Pelagic Fish Research Articles

The gills and skin microbiota of five pelagic fish species from the Atlantic Ocean.

The gills and skin microbiota and microbiome of wild fish remain far more under-investigated compared to that of farmed fish species, despite that these animal-microbe interactions hold the same ecophysiological roles in both cases. In this study, the gills and skin bacterial microbiota profiles and their presumptive bacterial metabolisms were investigated in five open-sea fishes: bullet tuna (Auxis sp.), common dolphinfish (Coryphaena hippurus), Atlantic little tunny (Euthynnus alletteratus), Atlantic bonito (Sarda sarda) and Atlantic white marlin (Kajikia albida). Gills and skin tissues were collected from two to three individuals per species, from specimens caught by recreational trolling during summer of 2019, and their bacterial 16S rRNA gene diversity was analysed by high-throughput sequencing. The gills bacterial communities among the five species were clearly different but not the skin bacterial microbiota. The dominant operational taxonomic units belonged to the Moraxellaceae, Pseudomonadaceae, Rhodobacteraceae, Staphylococcaceae and Vibrionaceae families. Despite the differences in taxonomic composition, the presumptive bacterialmetabolisms between the gills and skin of the five fishes investigated here were ≥ 94% similarand were dominated by basic metabolism, most likely reflecting the continuous exposure of these tissues in the surrounding seawater.

Phylogenetic relationships analysis of the family Scombridae (Actinopterygii, Scombriformes)

Scombridae is a family of pelagic marine fishes that comprises 16 genera and 51 species. This family has been of significant commercial importance throughout history; however, the phylogenetic relationships within the Scombridae have been disputed due to the unclear taxonomic boundaries of the suborder Scombroidei, which includes six families, including Scombridae. Despite this, only a limited number of studies have been conducted on the Scombridae. In our study, eight species covering five genera of the Scombridae were selected, and one nuclear (ITS) and three mitochondrial DNA markers (CO1, Cytb, and D-loop) were used to amplify gene fragments. Additionally, we included homologous sequences from other Scombridae fishes obtained from GenBank. Our analysis constructed phylogenetic relationships of 48 Scombridae species in 14 genera. The results demonstrated that the three phylogenetic trees (NJ, ML, and BI) exhibited similar topologies, containing three major clades. One major clade indicated that Grammatorcynus bilineatus and Grammatorcynus bicarinatus did not cluster with other species in the Scombridae; another clade comprised the genera Scomber and Rastrelliger; the third clade consists of the remaining Scombridae species. Notably, the study showed that Gasterochismatinae and Scombrinae were not sister groups; Allothunnus (tribe Thunnini) and Cybiosarda (tribe Sardini) clustered into a clade, suggesting that Sardini and Thunnini were non-monophyletic. Overall, this research enhances the understanding of phylogenetic relationships within the Scombridae and provides basic information to aid further research.

Improved YOLOv8-Pose Algorithm for Albacore Tuna (Thunnus alalunga) Fork Length Extraction and Weight Estimation

Aiming at the problems of large statistical error and the poor real-time performance of catch weight in the ocean fishing tuna industry, an algorithm based on improved YOLOv8-Pose for albacore tuna (Thunnus alalunga) fork length extraction and weight estimation is proposed, with reference to the human body’s pose estimation algorithm. Firstly, a lightweight module constructed using a heavy parameterization technique is used to replace the backbone network, and secondly, a weighted bidirectional feature pyramid network BIFPN is utilized. Finally, the upper and lower jaw and tail feature points of the albacore tuna (Thunnus alalunga) were extracted using the key point detection algorithm, and the weight of the albacore tuna (Thunnus alalunga) was estimated based on the fitted relationship between fork length and weight. The experimental results show that the improved YOLOv8-Pose algorithm reduces the number of model parameters by 13.63% and the number of floating-point operations by 14.03% compared with the baseline model without decreasing the accuracy of the target detection and key point detection and improves the model inference speed by 374%. At the same time, it reduces the drift of the key point detection, and the error of the comparison with the actual albacore tuna (Thunnus alalunga) body weight is not more than 10%. The improved key point detection algorithm has high detection accuracy and inference speed, which provides accurate yield data for pelagic fishing and is expected to solve the existing statistical problems and improve the accuracy and real-time performance of data in the fishing industry.

