Parvovirus In Dogs Research Articles

Design of Primers for the Detection of Parvovirus in Dogs and Cats Using the Same Polymerase Chain Reaction

Design of Primers for the Detection of Parvovirus in Dogs and Cats Using the Same Polymerase Chain Reaction

Molecular, pathological and hematobiochemical detection of canine parvovirus in dogs

Molecular, pathological and hematobiochemical detection of canine parvovirus in dogs

Risk Factors of Parvovirus in Dogs at De’ Lab Pet Clinic, Bekasi

This study aimed to determine the percentage of parvovirus, risk factor analysis, and describe the symptoms and treatment of parvovirus. The study was conducted by surveying, collecting secondary data from patient medical records and Bekasi City’s rainfall data, data recording, data unification, and data analysis. Data analysis was presented descriptively and tested using Chi-Square and Odds Ratio (OR). The results shows the percentage of parvovirus was 2%. The rainfall factor <150mm/month significant to the risk of parvovirus (p<0,05) and increase the risk of parvovirus 0,154 times. The age factors 0-6 month significant to the risk of parvovirus (p<0,05) and increase the risk of parvovirus 0,017 times. The breed factors not significant to the risk of parvovirus (p>0,05) and don’t increase the risk of parvovirus. The main symptoms seen in parvovirus include vomiting, diarrhea especially bloody diarrhea, and fever. The main components treatments are fluid therapy, antibiotic, antiemetic, and nutritional support. The administration of other drugs can be given to dogs whose administration must be adjusted to their respective conditions.

Isolation and Genetic Characterization of Parvoviruses From Dogs, Cats, Minks, and Raccoon Dogs in the Eastern Region of Shandong Province, China.

The eastern region of Shandong province, China, is an intensive economic mink and raccoon dog breeding area. To investigate the molecular variations of parvovirus in cats, dogs, minks, and raccoon dogs from this region, feline panleukopenia virus (FPV), canine parvovirus 2 (CPV-2), mink enteritis virus (MEV), and raccoon dog parvovirus (RDPV) were separately isolated and characterized from the respective animals with gastroenteritis. PCR amplification showed that there were 15/18 (83.3%), 9/13 (69.2%), 8/11 (72.7%), and 3/7 (42.9%) samples from the diseased animals separately positive for FPV, CPV-2, MEV, and RDPV. Of these, a total of six FPV, six MEV, four CPV-2, and three RDPV strains were successfully isolated using F81 cells. Next, the near-complete genomes of 19 parvovirus isolates were amplified and analyzed. The viral particle 2 (VP2) sequence alignment showed that they shared 97.2–100% nucleotide similarity. Phylogenetic analysis showed that the five FPV isolates were in the same branch, and an FPV isolate was closely related with MEV and RDPV isolates obtained in this study. These suggested that cross-species infection occurred in the Shandong region between the FPV, MEV, and RDPV. For the four CPV-2 isolates, three were antigenic variant strains CPV-2a, and the other was antigenic variant strain CPV-2c. Additionally, the mutations that had emerged in the VP2 amino acids of CPV-2 also occurred in the VP2 from the FPV, MEV, and RDPV isolates. This study suggested that the continuous evolution of the parvovirus may be accelerated in areas with a high density of economic animal trading/breeding, and controlling parvovirus infection in these animals remains a challenge.

Self-assembled raccoon dog parvovirus VP2 protein confers immunity against RDPV disease in raccoon dogs: in vitro and in vivo studies

