Paw Preference Research Articles

Laterality Handedness When Completing a Tool-Use Task in Asian Small-Clawed Otters (Aonyx cinerea)

Laterality is defined as the occurrence of each cerebral hemisphere having asymmetric control over the different sides of the body, leading to preferences to use one side of the body over the other for various behaviors. Many types of laterality exist, but handedness is the form that is most predominantly assessed. Handedness in animals is of special interest to laterality researchers, as humans were once thought to be the only species that exhibited lateralized hand preferences. The aim of the current study was to determine if Asian small-clawed otters (Aonyx cinerea) exhibit significant paw preferences in behaviors associated with completing a specific tool-use task. Video data of the otters completing the task was analyzed for specific paw interactions with the tool-use apparatus, and the percentage of correct completion of the task was documented for each otter. Overall, no significant population level paw preference was present. However, individual differences in paw preference and differing trends in handedness on each focal behavior was present. The results showed that paw preference (or lack thereof) did affect the tool-use task completion for some otters, and for others, the preference did not seem to have an effect. The main implications of this study are that paw preferences in Asian small-clawed otters are present on an individual level when solving a novel tool-use task.

Laterality Handedness When Completing a Tool-Use Task in Asian Small-Clawed Otters (Aonyx cinerea)

Laterality is defined as the occurrence of each cerebral hemisphere having asymmetric control over the different sides of the body, leading to preferences to use one side of the body over the other for various behaviors. Many types of laterality exist, but handedness is the form that is most predominantly assessed. Handedness in animals is of special interest to laterality researchers, as humans were once thought to be the only species that exhibited lateralized hand preferences. The aim of the current study was to determine if Asian small-clawed otters (Aonyx cinerea) exhibit significant paw preferences in behaviors associated with completing a specific tool-use task. Video data of the otters completing the task was analyzed for specific paw interactions with the tool-use apparatus, and the percentage of correct completion of the task was documented for each otter. Overall, no significant population level paw preference was present. However, individual differences in paw preference and differing trends in handedness on each focal behavior was present. The results showed that paw preference (or lack thereof) did affect the tool-use task completion for some otters, and for others, the preference did not seem to have an effect. The main implications of this study are that paw preferences in Asian small-clawed otters are present on an individual level when solving a novel tool-use task.

Development of Hand and Paw Preferences and Their Association with Other Patterns of Behaviour and Cognition

Hand preference in non-human primates has been studied extensively with the aim of understanding the evolution of hemispheric asymmetry and hand preferences in humans. However, the focus has been on hand preferences expressed in adulthood, with a surprising lack of studies on hand preferences in infants and changes that occur during the development of other, potentially associated, asymmetries in the brain and behaviour. This paper reports on the development of hand preference for grasping food and taking it to the mouth in common marmosets. It considers the development of other types of behaviour, such as head cocking and anogenital licking, that parallel and might influence the development of hand preferences during the first months of life. It then discusses behavioural differences between left- and right-handed adult marmosets, including response to novel stimuli, social behaviour and cognitive bias. The need to study the development of hand preferences together with the development of these other expressions of cognitive function is highlighted. The question to be addressed by empirical studies is whether hand preference is a downstream manifestation of the development of hemispheric differences in sensory processing and cognition, or whether it is instrumental in the development of functional differences between the hemispheres. Comparison is made to paw preference and associated behaviour in non-primate species.

Paw preference as an indicator of operational suitability in working dogs: An ex post facto analysis

Paw laterality has been linked to personality and performance in dogs. Although some research has shown that right-pawed dogs are more aggressive and less fearful, the majority of research suggests that the degree of laterality is more predictive of behavior than of a simple preference for the right or left paw. In a retrospective study we analyzed laterality in dogs that were separated into two groups: a fully operational group and a semi-operational group. The decision as to which group was based on a behavioral assessment by the chief trainer. We selected dogs on the basis of availability from these two groups and measured the direction and extent of laterality using a WALK test and a COME test. In both tests we determined which paw was moved first. Our results revealed that most of the dogs showed a right-paw preference, with no difference between the operational and semi-operational dogs. However, regarding the extent of laterality, the semi-operational dogs showed significantly less laterality than the operational dogs. Given that laterality measures seem to be consistent over time (Tomkins et al., 2010b), we found that if laterality had been a criterion used when purchasing the dogs, our selection process would have been significantly improved.