Species-specific and habitat-dependent bioaccumulation of halogenated flame retardants in marine organisms from estuary to coastal seas

Halogenated flame retardants (HFRs) have attracted global attention owing to their adverse effects on ecosystems and humans. The Shandong Peninsula is the largest manufacturing base for HFRs in East Asia, yet its impacts on marine ecosystems are unclear. Seventeen HFRs were analyzed in organisms captured from the Xiaoqing River estuary, Bohai Sea (BS), Yellow Sea and Northern East China Sea to investigate the distribution and bioaccumulation of HFRs on a broad scale. The results showed a downward trend in ΣHFR concentrations from the estuary (37.7 ng/g lw on average) to Laizhou Bay (192 ng/g lw) and to coastal seas (3.13 ng/g lw). The concentrations of ΣHFRs were significantly higher in demersal fish (0.71–198 ng/g lw) and benthic invertebrates (0.81–3340 ng/g lw) than in pelagic fish (0.30–27.6 ng/g lw), reflecting a habitat dependence. The concentrations of higher-brominated homologs were greater in benthic invertebrates, whereas a greater level of lower-brominated PBDE congeners was observed in fish, suggesting different profiles between species. Furthermore, the analogue composition of HFRs in fish was similar to that in the dissolved phase of seawater, whereas the HFR pattern in benthic invertebrates was consistent with the profile in sediment. The concentrations of HFRs in organisms vary widely depending on emissions from anthropogenic activities, whereas bioaccumulation patterns are strongly influenced by species and habitat.

Combining mesocosms with models reveals effects of global warming and ocean acidification on a temperate marine ecosystem.

Ocean warming and species exploitation have already caused large-scale reorganization of biological communities across the world. Accurate projections of future biodiversity change require a comprehensive understanding of how entire communities respond to global change. We combined a time-dynamic integrated food web modeling approach (Ecosim) with previous data from community-level mesocosm experiments to determine the independent and combined effects of ocean warming, ocean acidification and fisheries exploitation on a well-managed temperate coastal ecosystem. The mesocosm parameters enabled important physiological and behavioral responses to climate stressors to be projected for trophic levels ranging from primary producers to top predators, including sharks. Through model simulations, we show that under sustainable rates of fisheries exploitation, near-future warming or ocean acidification in isolation could benefit species biomass at higher trophic levels (e.g., mammals, birds, and demersal finfish) in their current climate ranges, with the exception of small pelagic fishes. However, under warming and acidification combined, biomass increases at higher trophic levels will be lower or absent, while in the longer term reduced productivity of prey species is unlikely to support the increased biomass at the top of the food web. We also show that increases in exploitation will suppress any positive effects of human-driven climate change, causing individual species biomass to decrease at higher trophic levels. Nevertheless, total future potential biomass of some fisheries species in temperate areas might remain high, particularly under acidification, because unharvested opportunistic species will likely benefit from decreased competition and show an increase in biomass. Ecological indicators of species composition such as the Shannon diversity index decline under all climate change scenarios, suggesting a trade-off between biomass gain and functional diversity. By coupling parameters from multilevel mesocosm food web experiments with dynamic food web models, we were able to simulate the generative mechanisms that drive complex responses of temperate marine ecosystems to global change. This approach, which blends theory with experimental data, provides new prospects for forecasting climate-driven biodiversity change and its effects on ecosystem processes.