BackgroundRaccoon dog parvovirus (RDPV) causes acute infectious diseases in raccoon dogs and may cause death in severe cases. The current treatment strategy relies on the extensive usage of classical inactivated vaccine which is marred by large doses, short immunization cycles and safety concerns.MethodsThe present study aimed at optimization of RDPV VP2 gene, subcloning the gene into plasmid pET30a, and its subsequent transfer to Escherichia coli with trigger factor 16 for co-expression. The protein thus expressed was purified with ammonium sulfate precipitation, hydrophobic chromatography, and endotoxin extraction procedures. VLPs were examined by transmission electron microscopy, dynamic light scattering, and the efficacy of VLPs vaccine was tested in vivo.ResultsResults indicated that RDPV VP2 protein could be expressed soluble. Transmission electron microscopy and dynamic light scattering results indicated that RDPV VP2 self-assembled into VLPs. Hemagglutination inhibition antibody titers elicited by Al(OH)3 adjuvanted RDPV VLPs were comparable with RDPV inactivated vaccines, and the viral loads in the blood of the struck raccoon dogs were greatly reduced. Hematoxylin and eosin and Immunohistochemical results indicated that RDPV VLPs vaccine could protect raccoon dogs against RDPV infections.ConclusionsThese results suggest that RDPV VLPs can become a potential vaccine candidate for RDPV therapy.

Small animal disease surveillance 2020/21: SARS-CoV-2, syndromic surveillance and an outbreak of acute vomiting in UK dogs.

Small animal disease surveillance 2020/21: SARS-CoV-2, syndromic surveillance and an outbreak of acute vomiting in UK dogs.

Comparison of Four Commercially Available Point-of-Care Tests to Detect Antibodies against Canine Parvovirus in Dogs.

Measuring antibodies to evaluate dogs’ immunity against canine parvovirus (CPV) is useful to avoid unnecessary re-vaccinations. The study aimed to evaluate the quality and practicability of four point-of-care (POC) tests for detection of anti-CPV antibodies. The sera of 198 client-owned and 43 specific pathogen-free (SPF) dogs were included; virus neutralization was the reference method. Specificity, sensitivity, positive and negative predictive value (PPV and NPV), and overall accuracy (OA) were calculated. Specificity was considered to be the most important indicator for POC test performance. Differences between specificity and sensitivity of POC tests in the sera of all dogs were determined by McNemar, agreement by Cohen’s kappa. Prevalence of anti-CPV antibodies in all dogs was 80% (192/241); in the subgroup of client-owned dogs, it was 97% (192/198); and in the subgroup of SPF dogs, it was 0% (0/43). FASTest® and CanTiCheck® were easiest to perform. Specificity was highest in the CanTiCheck® (overall dogs, 98%; client-owned dogs, 83%; SPF dogs, 100%) and the TiterCHEK® (overall dogs, 96%; client-owned dogs, 67%; SPF dogs, 100%); no significant differences in specificity were observed between the ImmunoComb®, the TiterCHEK®, and the CanTiCheck®. Sensitivity was highest in the FASTest® (overall dogs, 95%; client-owned dogs, 95%) and the CanTiCheck® (overall dogs, 80%; client-owned dogs, 80%); sensitivity of the FASTest® was significantly higher compared to the one of the other three tests (McNemars p-value in each comparison: <0.001). CanTiCheck® would be the POC test of choice when considering specificity and practicability. However, differences in the number of false positive results between CanTiCheck®, TiterCHEK®, and ImmunoComb® were minimal.

When the diagnosis of parvovirus in dogs and cats becomes challenging.

Parvoviruses (PV) can cause outbreaks with high morbidity and mortality in dogs and cats. Even if typical cases exist in puppies and kittens, PV infection (PVI) can have many different clinical presentations, making the laboratory support necessary. The aim of this work was to evaluate retrospectively the frequency of misdiagnoses, particularly missed diagnoses, of PVI in 144 suspected cases (88 clinical cases and 56 necropsies) involving 96 dogs and 48 cats. A nested PCR test was chosen as the gold standard. An index of diagnostic suspicion (IDS) for PVI, based on parameters reported upon submittal of the samples, was introduced to classify the initial diagnoses issued by veterinarians. The agreement between the IDS of PVI and PCR results was calculated. The effect of species, age and clinical versus necroscopic presentation was evaluated by logistic regression. In 63.6% of the cases, the IDS was confirmed by the PCR, whereas in 36.4% there was a missed diagnosis or a diagnosis wrongly attributed to PVI. More accurate results were obtained for dogs, animals aged < 1 year, and necropsies. Parvovirus infection should be better investigated in patients with atypical or few clinical signs, in particular in cats and animals over 1 year old.

