Patterns Of Carbon Flow Research Articles

Glacial melt impacts carbon flows in an Antarctic benthic food web

Most coastal glaciers on the West Antarctic Peninsula are in retreat. Glacial ice scouring and lithogenic particle runoff increase turbidity and shape soft sediment benthic communities. This, in turn, has the potential to induce a shift in these systems from an autotrophic to a heterotrophic state. In this study, we investigated the influence of glacial runoff on carbon flows in the soft-sediment food web of Potter Cove, a well-studied shallow fjord located in the northern region of the West Antarctic Peninsula. We constructed linear inverse food web models using a dataset that includes benthic carbon stocks as well as carbon production and respiration rates. The dataset offers detailed spatial information across three locations and seasonal variations spanning three seasons, reflecting different degrees of disturbance from glacial melt runoff. In these highly resolved food web models, we quantified the carbon flows from various resource compartments (phytoplankton detritus, macroalgae, microphytobenthos, sediment detritus) to consumers (ranging from prokaryotes to various functional groups in meio- and macrofauna). Locations and seasons characterized by high glacial melt runoff exhibited distinct patterns of carbon flow compared to those with low glacial melt runoff. This difference was primarily driven by a less pronounced benthic primary production pathway, an impaired microbial loop and a lower secondary production of the dominant bivalve Aequiyoldia eightsii and other infauna in the location close to the glacier. In contrast, the bivalve Laternula elliptica and meiofauna had the highest secondary production close to the glacier, where they are exposed to high glacial melt runoff. This study shows how the effects of glacial melt propagate from lower to higher trophic levels, thereby affecting the transfer of energy in the ecosystem.

Inter-Provincial Power Transmission and Its Embodied Carbon Flow in China: Uneven Green Energy Transition Road to East and West

Inter-provincial power transmission in China solved the problem of electricity production and consumption spatial mismatch, which also facilitated Chinese green energy transition and sustainable development. Aiming to understand the spatial patterns and main driving factors, this paper accounted the aggregate carbon intensity per electricity produced by each province, built the inter-provincial Out–Destination matrixes of carbon emission embodied in power transmission among 27 provincial areas of 2006 and 2019, and quantitatively analyzed the change in spatial patterns of carbon flow and carbon intensity difference between power inward and outward provinces. We found that the inter-provincial power transmission has led to the reduction in the national average carbon intensity per electricity produced from 0.855 kg/kWh in 2006 to 0.628 kg/kWh in 2019 and the green energy transition of all provinces. The spatial morphology characteristics of carbon flow show the ‘three corridors of West-East Power Transmission (WEPT)’ pattern, in which power is transmitted from some main thermal power-dominated provinces in central and north-west China to the eastern coastal provinces. Further, it resulted in the carbon leakage from electricity consumption of the coastal provinces and carbon overload of the hinterland provinces. Despite natural resource endowment, the location of the provinces in the national power grid, the national low-carbon energy transition strategy, as well as advances in long-distance ultra-high-voltage power transmission technologies were the main factors to the formation and evolution of Chinese electricity green transition.

Microbial community composition controls carbon flux across litter types in early phase of litter decomposition.

SummaryLeaf litter decomposition is a major carbon input to soil, making it a target for increasing soil carbon storage through microbiome engineering. We expand upon previous findings to show with multiple leaf litter types that microbial composition can drive variation in carbon flow from litter decomposition and specific microbial community features are associated with synonymous patterns of carbon flow among litter types. Although plant litter type selects for different decomposer communities, within a litter type, microbial composition drives variation in the quantity of dissolved organic carbon (DOC) measured at the end of the decomposition period. Bacterial richness was negatively correlated with DOC quantity, supporting our hypothesis that across multiple litter types there are common microbial traits linked to carbon flow patterns. Variation in DOC abundance (i.e. high versus low DOC) driven by microbial composition is tentatively due to differences in bacterial metabolism of labile compounds, rather than catabolism of non‐labile substrates such as lignin. The temporal asynchrony of metabolic processes across litter types may be a substantial impediment to discovering more microbial features common to synonymous patterns of carbon flow among litters. Overall, our findings support the concept that carbon flow may be programmed by manipulating microbial community composition.

