Respective Parent Research Articles

Exploring Problematic Smartphone Use (PSU) among Muslim Adolescents in Southern Thailand: Students and Parents’ Insights

This study aimed to explore problematic smartphone use (PSU) among young Muslim students in Southern Thailand. This research employed a dual-perspective, phenomenological approach, examining PSU from the perspectives of both adolescent Muslim students and their parents. We interviewed 10 participants, through purposive sampling, comprising 5 adolescent Muslim students studying in Southern Thailand and their respective parents. The qualitative data collected was then subjected to thematic analysis. Four themes emerged from the thematic analysis. Each theme was further explored and discussed based on its categories and subcategories. The first theme, “Primary Purposes of Using Smartphones”, consisted of: learning, communicating, and relaxation. The second theme, “Types of PSU”, included overusing smartphones and dysfunctional habits. The third theme, “Negative Impacts of PSU”, encompassed physical effects and losing control. The last theme, “Reasons for PSU”, was composed of psychological needs and low regulation as its categories. Moreover, the study highlighted similarities and differences in perspectives on these themes between Muslim adolescents and their parents. The findings and limitations of this research are presented to suggest potential directions for future study and implications.

Translation, Cultural Adaptation, and Validation of the Arab Version of the Adolescent Food Parenting Questionnaire (AFPQ) in Kut City, Iraq.

Background Food parenting behaviors have been increasingly critical to adolescent nutritional health. These behaviors play a decisive role in shaping food intake and weight status in adolescents through autonomy support, coercive control, modeling habits, healthy structure, and determining snacking patterns. To our knowledge, there is no available food parenting questionnaire in the Arabic version; therefore, a reliable and validated Arabic version of the questionnaire to assess food parenting behavior among Iraqi adolescents is required. The aim of this study was to translate, culturally adapt, and validate the Adolescent Food Parenting Questionnaire (AFPQ) into Arabic (AFPQ-A) for adolescents aged 16 to 18 in Kut City, Iraq. Methodology The original version of AFPQ was translated according to a 10-step protocol after being authorized by the developer. The modified kappa statistic (κm), the sum content validity index/universal agreement (S-CVI/UA) method, the scale-level content validity index average (S-CVI/Ave), and the item-level content validity index (I-CVI) were used by four independent experts to assess the content validity of the AFPQ for adolescents and parenting. Face validity was assessed among the adolescents (n = 30) and their respective parents. Results The translation process into the Arabic version produced minor modifications to the original questionnaire. The S-CVI/Ave score for the parent version was 0.95, while the I-CVI/UA score was 0.81 for the adolescent version. The κm values of 1.00 were found in the majority of the items. These scores indicated that the AFPQ items possessed good content validity. Both the parent and adolescent versions demonstrated excellent face validity, with the sum of face validity index/average (S-FVI) scores of 0.95 and 0.94, respectively. Conclusion The AFPQ-A has been demonstrated to be a valid and reliable instrument for the assessment of food parenting behaviors in Iraqi adolescents.

Effects of increased availability and economic incentives on preadolescents’ healthier beverages choices: An experimental study

This field experimental study examined how increased availability and economic incentives for healthier beverages affect preadolescents’ actual healthier beverage choices in a real-world setting. In addition, the impact of preadolescents’ descriptive norm towards healthier beverages, price awareness and parental restrictive rules regarding beverage consumption were explored. During the experiment, preadolescents could independently buy two beverages from an assortment with a total of eight beverages. A 2x2 between-subjects design was used, manipulating the presence of increased availability of healthier beverages and economic incentives (taxes for unhealthier beverages and subsidies for healthier beverages). A total of 305 preadolescents between 8 and 14 years old (M = 10.18, SD = 1.74) participated, each accompanied by one of their respective parents. Results showed that preadolescents were more likely to choose healthier beverages and perceived a higher descriptive norm towards healthier beverages when the assortment included increased relative availability of healthier beverages. Economic incentives and price awareness did not impact healthier beverage choices. Preadolescents were less likely to choose healthier beverages when parents reported higher levels of restrictive rules regarding beverage consumption. Results of the study suggest that increasing relative availability of healthier options could be a promising intervention strategy to support preadolescents in making healthier choices.

