s / Photodiagnosis and Photodynamic Therapy 12 (2015) 325–375 357 Application of photodynamic therapy in reduction in vitro of pathogenic fungus Sporothrix schenckii Gunther M.P. Guirado1,2, Ricardo S. Navarro1,2, Rodnei Rossoni1,2, Juliana C. Junqueira1,2, Luciano S. Feitosa1,2 1 Biomedical Engineering Institute, UNICASTELO, Sao Jose dos Campos, Brazil 2 School of Dentistry, University Estadual Paulista – UNESP Sao Jose dos Campos, Brazil The sporotrichosis is a disease affected by lymphatic vessels, skin and some internal organs. There is higherprevalenceof chronic mycosis from pathogenic fungus Sporothrix schenckii in tropical regions. The aim of this in vitro study was to evaluate the susceptibility of S. schenckii to effects of photodynamic therapy (PDT). In the methodology, standardized viable cells (0.1ml) (106 cells/ml) were separated into groups (n=5): G1 – low power laser (660nm) (35mW, 10 J, 285 s, 0.38 cm2 spot size, 26.3 J/cm2) and photosensitizer (0.1mg/ml methylene blue) (L+P+), G2 – laser and no photosensitizer (L+P−), G3 – no laser and photosensitizer (L−P+), G4 – no laser and no photosensitizer (L−P−). After microbiological laboratorial processing, it was performed the count of the colony forming units (CFU). The datawere analyzed by KruskallWallis statistical test. The results showed medium (minimum–maximum) values (log CFU/ml) to G1 (0)(0–0)a, G2 (3.21)(3.16–3.23)b, G3 (3.22)(3.14–3.26)b, G4 (3.24)(3.12–3.30)b, demonstrated that G1 showed no growth and statistically higher effects of PDT than other groups (p 0.05). The parameters of this study can conclude the effective and viable of PDT in inactivation of this microorganism. http://dx.doi.org/10.1016/j.pdpdt.2015.07.126 Photodynamic therapy inhibits the antimicrobial effects of bacteriophage therapy in a invertebrate model of systemic infection C.P. Sabino1, P.R. Neves2, N. Lincopan2,3, M.S. Ribeiro1 1 Center for Lasers and Applications, IPEN-CNEN/SP, Sao Paulo, Brazil 2 Department of Microbiology, Institute of Biomedical Sciences, Universidade de Sao Paulo, Sao Paulo, Brazil 3 Department of Clinical Analysis, School of Pharmacy, Universidade de Sao Paulo, Sao Paulo,