Partial CCA Research Articles

Estimating the Causal Effect from Partially Observed Time Series

Many real-world systems involve interacting time series. The ability to detect causal dependencies between system components from observed time series of their outputs is essential for understanding system behavior. The quantification of causal influences between time series is based on the definition of some causality measure. Partial Canonical Correlation Analysis (Partial CCA) and its extensions are examples of methods used for robustly estimating the causal relationships between two multidimensional time series even when the time series are short. These methods assume that the input data are complete and have no missing values. However, real-world data often contain missing values. It is therefore crucial to estimate the causality measure robustly even when the input time series is incomplete. Treating this problem as a semi-supervised learning problem, we propose a novel semi-supervised extension of probabilistic Partial CCA called semi-Bayesian Partial CCA. Our method exploits the information in samples with missing values to prevent the overfitting of parameter estimation even when there are few complete samples. Experiments based on synthesized and real data demonstrate the ability of the proposed method to estimate causal relationships more correctly than existing methods when the data contain missing values, the dimensionality is large, and the number of samples is small.

Temperature transfer functions based on freshwater testate amoebae from China

Globally, lakes and reservoirs ecosystems are integral parts of ecological processes. Nevertheless, global warming is rapidly changing their function and sustainability, especially in the populated area of the northern hemisphere. Here we examined testate amoebae community and 10 environmental variables from 51 lakes and reservoirs across China and developed testate amoebae transfer functions for temperature based on both abundance- and biomass-datasets. A total of 169 testate amoebae taxa were identified. Our partial CCA analyses revealed that water temperature explained 5.15% (P = 0.006) and 5.57% (P = 0.008) of the total variance in testate amoebae abundance and biomass, respectively. The WA-PLS was the best model in abundance-based temperature transfer function (RMSEP = 2.87 °С, R2 = 0.60), whereas the MAT proved to be the best model for biomass-based temperature transfer function (RMSEP = 3.34 °С, R2 = 0.67). The application of all models should be carried out with suitable precautions. Our results suggested that freshwater testate amoebae could contribute to a better understanding of the ecological integrity and its vulnerability in inland aquatic environments.

Epilithic diatom assemblages and their relationships with environmental variables in the Nilüfer Stream Basin, Bursa, Turkey.

Patterns of epilithic diatom species distribution in relation to environmental variables from 12 sampling sites on the main stream and some of its tributaries in the Nilüfer Stream Basin were determined using multivariate statistical techniques. The stream basin has been heavily influenced by anthropogenic effects. The upper part of the basin that is distant from pollution sources mostly has a spring water quality, while the lower part where the stream flows through the urban area and receives domestic and industrial wastewater has a quite low quality. Ordination techniques using both diatom taxa and 21 environmental variables revealed non- to slightly polluted upper basin sites and highly polluted lower basin sites along the stream. The results showed that the stream catchment is polluted gradually from upstream to downstream and that most of the downstream sites have very low water quality especially in summer months. A total of 134 epilithic diatom taxa belonging to 50 genera were recorded for 12 sample sites. Partial CCA results indicated that water temperature (T), discharge (Q), and total phosphorus (TP) were the most important variables affecting the distribution of diatom species. Unpolluted or slightly polluted upper basin sites were dominated by Achnanthidium minutissimum, Cocconeis placentula var. euglypta, Gomphonema olivaceum, and Navicula tripunctata. Highly polluted lower basin sites were characterized by high levels of organic and inorganic matters and low dissolved oxygen (DO) values. Species widespread in the highly polluted lower basin sites such as Nitzschia umbonata, Nitzschia amphibia, Nitzschia capitellata, Nitzschia palea, Nitzschia paleacea, Luticola mutica, and Stephanodiscus niagarae were mostly related to pollution indicator variables such as ammonium nitrogen (NH4+-N), sodium (Na+), total phosphorus (TP), and total organic matter (TOM).

Herb layer in silver fir – beech forests in the western Pyrenees: Does management affect species diversity?

