Balance Of Parts Research Articles

Ventral tegmental area amylin / calcitonin receptor signaling suppresses feeding and weight gain in female rats

The pancreatic peptide amylin promotes negative energy balance in part through activation of amylin receptors (AmyRs) expressed in the ventral tegmental area (VTA), but studies have been limited to male rodents. We evaluated whether VTA amylin signaling governs feeding and body weight in female rats. Indeed, pharmacological VTA AmyR activation suppressed chow intake and body weight in females. Viral-mediated knockdown of VTA calcitonin receptor (GPCR of AmyR) supports the physiological relevance of VTA amylin signaling for energy balance control in females. Collectively, these data support the relevance of VTA amylin signaling for energy balance control in both sexes.

Laboratory experiments can pre-design to address power and selection issues

In this paper, motivated by aspects of preregistration plans we discuss issues that we believe have important implications for how experiments are designed. To make possible valid inferences about the effects of a treatment in question, we first illustrate how economic theories can help allocate subjects across treatments in a manner that boosts statistical power. Using data from two laboratory experiments where subject behavior deviated sharply from theory, we show that the ex-post subject allocation to maximize statistical power is closer to these ex-ante calculations relative to traditional designs that balances the number of subjects across treatments. Finally, we call for increased attention to (i) the appropriate levels of the type I and type II errors for power calculations, and (ii) how experimenters consider balance in part by properly handling over-subscription to sessions.

Analysis of the long-term effects of groundwater extraction on the water balance in part of the Urucuia Aquifer System in Bahia - Brazil

In agricultural regions where there is insufficient rainwater for cultivation, understanding the dynamics of surface water and groundwater is critical to assess the impact of increased well pumping on the water balance. The western region of the state of Bahia-Brazil, the largest area of agribusiness in the state, has experienced progressive occupation since the 1980s, resulting in pressure on water resources - mainly after the introduction of irrigation - and conflicts among water users. This study analyzed the effects of groundwater extraction by wells in a portion of the Urucuia Aquifer System. The methodology used was the simulation of groundwater flow in steady-state for three scenarios: i) without withdrawal of water by pumping wells; (ii) with current withdrawal rates; and (iii) with 60% additional extraction. After defining well production rates from field surveys (4.6 m3 s-1) and modeling of the aquifer base geometry (maximum thickness of 535 m), the steady-state models with and without pumping show that, in the long term, groundwater storage decreases by 2 km3 (0.8 m on average in the aquifer) without interference in the regional flow direction. The mass balance shows that the base flow of the main rivers is reduced by approximately 6% after current groundwater extraction and an additional 2.5% after additional extraction. The results point to a greater impact on surface water caused by a reduction in groundwater storage and related river base flow. This indicates the importance of maintaining and expanding the groundwater-level monitoring network.

Social Justice for the Advantaged: Freedom from Racial Equality Post-Milliken

Background/ContextIn Milliken v. Bradley (1974), the U.S. Supreme Court deemed unconstitutional a metropolitan-wide desegregation plan in Detroit that sought to achieve racial balance in part by busing white suburban students to the city's majority black schools. In a stark departure from Brown v. Board of Education of Topeka (1954), Milliken left the question of how, or even whether, to equalize education for black students up to local parents, educators, activists, school board members, state legislators, and other private and public community stakeholders.Purpose/Objective/Focus of StudyIn this article, I consider school desegregation as a form of social justice for blacks and racial equality for all, 40 years post-Milliken. Drawing from research on school desegregation as social justice and Bell's theory of interest convergence, I argue that integration and equality in the post-Civil Rights Era requires attention to the competing visions of social justice I describe as black equality and white freedom.Research DesignFramed by distributional and relational dimensions of social justice and Bell's theory of interest convergence, this paper presents a conceptual analysis of school desegregation as social justice post-Milliken.Conclusions/RecommendationsThe competing interests of black equality and white freedom central to Milliken remain fundamental to the question of school desegregation 40 years later. In light of growing political and public support for local control, school choice, and neighborhood schools, I conclude by discussing the implications of local control for black students and racial equality within the current context of widening economic equality, political polarization, and racial isolation in the United States and the world.