Acute and chronic stress alter behavioral laterality in dogs

Dogs are one of the key animal species in investigating the biological mechanisms of behavioral laterality. Cerebral asymmetries are assumed to be influenced by stress, but this subject has not yet been studied in dogs. This study aims to investigate the effect of stress on laterality in dogs by using two different motor laterality tests: the Kong™ Test and a Food-Reaching Test (FRT). Motor laterality of chronically stressed (n = 28) and emotionally/physically healthy dogs (n = 32) were determined in two different environments, i.e., a home environment and a stressful open field test (OFT) environment. Physiological parameters including salivary cortisol, respiratory rate, and heart rate were measured for each dog, under both conditions. Cortisol results showed that acute stress induction by OFT was successful. A shift towards ambilaterality was detected in dogs after acute stress. Results also showed a significantly lower absolute laterality index in the chronically stressed dogs. Moreover, the direction of the first paw used in FRT was a good predictor of the general paw preference of an animal. Overall, these results provide evidence that both acute and chronic stress exposure can change behavioral asymmetries in dogs.

Does owner handedness influence paw preference in dogs?

Handedness has proven to be the most effective and least intrusive measure of laterality in many species. Several studies have investigated paw preference in dogs (Canis familiaris) without considering the potential impact that owner’s handedness may have on it, despite dogs being a domesticated species. The aim of this study was to investigate whether owner handedness influences paw preference in their dogs. Sixty-two dogs had their paw preference tested using a Paw Task and a Reach Task in their home over 10 days, recorded by their owners. Interestingly, it was found that left-handed owners were more likely to own a dog with a left paw bias, and right-handed owners were more likely to own a dog with a right paw bias. In the Paw Task, the hand presented to a dog did not significantly predict which paw the dog lifted in response. Furthermore, it was found that females displayed a right paw bias at all age groups. However, males had a left paw bias in puppyhood and right paw bias in older age groups. We conclude that owner handedness influences paw preference in dogs, and it should be considered when suitably pairing dogs to potential owners, especially in assistance work.

Investigating the influence of neuter status on paw preference in dogs and cats

ABSTRACT Motor lateralization is commonly observed through preferential paw use in dogs and cats. Previous studies have uncovered sex-related differences in paw preference, hypothesizing that these differences may be related to sex hormones. The current study aimed to compare neutered and entire individuals to further investigate whether paw preference is influenced by sex hormones. Dog and cat owners were required to fill in a questionnaire with demographic information such as sex and neuter status of their pets. They then carried out two simple paw preference tasks within their homes: a “reaching for food” task and a “reaching for a toy” task. This study revealed an overall preference among the 272 dogs and 137 cats tested to use their right paw in both tasks. In cats, the degree of paw preference (i.e., regardless of the direction) was significantly influenced by an interaction between neuter status and life stage. Also in dogs, both life stage and an interaction between neuter status and life stage tended to influence the degree of paw preference. Post-hoc power analysis revealed a lack of statistical power, suggesting that future studies using a larger sample size are needed to further investigate potential effects of neuter status on paw preference.

