Pelagic Stage Research Articles

Early-life history of three soft coral species in Okinawa, Japan

ABSTRACT Larval development and subsequent settlement behaviour are important aspects which determine the persistence of sessile benthic organisms on shallow water tropical and subtropical coral reefs. However, only limited studies have been performed on the early-life histories of soft corals in Okinawa, Japan. In this study, we describe the development and settlement pattern of collected spawn materials from two Sclerophytum species and the larvae of a brooding Heteroxenia sp. from Okinawa Island, Japan. Results showed that for the brooding Heteroxenia sp., planulae can metamorphose and fuse at the pelagic stage, and have very low settlement rates on rubble substrates with crustose coralline algae (CCA). On the other hand, the planulae of the two Sclerophytum species have variable survival probability, but both mainly settle on rubble with CCA. These results highlight the differences in larval development and subsequent settlement behaviour and periods among soft coral species. This also serves as the first report describing the larval behaviour and settlement of these species in the subtropical region of Okinawa, Japan.

Ontogenetic transition from aquatic to amphibious life in the mudskipper, Periophthalmus modestus

Vertebrates first emerged from water to land in the Paleozoic. Our understanding about the process has been steadily refined through paleontological studies, although the soft-body traits and behavior of these early animals remain poorly known. Mudskippers, extant amphibious gobies, could give insight into this question. This study reports on the ontogenetic transition from water to land of the mudskipper Periophthalmus modestus under laboratory conditions. After about 30 days after hatching (dah), the fish gradually changed their preference from water to an artificial shore and then to land. After about five days of periodic volitional emersion, the fish became able to propel themselves on land using the pectoral fins and after a further 13 days they began feeding on land. During the transition, the head morphology altered to suit for terrestrial existence. Tissue contents of triiodothyronine (T3) and thyroxine (T4) sharply increased at 30 dah. Forced underwater confinement of larvae at the last pelagic stage (27–29 dah) for 40–42 days resulted in no statistically significant difference in survival or gross morphology of the body and the gills. Growth was slightly stimulated. Our results show that mudskippers emerge on land with little morphological alteration during ontogenesis, much less than the changes observed for amphibians, and that emersion was not indispensable for survival or growth under our laboratory conditions. Further analysis of how and why mudskippers make their way across the water’s edge will shed valuable light on what morphological, behavioral and physiological traits were needed for, and what environmental conditions may have driven the earliest steps of the water-to-land transition in ancient fishes.

A brittle star is born: Ontogeny of luminous capabilities in Amphiura filiformis.

Bioluminescence is the production of visible light by living organisms thanks to a chemical reaction, implying the oxidation of a substrate called luciferin catalyzed by an enzyme, the luciferase. The luminous brittle star Amphiura filiformis depends on coelenterazine (i.e., the most widespread luciferin in marine ecosystems) and a luciferase homologous to the cnidarian Renilla luciferase to produce blue flashes in the arm's spine. Only a few studies have focused on the ontogenic apparitions of bioluminescence in marine organisms. Like most ophiuroids, A. filiformis displays planktonic ophiopluteus larvae for which the ability to produce light was not investigated. This study aims to document the apparition of the luminous capabilities of this species during its ontogenic development, from the egg to settlement. Through biochemical assays, pharmacological stimulation, and Renilla-like luciferase immunohistological detection across different developing stages, we pointed out the emergence of the luminous capabilities after the ophiopluteus larval metamorphosis into a juvenile. In conclusion, we demonstrated that the larval pelagic stage of A. filiformis is not bioluminescent compared to juveniles and adults.

An Introduction to Diopatra, the Amazing Ecosystem Engineering Polychaete.

The annelid genus Diopatra occurs in all major oceans but is best represented in the shallow depths of warmer waters, where it lives in elaborately decorated tubes. This paper provides an introduction to the animals, discussing their history and diversity. We describe and illustrate its morphology and geographic distribution. While they were thought to be predominantly gonochoristic, recent reproductive studies show that several species are protandric simultaneous hermaphrodites. Development is by broadcast spawning with a brief pelagic stage or direct development in the parental tube or egg mass attached to it. Diopatra is a key ecosystem engineer, altering water flow and deposition and increasing the availability of refugia. We also discuss its harvesting as fishing bait, its role as an alien or introduced species, its capacity to regenerate, its therapeutic potential, and its applications as a bioindicator species for climate change, geographic distribution changes, and dispersal.