Spin states of X-complex asteroids in the inner main belt

Context. Based on the V-shape search method, two families, Athor and Zita, have been identified within the X-complex population of asteroids located in the inner main belt. The Athor family is ~3 Gyr old while the Zita family could be as old as the Solar System. Both families were found to be capable of delivering near-Earth asteroids (NEAs). Moreover, the Athor family was linked to the low-iron enstatite (EL) meteorites. Aims. The aim of our study is to characterise the spin states of the members of the Athor and Zita collisional families and test whether these members have a spin distribution consistent with a common origin from the break up of their respective family parent asteroids. Methods. To perform this test, our method is based on the well-established asteroid family evolution, which indicates that there should be a statistical predominance of retrograde-rotating asteroids on the inward side of family’s V-shape, and prograde-rotating asteroids on the outward side of family’s V-shape. To implement the method, we used photometric data from our campaign and the literature in order to reveal the spin states, and hence their rotation sense (prograde or retrograde), of the asteroids belonging to these families. We combined dense and sparse-in-time photometric data in order to construct asteroid rotational light curves; we performed the light curve inversion method to estimate the sidereal period and 3D convex shape along with the spin axis orientation in space of several family member asteroids. Results. We obtained 34 new asteroid models for Athor family members and 17 for Zita family members. Along with the literature and revised models, the Athor family contains 60% (72% considering only the family’s core) of retrograde asteroids on the inward side and, conversely, 76% (77% considering only the family’s core) of prograde asteroids on the outward side. We also found that the Zita family exhibits 80% of retrograde asteroids on the inward side. In addition, the Zita family presents an equal amount of prograde and retrograde rotators (50% each) on the outward side. However, when we applied Kernel density estimation (KDE), we also found a clear peak for prograde asteroids on the outward side, as expected from the theory. Conclusions. The spin states of these asteroids validate the existence of both families, with the Athor family exhibiting a stronger signature for the presence of retrograde-rotating and prograde-rotating asteroids on the inner and outer side of the family, respectively. Our work provides an independent confirmation and characterisation of these very old families, whose presence and characteristics offer constraints for theories and models of the Solar System’s evolution.

Proxy- and self-report evaluation of quality of life in cerebral palsy: Using Spanish version of CPQOL for Children and adolescents

BackgroundPromoting quality of life (QoL) is one of the main goals in interventions carried out with children and adolescents with cerebral palsy (CP). AimsThe aim of this study was to analyze the determinants of QoL in children with CP, including evaluations by the children themselves and their parents, and to identify discrepancies between evaluators. Methods and proceduresThe adapted Spanish version of the Cerebral Palsy Quality of Life (CP-QOL) for children and adolescents (self-report and primary caregiver-reports versions) was applied to a sample of 74 children with CP and their respective parents (totaling 222 participants), as well as instruments to measure functioning (i.e., GMFCS, MACS, CFCS and EDACS). The average age of the children was 12.50 (SD=4.07), with a higher number of boys (55.7 %). Outcomes and resultsThe lowest QoL levels were found in the Functional dimension in both assessments (Mchildren/adolescents=70.21, Mparents=58.14). For children, the highest rated dimension was Social Well-being (M=74.54), while for parents it was School (M=71.03). The degree of agreement between evaluators was low in almost all dimensions (ICC≤.40). More satisfactory predictive models were constructed from the evaluations carried out by parents, except in the case of the Access to Services dimension, with functioning measures being the main predictors of QoL levels. Conclusions and implicationsThe CP-QOL in its two available versions is a useful and specific instrument for assessing QoL in children with CP in both research and professional fields.

Interrogating the Role of Endocytosis Pathway and Organelle Trafficking for Doxorubicin-Based Combination Ionic Nanomedicines.