Abstract P168: ChIP-seq Analysis of Genomic Binding Sites for the Transcription Factor Fosl2 in Kidney Collecting Duct Cells

Kidney collecting duct principal cells play a key role in blood pressure regulation and water balance in part through the action of vasopressin. We integrated data from prior proteomics and transcriptomics studies of cultured mouse mpkCCD cells using Bayes’ Rule to rank transcription factors (TFs) likely to be involved in vasopressin-mediated transcriptional regulation. The top ranked TF was the AP-1 component Fos-like 2 (Fosl2) and the third ranked TF was Sox4. We carried out ChIP-seq analysis (HiSeq 2000 sequencer) to identify genomic sites of Fosl2 binding using an antibody successfully employed for ChIP-seq in the Mouse ENCODE Project. mpkCCD cells treated with the vasopressin analog dDAVP or vehicle were separately analyzed. The success of the immunoprecipitation of Fosl2 was confirmed by western blotting and LC-MS/MS of the immuno-precipitated material (ChIP-MS). ChIP-MS identified two different Fosl2 peptides, but there were no peptides corresponding to other AP-1 proteins. ChIP-MS also identified Bub1b, a nuclear protein kinase known to phosphorylate histones, as a Fosl2-interacting protein. Fosl2-binding sites successfully mapped to genes by ChIP-seq included a Fosl2 binding site, centered at 87 bp upstream from the transcription start site [TSS] of Sox4, that was strongly downregulated in response to dDAVP, n=2; confirmed by ChIP-PCR. TF binding site prediction (Genomatix) identifies putative Sox4 binding sites in vasopressin-regulated genes beta-ENaC (-822 bp from TSS) and aquaporin-2 (-515 bp from TSS). These data provide an initial step in identification of the vasopressin-regulated transcriptional network in renal collecting duct cells.

Mechanisms by which the orexigen NPY regulates anorexigenic α-MSH and TRH.

Protein posttranslational processing is a cellular mechanism fundamental to the generation of bioactive peptides, including the anorectic α-melanocyte-stimulating hormone (α-MSH) and thyrotropin-releasing hormone (TRH) peptides produced in the hypothalamic arcuate (ARC) and paraventricular (PVN) nuclei, respectively. Neuropeptide Y (NPY) promotes positive energy balance in part by suppressing α-MSH and TRH. The mechanism by which NPY regulates α-MSH output, however, is not well understood. Our results reveal that NPY inhibited the posttranslational processing of α-MSH's inactive precursor proopiomelanocortin (POMC) by decreasing the prohormone convertase-2 (PC2). We also found that early growth response protein-1 (Egr-1) and NPY-Y1 receptors mediated the NPY-induced decrease in PC2. NPY given intra-PVN also decreased PC2 in PVN samples, suggesting a reduction in PC2-mediated pro-TRH processing. In addition, NPY attenuated the α-MSH-induced increase in TRH production by two mechanisms. First, NPY decreased α-MSH-induced CREB phosphorylation, which normally enhances TRH transcription. Second, NPY decreased the amount of α-MSH in the PVN. Collectively, these results underscore the significance of the interaction between NPY and α-MSH in the central regulation of energy balance and indicate that posttranslational processing is a mechanism that plays a specific role in this interaction.

Nociceptin/orphanin FQ suppresses the excitability of neurons in the ventromedial nucleus of the hypothalamus

