Peak Instantaneous Frequency Research Articles

Individual Fish Echo Detection Method Based on Peak Delay Estimation and Instantaneous Frequency Characterization

In fisheries science research and farmed fish monitoring, acquiring individual fish echoes is the basis for the growth assessment, tracking, and target strength estimation of farmed fish. However, individual fish echo detection methods based on narrowband signal features cannot be applied well to high-density aquaculture scenarios. The broadband signaling system can improve the distance resolution of the detected target and can help to improve the performance of individual fish echo detection. In this study, for the broadband signal system and the characteristics of the underwater fish acoustic echoes, an individual fish echo detection method is proposed using the matched filter output envelope peak interval and instantaneous frequency characteristics of the echo as evaluation indices, and the simulation and experiments of the method are carried out in an anechoic water tank. The results show that the broadband signal system and the corresponding detection method perform better in detecting single target echoes than the narrowband signal system. Compared with the broadband single echo detection method that only relies on the peak interval of the matched filter envelope, the joint detection method that incorporates the instantaneous frequency characteristics of the echo signal has a better rejection capability for overlapping echoes, which can reduce the probability of misjudging the overlapping echoes. The combined detection methods may provide a better detection performance for individual fish echoes.

Time-frequency analysis of air-coupled GPR data for identification of delamination between pavement layers

Identification of delamination in pavement layers using ground penetrating radar (GPR) is still challenging due to its limited range resolution. This paper investigates the characteristics of the composite reflection from the air-filled delamination between pavement layers by time-frequency analysis. We firstly simulated GPR data from an asphalt pavement model with an embedded air gap of different thicknesses by the Green’s function method. Then we conducted a laboratory experiment on a delaminated asphalt pavement model. Both the numerical and laboratory experiment results demonstrate that both the peak instantaneous frequency and its amplitude are sensitive to the variation of the air gap thickness. When the thickness of the air gap is smaller than a quarter of the dominant wavelength, the peak instantaneous frequency of the composite reflection is higher than that of the normal interface reflection. We conclude that we can identify the delamination from the overall rise in the peak instantaneous frequency.

Activation of muscarinic receptors by ACh release in hippocampal CA1 depolarizes VIP but has varying effects on parvalbumin-expressing basket cells.

Optogenetically released acetylcholine (ACh) from medial septal afferents activates muscarinic receptors on both vasoactive intestinal peptide-expressing (VIP) and parvalbumin-expressing (PV) basket cells (BCs) in mouse hippocampal CA1. ACh release depolarized VIP BCs whereas PV BCs depolarized, hyperpolarized or produced biphasic responses. Depolarizing responses in VIP or PV BCs resulted in increased amplitudes and frequencies of spontaneous inhibitory postsynaptic currents (sIPSCs) in CA1 pyramidal neurons. The instantaneous frequency of sIPSCs that result from excitation of VIP or PV BCs primarily occurred within the low gamma frequency band (25-50 Hz). We investigated the effect of acetylcholine release on mouse hippocampal CA1 perisomatically projecting interneurons. Acetylcholine was optogenetically released in hippocampal slices by expressing the excitatory optogenetic protein oChIEF-tdTomato in medial septum/diagonal band of Broca cholinergic neurons using Cre recombinase-dependent adeno-associated virally mediated transfection. The effect of optogenetically released acetylcholine was assessed on interneurons expressing Cre recombinase in vasoactive intestinal peptide (VIP) or parvalbumin (PV) interneurons using whole cell patch clamp methods. Acetylcholine released onto VIP interneurons that innervate pyramidal neuron perisomatic regions (basket cells, BCs) were depolarized by muscarinic receptors. Although PV BCs were also excited by muscarinic receptor activation, they more frequently responded with hyperpolarizing or biphasic responses. Muscarinic receptor activation resulting from ACh release increased the frequency of spontaneous inhibitory postsynaptic currents (sIPSCs) in downstream hippocampal CA1 pyramidal neurons with peak instantaneous frequencies occurring in both the gamma and theta bandwidths. Both PV and VIP BCs contributed to the increased sIPSC frequency in pyramidal neurons and optogenetic suppression of PV or VIP BCs inhibited sIPSCs occurring in the gamma range. Therefore, we propose acetylcholine release in CA1 has a complex effect on CA1 pyramidal neuron output through varying effects on perisomatically projecting interneurons.