Silver fir-beech forests form ecologically important ecosystems in European mountains, where they have been intensively managed due to timber harvesting. Forest management effects can be evaluated using herb layer diversity as an indicator of environmental changes. Therefore, revealing the patterns of herb layer diversity is necessary for developing strategies for sustainable management. Knowledge on management history of fir-beech forests in the western Pyrenees is still limited. In this study we aim to disentangle the environmental drivers of species richness and species composition of these forests emphasizing the role of forest management. We sampled the herb layer vascular plants in 68 plots distributed among 14 silver fir-beech stands of the association Scillo lilio-hyacinthi-Fagetum sylvaticae and related diversity patterns with management histories, light conditions and topographic and climatic variables. Four management categories were established: recently managed, long managed, recently abandoned and long abandoned. Species richness was analysed by GLMM and species composition by partial CCA and PCNM. Multivariate dispersion analysis was used to assess differences in beta diversity among management categories. Management, continentality and slope had in general a weak effect on species richness, continentality with a positive effect whilst slope and management, in category of recently abandoned stands, with a negative effect. In addition, the percentage of large gaps had a moderate effect on open area species richness. Species composition was related to slope, ombrothermic index, forest management and spatial effects in terms of neutral processes. Long managed forests showed differences in beta diversity with recently abandoned and long abandoned forests. Our study suggests that environmental variables in combination with spatial effects are the main drivers of species diversity at small sampling scale, highlighting that management plays an important role in these western Pyrenean silver fir-beech forests.

Variation partitioning of diatom species data matrices: Understanding the influence of multiple factors on benthic diatom communities in tropical streams

Elucidating the confounding influence of multiple environmental factors on benthic diatom communities is important in developing water quality predictive models for better guidance of stream management efforts. The objective of this study was to explore the relative impact of metal pollution and hydromorphological alterations in, addition to nutrient enrichment and organic pollution, on diatom taxonomic composition with the view to improve stream diatom-based water quality inference models. Samples were collected twice at 20 sampling stations in the tropical Manyame Catchment, Zimbabwe. Diatom, macroinvertebrate communities and environmental factors were sampled and analysed. The variations in diatom community composition explained by different categories of environmental factors were analysed using canonical correspondence analysis using variance partitioning (partial CCA). The following variations were explained by the different predictor matrices: nutrient levels and organic pollution - 10.4%, metal pollution - 8.3% and hydromorphological factors - 7.9%. Thus, factors other than nutrient levels and organic pollution explain additional significant variation in these diatom communities. Development of diatom-based stream water quality inference models that incorporate metal pollution and hydromorphological alterations, where these are key issues, is thus deemed necessary.

Influence of environmental variables on stream fish fauna at multiple spatial scales

ABSTRACT Effects of environmental variables at different spatial scales on freshwater fish assemblages are relatively unexplored in Neotropical ecosystems. However, those influences are important for developing management strategies to conserve fish diversity and water resources. We evaluated the influences of site- (in-stream) and catchment-scale (land use and cover) environmental variables on the abundance and occurrence of fish species in streams of the Upper Araguari River basin through use of variance partitioning with partial CCA. We sampled 38 1st to 3rd order stream sites in September 2009. We quantified site variables to calculate 11 physical habitat metrics and mapped catchment land use/cover. Site and catchment variables explained > 50% of the total variation in fish species. Site variables (fish abundance: 25.31%; occurrence: 24.51%) explained slightly more variation in fish species than catchment land use/cover (abundance: 22.69%; occurrence: 18.90%), indicating that factors at both scales are important. Because anthropogenic pressures at site and catchment scales both affect stream fish in the Upper Araguari River basin, both must be considered jointly to apply conservation strategies in an efficient manner.

Spatial variability of chromophoric dissolved organic matter in a large floodplain river: control factors and relations with phytoplankton during a low water period