Temporal patterns of carbon flow from grassland vegetation to soil microorganisms measured using 13C-labelling and signature fatty acids

PurposeWe investigated how the C flow from plants to microorganisms varies throughout the year in a temperate grassland. Additionally, we investigated how the C flow relates to saprotrophic activity and vegetation changes.MethodsIn situ stable isotope pulse labelling (13CO2) was employed to estimate the flow of recently plant-derived C to soil microorganisms by using signature fatty acids. Bacterial and fungal growth was estimated using radio-labelling in laboratory incubations.ResultsThe C flow from plants to arbuscular mycorrhizal (AM) fungi peaked during the warmer parts of the year, but saprotrophic microorganisms showed little temporal variation in C flow. Also saprotrophic fungi received considerable amounts of C from plants throughout the year. Bacterial and fungal growth showed temporal variation with a growth peak in August for both. This suggests a shift in the C source from mainly rhizosphere C in colder parts of the year, to older C-sources in warmer parts of the year (August).ConclusionWe conclude that AM fungi, saprotrophic fungi and bacteria differ in the amount of recently-fixed C they receive from plants throughout the year. Hence, temporal patterns need to be considered to understand ecosystem functioning. The studied plant community included winter annuals, which potentially maintain a high C flow to saprotrophic fungi during the cold season.

Carbon endowment and trade-embodied carbon emissions in global value chains: Evidence from China

Recent literature highlights global value chains and the trade-embodied carbon emissions; however, it fails to explain how changes in positions within global value chains affect the emissions embodied in a certain economy’s trade. Carbon emissions are the by-product of production activities that use fossil-fuel-based energy; these emissions can be treated as an ideal measure of the carbon endowment of a certain economy, and they are directly related to environmental regulations. This study constructs an extended environmental Heckscher-Ohlin-Vanek model by treating carbon emissions as a measure of carbon endowments to explain the flow patterns of the trade-embodied emissions under a global-value-chain framework. This study also investigates the impact of a certain economy’s emissions embodied in trade due to a change in position within a global value chain. The results show that a Heckscher-Ohlin-Vanek model can be used to explain the carbon-flow patterns of China and its trading partners. Economies gathered at the two ends of these global value chains are found to have lower or even negative net carbon outflows, while economies that are trapped at the middle-to-high levels of their global value chains, especially those with relatively abundant carbon endowments, tend to have higher net carbon outflows. To reduce their net emissions embodied in trade, these economies should institute stricter environmental regulations, optimize their energy structures and improve their energy efficiencies. Another effective way to eliminate the carbon-lock-in effect would be move up or down along global value chains to stay close to either end of the production chain.

Stable Isotope Analysis Reveals the Importance of Riparian Resources as Carbon Subsidies for Fish Species in the Daning River, a Tributary of the Three Gorges Reservoir, China

The patterns of carbon flow in large rivers influenced by dams are still not well known. We hypothesized that spatial variation in fish assimilation of basal production sources would be affected by heterogeneity of landscape-scale hydrology within a watershed due to the impoundment. We used stable isotope analysis and Bayesian mixing models to estimate relative contributions of basal production sources to fish consumers in a tributary of the Three Gorges Reservoir (TGR) with remarkably heterogeneous habitats between the upper and lower reaches. Model estimates indicated that riparian C3 plants were the main basal food source irrespective of reaches or feeding groups. Autochthonous algae carbon also appeared to be important as secondary carbon sources. However, the major contributive aquatic algae in the upstream were benthic algae while planktonic algae were important in the downstream, and this difference was related to their distinct hydrological characteristics. The contribution from C4 plants was comparatively less important to all fish consumers. Our results highlighted the significance of the riparian C3 carbon source to fish consumers in the TGR tributary and that the difference in landscape-scale characteristics was not enough to influence the pattern, which may be attributed to abundant standing stock of the riparian plants as well as hydrologic regimes in this river.