Trophic interactions between common minke whales (Balaenoptera acutorostrata) and their prey during summer in the northern Barents Sea

Global warming is causing rapid change in marine food webs, particularly at northern latitudes where temperatures are increasing most rapidly. In this study, the diet of common minke whales Balaenoptera acutorostrata was assessed both in terms of short-term (morphological analyses of digestive tract contents) and longer-term (tissue chemical markers: fatty acids and stable isotopes) prey use in the northern Barents Sea to see if they are prey shifting. Samples (blubber cores, muscle, and stomach contents) were obtained from 158 common minke whales taken during Norwegian commercial whaling operations during summer over the period 2016–2020. Two prey items, capelin Mallotus villosus and krill (primarily Thysanoessa sp.), dominated the stomach contents in the entire period of investigation, which included sampling both in June and in August, similar to findings from earlier studies. A few gadoids were also observed in the whale stomachs. Lower blubber fatty acid (FA) contents in 2016/2017 as compared with 2018/2019 were observed. This is most likely explained by differences in sampling time (June in 2016/2017 vs August in 2018/2019, i.e., after a longer feeding period during the summer in the latter case). This explanation also fits with the fact that FA profiles of the 2018/2019 whales were more similar to the FA profiles of the potential prey, presumably reflecting the two months longer assimilation time for these whales. Multidimensional mixing models based on carbon and nitrogen isotope composition of the most likely prey groups suggested that the whales ate mostly krill in four of the five sampling years. In 2018 there were indications of a higher proportion of gadoid fish, showing some dietary flexibility. The trophic level of the whales’ feeding, as interpreted from the nitrogen isotope values, was positively correlated with blubber thickness suggesting that fish-eaters tended to assimilate more energy than whales that focused more exclusively on lower trophic prey. The variation suggested by different dietary analyses methods − stomach contents, fatty acids, and stable isotopes – most likely reflects different turnover times, with muscle stable isotopes likely representing several months of dietary integration, while lipid stores are more dynamic and may represent weeks, and stomach contents represent feeding events during the last few hours. The change in diet of minke whales from small pelagic fishes (in the past) to a greater quantity of krill and demersal fish (seen in this study) suggests that the whales are responding to the ongoing borealization of the Barents Sea ecosystem.

Southernmost record of Coryphaena hippurus Linnaeus, 1758 (Coryphaenidae) in the southwest Atlantic Ocean, off Mar del Plata, Argentina

This paper reports the presence of the common dolphinfish, Coryphaena hippurus, in Argentinian waters for the first time, with a specimen collected by experienced sport fishermen near Mar del Plata (38°S). This finding also represents the first record of a species from the Coryphaenidae family in Argentinian waters. The collection of these specimens coincided with sightings of other rarely-seen pelagic and oceanic fish species during the same period, providing further evidence supporting the influx of subtropical fish species from warmer Brazilian waters. These observations highlight the need for continued monitoring of fish populations and their distribution and the ecological dynamics within the region.

Assessing the potential economic effects of mesopelagic fisheries as a novel source of fishmeal

AbstractThe continuous growth of the aquaculture industry implies increased demand for efficient sources of aquafeed, such as fishmeal. Pelagic fish are a desirable source of fishmeal due to their high nutritional content. Nevertheless, several pelagic stocks that have been exploited extensively for fishmeal production face ecological limits due to commercial exploitation, and the aquaculture industry is now seeking novel, efficient, and sustainable sources of aquafeed. The mesopelagic zone, an ecosystem with many scientific uncertainties, is being considered as a potential source for fishmeal, largely owing to the abundance of mesopelagic fish and their robust nutritional profile. However, both the ecological and economic viability of commercial exploitation of mesopelagic fish are not yet well understood. To understand the conditions that would make such an endeavor economically viable in the context of global fishmeal production systems, we use a bioeconomic model that assesses the economic consequences of including mesopelagic fish as a fishmeal source. Through simulations, we assess the economic implications of this hypothetical mesopelagic fishery on major pelagic fishmeal production systems. The mesopelagic fishery can be economically profitable for harvesters, and its addition to global fishmeal production reduces fishmeal market price, thus making it more accessible to aquaculture farmers and less profitable for pelagic fishers. While this may reduce fishing pressure on pelagic forage‐fish stocks, the implications of commercial exploitation of mesopelagic on key ecosystem services remain a concern.