The Relationship Between the Motor Lateralization and the Concentration of Cortisol and Tyrosine in Dogs Exposed to Stress During Veterinary Activities

ABSTRACT The aim of the study was to assess the relationship between the motor lateralization in dogs and the concentration of cortisol and tyrosine in their plasma during a visit to a veterinary clinic. The research group consisted of 56 dogs. Motor lateralization was tested by of an adhesive tape test. The stress intensity was assessed basing on the levels of cortisol and ft4 in the serum blood. The statistical analysis revealed that in the group of 56 dogs there were 14 left-pawed dogs, 36 right-pawed dogs, and 6 dogs did not show particular paw preference. The average cortisol levels in particular groups were as follows: 7.94 ug/dl, 3.92 ug/dl and 3.7 ug/dl, whereas the level of tyrosine in the subjects that demonstrated left-sided lateralization (mean ± SE) (1.95 ± 0.46 ng/dl) for those with right-side lateralization (1.56 ± 0.23 ng/dl) and ambilateral dogs (1.01 ± 0.22 ng/dl). The statistical calculation of Pearson’s χ2 showed a significant relationship between the sex and the lateralization (χ2 = 6.238, df = 2, p = 0.0442).

When left is right: The effects of paw preference training on behaviour in mice

Spontaneous limb preferences exist in numerous species. To investigate the underlying mechanisms of these preferences, different methods, such as training, have been developed to shift preferences artificially. However, studies that systematically examine the effects of shifting preferences on behaviour and physiology are largely missing. Therefore, the aim of this study was to assess the impact of shifting paw preferences via training on spontaneous home cage behaviour, as well as anxiety-like behaviour and exploratory locomotion (Elevated plus maze test, Dark light test, Open field test, Free exploration test), learning performance (Labyrinth-maze) and stress hormones (fecal corticosterone metabolites) in laboratory mice (Mus musculus f. domestica). For this, we assessed spontaneous paw preferences of C57BL/6J females (Nambilateral = 23, Nleft = 23, Nright = 25). Subsequently, half of the individuals from each category were trained once a week for four weeks in a food-reaching task to use either their left or right paw, respectively, resulting in six groups: AL, AR, LL, LR, RL, RR. After training, a battery of behavioural tests was performed and spontaneous preferences were assessed again. Our results indicate that most mice were successfully trained and the effect of training was present days after training. However, a significant difference of preferences between RL and LL mice during training suggests a rather low training success of RL mice. Additionally, preferences of L mice differed from those of A and R mice after training, indicating differential long-term effects of training in these groups. Furthermore, left paw training led to higher levels of self-grooming, possibly as a displacement behaviour, and more time spent in the light compartment of the Dark light test. However, overall, there was no systematic influence of training on behavioural measures and stress hormones. Different explanations for this lack of influence, such as the link between training and hemispheric functioning or the intensity and ecological relevance of the training, are discussed.

Is There an Association between Paw Preference and Emotionality in Pet Dogs?

Simple SummarySimilar to human handedness, many animals preferentially use one limb over the other to perform certain tasks. It has been argued this handedness/limb preference could be linked to individual differences in emotionality (i.e., the tendency to appraise the environment in certain ways emotionally). A better understanding of this under-explored link has the potential to establish limb preference as an indicator of emotional vulnerability and increased risk for affective disorders (e.g., anxiety disorder, depression). This study explored a potential relationship between paw preference and emotionality in pet dogs. To determine paw preference, we examined paw preferences to hold a food-stuffed Kong™ and to lift first when starting walking or when stepping over a hurdle. Dogs’ emotionality was assessed using a validated owner-based questionnaire examining their sensitivity to positive and negative situations. In contrast to previous research this study did not find any association between dogs’ paw use and their emotionality but suggested that paw use might be task-specific. More research is needed to identify which limb is actually the preferred one in a given task and to define both the consistency and nature of any bias before drawing conclusions about their relationship with emotional appraisal of the environment.Research with humans and other animals has suggested that preferential limb use is linked to emotionality. A better understanding of this still under-explored area has the potential to establish limb preference as a marker of emotional vulnerability and risk for affective disorders. This study explored the potential relationship between paw preference and emotionality in pet dogs. We examined which paw the dogs preferentially used to hold a Kong™ and to perform two different locomotion tests. Dogs’ emotionality was assessed using a validated psychometric test (the Positive and Negative Activation Scale—PANAS). Significant positive correlations were found for dogs’ paw use between the different locomotion tasks, suggesting that dogs may show a more general paw preference that is stable across different types of locomotion. In comparison, the correlations between the Kong™ Test and locomotion tests were only partially significant, likely due to potential limitations of the Kong™ Test and/or test-specific biomechanical requirements. No significant correlations were identified between paw preference tests and PANAS scores. These results are in contrast to previous reports of an association between dog paw preference and emotionality; animal limb preference might be task-specific and have variable task-consistency, which raises methodological questions about the use of paw preference as a marker for emotional functioning.