Stable isotope analysis suggests the existence of multiple populations of streaked spinefoot (Siganus javus L.) in Bandon Bay, Southern Thailand

The stock unit used in fisheries resource assessment and management is generally based on the morphological and genetic characteristics of a particular population or species to avert problems caused by the treatment of multiple populations as one stock, which can lead to the overestimation of population sizes and genetic pollution. Furthermore, since the linkage of microhabitats is an important factor affecting the reproduction of marine organisms in coastal areas, an understanding of the food web in each microhabitat is essential to establish sustainable fisheries management practices. We investigated spatial variations in the food sources and feeding habits of immature stage of Siganus javus using genetic population analyses and stable isotope analyses (δ13C and δ15N). These species are commonly harvested by small-scale fisheries, and it inhabits Bandon Bay in the Surat Thani Province of Southern Thailand. Genetic variation within sampling sites was greater than that between sites. The δ13C values of S. javus differed between sites, which suggest that the different ecological habitats exhibit different rates and patterns of carbon flow even among sites located in the same bay. Our results suggest that studies combining genetic population analyses and stable isotope analyses are required to confirm the delineation of fine-scale management units intended for the development of coastal fishery resources.

Impact of short-term warming on seasonal variations in bacterial growth, grazing, and viral lysis in coastal waters of Taiwan

Although temperature is a key parameter controlling the activity and growth of all microorganisms, information about how water temperature may affect the trophic interactions and carbon flow patterns of microbial food webs is not consistent. We investigated the response in bacterial gross growth, grazing rates, and viral lysis to small temperature changes (3°C above in situ values) in coastal waters of Taiwan using a modified dilution method approach over a 1 yr period from September 2013 to September 2014. Warming increased the bacterial gross growth rates and bacterial losses to grazing, demonstrating clear seasonality. The warming conditions led to a 5 to 200% increase in bacterial gross growth rates. The increase ratios of bacterial gross growth rates were low (5 to 25%) at higher ambient temperatures (>25°C) but increased exponentially at lower ambient temperatures (<25°C) with warming. Grazing increased in parallel with seasonal bacterial growth, while viral lysis did not. These results could prove useful in forming a testable hypothesis about the possible directions of change of microbial carbon fluxes that may accompany the warming of the coastal waters of Taiwan.

Impact of warming on phyto‐bacterioplankton coupling and bacterial community composition in experimental mesocosms

Global warming is assumed to alter the trophic interactions and carbon flow patterns of aquatic food webs. The impact of temperature on phyto-bacterioplankton coupling and bacterial community composition (BCC) was the focus of the present study, in which an indoor mesocosm experiment with natural plankton communities from the western Baltic Sea was conducted. A 6 °C increase in water temperature resulted, as predicted, in tighter coupling between the diatom-dominated phytoplankton and heterotrophic bacteria, accompanied by a strong increase in carbon flow into bacterioplankton during the phytoplankton bloom phase. Suppressed bacterial development at cold in situ temperatures probably reflected lowered bacterial production and grazing by protists, as the latter were less affected by low temperatures. BCC was strongly influenced by the phytoplankton bloom stage and to a lesser extent by temperature. Under both temperature regimes, Gammaproteobacteria clearly dominated during the phytoplankton peak, with Glaciecola sp. as the single most abundant taxon. However, warming induced the appearance of additional bacterial taxa belonging to Betaproteobacteria and Bacteroidetes. Our results show that warming during an early phytoplankton bloom causes a shift towards a more heterotrophic system, with the appearance of new bacterial taxa suggesting a potential for utilization of a broader substrate spectrum.

