Dye Penetration Research Articles

The Effect of Different Obturation Techniques on the Sealing Ability of Root Canal Filling Materials

ABSTRACT Background: The performance of root canal filling materials in sealing is crucial for the effectiveness of endodontic procedures as it prevents the escape of microorganisms and guarantees the durability of the treatment. Materials and Methods: The present in vitro investigation included the preparation of 90 human single-rooted teeth using a standardized process. These teeth were then randomly separated into three groups (n = 30) according to the obturation technique: Group A (Lateral Compaction), Group B (Thermafil), and Group C (Continuous Wave of Condensation). Furthermore, each group was separated into two subgroups based on the specific kind of root canal filling material employed: Subgroup 1 (Gutta-Percha) and Subgroup 2 (Resilon). The sealing capacity was evaluated using a dye penetration technique, with measurements conducted at three specific time intervals: 24 hours, 1 week, and 4 weeks after the obturation process. Statistical analysis of the data was conducted using analysis of variance and post hoc tests to identify significant differences across groups. Results: The findings showed that the Continuous Wave of Condensation method (Group C) had the lowest dye penetration, with an average depth of penetration of 1.2 mm. The Thermafil technique (Group B) followed closely with a penetration depth of 1.8 mm, while the Lateral Compaction technique (Group A) achieved a penetration depth of 2.5 mm. In terms of sealing ability, Gutta-Percha demonstrated superior performance compared to Resilon, achieving average penetration depths of 1.5 mm and 2.0 mm, respectively. Statistical analysis showed statistically significant differences between the groups (P < 0.05), with the Continuous Wave of Condensation approach utilizing Gutta-Percha showing the highest level of sealing effectiveness. Conclusion: In conclusion, the Continuous Wave of Condensation approach showed a substantial improvement in the sealing efficacy of root canal fillings when compared to the Lateral Compaction and Thermafil procedures.

Does different application modes of universal adhesives with universal resin composites affect the microleakage in class V cavities? An in vitro study.

Composite restorations often have gingival margins near the cemento-enamel junction (CEJ), where the microleakage of these margins can significantly contribute to the restoration failure, especially in the cervical lesions. It is important to determine the microleakage is crucial, as it typically occurs through the interfacial gap between the tooth and the restoration. Various resin composites and techniques have been developed to minimize this gap and reduce the risk of microleakage. The aim of this in vitro study was to evaluate the levels of microleakage of different modes of a universal adhesive and two novel resin composites in restoring class V cavities in the central incisors. Sixty-six freshly extracted sound human central incisors of the similar size were randomly assigned to 2 groups (n = 33 per group) according to the brand of resin composite. Each group was further divided into 3 subgroups based on the Scotch Bond Universal (3M ESPE, Saint Paul, MN, USA) application protocol used: (a) total etch, (b) self-etch and (c) selective etch. After composite restoration completed with Omnichroma (Tokuyama Dental Corp., Tokyo, Japan) and Filtek Universal Restorative (3M ESPE, Saint Paul, MN, USA), each tooth was immersed in a 0.5% basic fuchsin dye solution at 37C0 for 24h. After dye penetration, teeth were sectioned and evaluated with conventional (scoring) and digital methods (ImageJ). The intra- and inter-examiner agreement was estimated according to the Kappa statistics and the results were analyzed with the one-way ANNOVA and the Kruskal-Wallis test (p < 0.05). The rates of microleakage of the gingival and incisal margins are statistically similar, regardless of the composite brand and the method of application of the universal adhesive. The microleakage exhibited by current adhesives and resins is independent of the adhesive application mode and measurement method.

Effect of Silver Diamine Fluoride and Potassium Iodide on Microleakage of Composite Resin in Anterior Primary Teeth.