Are wild wolves southpaws? Including potential conservation implications

Lateralization, or a left-right bias in behavior (e.g., handedness), was originally thought to exclusively exist in humans, but is now known to be widespread. Lateralization can exist at the individual or group level. In dogs (Canis lupus familiaris), tests of paw preference have produced inconsistent results. Because wolves (C. l.) differ genetically, morphologically, and behaviorally from dogs, I was interested in assessing them for lateralization. I examined lateralization (right versus left) of the foot captured (a step test analog) of wild wolves (n = 93) trapped for radiocollaring purposes in the Superior National Forest, Minnesota from 2011 – 2017 and 2019. No support was found for lateralization, and sex and age class were not significant predictors of which foot was captured. Because many mammals demonstrate lateralization, and because population-level lateralization is thought to convey increased social cohesion, it is surprising that wild wolves did not demonstrate population level lateralization. This step test analog may not have been an appropriate measure (as lateralization is task dependent) and / or wolf lateralization may exist at the individual level, but not the population level. Future work on wolf lateralization at both the individual and population levels examining pawedness via multiple tasks while accounting for potential confounding factors (such as different rearing conditions and methods) could provide clarification. Examining potential trade-offs between the costs and benefits of lateralization that these highly social animals may incur would be very interesting in terms of evolution and in comparison with dogs. Furthermore, because lateralization has been connected to emotional functioning and animal welfare, baseline lateralization data from wild wolves may inform captive wolf management and conservation, including the captive breeding programs for endangered Mexican wolves (C. l. baileyi) and red wolves (C. rufus) and other programs (e.g., educational facilities).

The effect of sex and age on paw use within a large sample of dogs (Canis familiaris)

Many studies have focused on handedness as proxy for lateralization, highlighting that population-level biases in handedness seem to be characteristic of most animals, although the strength and direction varies between species. Gender has been found to influence handedness in some species; in humans, for instance, males are more likely to be left-handed than females. Another aspect that has been comparatively unexplored is the role of age in shaping functional asymmetries, especially in adulthood.Handedness has been widely explored in dogs (Canis familiaris), although there are contrasting findings about the influence of the dogs’ sex and age, possibly due to the comparatively small sample sizes used in previous studies. Here we overcame this issue by investigating these effects in a unique sample of 17,901 dogs of unknown neutered status. Specifically, this study used pre-collected data from the British Broadcasting Corporation (BBC)’s ‘Test Your Pet’ survey, during which owners tested their dogs’ paw preference in retrieving food from a tube in 3 consecutive trials. Based on the results of these 3 trials, owners were asked to assess whether their dogs used their left or right forepaw most of the time, or if it was difficult to tell. 13,240 dogs were scored as having a paw preference (i.e., were not ambidextrous or ambiguous), representing 74.0 % of the dogs tested. In those dogs that showed a paw preference, there was a population-level right-paw preference in both male and female dogs (60.7 % female and 56.1 % male), however, the proportion of dogs with a right-paw preference was significantly higher in females than males. Elderly dogs also tended to show a stronger right-paw preference than younger dogs. We conclude that the effect of sex on handedness may be influenced by factors such as sex hormones, while age changes may reflect the development of handedness and its maintenance in dog populations.