Assessment of intestinal and cardiac-related biomarkers in dogs with parvoviral enteritis.

The aim of this study was to evaluate the intestinal and cardiac biomarkers in the determination of intestinal and cardiac damage in dogs with parvoviral enteritis. The material of this study consisted of 10 healthy dogs (control group) and 30 dogs with parvoviral enteritis (experimental group) admitted to the Department of Internal Medicine, Faculty of Veterinary Medicine, Selcuk University.Serum samples were extracted from the collected blood samples taken from vena cephalicavenipuncture for analysis of blood gases, haemogram and to measure the levels of intestinal-fatty acid-binding protein (I-FABP), trefoil factor 3 (TFF-3), claudin-3 (CLDN-3), heart-type fatty acid-binding protein (H-FABP), cardiac troponin I (cTnI), and creatine kinase-myocardial band (CK-MB) by enzyme linked immunosorbent assay (ELISA) test kits. Statistically significant decreases in the blood gas hydrogen ion concentration (pH), partial pressure of oxygen (pO2), sodium (Na), bicarbonate (HCO3), and oxygen saturation (SatO2) levels and significant increase in the levels of I-FABP, TFF-3, CK-MB, cTnI and also in the haemogram, a decrease in leukocyte (WBC) level and an increase in platelet (THR) level were detected in parvoviral dogs compared to the control group (p⟨0.05). Also ROC analysis revealed on 0th hour for the utility of I-FABP and on 48th hour for TFF-3 in differentiating in the experimental group between the survivor and non-survivor dogs. Other intestinal-related biomarker (CLDN-3) and none of the cardiac-related biomarkers (H-FABP, CK-MB and cTnI) are not high enough for prediction of mortality.In conclusion, it was determined that I-FABP and TFF-3 for the intestinal injury and morta-lity prediction, and CK-MB and cTnI for the cardiac injury were useful and reliable biomarkers to determine the damage caused by parvovirus in dogs.

Detection of Canine Parvovirus in Dogs by Means Polymerase Chain Reaction

Worldwide, the infection with canine parvovirus type 2 (CPV-2) is one of the main causes of hemorrhagic enteritis in dogs and having a diagnostic technique that is highly sensitive is essential for veterinarians, dog owners and breeders. In this work, a protocol was implemented that uses the conventional Polymerase Chain Reaction (PCR) to detect a CPV-2 DNA fragment from feces of dogs with clinical signology corresponding to canine parvovirus. In total, 12 stool samples that were positive with conventional PCR were collected and analyzed, which was confirmed by sequencing the fragments obtained and contrasted with the sequences of the different variants of CPV-2 described in the GenBank database.

Study of adjuvant effect of fosprenil on immunogenicity of vaccines against parvovirus enteritis and rabies in experiment

To prevent most common viral diseases of small pets, vaccines are most often used that stimulate animal immune system and provide specific protection. Data on the adjuvant properties of well-known and widely used immunomodulator of natural origin, fosprenil, used in veterinary practice as an immunomodulator with antiviral activity for the correction of the immune response in various viral infections, during experimental immunization of BALB/mouse vaccines against dog parvovirus enteritis and rabies are presented in the article. It was shown that fosprenil use in animals at immunostimulating dose (5 pg/mouse) significantly enhances the effect of immunization with both vaccines. So, fosprenil significantly increases the titer of post-vaccination antibodies to standard liquid inactivated vaccine against parvovirus enteritis in dogs. In experiments with the rabies vaccine, it was found that the administration of fosprenil in animals along with vaccine not only contributes to significant increase in the titer of specific antibodies, but also to significant increase in coefficient of protective activity of rabies vaccine. Thus, fosprenil exerts an adjuvant effect on post-vaccination immunity caused by vaccination of mice with rabies and parvovirus enteritis vaccines.

New variants of canine parvovirus in dogs in southern Brazil.