Nociceptin or orphanin FQ (N/OFQ) stimulates food intake when injected into the ventromedial nucleus of the hypothalamus (VMN). The VMN negatively regulates energy balance in part by tonically activating proopiomelanocortin arcuate neurons, thereby suppressing food intake. However, it is not clear how orexigenic neurotransmission within the VMN can stimulate food intake. We tested the hypothesis that the orexigenic action of N/OFQ results from its inhibition of anorexigenic VMN neurons. We studied the effects of N/OFQ on the electrical properties of anorexigenic VMN neurons in acute brain slices. Ionic mechanisms underlying the actions of N/OFQ were studied using whole cell patch-clamp recordings from VMN neurons expressing the anorexigenic leptin receptor (LepRb). Bath application of N/OFQ to LepRb-expressing VMN neurons elicited a robust, reversible membrane hyperpolarization that suppressed neuronal excitability by raising the action potential firing threshold and cell rheobase. N/OFQ activated a postsynaptic, G-protein coupled, inwardly rectifying potassium (GIRK) current that was sensitive to G-protein inactivation, blocked by the GIRK blocker SCH23390, and occluded by the GABAB agonist and potent GIRK activator, baclofen. Application of the selective N/OFQ receptor antagonist SB-612111 blocked the inhibitory effects of N/OFQ. We concluded that N/OFQ directly inhibited VMN neurons by activating a GIRK. These results implicate the site-specific contributions of orexigenic neuropeptides at VMN neurons to suppress anorexigenic output. This study thus advances our understanding regarding the contributions of the VMN to hypothalamic regulation of energy balance.

A dynamic over-sampling procedure based on sensitivity for multi-class problems

Classification with imbalanced datasets supposes a new challenge for researches in the framework of machine learning. This problem appears when the number of patterns that represents one of the classes of the dataset (usually the concept of interest) is much lower than in the remaining classes. Thus, the learning model must be adapted to this situation, which is very common in real applications. In this paper, a dynamic over-sampling procedure is proposed for improving the classification of imbalanced datasets with more than two classes. This procedure is incorporated into a memetic algorithm (MA) that optimizes radial basis functions neural networks (RBFNNs). To handle class imbalance, the training data are resampled in two stages. In the first stage, an over-sampling procedure is applied to the minority class to balance in part the size of the classes. Then, the MA is run and the data are over-sampled in different generations of the evolution, generating new patterns of the minimum sensitivity class (the class with the worst accuracy for the best RBFNN of the population). The methodology proposed is tested using 13 imbalanced benchmark classification datasets from well-known machine learning problems and one complex problem of microbial growth. It is compared to other neural network methods specifically designed for handling imbalanced data. These methods include different over-sampling procedures in the preprocessing stage, a threshold-moving method where the output threshold is moved toward inexpensive classes and ensembles approaches combining the models obtained with these techniques. The results show that our proposal is able to improve the sensitivity in the generalization set and obtains both a high accuracy level and a good classification level for each class.

CRTC3 links catecholamine signalling to energy balance

The adipose-derived hormone leptin maintains energy balance in part through central nervous system-mediated increases in sympathetic outflow that enhance fat burning. Triggering of β-adrenergic receptors in adipocytes stimulates energy expenditure by cyclic AMP (cAMP)-dependent increases in lipolysis and fatty-acid oxidation. Although the mechanism is unclear, catecholamine signalling is thought to be disrupted in obesity, leading to the development of insulin resistance. Here we show that the cAMP response element binding (CREB) coactivator Crtc3 promotes obesity by attenuating β-adrenergic receptor signalling in adipose tissue. Crtc3 was activated in response to catecholamine signals, when it reduced adenyl cyclase activity by upregulating the expression of Rgs2, a GTPase-activating protein that also inhibits adenyl cyclase activity. As a common human CRTC3 variant with increased transcriptional activity is associated with adiposity in two distinct Mexican-American cohorts, these results suggest that adipocyte CRTC3 may play a role in the development of obesity in humans.