One of the preventive interventions for early childhood caries is the use of silver diamine fluoride (SDF), which works uniquely in the prevention of caries. The suggested method to minimize the discoloration of SDF is the use of potassium iodide (KI). However, there are concerns about the bonding properties of dentin after the application of SDF-KI. Therefore, this study aimed to investigate how SDF-KI affects composite resin microleakage in primary teeth. In this in vitro study, class V cavities were created on the buccal surfaces of 60 extracted primary canines. The samples were divided into three groups: Group I: 38% SDF solution was applied to the cavity; Group II: SDF-KI solution was applied to the cavity; and group III: the cavities were irrigated with distilled water. All cavities were filled with composite resin and subjected to a thermocycling regime (500 cycles). The dye penetration of samples was evaluated following a 24-h immersion in 1% methylene blue. Microleakage at the occlusal and gingival margins was evaluated using a stereomicroscope at 40× magnification. Data were analyzed using the Chi-squared test (p < 0.05). There was no statistically significant difference between microleakage scores at the occlusal margin (p = 0.128). At the cervical margin, the SDF-KI group had significantly more microleakage than the SDF and control groups (p = 0.001). Applying the SDF-KI significantly increased microleakage in the cervical margin but had no significant effect on the occlusal margin. Regarding the beneficial effects of SDF in preventing and arresting dental caries, application of it before composite resin is suggested in controlling caries without the negative effects on microleakage. How to cite this article: Meraji A, Asadian F, Hekmatfar S. Effect of Silver Diamine Fluoride and Potassium Iodide on Microleakage of Composite Resin in Anterior Primary Teeth. J Contemp Dent Pract 2024;25(7):691-695.

A novel green approach for reactive printing of cotton/cellulosic regenerated blended fabrics using trisodium nitrilotriacetate

Owing to their comfort, handle, and aesthetic characteristics, cotton fabrics will always be the first and primary choice for clothing and apparel. In recent years, regenerated cellulosic fabrics like bamboo, Tencel, and modal fabrics have had many natural advantages. Fabrics based on blending cotton fibres with regenerated cellulosic fibres are considered promising products in textile industry sectors. Use of urea poses ecological problems associated with the high nitrogen content of the printing effluent. Therefore, urea reduction or elimination in reactive dye print pastes is of ecological interest. We report the use of trisodium nitrilotriacetate as a complete substitution of urea and alkali in the conventional reactive printing of cotton/cellulosic regenerated blended fabrics. CI Reactive Black 5 was selected for the present study. Three different print pastes containing urea/alkali, trisodium nitrilotriacetate/alkali and trisodium nitrilotriacetate without alkali were thoroughly investigated. Different factors that may affect the printability of cotton/cellulosic regenerated blended fabrics, such as the concentrations of dye, trisodium nitrilotriacetate, urea, absence or presence of alkali and steaming time in the prints obtained, were evaluated concerning colour strength, dye fixation, dye penetration, levelling, colure, and fastness properties. All printed fabrics using three print pastes obtained excellent to good fastness. The results proved the viability of using TNA as an environmentally friendly approach for urea/alkali-free printing of cellulosics with reactive dyes.

Flexible assessment of line polymer insulators based on condition indexes

Line polymer insulators, as a kind of power system equipment which are faced with many of the environmental stresses, need to have a condition assessment strategy with the aim of predictively protection of lines against any failures and outages. This paper, according to some experimental test process results, proposed a flexible condition assessment method based on defined values of indexes which consist of elongation percent, hardness, leakage current, corrosion, dye penetration, water diffusion, thermo-gravimetric analysis and Fourier transform infrared spectroscopy with regard to visual inspection and hydrophobicity classification. Also, estimations of operation life time have been done for unknown-years-old insulators of the Yazd utility of Iran power network as a case study by defining relative factors. Finally, with regard to environmental pollution measurement, by considering the assessment results, a series of next actions have been suggested according to lines importance.

How Irrigants Affect Mineral Trioxide Aggregate (MTA) Sealing in Furcal Perforations: An In Vitro Study.