We have studied the endocytic mechanisms that determine subcellular localization for three carrier-free chemotherapeutic-photothermal (chemo-PTT) combination ionic nanomedicines (INMs) composed of doxorubicin (DOX) and an near-infrared (NIR) dye (ICG, IR820, or IR783). This study aims to understand the cellular basis for previously published enhanced toxicity results of these combination nanomedicines toward MCF-7 breast cancer cells. The active transport mechanism of INMs, unlike free DOX, which is known to employ passive transport, was validated by conducting temperature-dependent cellular uptake of the drug in MCF-7 cells using confocal microscopy. The internalization pathway of these INMs was further probed in the presence and absence of different endocytosis inhibitors. Detailed examination of the mode of entry of the carrier-free INMs in MCF-7 cells revealed that they are primarily internalized through clathrin-mediated endocytosis. In addition, time-dependent subcellular localization studies were also investigated. Examination of time-dependent confocal images indicated that the INMs targeted multiple organelles, in contrast to free DOX that primarily targets the nucleus. Collectively, the high cellular endocytic uptake in cancerous cells (EPR effect) and the multimode targeting ability demonstrated the main reason for the low half-maxima inhibitory concentration (IC50) value (the high cytotoxicity) of these carrier-free INMs as compared to their respective parent chemo and PTT drugs.

Implication of Religious Character Program in Improving Self-Control at State Vocational High School 1 Pedan

<em>The ability of strong self-control can fortify faith for young people in the current era, starting from the concerns of Islamic religious education teachers at State Vocational High School 1 Pedan, sparking the implications of religious character programs aimed at increasing the faith of students to be strong, broad-minded, and have full awareness of understanding right and wrong actions. This study aims to describe the various activities carried out in the implications of religious character programs as a way to improve the religious character of students at State Vocational High School 1 Pedan. This research uses qualitative methods (field research). This research was conducted with data collection techniques through interviews, observations, and documents. Activities are carried out both daily, monthly and annually. Daily activities begin with praying both before and after learning, reading the Qur'an and short surahs, literacy, duha prayers, zuhur prayers, and school spiritists at school, if at home filled with TPA activities, tadarus, and daily amaliyah checks, which are monitored directly by their respective parents. The weekly activity called qolbu management begins with reading the Qur'an together and is filled with religious wisdom stories by the father and mother of Islamic religious education teachers and invited teachers from outside which are carried out on Friday of the first week of each month. Annual activities such as flash pesantren, Isra' Mi'raj commemoration, and the celebration of the Islamic holiday of Eid al-Fitr celebration which is filled with a gathering event between school residents.</em>

Collaborative practice with parents in occupational therapy for children: A scoping review.

In childhood intervention, parent-therapist collaboration is central to the family-centred approach. Despite long-standing discussion in occupational therapy literature, the field faces challenges, including inconsistent terminology and difficulties in translating theory into practice. This paper represents the first part of a comprehensive scoping review study aimed at developing foundational concepts for collaborative practices with parents in occupational therapy for children. Therefore, this paper focusses on mapping existing practices, types, and approaches articulated in the literature. We searched English-language sources published worldwide from 1998 to 2022 discussing collaborative practices with parents in occupational therapy for children aged 0-10 with any diagnosis, including multidisciplinary practices. Seven databases were searched. Data from peer-reviewed indexed literature, theses and dissertations, and book chapters were extracted and analysed through basic numerical and descriptive analyses before being synthesised into similar categories. The Joanna Briggs Institute Manual and the Preferred Reporting Items for Systematic reviews and Meta-Analysis-extension for Scoping Reviews were used. The scoping review yielded 299 papers. Parent-therapist collaboration was prominent in clinics, family homes, schools, and hospitals, particularly during therapy implementation, goal setting, and planning. Most reported practice types included 'interventions with parent engagement', 'parent-directed interventions', and 'parent education'. 'Family-centred', 'occupational-focussed', and 'client-therapist collaborative' approaches were frequently mentioned. There were inconsistencies in the terms used to describe collaborative practice characteristics. Over the past 24 years, the collaborative practice literature has expanded and evolved, with parent-therapist collaboration observed across various occupational therapy settings. Inconsistencies in this collaboration across different therapeutic stages were revealed, which could impact intervention success and sustainability. Further research is needed to explore parent-therapist collaboration mechanisms within and across stages. This scoping review also underscores the need for a common framework to guide practice and research. This literature review explores how occupational therapists and parents work together in childhood intervention. Collaboration is essential for understanding and meeting children's needs within their family and community settings. However, occupational therapists and parents face challenges in applying family-centred practices and using a common language to bridge theory with practice. To address these challenges, we examined 299 papers published between 1998 and 2022 to understand how collaborative practices with parents have been described in the literature. Our review revealed that therapists and parents collaborate across various settings, such as clinics, schools, homes, and hospitals, mainly during therapy sessions, goal setting, and planning interventions. Collaborative practices take different forms. For instance, therapists often encourage parents to actively engage and take the lead in therapy, requiring therapists to recognise and respect parents' priorities and learning preferences. They often develop strategies together to support the child within family routines. While we found several studies on therapist-parent collaboration, the review outlined inconsistencies in how this practice was described and applied, which could affect its success. Therefore, more research is needed to understand the best ways in which collaboration can occur at each stage of therapy. The need for a core guideline for collaborative practice with parents in occupational therapy was also observed. While therapist-parent collaboration is used in occupational therapy for children, there is a clear need to minimise inconsistencies and gaps found in the literature, as well as to ensure a common language to promote intervention quality and success.