Spatial and temporal patterns of carbon flow in a temperate, large river food web

Using natural abundances of stable carbon (δ13C) and nitrogen (δ15N) isotopes, we quantified spatial and temporal patterns of carbon flow through the main channel food web in the lowland section of New Zealand’s longest river, the Waikato River. The study was undertaken with the objective of determining whether the Waikato River conforms to contemporary theoretical concepts regarding carbon flow in large river food webs. Potential organic carbon sources and invertebrate and fish consumers were sampled from three different hydrogeomorphic zones on six occasions, representing a range of seasonal and flow conditions. In line with the predictions of the riverine productivity model and riverine ecosystem synthesis, autochthonous algae and biofilms were the most important basal carbon source contributing to consumer biomass. These were often supported by C3 aquatic macrophytes and allochthonous C3 riparian plants. The relative importance of organic carbon sources differed between zones and appeared to change depending on season, presumably in response to water temperature and flow, particularly in the unconstrained zone of the lower river. We also demonstrate that to draw robust conclusions, consideration must be given to quantifying the isotopic signatures of organisms lower in the food web, as these can change significantly between sampling times and hydrogeomorphic zones.

Stable carbon isotopic compositions of intact polar lipids reveal complex carbon flow patterns among hydrocarbon degrading microbial communities at the Chapopote asphalt volcano

Seepage of asphalt forms the basis of a cold seep system at 3000 m water depth at the Chapopote Knoll in the southern Gulf of Mexico. Anaerobic microbial communities are stimulated in the oil-impregnated sediments as evidenced by the presence of intact polar membrane lipids (IPLs) derived from archaea and Bacteria at depths up to 7 m below the seafloor. Detailed investigation of stable carbon isotope composition (δ 13C) of alkyl and acyl moieties derived from a range of IPL precursors with distinct polar head groups resolved the complexity of carbon metabolisms and utilization of diverse carbon sources by uncultured microbial communities. In surface sediments most of the polar lipid-derived fatty acids with phosphatidylethanolamine (PE), phosphatidylglycerol (PG) and diphosphatidylglycerol (DPG) head groups could be tentatively assigned to autotrophic sulfate-reducing bacteria, with a relatively small proportion involved in the anaerobic oxidation of methane. Derivatives of phosphatidyl-( N)-methylethanolamine (PME) were abundant and could be predominantly assigned to heterotrophic oil-degrading bacteria. Archaeal IPLs with phosphate-based hydroxyarchaeols and diglycosidic glyceroldibiphytanylglyceroltetraethers (GDGTs) were assigned to methanotrophic archaea of the ANME-2 and ANME-1 cluster, respectively, whereas δ 13C values of phosphate-based archaeols and mixed phosphate-based and diglycosidic GDGTs point to methanogenic archaea. At a 7 m deep sulfate–methane transition zone that is linked to the upward movement of gas-laden petroleum, a distinct increase in abundance of archaeal IPLs such as phosphate-based hydroxyarchaeols and diglycosidic archaeol and GDGTs is observed; their δ 13C values are consistent with their origin from both methanotrophic and methanogenic archaea. This study reveals previously hidden, highly complex patterns in the carbon-flow of versatile microbial communities involved in the degradation of heavy oil including hydrocarbon gases that would not have been evident from classical compound-specific isotope analyses of either bulk IPL or apolar lipid derivatives.

Landscape-Scale Hydrologic Characteristics Differentiate Patterns of Carbon Flow in Large-River Food Webs

