Ecosystems supply goods and services to humans and are the basis for sustainable development of human society. The study of the supply of ecosystem services and the demand and consumption of ecosystem services by human society, and the analysis of the supply and demand characteristics and flow relationships of ecosystem service flows are of great significance for the management of regional ecosystems and the development of ecological compensation. Taking the Yangtze River Economic Belt as an example, this paper calculates the supply and demand indices of ecosystem services in 2015 and 2020, and determines the ecosystem spatial flow paths and flow volumes from the ecosystem supply area to the demand area based on various methods and models such as the minimum cumulative resistance (MCR) model and distance decay model. The results indicate that 1). In 2015 and 2020, the supply and demand of ecosystem services in the Yangtze River Economic Zone show an increasing trend numerically, and there is spatial heterogeneity in the spatial distribution. In terms of ecosystem service supply per unit area, the midstream region is higher than the upstream and downstream regions. In terms of the demand for ecosystem services per unit area, the downstream is higher than the midstream and upstream. 2). From the supply-demand balance of ecosystem services in the Yangtze River Economic Zone, the midstream region is mainly the area of surplus supply of ecosystem services, and the downstream region is mainly the area of deficit supply. From 2015 to 2020, the number of areas with balanced supply and demand of ecosystem services in the Yangtze River Economic Belt decreases and the number of areas with unbalanced supply and demand increases, which is related to the changes in the level of economic development and land use patterns. 3). The flow of ecosystem services in the Yangtze River Economic Belt shows an increasing trend, from 726.59 billion yuan in 2015 to 1,450.54 billion yuan in 2020, with Jiangxi Province being the main ecosystem service supply area and Zhejiang Province being the main ecosystem service demand area in the Yangtze River Economic Belt.
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