Postsynaptic odorant concentration dependent inhibition controls temporal properties of spike responses of projection neurons in the moth antennal lobe.

Although odorant concentration-response characteristics of olfactory neurons have been widely investigated in a variety of animal species, the effect of odorant concentration on neural processing at circuit level is still poorly understood. Using calcium imaging in the silkmoth (Bombyx mori) pheromone processing circuit of the antennal lobe (AL), we studied the effect of odorant concentration on second-order projection neuron (PN) responses. While PN calcium responses of dendrites showed monotonic increases with odorant concentration, calcium responses of somata showed decreased responses at higher odorant concentrations due to postsynaptic inhibition. Simultaneous calcium imaging and electrophysiology revealed that calcium responses of PN somata but not dendrites reflect spiking activity. Inhibition shortened spike response duration rather than decreasing peak instantaneous spike frequency (ISF). Local interneurons (LNs) that were specifically activated at high odorant concentrations at which PN responses were suppressed are the putative source of inhibition. Our results imply the existence of an intraglomerular mechanism that preserves time resolution in olfactory processing over a wide odorant concentration range.

Seismic Attenuation Estimation From Instantaneous Frequency

<para xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> An approach is proposed for quality (<formula formulatype="inline"><tex Notation="TeX">$Q$</tex></formula>) factor estimation from the variation of envelope peak instantaneous frequency (EPIF). For a frequency-independent <formula formulatype="inline"><tex Notation="TeX">$Q$</tex></formula> model, assuming that the propagating wavelet can be modeled by a Gaussian function with constant phase, an approximate analytic relation between <formula formulatype="inline"> <tex Notation="TeX">$Q$</tex></formula> and EPIF variation is derived. Synthetic tests show that the EPIF method has higher resolution and is less sensitive to noise and interference reflection than common methods. The field test of reflection seismic data indicates that the zone of lower <formula formulatype="inline"><tex Notation="TeX">$Q$</tex></formula> -factors corresponds well to the gas reservoir. </para>

Seismic Attenuation Tomography by Envelope Peak Instantaneous Frequency

This letter describes an attenuation tomography method via variations in the envelope peak instantaneous frequencies (EPIFs) of seismic signals. For a linear frequency-attenuation model, assuming that the propagating wavelet can be modeled by a Gaussian function with constant phase, an imaging formula using EPIF variation is derived. In tomography, the simultaneous iterative reconstruction technique (SIRT) with travel-time weighting is proposed for tomographic inversion. Numerical simulations of both noise-free and noise-added data show the validity of attenuation tomography from EPIF variation. SIRT with travel-time weighting yields a better reconstruction and lower residual.

Discharge patterns of somatosensitive neurons in the nucleus tractus solitarius of the cat

Encoding of sensory information by nucleus of the solitary tract (NTS) neurons is incompletely understood. Using extracellular single-unit recording in α-chloralose-urethane anesthetized cats, we have examined the discharge characteristics of NTS neurons to activation of somatic Aδ and C fiber afferents by skeletal muscle contraction evoked by electrical stimulation of lower lumbar/upper sacral ventral roots. Generally, somatic afferent stimulation evoked two distinct firing patterns. The first population (36/43 cells) increased their firing rate to brief somatic stimuli. A subset (21/27 cells) exhibited a rapid decay of their firing rate during sustained somatic stimulation. Peak instantaneous firing frequency ( F p) increased proportionally with the intensity of somatic stimulation (105±4 vs. 119±4 vs. 139±4 Hz, 10, 20 and 40 Hz, respectively, P<0.0001), whereas steady-state firing frequency ( F ss) was not altered (25±2 vs. 27±2 vs. 27±2 Hz, 10, 20 and 40 Hz, respectively, P=0.72). Two indices were derived to quantify the decay properties. The decay rate constant (obtained from exponential curve fitting) was not altered by stimulation frequency (461±10 vs. 442±14 vs. 429±26 ms, 10, 20 and 40 Hz, respectively, P=0.415), nor was the decay index (derived to express the percent reduction in firing rate with respect to the initial peak firing rate; 76±2 vs. 77±2 vs. 81±2%, 10, 20 and 40 Hz, respectively, P=0.187). In contrast, the second population (seven of 43 cells) decreased their firing rate to stimulation. Of the NTS neurons tested for barosensitivity (29/36), none responded to pressure stimulation. These results have identified a population of somatosensitive NTS neurons that exhibit rapid firing rate decay properties during sustained stimulation. However, this population could faithfully encode phasic excitation during rhythmic somatosensory input. These results are discussed in relation to the role of somatosensory input on baroreflex function.