Carnivore protoparvovirus 1 (canine parvovirus 2, CPV-2) has undergone a rapid evolution through mutations in the capsid protein VP2, giving rise to variants associated with unique clinicopathological and immunological features. VP2 is a major capsid protein involved in key steps of virus biology, including interactions with cellular receptors and with the immune system. This study analyzed the complete VP2 coding sequence of 38 CPV-2 isolates obtained from dogs with clinical parvovirosis in southern Brazil. Amplicons encompassing the whole VP2 coding region were subjected to nucleotide sequencing, and predicted amino acid sequences were analyzed to identify molecular markers of viral variants. Viral variants were classified as CPV-2a, -2b or -2c based on the presence of the amino acid Asn, Asp or Glu, respectively, at VP2 residue 426. Amino acid sequence analysis identified 20 CPV-2c and four CPV-2b isolates. Eleven viruses were identified as New CPV-2a, two as New CPV-2b, and one resembled the original CPV-2 and was designated CPV-2-like. In addition to the mutation at amino acid 426 of VP2, new 2a/2b variants containing a Ser297Ala mutation at residue 297 were identified. CPV-2-like samples contained some mutations that were also present in the original CPV-2 isolate, including as Leu, Thr, Ala and Asp at residues 87, 101, 300 and 305, respectively. The New CPV-2a isolates had three additional mutations (Phe267Tyr, Tyr324Ile and Thr440Ala) associated with selective pressure and development of disease in vaccinated dogs. The resemblance of the CPV-2-like isolate to CPV-2 suggests reemergence of CPV-2 and/or evolution from vaccine strains. Phylogenetic analysis grouped the variants with their respective reference strains, in general, according to amino acid changes. These results demonstrate the high VP2 diversity of CPV circulating in dogs in southern Brazil and indicate the emergence of new viral variants that differ markedly from the current vaccine strains.

Аналіз штамів парвовірусу собак на основі послідовностей VP-2 гену

One of the most dangerous diseases of dogs is canine parvovirus enteritis. The causative agent of enteritis is Canine Parvovirus type 2 (CPV-2) of the subgroup Feline panleukopenia virus (FPV) of the species Carnivore protoparvovirus 1 of the family Parvoviridae. For prevention both in the world and in Ukraine vaccination is used. Despite this measure, parvovirus enteritis still causes death of dogs with a clinical and pathological pattern characteristic of the disease. The sequence of dog parvovirus strains was analyzed, which are presented in the GenBank database: No. MH213140.1, KP881653.1, GU212792.1, GU212790.1, FJ011098.1, GU212791.1, MG264079.1, GU392239.1, KF539801.1, MH491947.1, MH491855.1, DQ025952.1, MH329287.1, AY262281.1, KY921606.1. Among the given strains, 5 (GU212792.1, GU212790.1, FJ011098.1, GU212791.1, KY921606.1) are used for the manufacture of vaccines registered in Ukraine. Pathogenic isolates were isolated in Italy, Hungary, France, Taiwan, China, South Korea, etc. As a result of the studies, SNPs (Single Nucleotide Polymorphism) were revealed in the sequences and studied, and a phylogenetic tree was constructed (method of attachment of neighbors). The smallest distances were established between the vaccine strains VAC_M primodog (GU212790.1), VAC_P vanguard (GU212791.1), VAC_C quantum (GU212792.1). Intervet vaccine strains (FJ011098.1) and MX-VACVBC / 17 (KY921606.1) are in a separate cluster from the other three vaccine strains. They form a separate cluster. Also, the closest distances are established between the isolates according to the territorial distribution. The results obtained may indicate differences in the antigenic structure of circulating pathogenic strains of dog parvovirus. The lack of data from epizootological monitoring of the disease using molecular genetic methods in Ukraine shows the need for such studies.