Efforts to conserve, restore, or otherwise manage large rivers and the services they provide are hindered by limited understanding of the functional dynamics of these systems. This shortcoming is especially evident with regard to trophic structure and energy flow. We used natural abundances of carbon and nitrogen isotopes to examine patterns of material flow in ten large-river food webs characterized by different landscape-scale hydrologic characteristics (low-gradient river, high-gradient river, river stretches downstream of reservoirs, and reservoirs), and tested predictions from three ecosystem concepts commonly applied to large-rivers: The River Continuum Concept, The Flood Pulse Concept and the Riverine Productivity Model. Carbon derived from aquatic C3 plants and phytoplankton were the dominant energy sources supporting secondary consumers across the ten large-river food webs examined, but relative contributions differed significantly among landscape types. For low-gradient river food webs, aquatic C3 plants were the principal carbon source, contributing as much as 80% of carbon assimilated by top consumers, with phytoplankton secondarily important. The estimated relative importance of phytoplankton was greatest for food webs of reservoirs and river stretches downriver from impoundments, although aquatic C3 plants contributed similar amounts in both landscape types. Highest 99th percentile source contribution estimates for C4 plants and filamentous algae (both approximately 40%) were observed for high-gradient river food webs. Our results for low-gradient rivers supported predictions of the Flood Pulse Concept, whereas results for the three other landscape types supported the Riverine Productivity Model to varying degrees. Incorporation of landscape-scale hydrologic or geomorphic characteristics, such as river slope or floodplain width, may promote integration of fluvial ecosystem concepts. Expanding these models to include hydrologically impacted landscapes should lead to a more holistic understanding of ecosystem processes in large-river systems.

Simulated patterns of carbon flow in the pelagic food web of Lake Fryxell, Antarctica: Little evidence of top-down control

A model was developed to explore patterns of carbon flow through the pelagic food web of Lake Fryxell, Taylor Valley, Antarctica. The goals of this study were to quantify patterns of carbon flow and test hypotheses of top-down versus bottom-up controls on this system. The model included seven trophic groups: bacteria, photosynthetic nanoflagellates (PNAN), heterotrophic nanoflagellates (HNAN), ciliates (bacterial feeding and flagellate feeding), phytoplankton and rotifers (the top predator); all inputs were driven by predatory demands. This system has no insects, vertebrates, crustacea or allochthonous inputs from terrestrial plants. Autotrophs and bacteria contributed ca. 94% of total community biomass, with all other heterotrophic organisms representing <6% of the total. We defined a measure of trophic efficiency ( λ) as the ratio of carbon uptake by rotifers to total carbon uptake by all trophic groups. λ was always <2% of total community carbon flow and seldom correlated to other flows of carbon through the system. Sensitivity analysis revealed that λ was relatively insensitive to efficiency of carbon transfers between other components of the food web. In total, these results suggest little top-down influence of the top predator on system dynamics. Conversely, major fluctuations in total community biomass followed general patterns of seasonal and inter-annual availability of PAR, suggesting bottom-up control on this system.

Effect of inorganic nutrients on relative contributions of fungi and bacteria to carbon flow from submerged decomposing leaf litter.

The relative contributions of fungi and bacteria to carbon flow from submerged decaying plant litter at different levels of inorganic nutrients (N and P) were studied. We estimated leaf mass loss, fungal and bacterial biomass and production, and microbial respiration and constructed partial carbon budgets for red maple leaf disks precolonized in a stream and then incubated in laboratory microcosms at two levels of nutrients. Patterns of carbon flow for leaf disks colonized with the full microbial assemblage were compared with those colonized by bacteria but in which fungi were greatly reduced by placing leaf disks in colonization chambers sealed with membrane filters to exclude aquatic hyphomycete conidia but not bacterial cells. On leaves colonized by the full microbial assemblage, elevated nutrient concentrations stimulated fungi and bacteria to a similar degree. Peak fungal and bacterial biomass increased by factors of 3.9 and 4.0; cumulative production was 3.9 and 5.1 times higher in the high nutrient in comparison with the low nutrient treatment, respectively. Fungi dominated the total microbial biomass (98.4 to 99.8%) and cumulative production (97.3 and 96.5%), and the fungal yield coefficient exceeded that of bacteria by a factor of 36 and 27 in low- and high-nutrient treatments, respectively. Consequently, the dominant role of fungi in leaf decomposition did not change as a result of nutrient manipulation. Carbon budgets indicated that 8% of leaf carbon loss in the low-nutrient treatment and 17% in the high-nutrient treatment were channeled to microbial (essentially fungal) production. Nutrient enrichment had a positive effect on rate of leaf decomposition only in microcosms with full microbial assemblages. In treatments where fungal colonization was reduced, cumulative bacterial production did not change significantly at either nutrient level and leaf decomposition rate was negatively affected (high nutrients), suggesting that bacterial participation in carbon flow from decaying leaf litter is low regardless of the presence of fungi and nutrient availability. Moreover, 1.5 and 2.3 times higher yield coefficients of bacteria in the reduced fungal treatments at low and high nutrients, respectively (percentage of leaf carbon loss channeled to bacterial production), suggest that bacteria are subjected to strong competition with fungi for resources available in leaf litter.