A step in the right direction: the effect of context, strain and sex on paw preference in mice

Behavioural lateralization is a widespread phenomenon in the animal kingdom and can be an indicator of brain asymmetries in structure or function. It is often assessed using only one measure within the same study. Additionally, this measure is usually obtained in artificial situations, where the subjects undergo tasks requiring learning and forced performance of a specific action. Combining different measures of lateralization seems to be particularly promising, as artificially tested and spontaneously displayed behaviours may cover different aspects of behavioural lateralization. Therefore, the aim of the present study was to provide a comprehensive characterization of behavioural lateralization, using the laboratory mouse, Mus musculus f. domestica, as a model system. We examined lateralization using four behavioural measures, namely food reaching in an artificial test situation (the reference, most established, test) and three spontaneous behaviours (grid climbing, self-grooming and barrier crossing). We investigated their temporal consistency, assessed the association of the three spontaneous behaviours with the established food-reaching test and explored possible influences of genetic background and sex. We found differences in the distributions of lateralization across these behaviours, with fewer mice showing laterality in spontaneous behaviours. All behaviours showed high temporal consistency for the direction of laterality, while only food reaching did so for the strength of laterality. Furthermore, laterality of the spontaneous behaviours was unrelated to laterality of the food-reaching test. Lastly, strain affected behavioural lateralization, with C57BL/6J mice being more strongly lateralized than CD-1 mice for some behaviours, while sex did not affect it. Therefore, we conclude that behavioural lateralization is affected not only by genetic background, but also by the complexity of a given task. To obtain a comprehensive picture of behavioural lateralization, several measures from different contexts thus need to be included in a single study.

Effect of Stroking on Serotonin, Noradrenaline, and Cortisol Levels in the Blood of Right- and Left-Pawed Dogs.

Simple SummaryThe endocrine balance, reflected in the level of neuromodulators, is necessary for maintenance of homeostasis and facilitates adaptation to stressful conditions. An important role in processing the information reaching the brain is played by the asymmetric specialization of cerebral hemispheres referred to as laterality. The domestic dog shows preference of the forelimb determined by the predominance of the activity of the right or left brain hemisphere. Investigations of various animal species indicate that the left-brain hemisphere is involved in the control of unchanging stimuli or repetitive actions, while the right hemisphere is specialized in the quality of emotional reactions such as aggression or fear. A skillful observation of combined behavioral and physiological symptoms of stress in dogs provides better insight in the dog’s perception of veterinary care, and any means of reduction of the stress level is highly recommended, as it improves animal’s welfare. The results of the present study indicate that dogs’ laterality and sex affect the stress response and stroking can relieve stress. The level of the analyzed neuroregulators indicating intensification of stress or adaptation to stress conditions was higher in the males and in the right-pawed dogs. Our results confirm our assumptions that right-pawed dogs are better adapted to stressful conditions.It has been assumed that stroking relieves stress responses in dogs, and dogs with the activation of the left-brain hemisphere (right-pawed) may show better adaptation to stress conditions. The aim of the study was to determine whether the stroking stimulus induced changes in the level of selected neuroregulators in dogs’ blood and whether these changes depended on the sex and the predominance of the activity of one of the brain hemispheres. The study involved 40 dogs of various breeds and both sexes. The experimental animals were subjected to a behavioral tests (Kong test), and the levels of noradrenaline, serotonin, and cortisol were determined in their blood plasma. The results of the behavioral test revealed that most dogs exhibited increased activity of the left hemisphere. Furthermore, irrespective of the sex and paw preference, stroking the animal was found to alleviate the stress response, which was reflected in reduced cortisol levels and increased serotonin levels. It was found that the plasma noradrenaline, cortisol, and serotonin levels were lower in the female dogs than in the males. Additionally, the plasma noradrenaline and serotonin levels were higher in the right-pawed dogs than in the left-pawed dogs. The present results confirm the assumption that right-pawed dogs adapt to stressful conditions more readily.