Gliding behaviour elicited by lateral looming stimuli in flying locusts

We challenged tethered, flying locusts with visual stimuli looming from the side towards one eye in a way that mimics the approach of a predatory bird. Locusts respond to the lateral approach of a looming object with steering movements and a stereotyped, rapid behaviour in which the wingbeat pattern ceases and the wings are swept into a gliding posture. This gliding behaviour may cause the locust to dive. The gliding posture is maintained for 200 ms or more after which flight is resumed with an increased wingbeat frequency or else the wings are folded. A glide begins with a strong burst of activity in the mesothoracic second tergosternal motor neuron (no. 84) on both sides of the locust. Recordings of descending contralateral movement detector (DCMD) activity in a flying locust show that it responds to small (80-mm diameter) looming stimuli during tethered flight, with a prolonged burst of spikes that tracks stimulus approach and reaches peak instantaneous frequencies as, or after, stimulus motion ceases. There is a close match between the visual stimuli that elicit a gliding behaviour and those that are effective at exciting the DCMD neuron. Wing elevation into the gliding posture occurs during a maintained burst of high frequency DCMD spikes.

Compressional‐wave Q estimation from full‐waveform sonic data

There is significant evidence that the anelastic loss of seismic energy is linked to petrophysical properties such as porosity, permeability and clay content. Thus, reliable estimation of anelastic attenuation from seismic data can lead to improved methods for the prediction of petrophysical properties. This paper is concerned with methods for the estimation of attenuation at sonic frequencies (5–30 KHz) from in situ data. Two independent methods have been developed and tested for estimating compressional‐wave attenuation from full‐waveform sonic data. A well‐established technique, the logarithm spectral ratio (LSR) method, is compared with a new technique, the instantaneous frequency (IF) method. The LSR method uses the whole spectrum of the seismic pulse whilst the IF method uses a carefully estimated value of instantaneous frequency which is representative of the centre frequency of the pulse. In the former case, attenuation estimation is based on the relative variation of amplitudes at different frequencies, whilst in the latter case it is based on the shift of the centre frequency of the pulse to lower values during anelastic wave propagation. The IF method does not assume frequency independence of Q which is a necessary assumption for the LSR method, and it provides a stable frequency log, the peak instantaneous frequency (PIF) log, which may be used as an indicator for attenuation under certain limitations. The development and implementation of the two methods is aimed at minimizing the effect of secondary arrivals, such as leaky modes, and involved a series of parameter tests. Testing of the two methods using full‐waveform sonic data of variable quality, obtained from a gas‐bearing sandstone reservoir, showed that the IF method is in general more stable and suitable for full‐waveform sonic data compared with the LSR method. This was evident especially in data sets with high background noise levels and wave‐interference effects. For good quality data, the two methods gave results that showed good agreement, whilst comparison with other log types further increased confidence in the results obtained. A significant decrease (approximately 5 KHz) in the PIF values was observed in the transition from an evaporite/shale sequence to the gas‐bearing sandstone. Average Q values of 54 and 51 were obtained using good quality data from a test region within the gas‐saturated sandstone reservoir, using the LSR and IF methods, respectively.

Glutamate-induced excitation and sensitization of nociceptors in rat glabrous skin