Development of a multi-classification neural network model to determine the microbial growth/no growth interface

Boundary models have been recognized as useful tools to predict the ability of microorganisms to grow at limiting conditions. However, at these conditions, microbial behaviour can vary, being difficult to distinguish between growth or no growth. In this paper, the data from the study of Valero et al. [Valero, A., Pérez-Rodríguez, F., Carrasco, E., Fuentes-Alventosa, J.M., García-Gimeno, R.M., Zurera, G., 2009. Modelling the growth boundaries of Staphylococcus aureus: Effect of temperature, pH and water activity. International Journal of Food Microbiology 133 (1–2), 186–194] belonging to growth/no growth conditions of Staphylococcus aureus against temperature, pH and a w were divided into three categorical classes: growth (G), growth transition (GT) and no growth (NG). Subsequently, they were modelled by using a Radial Basis Function Neural Network (RBFNN) in order to create a multi-classification model that was able to predict the probability of belonging at one of the three mentioned classes. The model was developed through an over sampling procedure using a memetic algorithm (MA) in order to balance in part the size of the classes and to improve the accuracy of the classifier. The multi-classification model, named Smote Memetic Radial Basis Function (SMRBF) provided a quite good adjustment to data observed, being able to correctly classify the 86.30% of training data and the 82.26% of generalization data for the three observed classes in the best model. Besides, the high number of replicates per condition tested ( n = 30) produced a smooth transition between growth and no growth. At the most stringent conditions, the probability of belonging to class GT was higher, thus justifying the inclusion of the class in the new model. The SMRBF model presented in this study can be used to better define microbial growth/no growth interface and the variability associated to these conditions so as to apply this knowledge to a food safety in a decision-making process.

Rapid Rewiring of Arcuate Nucleus Feeding Circuits by Leptin

The fat-derived hormone leptin regulates energy balance in part by modulating the activity of neuropeptide Y and proopiomelanocortin neurons in the hypothalamic arcuate nucleus. To study the intrinsic activity of these neurons and their responses to leptin, we generated mice that express distinct green fluorescent proteins in these two neuronal types. Leptin-deficient (ob/ob) mice differed from wild-type mice in the numbers of excitatory and inhibitory synapses and postsynaptic currents onto neuropeptide Y and proopiomelanocortin neurons. When leptin was delivered systemically to ob/ob mice, the synaptic density rapidly normalized, an effect detectable within 6 hours, several hours before leptin's effect on food intake. These data suggest that leptin-mediated plasticity in the ob/ob hypothalamus may underlie some of the hormone's behavioral effects.

Music and Storytelling in the Tuvan Shamanic World

This article discusses the interaction of music and storytelling with shamanism in Tuva, when practiced by shamans, and also by other people. The arts form an important layer of the shamanic context, which is today out of balance in part because outsiders focus so strongly on shamans and throat-singing, ignoring other cultural areas. Important aspects of storytelling are imagery, spiritual geography, the soul journey, and the persona of the teller. Music also contributes imagery and sound healing. It creates a bridge to the spiritual world and calls spirits through timbres produced with instruments and the voice.

Arginine vasopressin stimulates atrial natriuretic peptide gene expression and secretion from rat diencephalic neurons

The central nervous system modulates cardiovascular function and fluid and electrolyte balance in part through the actions of vasoactive peptides/neurotransmitters. The presence of several vasoactive peptides and their receptors in the hypothalamus suggests a possible interaction at this site. One level at which vasoactive peptides such as arginine vasopressin (AVP) and atrial natriuretic peptide (ANP) might interact is through the mutual regulation of production and secretion in the hypothalamus. To determine whether AVP modulates ANP gene expression and secretion, we cultured fetal rat diencephalic neurons in the presence of AVP. AVP induced a significant increase in ANP secretion in dose-related fashion (mean +/- SEM basal ANP, 87 +/- 4 pg/ml; maximal mean AVP-stimulated ANP, 146 +/- 6 pg/ml; P less than 0.05, by analysis of variance). Neither oxytocin nor the vasoactive neuropeptide angiotensin-II had any effect on ANP secretion. The stimulatory effect of AVP was significantly blocked by coincubation with a V1 receptor antagonist, but was unaffected by a V2 receptor antagonist. The immunoreactive ANP secreted in response to AVP was the major brain isoform, ANP-(103-126). Coincubation with a calcium channel antagonist, nifedipine, had no effect on AVP-induced ANP secretion, while ryanodine, an inhibitor of intracellular calcium mobilization, significantly reduced the stimulatory effect of AVP. AVP induced a dose-related, nearly 3-fold maximal increase in ANP mRNA expression at 4 h. Coincubation of the neurons with a V1 receptor antagonist also significantly attenuated the increased ANP gene expression induced by AVP. These results indicate that AVP acts directly through V1 receptors on cultured fetal rat diencephalic neurons to augment ANP gene expression and secretion of the peptide. The effects are probably related to AVP-stimulated mobilization of intracellular calcium and not the result of calcium influx into the cell. These studies provide the first evidence that AVP modulates ANP production from cultured neurons. In the central nervous system, these two vasoactive neuropeptides might interact in part through the regulation of ANP production by AVP.