AbstractChromophoric dissolved organic matter (CDOM) affects ecological processes in freshwater environments. Few studies assessed its spatial variability and relations with phytoplankton in floodplain rivers. Therefore, these topics were examined in a hydrological connectivity gradient in the Middle Paraná system. Absorption coefficients at 250 and 365 nm were measured to estimate CDOM concentration and molecular weight (MW), to find their explanatory limnological variables (Redundancy Analysis, RDA), and to assess their contribution to the explanation of phytoplankton structure (Canonical Correspondence Analysis, CCA). Conductivity and turbidity explained CDOM significantly. Increased conductivity from the main channel to floodplain lakes was associated with increased CDOM concentration. The lake indirectly connected to the river showed the highest turbidity associated with high‐MW‐CDOM, while the isolated lake showed the highest conductivity associated with low‐MW‐CDOM. Phytoplankton structure was significantly explained by conductivity, turbidity, and high‐MW‐CDOM (CCA). Environments directly connected to the river (with the lowest conductivity) were associated with diatoms. Phytoflagellates were associated with turbidity and high‐MW‐CDOM in the lake indirectly connected to the river, whereas Cyanobacteria were associated with conductivity and low‐MW‐CDOM in the isolated lake. The combined effect of physical factors and CDOM explained the highest fraction of species variation (partial CCA). As a conclusion: (1) CDOM increases as hydrological connectivity with the river decreases; (2) lake sediment resuspension is linked to an increase of high‐MW‐CDOM; (3) lake isolation from the river corresponds to an increase in low‐MW‐CDOM; and (4) CDOM explains phytoplankton structure significantly and should be considered as an important variable in studies of microalgal assembly. Copyright © 2015 John Wiley & Sons, Ltd.

Community structure and elevational diversity patterns of soil Acidobacteria

Acidobacteria is one of the most dominant and abundant phyla in soil, and was believed to have a wide range of metabolic and genetic functions. Relatively little is known about its community structure and elevational diversity patterns. We selected four elevation gradients from 1000 to 2800m with typical vegetation types of the northern slope of Shennongjia Mountain in central China. The vegetation types were evergreen broadleaved forest, deciduous broadleaved forest, coniferous forest and sub-alpine shrubs. We analyzed the soil acidobacterial community composition, elevational patterns and the relationship between Acidobacteria subdivisions and soil enzyme activities by using the 16S rRNA meta-sequencing technique and multivariate statistical analysis. The result found that 19 known subdivisions as well as an unclassified phylotype were presented in these forest sites, and Subdivision 6 has the highest number of detectable operational taxonomic units (OTUs). A significant single peak distribution pattern (P<0.05) between the OTU number and the elevation was observed. The Jaccard and Bray–Curtis index analysis showed that the soil Acidobacteria compositional similarity significantly decreased (P<0.01) with the increase in elevation distance. Mantel test analysis showed the most of the soil Acidobacteria subdivisions had the significant relationship (P<0.01) with different soil enzymes. Therefore, soil Acidobacteria may be involved in different ecosystem functions in global elemental cycles. Partial Mantel tests and CCA analysis showed that soil pH, soil temperature and plant diversity may be the key factors in shaping the soil Acidobacterial community structure.

Do we need soil moisture measurements in the vegetation–environment studies in wetlands?

AbstractQuestionsWater level parameters are normally used as explanatory variables in ecological studies, but soil moisture may be more causally connected with species composition. Can measurements of volumetric soil moisture in vertical profiles using electromagnetic sensors improve vegetation–environment analyses in fens and bogs? Are there inter‐habitat differences in water level–moisture relationships that could explain different sensitivities of particular vegetation types to water level changes?LocationCalcareous fens, poor fen grasslands, poor fens and ombrotrophic bogs in the Czech Republic.MethodsMonitoring of soil moisture (Profile Probe PR2), water level, pH and electrical conductivity of the water; measurements of soil pH and organic carbon; vascular plant and bryophyte data sampling; Pearson's correlations; ANOVA with post‐hoc tests; PCA of environmental factors; CCA and partial CCA that controlled for the effects of pH, conductivity, and year and region of sampling; Monte Carlo permutation tests.ResultsThe median volumetric soil moisture correlated with the log‐transformed organic carbon concentration. This correlation was particularly strong when all vegetation types were merged or when poor fens and poor fen grasslands were analysed separately. The latter vegetation types further displayed the strongest correlations between water level and uppermost soil moisture. Calcareous fens had the highest water level and the lowest correlation between water level and soil moisture. In bog profiles the moisture continuously increased downwards and its variability over time correlated with water level fluctuation less strongly than in the case of Sphagnum fens. The median water level explained most variation in species data in all cases, except for separately analysed poor fen grasslands, whose variation was best explained by soil organic carbon. Moisture characteristics became insignificant whenever water level was included into the forward selection model.ConclusionsIn fens and bogs, water level still describes hydrological conditions better than directly measured moisture, and serves as a useful proxy of complex environmental conditions related to the hydrological regime, especially in Sphagnum fens with a shallow peat layer. Our results validate existing knowledge of species–environment relationships based on water table measurements.