Carbon flow patterns in the planktonic food web of the Gulf of Riga, the Baltic Sea: a reconstruction by the inverse method

We used the inverse method to reconstruct a carbon flux model for the planktonic food web of the southern part of the Gulf of Riga, the Baltic Sea. The model was based on data from three field campaigns (3–12 May 1995; 28 June–7 July 1994; 26 August–3 September 1993). The carbon flow model indicated very different channelling of gross primary production (GPP) in the three different periods. In spring 55% of GPP was channelled to detritus, while in summer 35% was exudated and channelled to dissolved organic carbon (DOC). In autumn, 42% of GPP was channelled to the zooplankton compartments. The model suggested high bacterial respiration rates and low bacterial growth efficiencies (6%) in spring and autumn. During these periods large decreases in DOC were anticipated to occur due to bacterial respiration. In summer the model suggested that bacterial respiration rate was low and that the bacterial growth efficiency was high (60%). During this period a net accumulation of DOC apparently occurred. Respiration was the most important loss of organic carbon in all seasons, corresponding on average to 152, 59 and 391% of GPP daily in spring, summer and autumn, respectively. Daily sedimentation rates of particulate organic carbon (POC) were consistently low (<3% d −1), removing 13–29% of GPP daily. The sedimented material consisted mainly of amorphous detritus. This indicates that in spite of the relatively high level of eutrophication, the planktonic system in the Gulf of Riga has a high short-term carbon retention and recycling capacity, and little POC is lost from the upper water column through sedimentation.

Carbon allocation in wild-type and Glc+ Rhodobacter sphaeroides under photoheterotrophic conditions.

The photosynthetic bacterium Rhodobacter sphaeroides is capable of producing H2 via nitrogenase when grown photoheterotrophically in the absence of N2. By using 14C-labeled malate, it was found that greater than 95% of this substrate was catabolized completely to CO2 during H2 production. About 60% of this catabolism was associated with H2 biosynthesis, while almost 40% provided reductant for other cellular purposes. Thus, only a small fraction of malate provided carbon skeletons. The addition of ammonium, which inhibited nitrogenase activity, increased substrate conversion into carbon skeletons threefold. Catabolism of malate occurred primarily via the tricarboxylic acid cycle, but gluconeogenesis was also observed. The wild-type organism grew poorly on glucose, accumulated gluconate and 2-keto-3-deoxygluconate, and did not produce H2. More than 50% of metabolized glucose appeared in carbon skeletons or in storage compounds. A glucose-utilizing mutant was five times more effective in utilizing this substrate. This mutant produced H2 from glucose, using 74% of metabolized substrate for this purpose. Glucose converted to storage products or to other carbon skeletons was reduced to 8%. Fixation of CO2 competed directly with H2 production for reducing equivalents and ATP. Refixation of CO2 released from these substrates under H2-producing conditions was, at most, 10 to 12%. Addition of ammonium increased refixation of respired CO2 to 83%. Patterns of carbon flow of fixation products were associated with the particular strains and culture conditions.