Objective This study aimed to evaluate the effect of 5.25% sodium hypochlorite (NaOCl) and 2% chlorhexidine (CHX) on ProRoot mineral trioxide aggregate (MTA) used for treating furcal perforations, compared to no solution application, using the dye penetration (DP) method. Materials and methods The study included 36 intact mandibular molars in the furcal area (FA) with well-spaced roots. Using dental operating microscopes, perforations were created at the FA of the molars with a 1.2 mm diameter. Subsequently, ProRoot MTA (Dentsply, Germany) was applied to seal the perforations. After 24 hours, the samples were randomly assigned to three groups: Group A (n = 12), irrigated with 5.25% NaOCl; Group B (n = 12), irrigated with 2% CHX; and Group C (n = 12), without irrigation (control group). Specimens' pulp chambers were immersed in 2% methylene blue for 24 hours. Buccal-lingual sections were performed, and the depth of marginal dye leakage was assessed using a stereomicroscope at 20x magnification. Data analysis employed the one-way analysis of variance (ANOVA) test and Bonferroni post-hoc test (α = 0.05). Results The study revealed significant differences in DP values between groups (p-value < 0.01). DP values in the control group (no irrigation) were lower than both NaOCl (p < 0.05) and CHX (p < 0.01) groups. Furthermore, DP values in the CHX group were higher than those in the NaOCl group (p < 0.01), suggesting the order: no irrigation < NaOCl < CHX (p < 0.05). Conclusions The sealing ability of MTA was compromised when irrigated with 2% CHX and 5.25% NaOCl. CHX significantly impacted the marginal leakage and sealing ability of MTA. Care should be taken when resuming endodontic treatment after the application of MTA in cases of furcal perforations as the use of CHX and NaOCl may affect the marginal leakage of MTA.

Assessment of Mechanical Strength of A36 Steel with Flux Cored Arc Welding Process

Shielded Metal Arc Welding (SMAW) is a popular welding technology in the construction and industrial sectors due to its ease of use. Flux-cored arc welding (FCAW) has gained popularity in the construction sector and industrial settings. The Welding Procedure Specification (WPS) restricts material compatibility for FCAW due to systematic selection and qualification. Penetrant Testing exposes damage to welded specimens, ensuring they meet the standard. Non-Destructive Testing (NDT) and mechanical tests like tensile and bending tests determine the mechanical strength and weld characteristics of steel using FCAW. The results show the effectiveness of pWPS and satisfy the code and standard. Dye Penetrant Test results show no cracks and acceptable criteria for both side A and side B of the steel plate. Bending test results show expected yield points, but failures occur due to discontinuities in break force and failures in tensile strength.

Evaluation of microleakage of mineral trioxide aggregate and biodentine as apical barriers in simulated young permanent teeth

BackgroundApexification is a procedure that promotes apical closure by forming mineralized tissue in the apex region of a nonvital young permanent tooth. Calcium silicate-based cement like Mineral trioxide aggregate (MTA) and Biodentine are commonly employed as apical barriers to facilitate this process. Microleakage, defined as the leakage along the junction between the canal wall and filling material, is a crucial aspect to assess in MTA and Biodentine applications as apical barriers, as it directly impacts the prevention of bacterial seepage and maintenance of structural integrity. The current study aims to assess the microleakage of MTA and Biodentine when used as apical barriers in simulated young permanent teeth.MethodsFrom a total of 128 extracted teeth, 114 were selected for the study and randomly allocated into three groups: G1 (MTA), G2 (Biodentine), and G3 (Control), with 38 teeth per group. After excluding 5 teeth from each group due to issues such as canal calcification, breakage during sectioning, and procedural errors, 33 teeth were analyzed to ensure equal distribution. To simulate young permanent teeth, samples were instrumented using a person-reamer with a diameter of 1.7 mm. A 4 mm thick apical plug of MTA and Biodentine was placed in G1 and G2, respectively, while G3 was the control group. Apical microleakage in all experimental groups was assessed using a dye penetration method. Specimens were longitudinally sectioned and examined under a stereomicroscope with graded eyepiece.ResultsThe Kruskal-Wallis test revealed variations in mean apical microleakage among the groups: G1 recorded 0.67, G2–0.16, and G3–1.62, with G2 showing the lowest value and G3 group exhibiting the highest.ConclusionsBiodentine was found to excel in its ability to create a secure seal and function effectively as an apical barrier in simulated young permanent teeth. These results underscore its potential as a highly efficient material for dental applications, particularly in scenarios requiring reliable sealing and barrier formation in the root canal system of developing permanent teeth.