Anatomical studies demonstrate the presence of glutamate receptors on unmyelinated axons in peripheral cutaneous nerves. Pharmacological studies show that intraplantar injection of glutamate or glutamate agonists in the glabrous skin results in nociceptive behaviors. The present study describes a novel in vitro skin-nerve preparation using the glabrous skin from the rat hindpaw. In the first series of experiments, recordings were obtained from 141 fibers that responded to a strong mechanical search stimulus. Based on their conduction velocity they were classified as C (27%), Aδ (28%) and Aβ (45%) fibers. The C and Aδ fibers typically exhibited sustained firing during suprathreshold mechanical stimuli whereas both rapidly (66%) and slowly (34%) adapting responses were obtained from Aβ fibers. Noxious heat excited 46% of the C fibers but only 12% of the Aδ units. In another series of experiments application of an ascending series of glutamate concentrations (10, 100, 300, and 1000 μM) to Aδ ( n=14) and C ( n=19) nociceptors resulted in a significant excitation of 43% (6/14) Aδ fibers and 68% (13/19) C fibers. At these concentrations, there was no excitation of Aβ units ( n=13). Superfusion of the receptive fields of either mechanoheat-sensitive A (AMH, n=10) or C fibers (CMH, n=12) for 2 min with 300 μM glutamate resulted in sensitization of 90% (9/10) AMH and 92% (11/12) CMH fibers to subsequent thermal stimulation. This was evidenced by a significant (1) decrease in thermal threshold for activation, (2) increase in discharge rate, and (3) increase in peak instantaneous frequencies during the second heat trial. Glutamate-induced sensitization to heat occurred in the absence of either a glutamate-induced excitation or an initial heat response. Exposure of Aδ or C fibers to glutamate did not result in a decrease in von Frey thresholds. These data provide a physiological basis for the nociceptive behaviors that arise following intraplantar injection of glutamate or glutamate agonists. Furthermore, demonstration of glutamate-induced excitation and heat sensitization of nociceptors indicates that local or topical administration of glutamate receptor antagonists may have therapeutic potential for the treatment of pain.

Spontaneous and evoked ectopic discharges recorded from single human axons.

A quantitative assessment was made of the firing characteristics of repetitive axonal discharges encountered during microneurographic recordings from human peripheral nerves. Spontaneous activity was recorded from 16 single axons using tungsten microelectrodes inserted percutaneously into fascicles of the median or peroneal nerves in normal subjects. These discharges typically consisted of brief bursts of 2-5 spikes occurring at a frequency of 7-10 Hz. Peak instantaneous frequencies usually exceeded 300 Hz. Based on their similarity with spontaneous high-frequency discharges recorded from single axons following nerve damage, ischemia, prolonged electrical stimulation, or hyperventilation, it is concluded that they are generated ectopically at the site of a previous impalement of a nerve fiber. It is suggested that short-term damage to the nerve fiber caused by the microelectrode may allow accumulation of K+ underneath the myelin, triggering an inward flow of K+ and regenerative depolarizations. Alternatively, internodal channels may be exposed following damage to the myelin, resulting in the generation of spontaneous pacemaker potentials and repetitive discharges.

Dynamic responses of series force receptors innervating the opener muscle apodeme in the blue crab, Callinectes sapidus.

Receptors monitoring muscle force innervate the opener muscle apodeme in the walking legs of the blue crab, Callinectes sapidus. Biocytin backfills reveal 9-15 bipolar neurons with somata as large as 60 micrometer m positioned at the distal end of the apodeme. Sensory endings insert into the apodeme and are in series with the opener muscle. The axons of these neurons form the opener apodeme sensory nerve that merges with the most distal branch of the opener motor nerve. Recordings reveal that the receptors are not spontaneously active nor do they respond to passive muscle stretch. Isometric muscle contraction evoked by stimulating the opener excitor motor neuron is the adequate stimulus for receptor firing. Most significant is the finding that during contraction, over a wide range of forces, the firing rate of individual receptors closely parallels the rate of change of isometric force. The peak instantaneous frequency typically occurs at the force derivative maximum, but not at maximum force development. Thus, receptors of the opener apodeme sensory nerve more closely monitor changes in isometric force rather than the total force achieved.

An ionic current model for neurons in the rat medial nucleus tractus solitarii receiving sensory afferent input.