Whole-genome sequencing reveals fine-scale environment-associated divergence near the range limits of a temperate reef fish.

Environmental variation is increasingly recognized as an important driver of diversity in marine species despite the lack of physical barriers to dispersal and the presence of pelagic stages in many taxa. A robust understanding of the genomic and ecological processes involved in structuring populations is lacking for most marine species, often hindering management and conservation action. Cunner (Tautogolabrus adspersus) is a temperate reef fish with both pelagic early life-history stages and strong site-associated homing as adults; the species is also of interest for use as a cleaner fish in salmonid aquaculture in Atlantic Canada. We aimed to characterize genomic and geographic differentiation of cunner in the Northwest Atlantic. To achieve this, a chromosome-level genome assembly for cunner was produced and used to characterize spatial population structure throughout Atlantic Canada using whole-genome sequencing. The genome assembly spanned 0.72 Gbp and 24 chromosomes; whole-genome sequencing of 803 individuals from 20 locations from Newfoundland to New Jersey identified approximately 11 million genetic variants. Principal component analysis revealed four regional Atlantic Canadian groups. Pairwise FST and selection scans revealed signals of differentiation and selection at discrete genomic regions, including adjacent peaks on chromosome 10 across multiple pairwise comparisons (i.e. FST 0.5-0.75). Redundancy analysis suggested association of environmental variables related to benthic temperature and oxygen range with genomic structure. Results suggest regional scale diversity in this temperate reef fish and can directly inform the collection and translocation of cunner for aquaculture applications and the conservation of wild populations throughout the Northwest Atlantic.

Распространение раковинных брюхоногих моллюсков с длительной и короткой пелагической стадиями в открытых районах Баренцева моря

The duration of the pelagic stage in different species of gastropod molluscs affects their ability to disperse by marine currents. We counted the number of species with long (planktotrophic larva) and short or absent (lecitotrophic larva) pelagic stages in development in shell gastropods from 8 areas of the open part of the Barents Sea. A total of 66 species were identified in the study area, of which 25 were true micromolluscs, and 41 species were represented mainly by juvenile individuals of larger molluscsby larger macrofaunal species. The predominant type of development is lecithotrophic (57 species) and partially pelagic planktotrophic (9 species). According to our data, the shares proportions of species with lecithotrophic and planktotrophic types of development are approximately the same in all investigated areas of the Barents Sea. But in the southern part of the Barents Sea the share of species with a long pelagic stage are sligthlyis slightly higher than in more numerous than in its northern part.

First Record of the Northern Wolffish Anarhichas denticulatus Krøyer, 1845 (Anarhichadidae: Zoarcoidei: Perciformes) in the Siberian Arctic: Further Evidence of Atlantification?

A single specimen of the northern wolffish Anarhichas denticulatus Krøyer, 1845, 393 mm in length, was documented for the first time in the Siberian Arctic (Laptev Sea, Russia). Species identification was confirmed by an integrative taxonomic approach that included examination of external morphology and DNA barcoding using the COI mtDNA gene. This species is widely distributed in the North Atlantic, but records in the Arctic Ocean are limited to the Canadian and US coasts. This record might represent a significant range extension of about 7500 km for the species and may be associated with the eastward transport of a pelagic juvenile specimen from the Northeastern Atlantic to the Laptev Sea by the North Atlantic current, consistent with the hypothesis of Atlantification of the Arctic Ocean. X-ray images of the Laptev Sea specimen and photographs showing ontogenetic variations of species’ coloration are provided for reference. The Laptev Sea specimen had a more elongated shape, longer preorbital distance, and longer pectoral, dorsal, and anal fins, as well as a larger eye and wider caudal fin compared to North Atlantic samples. The size differences are likely associated with conditions experienced as a juvenile during the pelagic stage of the lifecycle.

Fish bioacoustics: Navigating underwater sound.