Patterns of carbon flow from glucose to methane in a thermophilic anaerobic bioreactor

[U- 14C]Glucose, added carrier-free to sludge from a thermophilic anaerobic bioreactor being fed a lignocellulose waste, was rapidly turned over with less than one-third of the original radiolabel remaining as glucose after 5 s of incubation. The primary labeled products found were [ 14C]acetate and 14CO 2, which were in a ratio near 2:1. Further incubation resulted in the disappearance of [ 14C]acetate and the appearance of an equivalent amount of label as 14CH 4 and 14CO 2. No significant production of [ 14C]propionate, butyrate, lactate, or ethanol was detected from [ 14C]glucose, even if these potential intermediates (unlabeled) were added to the sludge at a concentration of 1 mM to trap any label entering their pools. Addition of 0.8 atm (80 kPa) of H 2 to the headspace over sludge resulted in some accumulation of [ 14C]lactate and a corresponding decrease in [ 14C]acetate produced from [ 14C]glucose. Production of [ 14C]propionate, butyrate and ethanol were still not significant in the presence of H 2. Incubation of sludge for 1 h in the presence of hydrogen resulted in increases in the lactate and formate concentrations, but not those of propionate, butyrate, or ethanol. These results demonstrate that glucose was metabolized directly to acetate, CO 2, and H 2 by the microbial populations in the bioreactor with little carbon from glucose flowing through other intermediates, indicating a high degree of coupling between glucose fermentation and hydrogen uptake. The short-term response of these microbial populations to elevated H 2 partial pressures was to increase lactate production.

Patterns of carbon flow from glucose to methane in a thermophilic anaerobic bioreactor

[U-14C]Glucose, added carrier-free to sludge from a thermophilic anaerobic bioreactor being fed a lignocellulose waste, was rapidly turned over with less than one-third of the original radiolabel remaining as glucose after 5 s of incubation. The primary labeled products found were [14C]acetate and 14CO2, which were in a ratio near 2:1. Further incubation resulted in the disappearance of [14C]acetate and the appearance of an equivalent amount of label as 14CH4 and 14CO2. No significant production of [14C]propionate, butyrate, lactate, or ethanol was detected from [14C]glucose, even if these potential intermediates (unlabeled) were added to the sludge at a concentration of 1 mM to trap any label entering their pools. Addition of 0.8 atm (80 kPa) of H2 to the headspace over sludge resulted in some accumulation of [14C]lactate and a corresponding decrease in [14C]acetate produced from [14C]glucose. Production of [14C]propionate, butyrate and ethanol were still not significant in the presence of H2. Incubation of sludge for 1 h in the presence of hydrogen resulted in increases in the lactate and formate concentrations, but not those of propionate, butyrate, or ethanol. These results demonstrate that glucose was metabolized directly to acetate, CO2, and H2 by the microbial populations in the bioreactor with little carbon from glucose flowing through other intermediates, indicating a high degree of coupling between glucose fermentation and hydrogen uptake. The short-term response of these microbial populations to elevated H2 partial pressures was to increase lactate production.

Assimilation of carbon by the soil biomass

The stoichiometry of soil biomass synthesis was studied with glucose, glucose+N, alfalfa and corn leaf amended soil. Equations describing the quantitative relationship found for the dissimilation of substrate C and related oxidative assimilation of C by the biomass were developed. The analytical approach involved measurement of soluble C consumed by persulfate oxidation (POC). Carbon flow patterns showed that the observed decrease in POC correlated well with an increase in cell C (0–44 h). With glucose alone 47% of the initial POC was consumed and with glucose+N 99% of the POC was utilized. Alfalfa and corn leaf residues also showed sharp decreases in initial POC. Marked differences in C uptake were noted depending on the soil treatment and specific incubation period. An equation was developed which predicts C assimilation level and related soil biomass weights from decreases in POC.

Carbon Flux through a Coral-Reef Ecosystem: a Conceptual Model

The potential application of modeling techniques to sedimentological studies of modern carbonate environments is demonstrated by a suggested conceptual model of the carbon system in a coral-reef ecosystem. A graphic model is employed to delineate the locations and characteristics of carbon flow patterns in the reef ecosystem. Modeling is thought to be a valid tool of geological research which should be considered a necessary first step in studies of systems such as the one described. The given model is intended to stimulate discussion about the usefulness of modeling in geological investigations of coral reefs, particularly in identifying the geological priorities in coral-reef research. This model will also form the basis for future simulation analyses.