Quantitative assessment of early caries lesion activity using novel dye-enhanced fluorescence imaging

ObjectivesThis study aimed to evaluate the validity of the dye-enhanced quantitative light-induced fluorescence (DEQLF) method for assessing early enamel caries activity. MethodsSeventy extracted human teeth with early enamel caries on smooth surfaces were included. Two dentists evaluated the caries activity using the Nyvad system followed by the DEQLF method. The teeth were hydrated with distilled water for 60 s, dehydrated with compressed air for 10 s, and stained with 100 μM fluorescein sodium solution for 10 s. White and fluorescent images were captured using a QLF-D 2+ Billuminator. The change in fluorescence (ΔΔG) was calculated using image analysis software. Independent-sample t-tests were performed to evaluate the difference in ΔΔG between active and inactive lesions for both DEQLF and conventional quantitative light-induced fluorescence (QLF) methods. Receiver operating characteristic (ROC) curve analysis was used to assess the validity of ΔΔG for distinguishing lesion activity using the area under the ROC curve (AUROC). ResultsAmong the 70 caries lesions, 33 were active and 37 were inactive. Using the DEQLF method, the ΔΔG for active lesions (3.8 ± 5.6) was significantly higher than that for inactive lesions (1.0 ± 2.5) (P < 0.05). With the conventional QLF method, there was no significant difference in ΔΔG between active (-1.1 ± 1.7) and inactive (-1.3 ± 1.7) lesions. DEQLF-derived ΔΔG demonstrated an AUROC of 0.72, a sensitivity of 0.67, and a specificity of 0.76. ConclusionsApplying the DEQLF method to human teeth enabled the quantitative assessment of lesion activity based on dye penetration. DEQLF-derived ΔΔG values showed significant differences based on lesion activity status and demonstrated high validity in distinguishing lesion activity. Clinical significanceClinicians can use the DEQLF method, which involves applying a fluorescent dye for 10 s prior to conventional QLF, to objectively quantify and distinguish the activity status of early enamel caries, potentially replacing the traditional reliance on subjective visual assessments.

Comparative evaluation of coronal sealing ability among commercially available three endodontic temporary restorative materials by using methylene blue dye penetration test : an in-vitro study

Aim: To evaluate and compare the coronal sealing ability among commercially available three endodontic temporary restorative materials by using methylene blue dye penetration test. Methods and Material: 90 extracted mandibular and maxillary premolar teeth were taken. Samples were divided into four experimental groups of 20 each (n-20). In the experimental group, endodontic access cavity was created, and in each group, then the teeth were restored with Orafil G, Coltosol F and IRM. A positive control group was taken (n -5), access cavities were prepared, but not resored. A negative control group was taken (n-5), access cavities were not prepared. Samples were placed in normal saline for 2 hours and thermocycled at 5 ± 2ºC to 55 ± 2ºC for 30 seconds for 100 cycles. Then samples were immersed in methylene blue dye for 7 days. Longitudinal sectioning (mesiodistally) of samples were done with diamond disc and dye penetration was graded using a stereomicroscope at 10 x magnification. Statistical analysis used: The statistical analysis used was Kruskal-Walli’s test and one way ANOVA test to compare the Microleakage. Results: Microleakage was observed in Orafil G followed by Coltosol F. No significant difference was seen between two groups of IRM.Conclusions: Orafil G showed considerably good results compare to Coltosol F and IRM

Effect of adding sodium fluoride and nano-hydroxyapatite nanoparticles to the universal adhesive on bond strength and microleakage on caries-affected primary molars.