1. Neurons from a horizontal slice of adult rat brainstem were examined using intracellular recording techniques. Investigations were restricted to a region within the nucleus tractus solitarii, medial to the solitary tract and centred on the obex (mNTS). Previous work has shown this restricted area of the NTS to contain the greatest concentration of aortic afferent baroreceptor terminal fields. Electrical stimulation of the tract elicited short-latency excitatory postsynaptic potentials in all neurons. 2. mNTS neurons were spontaneously active with firing frequencies ranging between 1 and 10 Hz, at resting potentials of -65 to -45 mV. These neurons did not exhibit spontaneous bursting activity. 3. Depolarizing current injection immediately evoked a finite, high-frequency spike discharge which rapidly declined to a lower steady-state level (i.e. spike frequency adaptation, SFA). Increasing depolarizations produced a marked increase in the peak instantaneous frequency but a much smaller increase in the steady-state firing level. 4. Conditioning with a hyperpolarizing prepulse resulted in a prolonged delay of up to 600 ms before the first action potential (i.e. delayed excitation, DE) with an attendant decrease in peak discharge rates. DE was modulated by both the magnitude and duration of the prestimulus hyperpolarization, as well as the magnitude of the depolarizing stimulus. Tetrodotoxin (TTX) eliminated spike discharge but had little effect on the ramp-like membrane depolarization characteristic of DE. 5. We have developed a mathematical model for mNTS neurons to facilitate our understanding of the interplay between the underlying ionic currents. It consists of a comprehensive membrane model of the Hodgkin-Huxley type coupled with a fluid compartment model describing cytoplasmic [Ca2+]i homeostasis. 6. The model suggests that (a) SFA is caused by an increase in [Ca2+]i which activates the outward K+ current, IK,Ca, and (b) DE results from the competitive interaction between the injected depolarizing current and the hyperpolarization-activated transient outward K+ currents, IA and ID. 7. We conclude that our ionic current model is capable of providing biophysical explanations for a number of phenomena associated with brainstem neurons, either during spontaneous activity or in response to patterned injections of current. This model is a potentially useful adjunct for on-going research into the central mechanisms involved in the regulation of both blood pressure and ventilation.

Deterrence coding by a larval Manduca chemosensory neurone mediating rejection of a non‐host plant, Canna generalis L.

Abstract. The physiological basis of phagodeterrence was studied electrophy‐siologically and behaviourally in the phytophagous caterpillars Manduca sexta and Manduca quinquemaculata. The model unacceptable non‐host plant was the canna lily, Canna generalis.A strongly deterrent extract was obtained from fresh leaves of canna by extraction with hot ethanol or ethyl acetate in a blender. Behavioural rejection of these extracts was similar to that of fresh leaves, although less intense. In contrast, blender extracts using other solvents, as well as leaf surface rinses, were phagostimulant or neutral. Chromatographic fractionation of the deterrent ethanolic extract showed the active principles to be moderately polar and separable into two fractions.Previous ablation experiments had shown that the medial maxillary styloconica and epipharyngeal sensilla are the two most important chemosensory organs in mediating behavioural rejection of canna leaves; if only one of these organs is spared, the animal completely rejects canna. We investigated the neural responses of the medial styloconica and their contribution to the sensory coding responsible for this phagodeterrence.The active fractions of the deterrent ethanolic extract elicited a vigorous response from one chemosensory neurone in the medial styloconica. This neurone is distinguishable from others in the medial styloconica by its unique temporal response parameters and the characteristic shape changes of its action potentials. The response frequency of this neurone correlates with the degree of phagodeterrence in a dose‐dependent manner. Threshold deterrence occurs at a concentration of extract (1%) that elicits firing in this neurone at a rate of c. 50 spikes/s peak instantaneous frequency and 30 total spikes in the first Is.We conclude that this is a ’deterrent neurone’ in the sense that vigorous response from this neurone is a sufficient sensory code for behavioural rejection of canna. Thus input from a single sensory neurone is capable of blocking feeding, since only one (unilateral) medial styloconicum is needed to mediate this rejection.

Activity-dependent induction of facilitation, depression, and post-tetanic potentiation at an insect central synapse

In Manduca sexta larvae, sensory neurons innervating planta hairs on the tips of the prolegs make monosynaptic excitatory connections with motoneurons innervating proleg retractor muscles. Tactile stimulation of the hairs evokes reflex retraction of the proleg. In this study we examined activity-dependent changes in the amplitude of the excitatory postsynaptic potentials (EPSPs) evoked in a proleg motoneuron by stimulation of individual planta hair sensory neurons. Deflection of a planta hair caused a phasic-tonic response in the sensory neuron, with a mean peak instantaneous firing frequency of greater than 300 Hz, and a tonic firing rate of 10-20 Hz. Direct electrical stimulation was used to activate individual sensory neurons to fire at a range of frequencies including those observed during natural stimulation of the hair. At relatively low firing rates (e.g., 1 Hz), EPSP amplitude was stable indefinitely. At higher instantaneous firing frequencies (greater than 10 Hz), EPSPs were initially facilitated, but continuous stimulation led rapidly to synaptic depression. High-frequency activation of a sensory neuron could also produce post-tetanic potentiation, in which EPSP amplitude remained elevated for several min following a stimulus train. Facilitation, depression, and post-tetanic potentiation all appeared to be presynaptic phenomena. These activity-dependent changes in sensory transmission may contribute to the behavioral plasticity of the proleg withdrawal reflex observed in intact insects.