Plant community composition and disturbance in Caspian Fagus orientalis forests: which are the main driving factors?

The oriental beech forests along the southern Caspian Sea shores of Iran are characterized by high biodiversity and high ecological value. However, there is little information concerning factors governing community composition and the presence and abundance of invasive plants. In the present study we aimed to evaluate the main drivers of community composition, diversity, and establishment of invasive plants in disturbed and undisturbed Fagus orientalis communities. We sampled 104 vegetation releves in undisturbed and disturbed sites of the Kheiroud Investigation Forest in northern Iran and collected data on a large number of environmental variables concerning soil properties, light conditions, and topography. Data analysis consisted of a forward selection of significant explanatory variables followed by partial CCA analyses. Additionally, we carried out indicator species analysis of groups of releves affected by different disturbance types and analyzed effects of disturbance on species richness, diversity, and evenness using general linear models. CCA results showed that disturbance, soil properties, light conditions and elevation explained 22.6% of the total variation in floristic composition. Gap and gap + were the most important disturbance types affecting community composition. Disturbance had significant but rather weak effects on species richness, evenness, and the Shannon index. The most obvious effect of disturbance was on species composition, as revealed by significant groups of indicator species for different disturbance types. Most of the indicator species in grazed sites were unpalatable, poisonous, and creeping species, which are avoided by cattle. Grazing led to decreased abundance of indicator species and species characteristic of old-growth Fagus forests. The most intensive disturbance type harvesting + gap + grazing was associated with invasion by native vascular plant species not typically found in beech forests (apophytes).

A survey of green plant tRNA 3'-end processing enzyme tRNase Zs, homologs of the candidate prostate cancer susceptibility protein ELAC2

BackgroundtRNase Z removes the 3'-trailer sequences from precursor tRNAs, which is an essential step preceding the addition of the CCA sequence. tRNase Z exists in the short (tRNase ZS) and long (tRNase ZL) forms. Based on the sequence characteristics, they can be divided into two major types: bacterial-type tRNase ZS and eukaryotic-type tRNase ZL, and one minor type, Thermotoga maritima (TM)-type tRNase ZS. The number of tRNase Zs is highly variable, with the largest number being identified experimentally in the flowering plant Arabidopsis thaliana. It is unknown whether multiple tRNase Zs found in A. thaliana is common to the plant kingdom. Also unknown is the extent of sequence and structural conservation among tRNase Zs from the plant kingdom.ResultsWe report the identification and analysis of candidate tRNase Zs in 27 fully sequenced genomes of green plants, the great majority of which are flowering plants. It appears that green plants contain multiple distinct tRNase Zs predicted to reside in different subcellular compartments. Furthermore, while the bacterial-type tRNase ZSs are present only in basal land plants and green algae, the TM-type tRNase ZSs are widespread in green plants. The protein sequences of the TM-type tRNase ZSs identified in green plants are similar to those of the bacterial-type tRNase ZSs but have distinct features, including the TM-type flexible arm, the variant catalytic HEAT and HST motifs, and a lack of the PxKxRN motif involved in CCA anti-determination (inhibition of tRNase Z activity by CCA), which prevents tRNase Z cleavage of mature tRNAs. Examination of flowering plant chloroplast tRNA genes reveals that many of these genes encode partial CCA sequences. Based on our results and previous studies, we predict that the plant TM-type tRNase ZSs may not recognize the CCA sequence as an anti-determinant.ConclusionsOur findings substantially expand the current repertoire of the TM-type tRNase ZSs and hint at the possibility that these proteins may have been selected for their ability to process chloroplast pre-tRNAs with whole or partial CCA sequences. Our results also support the coevolution of tRNase Zs and tRNA 3'-trailer sequences in plants.

