We present a new determination of the Cabibbo-Kobayashi-Maskawa matrix element |Vcb| by using the three-loop perturbative QCD corrections for the B→D⁎ semi-leptonic decay. The decay width of B→D⁎ semi-leptonic decay can be factorized as perturbatively calculable short-distance part and the non-perturbative but universal long-distance part. We adopt the principle of maximum conformality (PMC) single-scale setting approach to deal with the perturbative series so as to achieve a precise fixed-order prediction for the short-distance parameter ηA. By applying the PMC, an overall effective αs value is achieved by recursively using the renormalization group equation, which inversely results in a precise scale-invariant pQCD series. Such scale-invariant series also provides a reliable basis for predicting the contributions from uncalculated perturbative terms. We then obtain ηA=0.9225−0.0168+0.0117, where the error is the squared average of those from Δαs(MZ)=±0.0010 and the uncertainties caused by the uncalculated higher-order perturbative terms. By using the data of B→D⁎ℓν¯ℓ, we finally obtain |Vcb|PMC=(40.60−0.57+0.53)×10−3, which is consistent with the PDG value within errors.