Information about the population structure, including the age and sex composition, growth characteristics, and diet of fish, is essential for the conservation and sustainable exploitation of fish stock. The burbot, Lota lota, is the only freshwater species of the family Lotidae in the Amur (Heilongjiang) River catchment located in northeastern China. Information on the biological characteristics and data on the population structure of this fish from the Amur River are scarce. To study these factors in burbot, Lota lota, in the Amur River of China, 749 specimens from four sampling areas were taken and analyzed in October 2022, January 2023, and May 2023. The ages of the sampled fish ranged from 1+ to 7+ years. The female/male ratio was 1.04:1, and body length and mass varied from 175 to 595 mm and 73.5 to 1958.7 g, respectively. The length–weight regression parameter b value was estimated as 2.80. The parameters of the von Bertalanffy growth model were L∞ = 596, K = 0.221, and t0 = −0.771 for all sampled fish; L∞ = 625, K = 0.208, and t0 = −0.756 for females; and L∞ = 584, K = 0.219, and t0 = −0.980 for males. The analysis of the stomach contents showed fish to be the major source of nutrition across all areas and sampling periods. In January, the secondary prey of burbots in the Huma reach of the upper Amur River was aquatic insect larvae, while, in the Tongjiang and Fuyuan reaches of the middle Amur River, the secondary prey was shrimp. The mean stomach fullness index and rate of empty stomachs differed with the sampling area, with the greatest proportion of empty stomachs observed in the Luobei reach of the middle Amur River, and the lowest in the Tongjiang and Fuyuan reaches. The mean stomach fullness index showed the opposite trend. In the Huma reach, the contributions of fish to the diet and the mean stomach fullness index were significantly higher in May than in October and January. The study provides the first detailed information on the population age and sex structure, growth patterns, and feeding ecology of burbots from the Amur River, China. The results will aid in formulating management strategies and regulations for local populations of burbots in the Amur River, China.