Mass capture fishing in the Marquesas Islands

ABSTRACTMass capture of small fishes with a variety of nets, traps, and weirs was widely practiced and economically important across East Polynesia at western contact. Archaeological research, however, has suggested these technologies were less important during the early settlement period and gained prominence over time. Several explanations have been proposed, including resource depression, changes in marine environments, and/or social and economic reorientations. In the Marquesas Islands, pelagic and offshore fishes were historically well represented in early assemblages relative to most Polynesian islands. Here we report on fishbone assemblages from Nuku Hiva Island that were recovered with fine mesh screens, identified using a wide range of skeletal elements, and analysed with morphometric methods. The Hakaea Beach results demonstrate that mass capture of small fishes was especially important at this locality and sustained over three early, successive occupations. These patterns may reflect the nature of the local fisheries, preferential use of high‐return capture strategies in this reef‐limited setting, and/or purposeful avoidance of ciguatera‐prone fishes and a preference for less vulnerable fishes. Overall, our findings highlight geographic variation in early Marquesan fisheries and provide archaeological evidence that mass capture technologies had an important place in the maritime toolkits of the earliest East Polynesian fishers.

Atlantic mackerel population structure does not support genetically distinct spawning components

Background The Atlantic mackerel, Scomber scombrus (Linnaeus, 1758) is a commercially valuable migratory pelagic fish inhabiting the northern Atlantic Ocean and the Mediterranean Sea. Given its highly migratory behaviour for feeding and spawning, several studies have been conducted to assess differentiation among spawning components to better define management units, as well as to investigate possible adaptations to comprehend and predict recent range expansion northwards. Methods Here, a high-quality genome of S. scombrus was sequenced and annotated, as an increasing number of population genetic studies have proven the relevance of reference genomes to investigate genomic markers/regions potentially linked to differences at finer scale. Such reference genome was used to map Restriction-site-associated sequencing (RAD-seq) reads for SNP discovery and genotyping in more than 500 samples distributed along the species range. The resulting genotyping tables have been used to perform connectivity and adaptation analyses. Results The assembly of the reference genome for S. scombrus resulted in a high-quality genome of 741 Mb. Our population genetic results show that the Atlantic mackerel consist of three previously known genetically isolated units (Northwest Atlantic, Northeast Atlantic, Mediterranean), and provide no evidence for genetically distinct spawning components within the Northwest or Northeast Atlantic. Conclusions Therefore, our findings resolved previous uncertainties by confirming the absence of genetically isolated spawning components in each side of the northern Atlantic, thus rejecting homing behaviour and the need to redefine management boundaries in this species. In addition, no further genetic signs of ongoing adaptation were detected in this species.

Exploring tradeoffs in southeast United States marine fisheries management using management strategy evaluation

Recreational fishing is the fastest growing sector in industrialized nations and can have substantial impacts on marine fish populations and ecosystems. Commercial objectives are typically characterized by maximizing yield and fishery stability whereas recreational anglers generally prefer sustained access to fishing and the availability of larger fish. Achieving these objectives while balancing tradeoffs between recreational and commercial fishing is essential to effective recreational and mixed-use fisheries management. Balancing multiple sector objectives is of particular concern in the southeast United States (US), where recreational fishing is the dominant source of mortality for marine fish stocks. We developed and applied a size-structured management strategy evaluation (MSE) tool, individually, to two stocks in southeast US Atlantic waters, black sea bass (Centropristis striata), a sedentary reef fish in overfished condition, and cobia (Rachycentron canadum), a migratory coastal pelagic fish, to evaluate the performance and tradeoffs of mixed management procedures against a variety of objectives. Several recreational and commercial objectives were aligned: fishers sought to conserve the stock and maximize catch, which simplified the evaluation of tradeoffs. We tested management procedures over alternate states of recruitment for black sea bass, and natural mortality for cobia. Management procedures that allowed harvest for black sea bass resulted in or risked an overfished status if the current weak recruitment regime continues, but could rebuild with no fishing regardless of recruitment state. Although results were sensitive to uncertainty in recruitment, no management procedures could achieve historic landings for either sector, and only management procedures that reduced effort consistently resulted in a reduced number of dead discards. No management procedures resulted in an overfished status for cobia. Management procedures for cobia generally achieved objectives and were robust to uncertainty in natural mortality. In both case studies, tradeoffs occurred between maximizing catch and season length and maintaining a stronger size structure in the population. This study resulted in a flexible MSE tool with strong potential utility for scoping management procedures that explicitly consider recreational fishing objectives.