Vegetation databases as a tool to analyse factors affecting the range expansion of the forest understory herb Ceratocapnos claviculata

AbstractQuestion: The eu‐atlantic forest herb Ceratocapnos claviculata showed a recent increase in frequency within its native range and an eastward and northward range expansion in Central Europe. To gain deeper understanding of factors affecting the range expansion of the species, we analyzed vegetation relevés at three different scales and asked the following questions: As the species occurs in a wide range of vegetation types, is variation in community composition across the entire range related to climatic environmental zones and tree cover? Are there differences in species composition and habitat characteristics between the native range and the two invaded regions (S Sweden and NE Germany)? Did community composition and habitat characteristics within the native range (The Netherlands) change between ‘before 1970’ and ‘1990 to 2006’?Location: W, C and N Europe.Methods: We analysed vegetation‐plot data with C. claviculata from various databases and own surveys using partial CCA, partial DCA, Indicator Species Analysis, MANCOVA and multiple regression.Results: Using vegetation plots from the entire distribution range, climatic environmental zones explained 68.9% of the total canonical Eigenvalue. Differences in floristic composition and habitat characteristics between the two invaded regions were as large as between native and invaded range sites. However, relevés from the invaded range were generally characterized by anthropogenic disturbance. Accordingly, abundance of C. claviculata was positively linked to silvicultural intensity. New relevés from 1990 to 2006 were characterized by higher Ellenberg nutrient indicator values, lower species diversity, higher proportions of neophytic and hemerobic species and showed a lower cover of the study species than old relevés from before 1970.Conclusions: Across the range of C. claviculata, climatic environmental zones determine vegetation composition. Accordingly, the species is characterized by a broad macroclimatic amplitude. Vegetation composition and structure differ significantly between the two regions of the new range. Thus, the species has successfully established under various biotic and abiotic environmental conditions. Beyond potential positive effects of soil eutrophication and mild winters, anthropogenic impact may directly facilitate seed dispersal and provide sites and resource conditions suitable for germination and establishment of C. claviculata, whereas a decrease of forest management may constrain the species.

Land Snail Faunas in Polish Forests: Patterns of Richness and Composition in a Post-Glacial Landscape

We examine variation in species richness, species composition and distance decay in similarity in forest snail faunas from Poland and a small part of Transcarpathian Ukraine, and their connection with geographical position in relation to Pleistocene refugia. Forest faunas were sampled from sites of standard size in each of ten regions. Data were analysed using DCA and partial CCA, extracting the geographical, climatic and ecological correlates with the major axes. Relationships among site and regional faunas were further examined through the Simpson Index of Similarity. Site species richness shows no significant variation with geographical position or climate, but relates to soil and vegetation characteristics. Composition varies with location; southern highland faunas differ from one another far more than do northern lowland faunas, showing a clear east-to-west pattern of change. The aggregate highland fauna is richer than that of the lowlands, which is a subset of the former. Two intermediate upland regions show different associations, one with the highlands the other with the lowlands. Lowland faunas, even over large distances, are very similar, and all relate more closely to the western end of the highlands than to the east. Disaggregating the fauna into large and small species shows that the former show a stronger geographical pattern than the latter; most universally distributed species are small. Latitudinal variation in regional richness, and longitudinal differentiation among highland faunas relate to distance from glacial refugia. This is not reflected in site species richness, raising questions about the assembly rules for local faunas. The western bias in the relationships of lowland faunas to those of the highlands, the differences between large and small species and the varying rates of faunal turnover within the area studied suggest that patterns of post-glacial dispersal are complex and incompletely understood.

Intermediary disturbance increases tree diversity in riverine forest of southern Brazil