Paw preference in wolves (Canis lupus): A preliminary study using manipulative tasks

ABSTRACT Behavioural and brain lateralization is widespread among non-human vertebrates. Motor lateralization has been investigated in the domestic dog, revealing that “pawedness” in this species seems to be sex and task related; however, few if any studies considered this asymmetry in wolves (Canis lupus). The aim of this study was to investigate the paw preference of seven wolves housed at Parco Natura Viva – Italy, during the interaction with food-related (FD) and olfactory (OLF) environmental enrichment devices. Eleven sessions were done (22 session in total) per condition (FD and OLF), and data about enrichment manipulation were collected. Most of the wolves manipulated the enrichment devices using one paw rather than both paws. At the individual level, all subjects were lateralized in paw use, six were right pawed, one was left pawed (the alpha male) regardless of the enrichment condition. The fact that one paw rather than both was frequently involved in manipulation could indicate a practical advantage for each individual in being lateralized in paw use. Despite the small sample size, our results provide interesting insights about lateralization in wolves, deserving further investigations. More studies are needed considering factors such as temperament, social rank and task complexity on canid motor lateralization.

Paw preference is associated with behavioural despair and spatial reference memory in male rats

Paw preference, one of the well-studied behavioural markers of asymmetry, has been associated with affective states and pathologies such as behavioural despair, a rodent model of clinical depression. However, a consistent differential effect of paw preference has not been observed for cognitive functions. In order to investigate the affective properties of paw preference together with its potential cognitive effects, we grouped male Wistar rats as left- or right-pawed, and tested them in the forced swim test and Morris water maze for behavioural despair and spatial memory performance, respectively. We found that left-pawed rats were significantly more susceptible to behavioural despair, while spatial learning performance of the two groups were not different over a five-day Morris water maze task. Left-pawed rats, however, displayed a better reference memory than the right-pawed ones on the subsequent probe trial when the hidden platform of the maze was removed. These findings indicate paw preference as a vulnerability factor for behavioural despair and reveal a previously unknown association between left-paw preference and reference memory performance as assessed in the probe trial of the Morris water maze.

The relationship between problem-solving ability and laterality in cats

The association between hemispheric asymmetries and cognitive ability is one of the key areas of comparative laterality research. In several animal species, individual limb preferences correlate with perceptual, cognitive, or motor abilities, possibly by increasing dexterity of one limb and minimizing response conflicts between hemispheres. Despite this wealth of research, the association between laterality and cognitive abilities in the cat (Felis catus) is not well understood. Therefore, it was the aim of the present study to investigate the relationship between laterality and problem-solving ability in cats. To this end, strength and direction of paw preferences in 41 cats were measured using two novel food reaching tasks in which the animals needed to open a lid in order to reach the food reward. We found that cats that showed a clear preference for one paw were able to open more lids succesfully than ambilateral animals. Moreover, cats that preferred to interact with the test apparatus with their paw from the beginning, opened more lids than cats the first tried to interact with the test apparatus using their heads. Results also suggested a predictive validity of the first paw usage for general paw usage. It was also shown that the cats’ individual paw preferences were stable and task-independent. These results yield further support to the idea that lateralization may enhance cognitive abilities.

Learning is negatively associated with strength of left/right paw preference in wild grey squirrels (Sciurus carolinensis)

Cerebral laterality, via hemispheric specialisation, has been evidenced across the animal kingdom and linked to cognitive performance in a number of species. Previously it has been suggested that cognitive processing is more efficient in brains with stronger hemispheric differences in processing, which may be the key fitness benefit driving the evolution of laterality. However, evidence supporting a positive association between cognitive performance and lateralization is mixed: data from studies of fish and birds show a positive relationship whereas more limited data from studies of mammals suggest a weak or even negative relationship, suggesting the intriguing possibility of a mammal/non-mammal divide in the nature of this relationship. Here, we report an empirical test examining the relationship between lateralization and cognitive performance in wild grey squirrels (Sciurus carolinensis) by measuring left/right paw preference as a behavioural assay of cerebral lateralization and learning speed as an assay of cognitive efficiency. We carried out a motor-based laterality test using a reaching paradigm and measured learning speed on a problem-solving task. In accordance with the suggestion of a mammal/non-mammal divide, we found a negative relationship between strength of paw preference and performance on the learning task. We discuss this finding in light of niche-specific adaptations, task-specific demands and cognitive flexibility.

Laterality as a Tool for Assessing Breed Differences in Emotional Reactivity in the Domestic Cat, Felis silvestris catus.