Synthesis of biologically active cefpodoxime and vanillin-based schiff base metal complexes with the detailed biological evaluations.

Schiff bases of existing antimicrobial drugs are an area, which is still to be comprehensively explored to improve drug efficiency against consistently resisting bacterial species. In this study, we have targeted a new and eco-friendly method of condensation reaction that allows the "green synthesis" as well as improved biological efficacy. The transition metal complexes of cefpodoxime with well-enhanced biological activities were synthesized. The condensation reaction product of cefpodoxime and vanillin was further reacted with suitable metal salts of [Mn (II), Cu (II), Fe (II), Zn (II), and Ni (II)] with 1:2 molar ratio (metal: ligand). The characterization of all the products were carried out by using UV-Visible, elemental analyzer, FTIR, 1H-NMR, ICP-OES, and LC-MS. Electronic data obtained by UV-Visible proved the octahedral geometry of metal complexes. The biological activities Schiff base ligand and its transition metal complexes were tested by using in-vitro anti-bacterial analysis against various Gram-negative, as well as Gram-positive bacterial strains. Proteinase and protein denaturation inhibition assays were utilized to evaluate the products in-vitro anti-inflammatory activities. The in vitro antioxidant activity of the ligand and its complexes was evaluated by utilizing the 2,2-diphenyl-1-picrylhydrazyl(DPPH) in-vitro method. The final results proved metal complexes to be more effective against bacterial microorganisms as compared to respective parent drug as well as their free ligands. Patch Dock, a molecular docking tool, was used to dock complexes 1a-5e with the crystal structure of GlcN-6-P synthase (ID: 1MOQ). According to the docking results, complex 2b exhibited a highest score (8,882; ACE = -580.43kcal/mol) that is well correlated with a high inhibition as compared to other complexes which corresponds to the antibacterial screening outcomes.

Intersectional Hybrids between Darrow's Blueberry (V. darrowii Camp) and Lingonberry (V. vitis-idaea L.).

An initial cross of V. darrowii 'Johnblue' (Darrow's blueberry) × V. vitis-idaea 'Red Sunset' (lingonberry) produced more than 30 true intersectional diploid hybrids as confirmed by molecular markers. The most vigorous of these hybrids was extensively evaluated. This hybrid, US 2535-A, was floriferous and morphologically intermediate to the respective parents. Examination of pollen suggested low male fertility. Numerous crosses using the hybrid as a female reflected similarly low fertility and potential crossing barriers. Stylar examination suggested blockage of pollen tube growth in self-pollinations and significantly retarded growth in backcross pollinations. Nonetheless, two confirmed hybrid offspring were produced using the F1 hybrid as a female in crosses with V. vitis-idaea and V. darrowii, respectively. In a second set of crosses utilizing additional V. darrowii and V. vitis-idaea genotypes, another 23 verified hybrids in seven parental combinations were produced. Hybrids such as the ones presented offer the potential for generating de novo interspecific fruit types in blueberry and/or broadening the adaptation of lingonberry.