Analysis of mesoscale frontal zones of the Norwegian Sea based on satellite observations and reanalysis data in May 2011–2020

   The aim of this study is to compare the horizontal temperature gradients calculated based on satellite observations and reanalysis data in the area of mesoscale frontal zones’ surface manifestations, both for the entire Norwegian Sea and during the onset of pelagic fish spawning migrations in May from 2011 to 2020.   Using monthly average temperature data from MODIS/Aqua, GHRSST OSTIA, and CMEMS GLORYS12v1, the fields of monthly and decade-long horizontal gradients on the surface of the Norwegian Sea were derived. A comparison was made between the decade-long temperature gradient estimates and their mean climatic values. The majority of the data show surface manifestations of the main frontal zones in the Norwegian Sea. Validation of temperature fields in the area of the Iceland-Faroe Frontal Zone was conducted based on unique in situ observations. It was demonstrated that for the analysis of the frontal zones in the Norwegian Sea, using the temperature fields of GHRSST OSTIA is most preferable. The obtained physico-geographical characteristics of mesoscale frontal zones could be used to assess their relationship with pelagic fish migrations in the Norwegian Sea.

Enjoy the darkness: Forsake partially nocturnal sets provides a good opportunity to improve profits and sustainability in the southern Brazil longline fishery

Pelagic longline fishing globally raises concerns for adverse interactions with non-target species, prompting the need for sustainable practices and effective mitigation. Adherence to bycatch measures varies globally, driving efforts to find cost-effective alternatives sustaining target species catch while minimizing bycatch. Our study, based in a previous research in southwestern Atlantic Ocean, confirms that fully nocturnal sets (FNS) are more effective in capturing target species (e.g. swordfish, tunas) and reducing bycatch of mako shark and loggerhead turtle compared to partially nocturnal sets (PNS). Extending the analysis to warm and cold seasons, FNS consistently outperforms PNS. In terms of profitability, trips with more FNS are generally more profitable than those with more PNS. Adopting only FNS in pelagic longline fishing seems to be viable, efficient in capturing target species, minimize bycatch, and potentially increasing profits—a promising solution for sustainable and economically viable pelagic longline fishing in southern Brazil.

Whole-Genome Sequencing Analyses Reveal the Evolution Mechanisms of Typical Biological Features of Decapterus maruadsi.

Decapterus maruadsi is a typical representative of small pelagic fish characterized by fast growth rate, small body size, and high fecundity. It is a high-quality marine commercial fish with high nutritional value. However, the underlying genetics and genomics research focused on D. maruadsi is not comprehensive. Herein, a high-quality chromosome-level genome of a male D. maruadsi was assembled. The assembled genome length was 716.13 Mb with contig N50 of 19.70 Mb. Notably, we successfully anchored 95.73% contig sequences into 23 chromosomes with a total length of 685.54 Mb and a scaffold N50 of 30.77 Mb. A total of 22,716 protein-coding genes, 274.90 Mb repeat sequences, and 10,060 ncRNAs were predicted, among which 22,037 (97%) genes were successfully functionally annotated. The comparative genome analysis identified 459 unique, 73 expanded, and 52 contracted gene families. Moreover, 2804 genes were identified as candidates for positive selection, of which some that were related to the growth and development of bone, muscle, cardioid, and ovaries, such as some members of the TGF-β superfamily, were likely involved in the evolution of typical biological features in D. maruadsi. The study provides an accurate and complete chromosome-level reference genome for further genetic conservation, genomic-assisted breeding, and adaptive evolution research for D. maruadsi.