Detección de parvovirus canino por PCR y su asociación con algunos de los factores de riesgo

Objetivo. El parvovirus canino es una de las enfermedades más importantes en perros en todo el mundo la cual amenaza su salud. El objetivo de este estudio fue determinar la prevalencia del parvovirus canino en perros remitidos a la Clínica de Pequeños Animales de la Universidad de Teherán en la provincia de Teherán por el método de detección del genoma. Materiales y Métodos. Se obtuvieron muestras de heces diarreicas de 60 perros durante los años 2014-2016 y se investigó la presencia de CPV por el método de PCR. Resultados. La prevalencia de infección por parvovirus canino en los perros estudiados fue del 8,33% (5 0f 60). No se encontraron diferencias significativas entre los diferentes grupos de edad y razas. Sin embargo, la infección fue significativamente mayor en perros con diarrea hemorrágica (3 de 25), pero no fue estadísticamente significativa usando la prueba de chi-cuadrada (P&gt;0,05). Conclusiones. Este estudio demostró que la diarrea hemorrágica y la falta de vacunación pueden considerarse como síntomas y factores de riesgo importantes de la infección por parvovirus canino en perros, y que los propietarios de perros en Irán deben ser conscientes del hecho de que la vacunación contra la infección CPV debe hacerse con cuidado y a tiempo para controlar y prevenir el virus.

Identification of co-infection by rotavirus and parvovirus in dogs with gastroenteritis in Mexico.

This is the first report on circulating canine rotavirus in Mexico. Fifty samples from dogs with gastroenteritis were analyzed used polymerase chain reaction and reverse transcription polymerase chain reaction in order to identify parvovirus and rotavirus, respectively; 7% of dogs were infected with rotavirus exclusively, while 14% were co-infected with both rotavirus and parvovirus; clinical signs in co-infected dogs were more severe.

Prevalence of serum antibody titers against canine distemper virus and canine parvovirus in dogs hospitalized in an intensive care unit.

OBJECTIVE To determine the prevalence of dogs hospitalized in an intensive care unit (ICU) with serum antibody titers against canine distemper virus (CDV) and canine parvovirus (CPV). DESIGN Prospective observational study. ANIMALS 80 dogs. PROCEDURES Dogs hospitalized in an ICU for > 12 hours between February 1 and June 1, 2015, that had at least 0.25 mL of serum left over from diagnostic testing were eligible for study inclusion. Dogs with serum antibody titers > 1:32 (as determined by serum neutralization) and > 1:80 (as determined by hemagglutination inhibition) were considered seropositive for CDV and CPV, respectively. The date of last vaccination was obtained from the medical record of each dog. RESULTS Of the 80 dogs, 40 (50%) and 65 (81%) dogs were seropositive for CDV and CPV, respectively. Of the 40 dogs that were seronegative for CDV, 27 had been vaccinated against CDV within 3 years prior to testing. Of the 15 dogs that were seronegative for CPV, 3 had been vaccinated against CPV within 3 years prior to testing. Ten dogs were seronegative for both CDV and CPV. CONCLUSIONS AND CLINICAL RELEVANCE Results indicated the prevalence of dogs hospitalized in an ICU that were seropositive for CDV and CPV was lower than expected given the high vaccination rate reported for dogs. Although the antibody titer necessary to prevent disease caused by CDV or CPV in critically ill dogs is unknown, adherence to infectious disease control guidelines is warranted when CDV- or CPV-infected dogs are treated in an ICU.

Full-length genomic and molecular characterization of Canine parvovirus in dogs from North of Brazil.

With the objective of characterizing Canine parvovirus (CPV) from some suspected fecal samples of dogs collected from the Veterinarian Hospital in Belém city, five positive samples were found by PCR assay and an update molecular characterization was provided of the CPV-2 circulation in Belém. Through sequencing of the complete DNA sequences (NS1, NS2, VP1, and VP2 genes), the CPV-2 strain was identified as CPV-2b (Asn426Asp) circulating in Belém. The CPV-2b strain with a different change at the position Tyr324Leu was detected in all samples assessed and thus reported for the first time for the scientific community. Phylogenetic analysis indicated that Belém CPV-2b and CPV-2a strains would be related to a cluster with samples after the 1990s, suggesting that CPV-2b in Belém originated from CPV-2a circulating in Brazil after the 1990s. Potential recombination events were analyzed using RDP4 and SplitsTree4; therefore, results suggest that CPV-2 sequences here described were not potentially recombination events. Continuous monitoring and molecular characterization of CPV-2 samples are needed not only to identify possible genetic and antigenic changes that may interfere with the effectiveness of vaccines but also to bring a better understanding of the mechanisms that drive the evolution of CPV-2 in Brazil.