Muscle sounds are emitted at the resonant frequencies of skeletal muscle

The changes in mechanical resonant frequency of whole muscles during twitch and tetanic contractions were compared to changes in frequency components of the pressure wave produced by muscles during contraction. Resonant frequencies were determined by imposing sinusoidal length changes on a muscle and observing transverse standing waves when the frequency of length change matched the muscle's resonant frequency or a harmonic of the resonant frequency. Acoustic signal instantaneous frequency spectrums were calculated using time-frequency transformations including the Wigner transform and the exponential distribution. During a tetanic muscle contraction, the peak instantaneous frequency initially increased and then became constant as the force plateau was reached. The resonant frequencies determined during the force plateau and during relaxation spanned the same range as the peak instantaneous frequency of the acoustic signal. These results suggest that the acoustic signal may be useful as a non-invasive monitor of muscle resonant frequency during contraction.

Repetitive firing properties of phrenic motoneurons in the cat.

1. Using both rectangular- and ramp-shaped intracellularly injected currents, the repetitive firing properties of 23 antidromically identified phrenic motoneurons were determined in anesthetized, paralyzed, and artificially ventilated cats during hypocapnic apnea. In response to rectangular depolarizing current injections, regular repetitive firing was observed in all cells. 2. At the beginning of a rectangular current pulse, the firing pattern was characterized by high frequency of firing that rapidly adapted to a much lower steady-state value. The relationship between the reciprocal of the first interspike interval (F1-2) and injected current was described by an initial linear portion of shallow slope, followed by a much steeper segment that smoothly reached a plateau value. The plateau value of F1-2 did not change with further increase in injected current. 3. The steady-state firing frequency versus injected current relationship was represented by a line of shallow slope over the entire range of injected currents. The slope of this line ranged between 1.1 and 4.5 Hz/nA. 4. A weaker correlation between minimal firing frequency for continuous activity and the reciprocal of the after hyperpolarization duration (1/AHPdur) was found for phrenic motoneurons than exists for lumbosacral motoneurons (26). Comparison of AHP shape at different levels of repetitive firing revealed that the slopes of the ascending portions of the AHP were similar except at the higher injected currents. Further, in the same cells during natural inspiratory activity the ascending part of the AHP was similar to that observed during current injection. 5. Depolarizing current ramps (approximately 1-s duration) were injected into 11 phrenic motoneurons. Instantaneous firing frequency was directly proportional to the intensity of the instantaneous injected current and independent of the rate of change of current for the range of ramp slopes tested (5-80 nA/s). Ramp-and-hold current injections were done in three motoneurons, and the peak instantaneous firing frequency showed little adaptation during the hold maneuver. 6. During hypocapnic apnea, we mimicked the expiratory-phase inhibition and inspiratory-phase excitation of phrenic motoneurons by injecting a 1-s depolarizing current ramp that was immediately preceded by a 1-s hyperpolarizing current ramp of the same absolute peak current intensity. Compared with the effects of positive current ramps alone the spike onsets during the negative-positive current ramp paradigm were either facilitated or retarded. Various ionic mechanisms are suggested for these effects as well as their function in determining the onset of firing during natur

Activity of neurons located in the region of the hypoglossal motor nucleus during swallowing in sheep.

Extracellular activity of swallowing neurons (SN) in the region of the hypoglossal (XIIth) motor nucleus was studied in sheep anesthetized with halothane. Eighty six SN exhibited a discharge closely linked to swallowing electromyographic (EMG) activity of the geniohyoïd (GH) muscle induced by stimulation of the superior laryngeal nerve. Swallowing activation persisted after motor paralysis, indicating that this activity did not result from sensory feedback. SN were classified into two groups. Group I SN (N = 66) discharged a burst of up to 12 spikes for 50-300 ms during the response of GH. Mean frequencies ranged from 10 to 60 Hz, peak instantaneous frequencies from 10 to 100 Hz. Thirty two SN were antidromically activated by stimulating the XIIth nerve. Mean latencies of antidromic spikes were 2.6 ms and 2.4 ms for SN sending their axons in the medial and lateral branches respectively of XIIth nerve, corresponding to conduction velocities of 50.4 m/s and 53.7 m/s. The other 34 group I SN were located at sites with large antidromic field potentials obscuring the identification of unitary antidromic spikes. Thirty group I SN, 16 antidromically activated and 14 in areas with large field potentials, were histologically located in the XIIth motor nucleus between the transverse planes 1 mm caudal to 2 mm rostral to the obex. Group I SN are likely motoneurons supplying GH and tongue musculature. Group II SN (N = 20) were never antidromically activated by XIIth nerve stimulation, and were all located in the reticular formation adjacent to the lateral edge of the XIIth motor nucleus, particularly in transverse planes within 1 mm of the obex.(ABSTRACT TRUNCATED AT 250 WORDS)

