Owing to their comfort, handle, and aesthetic characteristics, cotton fabrics will always be the first and primary choice for clothing and apparel. In recent years, regenerated cellulosic fabrics like bamboo, Tencel, and modal fabrics have had many natural advantages. Fabrics based on blending cotton fibres with regenerated cellulosic fibres are considered promising products in textile industry sectors. Use of urea poses ecological problems associated with the high nitrogen content of the printing effluent. Therefore, urea reduction or elimination in reactive dye print pastes is of ecological interest. We report the use of trisodium nitrilotriacetate as a complete substitution of urea and alkali in the conventional reactive printing of cotton/cellulosic regenerated blended fabrics. CI Reactive Black 5 was selected for the present study. Three different print pastes containing urea/alkali, trisodium nitrilotriacetate/alkali and trisodium nitrilotriacetate without alkali were thoroughly investigated. Different factors that may affect the printability of cotton/cellulosic regenerated blended fabrics, such as the concentrations of dye, trisodium nitrilotriacetate, urea, absence or presence of alkali and steaming time in the prints obtained, were evaluated concerning colour strength, dye fixation, dye penetration, levelling, colure, and fastness properties. All printed fabrics using three print pastes obtained excellent to good fastness. The results proved the viability of using TNA as an environmentally friendly approach for urea/alkali-free printing of cellulosics with reactive dyes.