Evaluation of micro tensile bond strength (μTBS) and marginal leakage of sodium fluoride (NaF) and nano-hydroxyapatite (n-HA) modified universal adhesives (UAs) bonded using etch-and-rinse (ER) and self-etch (SE) bonding technique to the carious affected dentin (CAD). One hundred and twenty primary molars were prepared for CAD on the occlusal surface. The occlusal CAD surface was flattened and underwent a polishing procedure. The specimens were divided into six groups using a random allocation method based on the UAs applied and the mode of etching used (n = 20) Group A1: UAs (ER), Group B1: UAs (SE), Group A2: UAs (NaF) + ER, Group B2: UAs (NaF) + SE, Group A3: UA (n-HA) + ER and Group B3: UAs (n-HA) + SE. Composite restoration was placed and samples were thermocycled. Microleakage, μTBS, and failure mode assessment were performed using a dye penetration test, universal testing equipment, and stereomicroscope respectively. The μTBS and microleakage results (mean ± SD) were examined using analysis of the variance (ANOVA) and Tukey post hoc tests. Group B1 (UAs + SE) demonstrated the maximum scores of microleakage (25.14 ± 9.12 nm) and minimum recorded value of μTBS (14.16 ± 0.55 MPa). In contrast, Group A3 (UAs (n-HA) + ER) displayed a minimum value of marginal leakage (12.32 ± 6.33 nm) and maximum μTBS scores (19.22 ± 0.92 MPa). The outcomes of the intergroup comparison analysis showed that Group A2 (UAs (NaF) + ER), Group B2 (UAs (NaF) + SE), Group A3 (UA (n-HA) + ER) and Group B3 (UAs (n-HA) + SE) presented comparable outcomes of marginal seal outcomes and μTBS scores (p > 0.05). NaF and n-HA-modified UAs displayed favorable bond strength and minimum marginal leakage to the deciduous affected dentin surface.

Comparative evaluation of marginal integrity of three esthetic restorative materials in class V cavities under stereomicroscope: an in-vitro study

Aims: The aim of this invitro study was to compare the marginal integrity among three esthetic restorative materials. Methods and Material: This study was conducted on 60 freshly extracted human premolars in which Standardized Class V cavities were prepared. Teeth were equally assigned to three groups : GC- Aenial Anterior Composite(Group A), Tetric N- Ceram(Group B), Palfique LX5(Group C). Each group containing 20 teeth were restored and then stored in distilled water for 1 week at 37°C. The specimens were dried and painted with two coats of nail varnish on the teeth except 2mm around the cervical margins of the restoration and air dried. After that the teeth were kept in 2 % methylene blue solution for 24 hours. After sectioning the teeth, the sectioned halves were then evaluated for dye penetration under stereomicroscope. Statistical analysis used: The statistical analysis was performed using the Kruskal-Wallis test along with post hoc pairwise comparison by Mann Whitney U test. Results: The findings suggested that Tetric N-Ceram was superior to the other two composite materials Conclusions: Marginal leakage scores of novel restorative material, Tetric N- Ceram was minimal and within acceptable limits compared to other tested material.