Thin Bed Stratigraphy from Complex Trace Attributes: ABSTRACT

Both model seismic data and broad-band field data acquired to delineate complicated stratigraphy have been converted to displays of the instantaneous attributes of the complex seismic trace. Attribute sections enhance the interpretation of conventional sections not only by qualitatively highlighting specific properties of conventional displays, but also by quantitatively defining wavelet characteristics like dominant frequency, and stratigraphic variables like formation thickness. An example of the quantitative use of complex attributes in wavelet definition is the phenomenon that the maximum instantaneous frequency of a zero-phase Ricker wavelet is synchronous with the central peak of the wavelet and exactly equal to the frequency corresponding to the center of gravity o the wavelet's amplitude spectrum. Peak instantaneous frequency thus is a physical meaningful measure of the spectral content of a zero-phase Ricker wavelet. If the signal in a seismic section can be approximated by zero-phase Ricker wavelets, and if the geophysicist can identify occasional wavelet peaks in the sections which are uncontaminated by noise or interference, instantaneous frequencies at these samples are direct estimates of a significant and absolute spectral characteristic of the signal. An example of the quantitative use of attribute sections in seismic stratigraphy is their application to estimation of the thickness of thin, porous sands. Pods of porous sand which are encased in high-velocity material and whose thicknesses are of the order of half the peak-to-peak period of the dominant seismic energy show up as anomalously high amplitude zones on instantaneous amplitude displays. These anomalies result from the well-known amplitude tuning effect which occurs when reflection coefficients of opposite polarity a half-period apart are convolved with a seismic wavelet. As sand members thin to a quarter-period of the dominant seismic energy, the thinning is revealed by an anomalous increase in instantaneous frequency. This behavior results from the less well-known but eq ally important phenomenon of frequency tuning by thinning beds. Frequency tuning reaches a maximum when sand thickness is about a quarter-period and remains evident as the sand continues to thin. The instantaneous frequency section thus can be a sensitive analytical tool for investigating stratigraphic sequences composed of very thin layers. Frequency and amplitude tunings are accompanied by changes in the character of the complex of interfering reflections from various impedance boundaries in a formation of thin beds, and these changes are highlighted by the instantaneous phase display. End_of_Article - Last_Page 624------------

The physiologically relevant information regarding systemic blood pressure encoded in the carotid sinus baroreceptor discharge pattern.

1. The objective was to find out what kind of informatioon regarding systemic blood pressure is transduced by baroreceptors in vivo and how this information is coded in the receptor discharge. 2. Carotid sinus pressure, e.c.g., and receptor action potentials were recorded for fifty-two single fibre carotid sinus receptors found in twenty decerebrated unanaesthetized cats. 3. The inflation and gradual deflation of an intraaortic catheter tip balloon manipulated the blood pressure in the carotid sinus in a way as to define the full in vivo stimulus-response curve for each receptor. 4. Correlation coefficients were computed between stimulus and response variables for several points on the response curve of each receptor and for every possible combination of stimulus and response variables defined. 5. Stimulus variables were (a) systolic, (b) diastolic,, (c) mean, (d) pulse pressures and (e) peak positive dP/dt. Response variables were (a) average discharge rat, (b) peak instantaneous frequency, and (c) average burst frequency. 6. For every fibre in the sample only the correlations between systolic, diastolic and mean pressures vs. average discharge rate were consistently high and positive. All other correlations were numerically low and/or negative. 7. It was concluded that in vivo baroreceptors signal mainly pressure level (systolic, diastolic or mean) as opposed to pulse pressure or dP/dt, and that the average discharge rate is their best index of information content.