Fish bioacoustics is about the sounds produced by fish, how fish hear, and what they hear. The focus of this article is on the hypothesis that some late pelagic stage reef fish larvae use the marine soundscape to locate reef settlement habitat. The hypothesis is evaluated by consideration of the nature of reef sound, hearing ability in late-stage larval fish, and direct behavioral evidence for orientation to reef sound.

Pelagic connectivity of European sea bass between spawning and nursery grounds

The sea bass stock around the UK (northern stock) has declined rapidly over the past decade, likely due to a combination of both overfishing and low recruitment. Understanding mechanisms that drive the number of young reaching nursery grounds is important for explaining observed recruitment variability and therefore developing effective management strategies. An individual-based model (IBM) has been developed here to investigate factors affecting sea bass settlement on nursery grounds for the northern stock. Simulations considered seven years with varying environmental conditions and year class strength, and outputs were compared to empirical data from monitoring of nursery areas in the UK and Ireland. The IBM includes hydrodynamics to simulate the drift of eggs and larvae, temperature-dependent growth and mortality, and behavior affecting position in the water column. Comparison between the model and ICES assessment abundance of age class zero fish showed similar temporal variability, but with higher recruitment predicted for 2018, and a lower peak recruitment in 1997. The model reproduced general patterns of interannual variability for the Thames estuary, but performed less well for some other regions. Further investigation is needed to understand differences between modelled supply and observations, as these could be due to uncertainties in reproducing the physical environment (e.g., currents, temperature) or biology (e.g., spawning, behavior, mortality), as well as uncertainty in recruitment derived from stock assessments or data collected from nursery grounds. Nevertheless, the model provides a useful demonstration of connectivity between spawning and nursery grounds, showing spawning regions that are likely to have greatest influence on recruitment in different estuaries, and how these may vary interannually. For example, sources from western Channel and Celtic Sea can supply larvae to many areas, leading to connectivity across the Channel and Celtic Sea, and into the Irish Sea. However, other regions may depend on more local areas. For example, with the Solent estuary dependent on sources within the Channel each year. With the temperature-dependence on growth, and therefore duration of the pelagic stage, results show the potential influence of spawning timing, in relation to ocean temperatures, for connectivity between spawning and nursery grounds.

Blue mussels in western Norway have vanished where in reach of crawling predators

Blue mussels (Mytilus spp.) are declining around the world. In western Norway, they have widely disappeared from rocky shores but still thrive on floating structures. Other refugia are cracks in rocks, exposed sites, and low-salinity habitats. Climate change, pollution, disease, parasites, hybridization, and failed recruitment might not alone be able to create such distribution patterns. We hypothesized that crawling predators that are unable to reach floating structures may drive the present decline in western Norway. A known crawling predator without a pelagic stage and sensitive to low salinity and high wave action is the dogwhelk Nucella lapillus. Tributyltin (TBT) contained in anti-fouling paint rendered this snail sterile, but TBT is now banned, and populations are recovering rapidly. We first surveyed floating structures together with nearby rocky shores for blue mussels and dogwhelks. Blue mussels were present on all surveyed floating docks (65% area covered), but only on 18% of rocky shores (≤5% area covered). Similarly, blue mussels were found on 83% of tree branches suspended in water without bottom contact, but only on 1% when branches touched the seafloor. We then conducted a predator exclusion experiment with caged blue mussels (40-80 mm). In cages, mortality due to factors other than dogwhelks was extremely low (<1%) and confirmed that blue mussels continue to thrive when out of reach of predators. If dogwhelks or other crawling predators such as crabs or sea stars created the observed distribution pattern, then environmentally friendly mariculture with blue mussels growing on rafts and longlines might still have high potential in Norway.