Comparison of Religious Coping Strategies in Taiwanese and Turkish Parents of Children with Cancer

<p>在本文中我們討論並比較了生活在兩種不同文化中的台灣和土耳其癌症兒童父母的宗教因應策略，以引起人們對人類經驗的宗教/靈性維度的重要性的關注。癌症是兒童疾病相關死亡和治療相關的致病率的主要原因之一，近幾十年來，癌症在全球呈上升趨勢。宗教因應是一種以與神聖相關的方式理解和處理生活壓力的努力，包括祈禱、會眾支持、教牧關懷和宗教信仰。台灣和土耳其的癌症兒童父母經常使用宗教因應方式，因為宗教對心理靈性有好處；然而，由於父母的宗教信仰和習俗不同，所使用的宗教因應策略也大不相同。大多數台灣父母的信仰是傳統民間信仰和道教，而大多數土耳其父母的信仰是伊斯蘭教。我們堅信，醫療專業人員應該認識並尊重父母的宗教和靈性需求，並鼓勵父母採用宗教因應策略。</p> <p> </p><p>In this article, we discussed and compared the religious coping strategies of Taiwanese and Turkish parents of children with cancer, living in two different cultures, in order to draw attention to the importance of the religious/spiritual dimension of human experience. Cancer is one of the leading causes of disease-related death and treatment-associated morbidity in children with an increasing trend in recent decades worldwide. Religious coping is an effort to understand and deal with life stress in ways related to the sacred, which includes prayer, congregational support, pastoral care, and religious faith. Religious coping has been frequently used by Taiwanese and Turkish parents of children with cancer because it brings psychospiritual benefits; however, the religious coping strategies used are largely different since the parents’ religious beliefs and practices are different. While the beliefs of most Taiwanese parents are traditional folk beliefs and Taoism, the belief of most Turkish parents is Islam. We strongly believe that healthcare professionals should recognize and respect parents’ religious and spiritual needs and encourage parents to use religious coping strategies.</p> <p> </p>

Marker-assisted introgression lines of elite Indian mustard (Brassica juncea) cultivars for resistance against white rust (Albugo candida)

White rust caused by Albugo candida is a major biotic constraint responsible for significant yield loss in mustard production across all mustard-growing regions. This experiment was conducted with the aim of developing WRR lines of Indian mustard using the MABB approach. The elite mustard cultivars of Brassica juncea viz., DRMR150-35, DRMRIJ-31 and NRCDR-02 were used as the recurrent parent, whereas BioYSR, BEC-144 and Donskaja were taken as donors. A parental polymorphism survey using 315 microsatellites (SSR) was done among recurrent and donor parents. To confirm introgression of white rust resistance gene foreground selection was performed using Arabidopsis-derived IP markers that are linked to the white rust loci AcB1-A5.1 (At2g36360) for Donskaja and BioYSR and AcB1-A4.1 (At5g41560) for BEC-144coupled with background selection and phenotypic selection for highest recurrent parent genome recovery. Finally, 19 BC3F3 lines were obtained, which led to the identification of 5 highly white rust-resistant lines with RPG recovery percentage of ˃90%. These selected improved lines showed a resistance response with mean %WR severity (100 DAS) below 5% and possess the agro-morphological characters at par to their respective recurrent parent. These lines have good potential for future release and demonstrated MABB as a valuable tool for expediency, enrichment and precision in accelerating the development of new disease-resistant Indian mustard varieties.

Improvement in yield attributes and fatty acids composition in the derivative hybrids compared to their respective parents in Indian mustard (Brassica juncea L.)

Erucic acid, more than 2 %, in mustard seed oil is considered unhealthy as edible oil, and also anti-nutritional for human consumption. The existing mustard varieties of Bangladesh contain 40–48 % erucic acid, which is a big concern for the country's nutritional, and food security and safety. Hence, to improve the seed oil quality of the existing variety, six popular cultivars of Brassica juncea mustard were crossed with a canola-grade line in 7 × 7 half diallel fashion, and the developed 21 F1 hybrids were assessed for yield contributing traits, and fatty acids composition. Variables with significant variations were found, while days to siliquae maturity, plant height, days to first flowering, and seeds per siliquae have moderate narrow sense heritability. The estimated gene action indicated that dominant or over-dominant gene action was more prominent in governing the traits. The parents, P1, P3, and P4 were discovered the best general combiners for early maturity and short phenology, whereas P2 and P7 were found to be the best general combiners for yield-attributing traits. Moreover, the hybrids P1 × P4, P1 × P6, P2 × P7, P4 × P6 and P3 × P5 were chosen as the promising hybrids due to their best specific combining ability, and desired heterotic effects on yield contributing traits. In addition, a significant decrease, on average 30–40 %, in erucic acid, but an approximately 20–25 % increase of oleic acid was found among the hybrids, in which the hybrids P1 × P6–S1, P5 × P6–S2 and P5 × P6–S4 demonstrated a better stability index. Overall, the obtained findings suggested that the hybrids, viz. P1 × P5, P1 × P6, P2 × P3, P2 × P7, P4 × P6, P5 × P6, and P6 × P7 were promising based on their early maturity, high-yielding, reduced erucic acid, and high oleic acid contents.