Carbon nanotube-modified adhesive to caries affected dentin conditioned with Nd: YAP laser, phosphoric acid, and photoactivated-erythrosine

AimDentin conditioned with phosphoric acid (PA) Nd: YAP laser, and photoactivated-Ery(Erythrosine) on microleakage, shear bond strength (SBS) degree of conversion (DC), and rheological assessment of adhesive-infused with carbon nanotubes (CNTs). Material and methodCarious ninety-six human mandibular molars were included. Specimens were disinfected and allocated into three groups based on surface pretreatment (n = 32) Group 1 (PA), Group 2 (Nd: YAP) laser, and Group 3 (Photoactivated-Ery). Conditioned groups were further divided into two 2 subgroups based on the application of unmodified ERA and CNTs-modified ERA. Composite restorations were placed on the CAD surface and thermal aging of the samples was performed. The microleakage assessment was conducted using a dye penetration test. Universal testing machine (UTM) assessed SBS bond failure was evaluated using a stereomicroscope. Scanning electron microscopy (SEM), energy-dispersive X-ray (EDX) analyses of CNT, and Fourier Transform Infrared FTIR of adhesives were performed. One-way ANOVA and Tukey post hoc analyzed the outcomes. ResultsGroup 1B samples (PA+ CNTs modified adhesive) presented the minimum marginal leakage and highest bond integrity. Group 2A (Nd:YAP laser+Unmodified adhesive) displayed the maximum scores of microleakage and lowest bond strength. ConclusionPhotoactivated Ery-PS can serve as an alternative to phosphoric acid for conditioning CAD. Incorporating CNT in adhesive significantly enhanced bond integrity and marginal seal with no significant difference in DC.

Microleakage Assessment of Single Cone Gutta Percha Obturation Technique Using Different Types of Sealers

This study was carried out to compare the coronal and apical seal of canals obturated with single cone technique using different types of epoxy resin root canal sealers. A total of sixty extracted human upper molars with fully formed and sound palatal roots were used in this study. The crown of each tooth was decoronated at cement-enamel junction and the palatal root canal was prepared using ProTaper gold rotary instruments. Sixty specimens were used and divided into 3 equal groups(n=20) for single cone obturation using two different types of epoxy resin sealers AH26 and vioseal sealers respectively and one control group without sealer. All the specimens were exposed varnish application followed by dye penetration test using 2% methylene blue dye then root sectioning. Samples were evaluated under a stereomicroscope to detect the linear measurement of the dye penetration from the apical constriction. The image transferred to a computer equipped with the image analysis software program where the dye penetration along the filling dentine interface was evaluated. The collected data was statistically analysis by using Graph Pad Instat software for windows. One-way ANOVA was done for compared results followed by Tukey’s pair-wise. The results showed that, the difference in dye penetration microleakage means between groups was statistically significant as revealed by one-way ANOVA test (p &lt; 0.05). Pair-wise Tukey’s post-hoc test showed non-significant (p&gt;0.05) difference between (AH26 and Vioseal sealers) groups. None of the tested root canal sealers could eliminate the dye infiltration or microleakage formation. Single cone gutta-percha combined with AH26 sealer exhibited less microleakage than vioseal sealer although there were no statically significant differences between them.

Comparative effects of the conventional, ultrasonic, and laser-activated irrigation on penetration depth of three photosensitizers in the root canal system

ObjectivesThis study compared the effects of conventional, ultrasonic, and laser-activated irrigation (LAI) on penetration depth of three photosensitizers (PSs) in the root canal system. Materials and MethodsIn this in vitro, experimental study, 120 extracted anterior teeth were decoronated such that the remaining root length was standardized at 12 mm. After root canal instrumentation with the ProTaper rotary system and irrigation with 5.25% NaOCl, the roots were assigned to 12 groups for the application of toluidine blue (TB), curcumin, and phycocyanin PSs combined with the LAI using erbium laser with 0.4 mm and 0.6 mm tips, ultrasonic activation, and conventional irrigation. The specimens were sectioned apicocoronally, and the dye penetration depth was quantified in the coronal, middle, and apical thirds under a stereomicroscope at x20 magnification. Data were analyzed by the Kruskal-Wallis and Dunn test (alpha=0.05). ResultsThe effects of irrigation technique, PS type, and their interaction on dye penetration depth were significant at the apical, middle, and coronal thirds (P < 0.0001). TB + LAI with 0.4- and 0.6-mm laser tips showed the highest penetration depth while phycocyanin + LAI or conventional irrigation showed the lowest penetration depth at all areas. Dye penetration depth was the highest in the coronal, and the lowest in the apical third. ConclusionThe LAI technique with erbium laser (0.4- and 0.6-mm tips) enhanced the penetration depth of TB. The tested irrigation techniques had no significant efficacy for enhancement of the penetration depth of curcumin and phycocyanin.