Dispersal of juvenile leatherback turtles from different Caribbean nesting beaches: A model study

The Northwest Atlantic (NWA) leatherback turtle (Dermochelys coriacea) subpopulation was recently classified as endangered. It nests in the Wider Caribbean Region and includes five genetic stocks, all declining, albeit at different rates. The causes of decline are multiple and difficult to identify based on annual nest counts which integrate the effects of multiple stressors over the entire life history. Demographic models, however, show that survival during the juvenile pelagic stage is the main factor modulating population trends, but this life stage remains largely unobserved. This paper presents a suite of numerical simulations where juveniles from the five NWA stocks disperse under the combined effects of ocean currents and habitat-driven swimming movements. Simulations reveal when and where NWA juveniles likely disperse and, thus, the environmental conditions and anthropogenic threats they may encounter. Simulated individuals initially disperse following either the “Caribbean route,” inside the Caribbean Sea and the Gulf of Mexico (GoM), or the “Atlantic route” east of the Antilles Islands Arc. The percentage of individuals following one or the other route varies markedly with the stock of origin. Late dispersal in the eastern Atlantic Ocean and the Mediterranean Sea is similar in all stocks. Juveniles following the Caribbean route are rapidly entrained northwards by the Gulf Stream and incur a high risk of cold-induced mortality. This mostly affects the Florida stock and the Western Caribbean (WCA) stock nesting in Costa Rica, Panama, and Colombia. The Atlantic route is less lethal as individuals progress more slowly toward higher latitudes. Simulations also show that the percentage of WCA juveniles visiting the GoM is larger than for any other stock. The learned migration goal (LMG) hypothesis, which posits that adult sea turtles tend to exploit foraging areas previously identified at the juvenile stage, may thus explain why WCA adults are overrepresented in the GoM. Finally, our results suggest that the recently observed increase in the percentage of WCA adults migrating into the GoM could be linked to bycatch reduction measures implemented in 2003–2004, combined with an increase in the frequency of Loop Current intrusion and eddy-shedding events that started around the year 2000.

Shell-bearing Gastropoda from the methane seeps and hydrothermal vents of the Bering Sea: A preliminary description

The first description of the fauna of shell-bearing gastropod from chemosynthesis-based communities of the Bering Sea is given. The work includes materials collected during the cruises of the RV Akademik M.A. Lavrentyev in 2016 and 2018 in two types of the reducing biotopes: hydrothermal fields on the underwater Piip Volcano and the methane seeps on the Koryak slope of the Bering Sea. The Piip Volcano (∼ 368–495 m) is the northernmost (55°22’ – 55°24’ N) hydrothermal region in the Pacific. Methane seep fields of the Koryak slope found between 400 and 700 m depth are the northernmost chemosynthesis-based habitats known to date in the Pacific (60°49–61°10). In total, 27 recognizible taxonomic units (RTUs) of shell-bearing gastropods were identified from both areas, at least six of them presumably belong to species new to science. Also, five species are new to the fauna of the Bering Sea and adjacent areas of the Pacific Ocean. Only nine RTUs were found on the Piip Volcano, among which there was one species, Provanna sp. nov., belonging to the family known only from chemosynthesis-based communities and another species, Parvaplustrimwareni, was potentially obligate for chemosynthesis-based communities. Nineteen RTUs have been identified in the methane seeps of the Koryak slope, however no taxa specific for chemosynthesis-based communities had been recorded. Gastropods have less aggregated population structure on the Koryak slope than on the Piip Volcano, where four times as many specimens have been collected. In general, the pattern of distribution of taxonomic and functional groups in the area of methane seeps of the Koryak slope is presumably the same as in the background communities while in the hydrothermal zone of the Piip Volcano it is similar to other types of extreme communities. Majority of gastropods from both regions feed on detritus or invertebrates. Most RTUs have a lecitothropic protoconch, which corresponds to a short period or absence of pelagic stage in their development. This makes the species associations of gastropods vulnerable.

Ocean temperature and density dependence as key drivers of the population dynamics of an intertidal crab at the Brazilian oceanic islands