The processes and outcomes related to 'family-centred care' in neuromotor and functional rehabilitation contexts for children with cerebral palsy: A scoping review.

The aim of this study is to identify the main processes and outcomes related to family-centred care (FCC) in neuromotor and functional rehabilitation of preschool children with cerebral palsy (CP). FCC is considered a reference for best practices in child rehabilitation. CP is the most common cause of physical disability in childhood with repercussions on functionality. There is a gap in knowledge of the practical principles of FCC, and it is necessary to develop a reference model for the practice of child rehabilitation professionals. In this scoping review, the main databases selected were as follows: LILACS; Pubmed; Embase; The Cochrane Library; CINAHL (EBSCO); Scopus; Web of Science; PEDro (Physiotherapy Evidence Database); Open Gray and other banks of thesis. The terms combined in the search strategy were as follows: 'Family-centered', 'Family-centred' and 'CP'. Inclusion criteria are as follows: studies on preschool-aged children with CP, undergoing family-centred functional therapeutic interventions (FCFTI) with outcomes on bodily structures and functions and/or activities and/or participation. The main participatory care methods identified were home intervention, environmental enrichment, collaborative realistic goal setting, planning of home-based activities and routine, child assessment feedback, family education/training, family coaching, encouraging discussion, observation of therapist and supervised practice. The main relational care qualities identified were as follows: respect, active listening, treat parents as equals, clear language, respect parents' ability to collaborate, demonstrate genuine care for the family, appreciate parents' knowledge and skills, demonstrate competence, experience and commitment. The main outcomes identified in children were improvement in motor and cognitive function and the child's functional ability. The main parentaloutcomes identified were empowerment, feeling of competence, self-confidence, motivation and engagement. The main differences in FCFTI programs refer to the parental education/guidance component and the amount of intervention carried out by parents. It is possible that the elements chosen by the therapist in a FCFTI depend on characteristics of the child and caregivers.

Proximal sensors for modeling clay mineralogy and characterization of soil textural fractions developed from contrasting parent materials

Proximal sensors combined with X-ray diffraction (XRD) have optimized soil characterization, but scarce studies have focused on predicting the contents of minerals under this scope. The objectives herein were to: a) use the portable X-ray fluorescence (pXRF) spectrometry, diffuse reflectance spectroscopy in the range of visible and near-infrared (Vis-NIR), magnetic susceptibility (χ) and XRD to characterize the mineralogy of soils derived from representative Brazilian soil parent materials, and b) create models to quantify the minerals obtained via XRD. Twenty-two soil profiles developed from gabbro, gneiss, quartzite, mineral and organic sediments were described with 53 soil horizons sampled. Each sample had the sand, silt and clay fractions separated and analyzed with XRD, pXRF, χ, and Vis-NIR. Models were created using the Random Forest algorithm permuting the following predictor variables (separately and combined): pXRF, parent material (PM), χ, soil texture (sand, silt, and clay content), and Vis-NIR. Models’ accuracy was calculated using the leave-one-out cross-validation method. Si, Al, Fe, Ca, K, and Ti contents obtained by pXRF and the χ discriminated the soil particle size fractions according to the parent material. XRD analysis allowed the evaluation of the pedogenetic development of soils and their relation to the respective parent material. The best models for mineral contents were found for hematite (Hm) (104)+(Gt) (130) (R2 = 0.85), Hm (110) + Mh (131) (R2 = 0.76), kaolinite (Kt) (001) (R2 = 0.73), Kt (002) (R2 = 0.80), mica (Mc) (001) (R2 = 0.77), and Mc (020) + Kt (020) (R2 = 0.81). Clay mineralogy content was accurately modeled using only pXRF and parent material data. This approach can facilitate and speed up detailed soil mineralogy characterization. Further studies are encouraged to model the content of minerals found in the sand and silt fractions of soils with diverse mineralogy via proximal sensors and using larger data sets.