Simple SummaryCat breeds differ enormously in their emotional reactivity, a factor that can impact upon the success of the pet-owner relationship, with indirect consequences for animal welfare. Traditional methods of assessing emotional reactivity in cats have focused largely on questionnaire-based assessments of breed-specific behavioural profiles. We explored whether paw preferences, which have been linked to emotional reactivity in animals, are related to cat breed. The paw preferences of 4 commonly owned cat breeds were tested on a food-reaching challenge. Cats’ paw preferences differed between the breeds. Bengal cats were more likely to show a left-sided paw preference than other breeds, whilst Persians showed the weakest paw preferences, veering more heavily towards ambilaterality. Results confirmed our earlier work in showing a strong tendency for left paw use in male cats and right paw use in females. We propose that paw preference measurement could provide a useful method for assessing emotional reactivity in domestic cats, adding to our currently limited artillery of tools for determining breed-specific profiles. Such information would be of benefit to individuals considering the acquisition of a new cat, and, in the longer term may help to foster more successful cat-owner relationships, leading to indirect benefits to feline welfare.Cat breeds differ enormously in their behavioural disposition, a factor that can impact on the pet-owner relationship, with indirect consequences for animal welfare. This study examined whether lateral bias, in the form of paw preference, can be used as a tool for assessing breed differences in emotional reactivity in the cat. The paw preferences of 4 commonly owned breeds were tested using a food-reaching challenge. Cats were more likely to be paw-preferent than ambilateral. Maine Coons, Ragdolls and Bengals were more likely to be paw-preferent than ambilateral, although only the Bengals showed a consistent preference for using one paw (left) over the other. The strength of the cats’ paw use was related to cat breed, with Persians being more weakly lateralised. Direction of paw use was unrelated to feline breed, but strongly sex-related, with male cats showing a left paw preference and females displaying a right-sided bias. We propose that paw preference measurement could provide a useful method for assessing emotional reactivity in domestic cats. Such information would be of benefit to individuals considering the acquisition of a new cat, and, in the longer term, may help to foster more successful cat-owner relationships, leading to indirect benefits to feline welfare.

Functional cerebral asymmetry in dogs living under different environmental conditions

Investigating the link between stress and functional cerebral asymmetry (FCA) has been a subject of interest in recent years. The demonstration of this link in the field of veterinary medicine is particularly important as measuring FCA has the potential to be an alternative and non-invasive behavioral method to assess stress in dogs. The present study aimed to investigate whether FCA is affected by different environmental conditions in dogs.The main aim of this study was to investigate the changes in FCA in dogs living under different conditions. To this aim, strength and direction of FCA in 40 urban free-ranging dogs were measured by a Kong test. Dogs were divided into four groups considering their environmental conditions: The dogs in Group 1 (n = 8) were rehabilitated urban free ranging dogs, which were kept in enriched shelter conditions. The dogs in Group 2 (n = 9) were adopted free ranging dogs, which lived in home environment for more than 1 year. The dogs in Group 3 (n = 11) were urban free ranging dogs, which stayed in individual cages in a dog shelter for more than 6 months. The dogs in Group 4 (n = 12) were adopted urban free ranging dogs, which stayed in a dog pension for more than 30 days. Considering the length of their stay in a kennel environment, quality of living condition and emotional states, the dogs in Group 3 and 4 were classified as chronically stressed dogs.Statistically significant differences existed between groups considering strength and direction of lateralization. Most of the dogs in Group 1 (87.5 %) and Group 2 (77.8 %) showed significant paw preferences, whereas most of the dogs in the Group 3 (72.7 %) and Group 4 (75 %) were categorized as ambilateral. Considering the individual level asymmetry in dogs and environmental conditions of dogs in Group 3 and 4, one may suggest that high ambilaterality levels is related with chronic stress. Thus, reduced FCA may not be the reason for stress sensibility, rather it can be an outcome of stressful situations. These results are the first to demonstrate the possible link between chronic stress and ambilaterality in dogs.