AbstractUnderstanding how climatic and density‐dependent processes affect demography is crucial for predicting population responses to climate change. For marine invertebrates with complex life cycle such as decapod crustaceans, increasing temperatures might affect survival and development of early pelagic stages, whereas high density can increase competition and thus reduce growth and fecundity of older life stages. In this study, we investigate the effects of warm ocean events, body size and density on the population dynamics of the intertidal Sally lightfoot crab (Grapsus grapsus) at the Brazilian oceanic islands. Firstly, we assessed the trends of marine heatwaves (MHW) and positive temperature anomalies (ΔSST+) at the equatorial St Peter and St Paul (SPSP) Archipelago and Rocas Atoll and the subtropical Trindade Island. We then jointly analyzed short‐term count, capture‐recapture and fecundity data, and long‐term population monitoring data (2003–2019) using an integrated population model. Warm ocean events have become more frequent and intense only at the equatorial islands. Increasing MHW frequency positively influenced recruitment in the high‐density SPSP population, while MHW intensity and ΔSST+ frequency had negative impacts. Conversely, no climatic effects were observed for the low‐density Rocas population, which has the largest crabs. Despite a lack of warming in Trindade, this subtropical population with intermediate density and body size was negatively affected by ΔSST+. Our findings revealed population‐specific responses to climate change when accounting for local life history and ecology. Thus, environmental and density‐dependent effects should be broadly considered in future conservation studies regarding ocean warming impacts on marine invertebrate populations.

Staying in place and moving in space: Contrasting larval thermal sensitivity explains distributional changes of sympatric sea urchin species to habitat warming.

For marine ectotherms, larval success, planktonic larval duration and dispersal trajectories are strongly influenced by temperature, and therefore, ocean warming and heatwaves have profound impacts on these sensitive stages. Warming, through increased poleward flow in regions with western boundary currents, such as the East Australia Current (EAC), provides opportunities for range extension as propagules track preferred conditions. Two sea urchin species, Centrostephanus rodgersii and Heliocidaris tuberculata, sympatric in the EAC warming hotspot, exhibit contrasting responses to warming. Over half a century, C. rodgersii has undergone marked poleward range extension, but the range of H. tuberculata has not changed. We constructed thermal performance curves (TPC) to determine if contrasting developmental thermal tolerance can explain this difference. The temperatures tested encompassed present-day distribution and forecast ocean warming/heatwave conditions. The broad and narrow thermal optimum (Topt) ranges for C. rodgersii and H. tuberculata larvae (7.2 and 4.7°C range, respectively) matched their realized (adult distribution) thermal niches. The cool and warm temperatures for 50% development to the feeding larva approximated temperatures at adult poleward range limits. Larval cool tolerances with respect to mean local temperature differed, 6.0 and 3.8°C respectively. Larval warm tolerances were similar for both species as are the adult warm range edges. The larvae of both species would be sensitive to heatwaves. Centrostephanus rodgersii has stayed in place and shifted in space, likely due to its broad cold-warm larval thermal tolerance and large thermal safety margins. Phenotypic plasticity of the planktonic stage of C. rodgersii facilitated its range extension. In contrast, larval cold intolerance of H. tuberculata explains its restricted range and will delay poleward extension as the region warms. In a warming ocean, we show that intrinsic thermal biology traits of the pelagic stage provide an integrative tool to explain species-specific variation in range shift patterns.

Incorporating environmental variability in a spatially-explicit individual-based model of European sea bass✰

The northern stock of European sea bass (Dicentrarchus labrax) is a large, high value, slow growing and late maturing fish that is an important target species for both commercial and recreational fisheries. Around the UK, scientific assessments have shown a rapid eight-year decline in spawning stock biomass since 2010 attributed to poor recruitment; this was likely driven by environmental factors and high fishing mortality. Management of the stock is informed by scientific assessments in which a population model is fitted to the available data and used to forecast the possible consequences of various catch options. However, the model currently used cannot represent the spatial distribution of the stock or any effects of environmental variability. One approach that may be used to represent the effects of spatial and temporal variation in environmental drivers is with Individual based models (IBMs). In IBMs populations are represented by their constituent individuals that interact with their environment and each other. The mechanistic nature of IBMs is often advantageous as a management tool for complex systems including fisheries. Here we add to an existing IBM to produce a spatio-temporally explicit IBM of the northern stock of sea bass in which individual fish respond to local food supply and sea surface temperature. All life stages (i.e., pelagic stages, juvenile and mature fish) are modelled and individual fish have their own realistic energy budgets driven by observed dynamic maps of phytoplankton density and sea surface temperature. The model is calibrated using Approximate Bayesian Computation (ABC). After calibration by ABC the model gives good fits to key population parameters including spawning stock biomass. The model provides a mechanistic link between observed local food supplies and sea surface temperatures and overall population dynamics. Plots of spatial biomass distribution show how the model uses the energy budget to predict spatial and temporal change in sea bass biomass distribution in response to environmental variability. Our results indicate that the IBM is a promising approach that could be used to support stock assessment with scope for testing a range of spatially and temporally explicit management scenarios in addition to testing stock responses to novel environmental change.