Seroprevalence of Canine Parvovirus in Dogs in Lusaka District, Zambia.

Canine parvovirus (CPV) enteritis is a highly contagious enteric disease of young dogs. Limited studies have been done in Zambia to investigate the prevalence of CPV in dogs. Blood was collected from dogs from three veterinary clinics (clinic samples, n = 174) and one township of Lusaka (field samples, n = 56). Each dog's age, sex, breed, and vaccination status were recorded. A haemagglutination assay using pig erythrocytes and modified live parvovirus vaccine as the antigen was used. Antibodies to CPV were detected in 100% of dogs (unvaccinated or vaccinated). The titres ranged from 160 to 10240 with a median of 1280. Vaccinated dogs had significantly higher antibody titres compared to unvaccinated (p < 0.001). There was a significant difference in titres of clinic samples compared to field samples (p < 0.0001) but not within breed (p = 0.098) or sex (p = 0.572). Multiple regression analysis showed that only age and vaccination status were significant predictors of antibody titres. The presence of antibody in all dogs suggests that the CPV infection is ubiquitous and the disease is endemic, hence the need for research to determine the protection conferred by vaccination and natural exposure to the virus under local conditions.

Occurrence of canine parvovirus in dogs from Henan province of China in 2009-2014.

BackgroundThere is no information concerning the genotype of Canine parvovirus (CPV) currently circulating in Henan province, China. Therefore, the aim of the present study was to provide insights into the epidemiology and molecular characterization of CPV circulating in Henan province from 2009 to 2014.ResultsNineteen thousand nine hundred seven dogs from pet hospitals in the cities of Luoyang, Anyang, Jiaozuo, Sanmenxia, Xinxiang, Zhengzhou in Henan province between 2009 and 2014 were investigated. Over the 6-year period, 1169 CPV-positive cases were identified and the morbidity of CPV infection ranged from 4.16 to 8.06 %, although morbidity was not significant (P > 0.05) between 2009 and 2014. Factors associated with morbidity included sampling season, dog age, breed, vaccination status, and sex. CPV co-infection with coccidium (10.00 %), canine distemper virus (4.79 %), hookworm (2.40 %), canine coronavirus (1.11 %), roundworm (1.03 %), tapeworm (0.17 %) and Babesia spp. (0.09 %) were observed. The new CPV-2a variant was more prevalent than the new CPV-2b variant in Henan province. CPV 2c was not observed in this study.ConclusionsThe epidemiology of CPV infection and identification of the circulating genotypes in Henan province, China from 2009 to 2014 determined that the new CPV-2a variant was more prevalent.

Factors affecting the occurrence of canine parvovirus in dogs

Canine parvovirus (CPV) is the most important enteric virus infecting canids worldwide. The purpose of this study was to detect CPV in naturally infected dogs from several veterinary clinics distributed throughout Portugal between 2012 and 2014 and to identify risk factors associated with CPV infection. From 209 dogs suspected of being infected with CPV, historical data and clinical signs were collected. Fecal samples were screened for CPV by PCR assay and those positive were confirmed by sequencing. The data was analyzed using logistic regression to investigate associations between each of the predisposing factors and CPV status. Of the samples collected, 77.5% tested CPV-positive. Statistical analysis showed that animals in the three age categories (p<0.001) were at list 12 times more likely to be CPV-positive than older animals. The anthelminthic treatment [OR=0.45, p=0.04] and the rectal temperature (hypothermia, [OR=0.12, p=0.004]) contributed to decrease the likelihood of the dogs be infected with CPV. On the other hand, clinical signs such as depression [OR=4.4, p=0.02] and dehydration status [OR=2.38, p=0.001] made dogs more likely to be CPV-infected. The results indicate that although having a high morbidity, only 18% of the Portuguese dog population died in the study. Some of the risk factors identified in this study have not been commonly reported, yet they are easy to obtain and can be used as prognostic indicators in the veterinary practice.