Arginine vasopressin stimulates atrial natriuretic peptide gene expression and secretion from rat diencephalic neurons.

The central nervous system modulates cardiovascular function and fluid and electrolyte balance in part through the actions of vasoactive peptides/neurotransmitters. The presence of several vasoactive peptides and their receptors in the hypothalamus suggests a possible interaction at this site. One level at which vasoactive peptides such as arginine vasopressin (AVP) and atrial natriuretic peptide (ANP) might interact is through the mutual regulation of production and secretion in the hypothalamus. To determine whether AVP modulates ANP gene expression and secretion, we cultured fetal rat diencephalic neurons in the presence of AVP. AVP induced a significant increase in ANP secretion in dose-related fashion (mean +/- SEM basal ANP, 87 +/- 4 pg/ml; maximal mean AVP-stimulated ANP, 146 +/- 6 pg/ml; P less than 0.05, by analysis of variance). Neither oxytocin nor the vasoactive neuropeptide angiotensin-II had any effect on ANP secretion. The stimulatory effect of AVP was significantly blocked by coincubation with a V1 receptor antagonist, but was unaffected by a V2 receptor antagonist. The immunoreactive ANP secreted in response to AVP was the major brain isoform, ANP-(103-126). Coincubation with a calcium channel antagonist, nifedipine, had no effect on AVP-induced ANP secretion, while ryanodine, an inhibitor of intracellular calcium mobilization, significantly reduced the stimulatory effect of AVP. AVP induced a dose-related, nearly 3-fold maximal increase in ANP mRNA expression at 4 h. Coincubation of the neurons with a V1 receptor antagonist also significantly attenuated the increased ANP gene expression induced by AVP. These results indicate that AVP acts directly through V1 receptors on cultured fetal rat diencephalic neurons to augment ANP gene expression and secretion of the peptide. The effects are probably related to AVP-stimulated mobilization of intracellular calcium and not the result of calcium influx into the cell. These studies provide the first evidence that AVP modulates ANP production from cultured neurons. In the central nervous system, these two vasoactive neuropeptides might interact in part through the regulation of ANP production by AVP.

Growth hormone and renal glutamine and glutamate handling.

Growth hormone administration effects a positive nitrogen balance in part by recycling glutamine nitrogen as glutamate at the expense of ureagenesis. The study presented here focuses on the response of the isolated perfused hypophysectomized rat kidney to acute growth hormone administration during infusions of either glutamine or glutamate. Growth hormone at 50 nM acutely decreases the renal utilization of both glutamine and glutamate while enhancing reabsorption of the latter. During glutamine infusions of either 1,000 or 500 nmol/min, growth hormone markedly reduced net glutamine utilization by 55% at the high loads and reversed utilization to release at the lower load; associated with decreased glutamine utilization was reduced ammonium production and increased glutamate release. Although glutamine reabsorption was unchanged, glutamate reabsorption increased and NH4+ excretion decreased. During glutamate infusion of 180 nmol/min, growth hormone reduced glutamate utilization 66%, the residual utilization matching increased glutamate reabsorption was associated with enhanced bicarbonate reabsorption and a redistribution of NH4+ release into the urine; all three responses were eliminated by amiloride. These responses to growth hormone are consonant with reduced glutamate oxidation underlying decreased glutamine utilization and accelerated luminal Na+-H+ exchange mediating luminal transport, events that are conceivably interrelated.