Avrupa Istakozu (Hommarus gammarus) Larvalarının Düşük Yoğunluk Koşulları Altında Pseudo-Yeşil Su ve Temiz Suda Büyümesi ve Hayatta Kalması

The European lobster, Hommarus gammarus, is a commercially important species in Europe. Despite successful stock enhancement programs during the last two decades, culture methods of H. gammarus are still in progress. In this study, the effects of pseudo-green water technique and clear water technique with lower stocking densities on the growth and survival of H. gammarus larvae during stages I-IV were investigated. All larvae were reared in 800 L cylindro-conical tanks with a stocking density of 1.25 larvae/L at a temperature of 17.1±1 oC and a salinity of 32±1 ppt. Lobster larvae were fed with enriched Artemia at a density of 3-5 naupli/ml for a period of 10 (09:30 am- 19:30 pm) hours. Daily additions of concentrated algae increased turbidity and reduced visibility in tanks compared to clear water conditions. Results showed that growth of lobster larvae were not significantly different among treatments during planktonic stages I-IV (p>0.05). Overall mean survival rate was only 3% with no significant differences between treatments. Increased turbidity and lower stocking density did not improve survival rates. Further studies are required to develop methods that will promote higher survival rates during the pelagic stages of lobster larvae.

Fishers' Ecological Knowledge and Stable Isotope Analysis Reveal Mangrove Estuaries as Key Developmental Habitats for Critically Endangered Sea Turtles

Successful conservation of endangered, migratory species requires an understanding of habitat use throughout life stages. When dedicated scientific studies are difficult to conduct, local expert knowledge can provide crucial baseline data to guide study design and aid data interpretation. In 2008, fishers in El Salvador demonstrated that eastern Pacific hawksbill sea turtles (Eretmochelys imbricata)—a population conservation biologists considered virtually extirpated—use mangrove estuaries as nesting habitat rather than open-coast beaches used by hawksbills in other regions. We confirmed and amplified this observation by using fishers' ecological knowledge to guide biological sampling for stable isotope analysis to assess if eastern Pacific hawskbills use mangrove-dominated estuaries as developmental habitats. We found that immature hawksbills experience a pelagic stage and then recruit to estuaries at ~37 cm curved carapace length, where they increase reliance on estuarine resources until they approach adult sizes. This life history strategy makes them especially vulnerable to in-water nearshore threats, and necessitates targeted expansion of conservation efforts throughout the eastern Pacific. Our analysis also provides a model for integrating traditional scientific approaches with local knowledge—a model that could yield crucial advances in other understudied regions.

Habitat Shift for Plankton: The Living Side of Benthic-Pelagic Coupling in the Mar Piccolo of Taranto (Southern Italy, Ionian Sea)

Resting stages represent the answer for species to the variability of environmental conditions. In confined marine habitats, variability of conditions is high, and bottoms host plankton resting stages in the so-called “marine cyst banks”. The Mar Piccolo of Taranto was chosen as a pilot site in which to investigate how marine cyst banks and plankton affect each other in the living part of the benthic–pelagic coupling. The attempt was based on the use of multiple devices for integrated sampling of benthic and pelagic stages and allowed us to identify 207 taxa/categories in the whole system (127 as active forms, 91 as resting stages). The sediments added 80 taxa to the plankton list obtained only from the water column, thus confirming the importance of this kind of approach in perceiving the actual diversity of the studied site. The sediment cyst bank involved 0.15–1.00% of its content in daily benthic-pelagic exchanges, in terms of cyst germination and import, respectively. In addition, the cyst production, which was higher than the cyst germination, is responsible for the existence of a permanent biological reservoir in the sediments. The benthic-pelagic coupling, however, was completely depicted in the present investigation only for seven taxa. This result is due to the still scant knowledge of the life cycles and life histories of single species. Apart from the identification difficulties that still have to be clarified (which cysts belong to which species), the cycle presence/absence is also characterized by the diversification of strategies adopted by each species. The observation of plankton dynamics from the benthos point of view was useful and informative, unveiling a huge assemblage of resting forms in the sediments only minimally affected by cyst import/export, because it is more devoted to a storing role over long periods. Consequently, the continuation of life cycle studies appears necessary to understand the diversity of strategies adopted by the majority of plankton species.