Floods are frequently associated with disturbance in structuring riverine forests and they lead to environmental heterogeneity over space and time. We evaluated the distribution of tree species, ecological groups, species richness and diversity from the point bar to the slope of a riverside forest in southern Brazil (Lat. 30°01′S, Long. 52°47′W) to analyze the effects of flooding on soil properties and forest structure. A plot of 50 × 200 m divided in five contiguous transects of 10 × 200 m parallel to the river was installed, where we measured all the individual trees with pbh ≥ 15 cm. A detailed topographical and soil survey was carried out across the plot and indicated significant differences in organic matter and most mineral nutrients through the topographical gradient. The 1,229 surveyed individuals belonged to 72 species and 35 families. We used Partial CCA and Species Indicator Analysis to observe the spatial distribution of species. Both analyses showed that species distribution was strongly related to the flooding gradient, soil properties and also by space and pure spatial structuring of species and environmental variables (spatial autocorrelation), although a large part of variation remains unexplained. The ecological groups of forest stratification, plant dispersal and requirements for germination indicated slight differences among frequently, occasional and non-flooded transects. Species richness and diversity were higher at intermediate elevations and were associated to the increased spatial–temporal environmental heterogeneity. Across the plot, the direct influence of flooding on tree species distribution created a vegetation zonation that is determined by predicted ecological traits.

Epilithic diatom assemblages and their relationship to environmental characteristics in an agricultural watershed (Guadiana River, SW Spain)

Abstract Large-scale patterns of benthic diatom assemblages were analyzed in an agricultural basin, the Guadiana River. The distribution patterns of epilithic diatom assemblages were analyzed at different spatial scales: the whole watershed, the upper calcareous subcatchment and the mid-lower siliceous subcatchment. At the whole watershed scale, two major ecological gradients were revealed. The first one summarized the diatom distribution throughout a nutrient concentration gradient, while the second gradient was related to the geological structure of the watershed. Variance partitioning allowed the effects of the different sets of environmental parameters related to every CCA gradient to be separated. Analyzing the subcatchment gradients with partial CCA allowed us to define specific key factors that affect diatom species composition. Although water chemistry consistently played the most important role in structuring diatom assemblages in the Guadiana, spatial factors such as altitude or geographic location also explained some variation in diatom distribution.

South Patagonian ombrotrophic bog vegetation reflects biogeochemical gradients at the landscape level

AbstractQuestion: Which environmental variables affect the floristic composition of south Patagonian bog vegetation along a gradient of climate and biogeochemical changes with increasing distance from the Pacific ocean?Location: Trans‐Andean transect (53° S), southern PatagoniaMaterial and Methods: Floristic composition, peat characteristics (water level, decomposition, pH, total nitrogen, total carbon, ash content and plant available P, K, Na, Ca, Mg, Fe, Mn, Zn, and Al) and climatic constraints of ombrotrophic peat‐lands were measured at 82 plots along a gradient of increasing distance from the Pacific Ocean.Results: Climatic constraints and biogeochemical peat characteristics significantly change with increasing distance from the Pacific. Peatland vegetation shifted from hyperoceanic blanket bogs dominated by cushion forming vascular plants to the west to Sphagnum bogs to the east. Climatic and biogeochemical variables explained a large proportion of the floristic variation along the first DCA axis. The second axis represented a water level gradient. When ‘distance to the Pacific’ was defined as a covariable in partial CCA, the proportion of variance explained declined for most other variables, especially in the case of annual precipitation and exchangeable base cations and related traits. The differences in biogeochemical characteristics related to peat were mainly attributed to the input of sea‐borne cations.Conclusions: While variation in vegetation composition along a longitudinal gradient crossing the southern Andes was attributed to climatic constraints as expected, vegetation composition was also strongly affected by the biogeochemical characteristics of peat. Sea spray was of high ecological importance to peat chemistry and, consequently, to floristic composition. Presumably, south Patagonian peat bogs represent a glimpse of pre‐industrial environments, so that these peat bogs may act as reference systems with respect to atmospheric inputs in mire ecology research.

The role of fungal taxa and developmental stage of mushrooms in determining the composition of the mycophagous insect community in a Japanese forest

We hypothesize that differences in fungal taxonomic groups may exert a direct influence on the composition of myco- phagous insect communities, and that the relative importance of taxonomy compared to other fungal traits may change as the mush- rooms decay. We conducted a 3-year field survey and analyzed the species composition of mycophagous insect communities using partial canonical correspondence analysis (partial CCA). We collected 2457 mushrooms belonging to 27 genera, and 4616 insects belonging to 16 families emerged from 439 of the mushrooms. For the whole insect community, fungal genera explained 10-19% of the total variance in the family composition of the insect communities of mushrooms at different developmental stages. Only the fungal genus Collybia significantly affected the community composition almost irrespective of developmental stage. In the droso- philid community, which consisted of 844 individuals from 9 species, fungal genera explained 19-34% of the total variance. Some fungal genera, such as Amanita and Collybia, affected the drosophilid community, but not at all developmental stages. The number of fungal genera that significantly affected the insect community composition did not differ among fungal stages both in the whole insect community and in the drosophilid community. Thus, our former hypothesis was supported by the present analysis, whereas the latter was not. However, the percentages of variance explained by fungal genera were rather small. This suggests that the importance of fungal genera is likely to be less significant than that of other selection pressures in determining the species composition of myco- phagous insect communities.