Analysis of two coastal pelagic fish species (Sardinella aurita and Scomber colias) for early detection of the impact of anthropogenic metals released into the marine environment

In this study, the concentrations of several metals (Al, Cd,Cr, Cu, Fe, Pb, Zn) were analyzed in two species of fish (S. aurita and S. colias) in different years, including the period of the COVID-19 pandemic. The highest values of metals such as Al were found in the years 2019 8.661±0.434 mg/kg in S. colias and 6.487±1.485 mg/kg in S. aurita, and the year with the lowest concentration was 2020; 7.5±0.146 mg/kg in S. colias and 4.405±0.823 mg/kg in S. aurita, this pattern of concentrations occurs similarly for the other metals studied. Significant differences were found in the concentrations of these metals in both types of fish over the years. In general, metal concentrations were higher in 2019 and lower in 2020, coinciding with the period of reduced human activity during the pandemic. During the COVID-19 pandemic, reduced human activity led to a decrease in metal pollution in the marine environment, which had a positive impact on fish. However, it was noted that these effects may be temporary and may reverse when normal economic activities resume. These two fish species are sensitive indicators of metal pollution in the marine environment and it is important to take measures to reduce the release of contaminating metals into the environment. The need to adopt sustainable tourism practices and stricter environmental regulations to protect marine ecosystems is also mentioned.

Efficiency of clustering methods and self-organizing maps of Adriatic sardines and anchovies regarding organochlorine and fatty acid burden.

The Adriatic Sea plays a crucial role as both a significant fishing ground and a thriving trading market for small pelagic edible fish. Recognized for their nutritional value, these fish are esteemed for their high protein content and abundance of polyunsaturated omega-3 and omega-6 fatty acids, making them a sought-after and healthful food choice. Nevertheless, pelagic species can also serve as a reservoir for lipophilic organochlorine pollutants, posing potential risks to human health. In this study, we compared traditional classification methods traditional principal component analysis (PCA) and Ward's clustering with an advanced self-organizing map (SOM) algorithm in determining distribution patterns of 24 organochlorines and 19 fatty acids in sardine and anchovy samples taken from the eastern Adriatic. The outcomes reveal the strengths and weaknesses of the three approaches (PCA, Ward's clustering, and SOM). However, it is evident that SOM has proven to be the most effective in offering detailed information and data visualization. Although sardines and anchovies exhibit similar distribution patterns for p,p'-DDE, PCB-28, PCB-138, PCB-153, PCB-118, and PCB-170, they differ in the concentrations of fatty acids such as stearic, palmitic, myristic, oleic, docosapentaenoic, and docosahexaenoic acid. Our findings supply valuable insights for environmental authorities and fish consumers concerning the potential risks associated with organochlorines in these two types of fish.

Life strategy of Antarctic silverfish promote large carbon export in Terra Nova Bay, Ross Sea

Antarctic silverfish Pleuragramma antarcticum is the most abundant pelagic fish in the High Antarctic shelf waters of the Southern Ocean, where it plays a pivotal role in the trophic web as the major link between lower and higher trophic levels. Despite the ecological importance of this species, knowledge about its role in the biogeochemical cycle is poor. We determine the seasonal contribution of Antarctic silverfish to carbon flux in terms of faeces and eggs, from samples collected in the Ross Sea. We find that eggs and faeces production generate a flux accounting for 41% of annual POC flux and that the variability of this flux is modulated by spawning strategy. This study shows the important role of this organism as a vector for carbon flux. Since Antarctic silverfish are strongly dependent on sea-ice, they might be especially sensitive to climatic changes. Our results suggest that a potential decrease in the biomass of this organism is likely to impact marine biogeochemical cycles, and this should be factored in when assessing Southern Ocean carbon budget.

Larval trophic ecology of small pelagic fishes: a review of recent advances and pathways to fill remaining knowledge gaps

Early life stages constitute a bottleneck for most fish populations, particularly for small pelagic fish (SPF), for which the interannual variability in recruitment strength is very high, and recruits frequently constitute the bulk of the population biomass. Finding the right prey (in terms of size and quality) during these early stages is critical for recruitment success. In this work, we synthesize the available literature on the trophic ecology of the early life stages of SPF, particularly clupeiforms. Works published during the last decade (2013-2022, 37 papers) were compared to those published previously (1920-2012, 107 papers). Gut content analysis of field-caught larvae is still the most commonly used technique (44%), while the use of biomarkers (e.g. stable isotopes and fatty acid composition), molecular tools (e.g. metabarcoding) and multitrophic approaches has increased in the last decade. Significant new knowledge was gained recently, such as that on larval feeding rates and behavior through laboratory experiments for species kept in culture (e.g. Atlantic herring, Pacific and Atlantic sardines), but some old challenges remain, such as the high vacuity rates of field-caught larvae. Lastly, we provide recommendations for future studies, such as the use of complementary techniques, the importance of studying ontogenetic shifts, the use of metabarcoding for analyzing the diet of early larvae that depend on microplankton, and the identification of prey with high taxonomic resolution. Such studies are essential to better understand larval growth and survival at sea, and thus to better understand and predict SPF population dynamics.

