Event Abstract Back to Event Ontogenetic stomach development in catshark Scyliorhinus canicula. Odete Goncalves1, 2*, Filipe Castro1*, Renata Freitas3, Patricia Ferreira1, 2, Ana Aguas2, 3, Fabio Barroso2, 3, Sylvie Mazan4, João Coimbra1, 2 and Jonathan M. Wilson1, 5* 1 CIIMAR, Portugal 2 ICBAS, Portugal 3 IBMC, Portugal 4 Station Biologique de Roscoff, France 5 Wilfrid Laurier University, Canada The gastrointestinal tract (GIT) of vertebrates develops from a simple undifferentiated tube into highly differentiated regions for the digestion and absorption of nutrients. Each region has a different histological structure and gene expression profile that enables the performance of their different functions. The stomach is the most highly derived GIT region and gastric glands are responsible for the secretion of HCl and pepsinogen that enables acid-peptic digestion, representing a functional innovation found exclusively in jawed vertebrates. The mechanisms of GIT organ specification are well studied in some vertebrates including mouse, Xenopus, chicken and some teleost fishes. General embryonic development has been studied in chondrichthyans but not GIT development. In this study we characterize the development of the stomach in the catshark Scyliorhinus canicula, an elasmobranch with a ~5 month development spanning 34 developmental stages. As a representative of basal jawed vertebrate for which genomic information is currently available, the catshark is a suitable model system to characterize the ancient expression and function developmental of physiologically relevant genes. Therefore, during this study we used embryos from a brood stock held in our fish facility to conduct gene expression, protein detection and histological analyses. Our aim was to characterize, at molecular and morphological levels, stomach development and the differentiation of its characteristic secreting glands. The differentiation of the stomach starts around stage 24. However, immunohistochemistry for the gastric proton pump indicates a delayed development of gastric gland that are only visible before hatching at stage 33-34. Supported by NSERC to JMW Acknowledgements BOGA-CIIMAR FCT (grant BD/79821/2011) NSERC (Canada) References Ballard WW, Mellinger J, Lechenault H (1993) A series of normal stages for development of Scyliorhinus canicula, the lesser spotted dogfish (Chondrichthyes; Scyliorhinidae). J Exp Zool. 267:318–3362 Freitas, R, Zhang GJ and Cohn, MJ (2006). Evidence that mechanisms of fin development evolved in the midline of early vertebrates. Nature 442, 1033-1037. Castro L.F.C., Goncalves O, Mazan S, Tay B-H, Venkatesh B, Wilson JM (2014). Recurrent gene loss correlates with the evolution of stomach phenotypes in gnathostome history. Proceedings of the Royal Society B-Biological Sciences 281: 1775. Keywords: Stomach, Development Studies, vertebrate, Chondrichthyes, Immunohistochemistry Conference: XV European Congress of Ichthyology, Porto, Portugal, 7 Sep - 11 Sep, 2015. Presentation Type: Poster Presentation Topic: Physiology, Behavior and Toxicology Citation: Goncalves O, Castro F, Freitas R, Ferreira P, Aguas A, Barroso F, Mazan S, Coimbra J and Wilson JM (2015). Ontogenetic stomach development in catshark Scyliorhinus canicula.. Front. Mar. Sci. Conference Abstract: XV European Congress of Ichthyology. doi: 10.3389/conf.FMARS.2015.03.00261 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 31 Dec 2015; Published Online: 31 Dec 2015. * Correspondence: MD. Odete Goncalves, CIIMAR, Porto, Portugal, odete007@gmail.com PhD. Filipe Castro, CIIMAR, Porto, Portugal, lfilipecastro@gmail.com Dr. Jonathan M Wilson, CIIMAR, Porto, Portugal, wilson.jm.cimar@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Odete Goncalves Filipe Castro Renata Freitas Patricia Ferreira Ana Aguas Fabio Barroso Sylvie Mazan João Coimbra Jonathan M Wilson Google Odete Goncalves Filipe Castro Renata Freitas Patricia Ferreira Ana Aguas Fabio Barroso Sylvie Mazan João Coimbra Jonathan M Wilson Google Scholar Odete Goncalves Filipe Castro Renata Freitas Patricia Ferreira Ana Aguas Fabio Barroso Sylvie Mazan João Coimbra Jonathan M Wilson PubMed Odete Goncalves Filipe Castro Renata Freitas Patricia Ferreira Ana Aguas Fabio Barroso Sylvie Mazan João Coimbra Jonathan M Wilson Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.