Diffusion in biological media: a comprehensive numerical-analytical study via surface analysis and diffusivities calculation

The study of diffusion in biological materials is crucial for fields like food science, engineering, and pharmaceuticals. Research that combines numerical and analytical methods is needed to better understand diffusive phenomena across various dimensions and under variable boundary conditions within food matrices. This study aims to bridge this gap by examining the diffusion of substances through biological materials analytically and numerically, calculating diffusivity and conducting surface analysis. The research proposes a process for sweetening Bing-type cherries (Prunus avium) using sucrose/xylitol solutions and a staining technique utilising erythrosine and red gardenia at varying concentrations (119, 238 and 357 ppm) and temperatures (40, 50 and 60 °C). Given the fruit's epidermis resistance, the effective diffusivities of skin were inferior to those in flesh. Temperature and concentration synergise in enhancing diffusion coefficients and dye penetration within the food matrix (357 ppm and 60 °C). Red gardenia displayed significant temperature-dependent variation (p = 0.001), whereas erythrosine dye remained stable by temperature changes (p > 0.05). Gardenia's effective diffusivities in cherry flesh and skin, at 357 ppm and 60 °C, 3.89E−08 and 6.61E−09 m2/s, respectively, significantly differed from those obtained at lower temperatures and concentrations. The results highlight the temperature-concentration impacts on mass transfer calculations for food colouring processes and preservation methodologies.

New Evidence of Traditional Japanese Dyeing Techniques: A Spectroscopic Investigation

The Japanese textile tradition is renowned for its intricate designs achieved through a variety of dyeing techniques, including kasuri, shibori, and paste-resist dyeing. These techniques are often combined within a single textile, resulting in exceptionally elaborate creations. Our paper delves into the technical aspects and complexities of these methods, highlighting the dynamic interplay between tradition and innovation in Japanese textile production. Our scientific endeavour focused on some textiles dating between the 19th and 20th centuries and belonging to the Montgomery Collection of Japanese folk art. Employing non-invasive techniques such as visible reflectance spectroscopy and ER-FTIR spectroscopy, we uncovered key insights into the materials and methods utilized in the creation of these textiles. Our analysis revealed a diverse array of pigments and dyes, including plant-derived, inorganic, and synthetic variants. These findings illuminate the cultural syncretism between traditional Japanese practices and the adoption of new materials from the West, underscoring the dynamic nature of textile production in Japan. Furthermore, ER-FTIR spectroscopy elucidated the predominant use of cotton as the primary fibre in the textiles, aligning with historical records of Japan’s role as a major producer of cotton yarn. Analysis of white areas within the textiles revealed evidence of resist-paste dyeing techniques, particularly tsutsugaki and katazome, through the absence of dye penetration and the characteristic appearance of white lines. Confirmation of indigo dyeing techniques (aizome) was achieved through ER-FTIR spectroscopy, providing reliable identification of indigo and Prussian blue in various shades of blue present in the textiles. Additionally, the detection of Western-derived dyeing method (utsushi-yūzen) and free-hand painting (kaki-e), offers insights into the diversity of dyeing practices employed by Japanese artisans. The presence of proteinaceous materials and synthetic dyes observed in some textiles has implications for conservation practices, suggesting the need for tailored approaches to ensure the preservation of these culturally significant artifacts. Overall, these scientific results shed new light on the materials, techniques, and cultural contexts underlying Japanese textile production, advancing our understanding of this rich artistic heritage and informing future research endeavours in textile science and conservation.