Separate Feeding Between the Pelagic Stage of the Squat Lobster Munida gregaria and the Larger Sized Zooplankton Crustacean Groups in the Beagle Channel as Revealed by Stable Isotopes

In southern Patagonia, the Beagle Channel shows very low production during winter but simultaneously sustains very dense aggregations of the pelagic stage of squat lobster (Munida gregaria), a benthic decapod whose pelagic juveniles have the largest body size within the chitinous pelagic community. To assess the coexistence of the mesozooplankton community and the pelagic M. gregaria stage under the harsh feeding winter conditions, we conducted a research cruise at two locations connected to the Beagle Channel, Yendegaia Bay (land terminating-glacier) and Pia Fjord (marine-terminating glacier). Our results showed that the zooplankton communities were similar in these two fjords, that a single pelagic group dominated in terms of biomass (pelagic Munida gregaria), and that differences in vertical distribution existed between most of the principal crustacean zooplankton and pelagic M. gregaria. All groups showed consumption of terrestrially derived organic matter, as revealed by their δ13C values. However, the isotopic composition, trophic positions (TP), and isotopic niche areas of the groups separated pelagic M. gregaria, presenting some of the lowest δ15N and the highest δ13C values, and the narrowest isotopic niche width. Pelagic M. gregaria was dominated by a single body size class along the 0–100 m water column, with no diel changes in vertical distribution, remained mostly in the upper layers (0–50 m), and benefited from the slightly higher phytoplankton concentrations at shallower depths as revealed by their higher δ13C values and low trophic position. In contrast, the other groups, including zoea M. gregaria stages, developed changes in distribution between day and night or remained deeper in the water column. These groups showed higher δ15N values, higher TP, and lower δ13C values, most of which probably fed on a nanoheterotrophs and terrestrial particulate organic matter mixture at deeper layers. Thus, the different vertical distributions, different trophic level food sources, and slightly different organic carbon sources apparently reduced any potential competence for food resources and form part of the feeding strategy that may facilitate the coexistence of the different large pelagic crustaceans under harsh feeding winter conditions in this high latitude austral region.

Copper promoting oyster larval growth and settlement: Molecular insights from RNA-seq

As a cofactor of key enzymes, Cu is required in living organisms, although Cu levels in the natural environment are typically low. In this study, the promotion of growth and settlement on the larvae of oyster Crassostrea angulata was observed at an environmentally relevant concentration (10 μg/L Cu). Interestingly, Cu accumulation in the soft tissue of oyster larvae increased during the larval development and exhibited a sharp increase at the late pelagic stage. With the help of RNA-seq, we constructed a high-quality transcriptional database of the oyster C. angulata larvae (24,257 genes with an average length of 1594 bp) via de novo assembly, which provided the basic molecular changes during the larval development. Network analysis of five early developmental stages and differential expression under Cu exposure were integrated to examine the roles of Cu in oyster larvae. Our molecular analysis demonstrated that both ion channels and organic transporters contributed to Cu internalization from the external environment, including zinc transporters and amino acid transporters. The followed distribution of Cu across cells was achieved by ATP7A, the circulatory system, and the Cu transporters (CTRs). Cu exposure enhanced the ribosome and the calcium binding proteins with a higher rate of translation and shell formation, giving rise to faster growth of oyster larvae. Furthermore, Cu facilitated the settling process by upregulating the chitin binding genes and then promoting the formation of the proteinaceous matrix between larvae and substrate. Our study presents the molecular basis for Cu promotion (i.e., hormesis) effects on oyster larval growth and settlement.