratio of leucocyte cells; an antagonistic effect is identified under the combined action of the factors. Changes in white blood correspond to a nonspecific stress response; changes in red blood indicate a reduction in compensatory responses to hypoxia.

The Chinese sleeper (Perccottus gleniiDybowski, 1877) in Ukraine: New data on distribution

The Chinese sleeper Perccottus glenii Dybowski, 1877 is one of the most successful alien species in Europe today. This article is a review of the current distribution of the species in Ukraine using published data, own field studies (2005–2016) and collections from museums and colleagues. The Chinese sleeper has been confirmed in the Vistula, Danube, Dniester, Southern Bug and Dnipro river basins, with earlier occurrence (1970s–90s) in the Vistula, Danube and Dniester river basins. As a result, the species is distributed more evenly in these river basins. The Chinese sleeper was first recorded in the Dnipro and Southern Bug river basins in the 21st century; hence, its occurrence is still irregular. Present data suggest that, in some regions, the species could inhabit not only floodplains and bays but also fluvial river sections.

The diversity of parasites in the Chinese sleeper Perccottus glenii Dybowski, 1877 (Actinopterygii: Perciformes) under the conditions of large-scale range expansion

It has been found that the species composition of parasites infesting the Chinese sleeper Perccottus glenii in water bodies from the nonnative part of its range is more depleted. Here, the phylogenetic distances between parasites exceed those in the native part of the range. It has been revealed that parasitological differences between P. glenii populations from the nonnative and native parts of the range, as well as between populations inside the nonnative part, have similar composition and abundance of the host-specific and euryxenous components in the parasitic fauna. It has been shown that these differences are determined by the genesis of sites from which P. glenii is introduced, as well as the local conditions of the recipient water bodies.