A comparative evaluation of marginal fit and microleakage of computer-aided design/computer-aided manufacturing-milled zirconia and prefabricated posterior occlusal veneers: An in vitrostudy.

The aim of this study was to compare the marginal fit of prefabricated occlusal veneers with computer-aided design/computer-aided manufacturing (CAD-CAM)-milled zirconia occlusal veneers in the posterior teeth. Forty extracted human maxillary premolars were divided into two groups of 20 each. Group 1 was prepared to receive prefabricated occlusal veneers, and Group 2 was prepared to receive CAD-CAM-milled zirconia occlusal veneers. Prefabricated samples (Edelweiss) were selected for Group 1, whereas for Group 2, the tooth preparations were scanned, and occlusal veneers were fabricated using Exocad designing software and milling machine. After luting, both the groups were submerged in dye, sectioned, and evaluated for marginal fit and microleakage under a stereomicroscope using the microscope imaging software and its measurement tool. Data collected were subjected to statistical analysis using SPSS 27.0. Intragroup and intergroup comparison was done using the Mann-Whitney U test. The Chi-square test was applied to check the depth of penetration of dye based on percentages. The marginal gap of zirconia occlusal veneers fabricated with CAD-CAM is higher compared to that of prefabricated occlusal veneers. Similarly, the depth of penetration of dye is higher in CAD-CAM-milled zirconia occlusal veneers than prefabricated occlusal veneers. The marginal fit of prefabricated occlusal veneer is better than the marginal fit of zirconia occlusal veneers fabricated with CAD-CAM. Similarly, it can also be concluded that the microleakage of prefabricated occlusal veneer is less compared to the CAD-CAM-milled zirconia occlusal veneers.

Evaluation of the microleakage of new bioactive restorative materials: a comparative in-vitro study.

This in-vitro study aimes to compare the microleakage of different restorative materials namely EQUIA Forte (GC, Japan), Tetric Evoceram (Ivoclar Vivadent, Liechtenstein), and Activa BioACTIVE. A standardized class V cavity has been created on the buccal surface of 40 sound premolar teeth. The cavity had the following measurements: 3 mm buccolingual height, 2 mm axial depth, and 4 mm mesiodistal width. The gingival margin was 1mm below the cementoenamel junction, whereas the coronal margin was on the enamel with cavosurface margins were butt joints all around. Four groups of teeth were restored according to the restorative materials. Under a stereomicroscope, the dye penetration test was used to measure the microleakage after thermocycling of the teeth. Data were compared using Kruskal-Wallis H and Mann-Whitney U tests. Occlusal and gingival margins did not show statistically significant differences among the four groups, but an intragroup analysis revealed a significant difference. The type of restorative material did not appear to have a substantial impact on microleakage, according to the findings of the current investigation.

Evaluation of Immediate and Delayed Placement of Post on Sealing ability of Periapical Area: An Original Research

ABSTRACT Objective: The purpose of this research is to compare the effects of post placement—immediate and delayed—on the periapical area’s capacity to close after root canal therapy (RCT). Techniques: A random split of sixty recently removed human single-rooted teeth was made into two groups: Group A received immediate post placement, whereas Group B received post placement after seven days. Standard guidelines were followed in the preparation of root canals and the placement of posts. A dye penetration method was used to measure microleakage, a measure of sealing capacity. Findings: Compared to the immediate post placement group (0.62 ± 0.08) (P &lt; 0.001), the delayed post placement group had a considerably lower mean microleakage score (0.35 ± 0.06), showing improved sealing capacity. In conclusion, post implantation done later enhances the periapical area’s sealing capacity after RCT. This research underscores the significance of scheduling endodontic treatments to maximize treatment success and improve tooth retention, as well as the possible therapeutic advantages of postponing post implantation.