Pelagic fish spared from ocean catch by integrating Black Soldier Fly Larvae in U.S. aquaculture production

The sustainability challenges associated with utilizing forage fish sourced from ocean catch as fish meal and fish oil in the aquaculture industry has increased the demand for alternative feeds. Previous research indicates that Black Soldier Fly Larvae (BSFL; Hermetia illucens) can partially replace fish meal and/or fish oil in the diets of farmed aquaculture species without compromising fish growth or efficiency. The objective of our study was to identify the amount of pelagic fish from ocean catch that could be spared from fish meal and fish oil production by integrating BSFL in the diets of three aquaculture species, Atlantic Salmon (Salmo salar), Rainbow Trout (Oncorhynchus mykiss), and Whiteleg Shrimp (Litopenaeus vannamei) in the United States. Annual output for these aquaculture species was collected for 2017–2019. For each specie, we calculated the total metric tons (MT) of spared pelagic fish, by taxa, from fish meal and fish oil production based on total lifetime food intake and dietary replacement rates of fish meal and fish oil by BSFL as established in previous literature. At the highest level of dietary BSFL substitution for fish meal and/or fish oil that did not sacrifice performance of the three aquaculture species, 40,843 MT of pelagic fish could be spared from ocean catch in the U.S. per year. Therefore, integrating BSFL in the diets of aquaculture species could reduce the demand for pelagic fish sourced from ocean catch and positively contribute to the sustainability of aquaculture production.

Occurrence of Antimicrobial-Resistant Bacteria in Intestinal Contents of Wild Marine Fish in Chile.

Antimicrobial-resistant bacteria (ARB) from the intestinal contents of wild fish may have a relevant ecological significance and could be used as indicators of antimicrobial-resistance dissemination in natural bacterial populations in water bodies impacted by urban contamination. Thus, the occurrence of ARB in the intestinal contents of pelagic and demersal wild fishes captured in anthropogenic-impacted Coquimbo Bay in Chile was studied. Culturable counts of total and antimicrobial-resistant bacteria were determined by a spread plate method using Trypticase soy agar and R2A media, both alone and supplemented with the antimicrobials amoxicillin, streptomycin, florfenicol, oxytetracycline and ciprofloxacin, respectively. Heterotrophic plate counts of pelagic and demersal fishes ranged from 1.72 × 106 CFU g-1 to 3.62 × 109 CFU g-1, showing variable proportions of antimicrobial resistance. Representative antimicrobial-resistant isolates were identified by 16S rRNA gene sequencing, and isolates (74) from pelagic fishes mainly belonged to Pseudomonas (50.0%) and Shewanella (17.6%) genera, whereas isolates (68) from demersal fishes mainly belonged to Vibrio (33.8%) and Pseudomonas (26.5%) genera. Antimicrobial-resistant isolates were tested for susceptibility to 12 antimicrobials by an agar disk diffusion method, showing highest resistance to streptomycin (85.2%) and amoxicillin (64.8%), and lowest resistance to oxytetracycline (23.2%) and ciprofloxacin (0.7%). Only furazolidone and trimethoprim/sulfamethoxazole were statistically different (p < 0.05) in comparisons between isolates from pelagic and demersal wild fishes. Furthermore, an important number of these isolates carried plasmids (53.5%) and produced Extended-Spectrum-β-lactamases (ESBL) (16.9%), whereas the detection of Metallo-β-Lactamases and class 1-integron was rare. This study provides evidence that wild fish are important reservoirs and spreading-vehicles of ARB, carrying plasmids and producing ESBLs in Chilean marine environments.