Mangrove pollen of Indonesia and its suitability as a sea-level indicator

We investigated the mangroves of Southeast Sulawesi, Indonesia, to assess their potential as proxies for reconstructing sea level during the Holocene. Initial investigations confirmed that the mangrove species demonstrate zonations parallel to the shoreline and are dominated by the family Rhizophoraceae with Avicennia, Heritiera and Sonneratia also important constituents of the mangroves. We investigated the vertical distributions of pollen assemblages at three sites. Partial CCA analysis demonstrated that at all three sites, elevation was a significant control on the distribution of pollen assemblages in surface samples. We combined the three contemporary transects to develop a regional transfer function to explain the relationship between the surface assemblages and elevations using the Maximum Likelihood (ML) method. The developed transfer function indicated mangrove pollen can be used as a precise indicator of past sea levels with an error of ± 0.22 m. The transfer function was applied to two fossil cores from the Wakatobi Marine National Park and evaluated using the Modern Analogue Technique. Both cores showed similar patterns in changes of palaeo mangrove elevation and had modern analogues in the contemporary training set. We conclude that mangrove pollen is a suitable proxy for reconstructing sea level in tropical environments.

Impact of site and management on the diversity of central European mesic grassland

The main objective was to quantify the relative impact of current management types on plant species-richness and composition of mesic grasslands with regard to other important determinants such as topography, soil chemical parameters and grassland age. The grasslands were (i) differentiated into management types and vegetation types, (ii) these types were tested for differences in site conditions and species-richness, and (iii) the relative impact of management, site conditions, grassland age and regional scale geomorphology on floristic composition was quantified. TWINSPAN classification of the vegetation separated nutrient-poor from nutrient-rich sites. Results of ANCOVA revealed that vegetation types indicating high nutrient levels showed significantly higher contents of plant available phosphorous and younger grassland age. In partial CCA analyses, the geomorphology accounted for almost one third of explained variance. The current management had a relatively low explanatory value. Soil chemical variables and topography, in contrast, explained together almost twice as much variation in floristic composition.

Seed bank diversity in mesic grasslands in relation to vegetation type, management and site conditions

Questions: 1. Do different management types (i.e. hay meadow, silage meadow, meadow-pasture, pasture) have different impact on the size and composition of the seed bank of mesic grassland (Arrhenatheretalia)? 2. How strong is the effect of management on the seed bank in relation to above-ground vegetation, edaphic factors and land-use history? 3. Are there differences in C-S-R plant strategy types and seed longevity under different management regimes? Location: Lahn-Dill Highlands in central-western Germany. Methods: Above-ground vegetation and the soil seed bank of 63 plots (at 21 sites) in mesic grasslands were studied. Differences between management types in quantitative seed bank traits and functional characteristics were tested by ANOVA. The impact of management, above-ground vegetation, site conditions and land-use history on seed bank composition were analysed by partial CCA. Results: Management had no significant impact on species richness and density of the seed bank but significantly influenced their floristic composition and functional characteristics. CCA revealed that even after adjustment for soil chemical parameters and above-ground vegetation management still had significant impact on seed bank composition. ANOVA revealed that silage meadows contained higher proportions of R-strategy compared to hay meadows. In contrast, in hay meadows and meadow-pastures proportions of S-strategy were higher than in silage meadows. Conclusions: The type of grassland management has little impact on quantitative seed bank traits. Management types with a high degree of disturbance lead to an increase of species following a ruderal strategy in the seed bank. Irrespective of management type only a limited proportion of characteristic grassland species is likely to re-establish from the seed bank after disappearance from above-ground vegetation.