Comparative Studies in Selected Species of Eucalyptus Used in Rehabilitation of the Northern Jarrah Forest, Western Australia. I. Patterns of Xylem Pressure Potential and Diffusive Resistance of Leaves

Land use which reduces tree canopy density and the impact of Phytophthora cinnamomi are believed to be altering the hydrological balance of parts of the northern jarrah forest, Western Australia. In the drier eastern zones of the forest, replacement plant communities must maintain the soil-salt-water balance to prevent significant increases in salinization of streams in water supply catchments. Daily and seasonal patterns of the diffusive resistance of leaves and xylem pressure potential were determined for the major natural dominant of the region, Eucalyptus marginata, and five other species of Eucalyptus used in rehabilitation. Three types of daily and seasonal patterns were observed. E. marginata and E. calophylla exhibited little stomatal control of water loss, and leaf resistances remained low throughout the study period (type 1). E. maculata, E. resinifera and E. saligna exhibited marked stomatal regulation during summer days when xylem pressure potentials fell below -2.O MPa (type 2). E. wandoo (type 3) also controlled water loss but developed xylem pressure potentials far lower than all other species tested (<-3.0 MPa). Although none of the species tested replicated the summer stomatal resistance and xylem pressure potential patterns of E. marginata, it is suggested that total annual water use should be examined before selecting the most appropriate species to rehabilitate disturbed sites in the eastern zones of the northern jarrah forest region.

Tertiary extension north of the Las Vegas Valley shear zone, Sheep and Desert Ranges, Clark County, Nevada

Detailed mapping reveals the presence of high-angle extensional faults and low-angle gravity slides on the west side of the Sheep Range. Three major high-angle faulting events each account for 20° of eastward rotation and accommodate extension between the Las Vegas Range and the Desert Range. Low-angle faults represent surficial slides in response to topography produced by extension on the high-angle faults. Faulting took place during the Miocene, synchronously with deposition of the Horse Spring Formation and with displacement on the Las Vegas Valley shear zone. The extension in the Sheep Range took place without volcanism, intrusion, or metamorphism of the Paleozoic sedimentary rocks. Offset thrust faults suggest that the area west of the Sheep Range extended almost 100% during the Miocene, while the corresponding area south of the Las Vegas Valley shear zone did not extend significantly. The shear zone bounded the extending terrane on the south, acting as a transform fault. This extension west of the Sheep Range may in part balance that mapped by Anderson (1971) in the Eldorado Mountains. The Las Vegas Valley shear zone and the Lake Mead fault system may have acted together to compensate for areas of localized extension between the Colorado Plateau and the vicinity of the Specter Range.

Perception of the Structural Balance of Part of the International System of Nations

Perception of the Structural Balance of Part of the International System of Nations

Geological Sketches at Home and Abroad Text-Book of Geology

II. We now come to consider the quality of the matter contained in the volume, the discrimination exercised in its selection, the validity of the theories presented, and the fidelity with which the science is portrayed. It is the function of a text-book to exhibit to the student an impartial and symmetric outline of the science. Its author is under obligation to present the views which are generally entertained by the great body of geologists, carefully withholding those which are peculiar to himself. From the great mass of available matter he must select that which will afford a well-balanced and comprehensive review, and he must sedulously avoid giving undue prominence to those matters which have special interest to himself by reason of his individual studies. In the work before us this has been accomplished in a manner which may truly excite admiration. Although the author is an original investigator in several departments of the science he delineates, he has permitted his own predilections to give little if any additional prominence to his special topics, and the wisdom he has displayed in the selection of material and the balancing of parts will commend itself to all professional readers. Geological Sketches at Home and Abroad. By Archibald Geikie, LL.D., F.R.S. With Illustrations. (London and New York: Macmillan and Co., 1882.) Text-Book of Geology. By Archibald Geikie, LL.D., F.R.S. With Illustrations. (London: Macmillan and Co., 1882.)