Heterosis of endophytic microbiomes in hybrid rice varieties improves seed germination.

Seed endophytic microbiomes are shaped by host and environmental factors and play a crucial role in their host growth and health. Studies have demonstrated that host genotype, including hybridization, affects seed microbiomes. Heterosis features are also observed in root-associated microbiomes. It remains unclear, however, whether heterosis exists in seed endophytic microbiomes and whether hybrid microbiota provide noticeable advantages to host plant growth, especially to seed germination. Here, we investigated the structure of seed endophytic bacterial and fungal communities from three hybrid rice varieties and their respective parents using amplicon sequencing targeting 16S rRNA and ITS2 genes. Heterosis was found in diversity and composition of seed endophytic microbiomes in hybrids, which hosted more diverse communities and significantly higher abundances of plant growth-promoting taxa, such as Pseudomonas and Rhizobium genera compared with their parental lines. Co-occurrence network analysis revealed that there are potentially tighter microbial interactions in the hybrid seeds compared with their parent seeds. Finally, inoculation of seed-cultivable endophytes, isolated from hybrids, resulted in a greater promotion of seed germination compared with those isolated from parent lines. These findings suggest that heterosis exists not only in plant traits but also in seed endophytic microbiota, the latter in turn promotes seed germination, which offers valuable guidance for microbiome-assisted rice breeding.IMPORTANCEGenetic and physiological changes associated with plant hybridization have been studied for many crop species. Still, little is known about the impact of hybridization on the seed microbiota. In this study, we indicate that hybridization has a significant impact on the endophytic bacterial and fungal communities in rice seeds. The seed endophytic microbiomes of hybrids displayed distinct characteristics from those of their parental lines and exhibited potential heterosis features. Furthermore, the inoculation of seed-cultivable endophytes isolated from hybrids exhibited a greater promotion effect on seed germination compared with those isolated from the parents. Our findings make a valuable contribution to the emerging field of microbiome-assisted plant breeding, highlighting the potential for a targeted approach that aims to achieve not only desired plant traits but also plant-beneficial microbial communities on the seeds.

Application of machine learning for identification of heterotic groups in sunflower through combined approach of phenotyping, genotyping and protein profiling

Application of machine learning in plant breeding is a recent concept, that has to be optimized for precise utilization in the breeding program of high yielding crop plants. Identification and efficient utilization of heterotic grouping pattern aided with machine learning approaches is of utmost importance in hybrid cultivar breeding as it can save time and resources required to breed a new plant hybrid/variety. In the present study, 109 genotypes of sunflower were investigated at morphological, biochemical (SDS-PAGE) and molecular levels (through micro-satellites (SSR) markers) for heterotic grouping. All the three datasets were combined, scaled, and subjected to unsupervised machine learning algorithms, i.e., Hierarchical clustering, K-means clustering and hybrid clustering algorithm (hierarchical + K-means) for assessment of efficiency and resolution power of these algorithms in practical plant breeding for heterotic grouping identification. Following the application of machine learning unsupervised clustering approach, two major groups were identified in the studied sunflower germplasm, and further classification revealed six smaller classes in each major group through hierarchical and hybrid clustering approach. Due to high resolution, obtained in hierarchical clustering, classification achieved through this algorithm was further used for selection of potential parents. One genotype from each smaller group was selected based on the maximum seed yield potential and hybridized in a line × tester mating design producing 36 F1 cross combinations. These F1s along with their parents were studied in open field conditions for validating the efficacy of identified heterotic groups in sunflowers genetic material under study. Data for 11 agronomic and qualitative traits were recorded. These 36 F1 combinations were tested for their combining ability (General/Specific), heterosis, genotypic and phenotypic correlation and path analysis. Results suggested that F1 hybrids performed better for all the traits under investigation than their respective parents. Findings of the study validated the use of machine learning approaches in practical plant breeding; however, more accurate and robust clustering algorithms need to be developed to handle the data noisiness of open field experiments.

Development and Application of a Multidimensional Database for the Detection of Quaternary Ammonium Compounds and Their Phase I Hepatic Metabolites in Humans.

The COVID-19 pandemic has led to significantly increased human exposure to the widely used disinfectants quaternary ammonium compounds (QACs). Xenobiotic metabolism serves a critical role in the clearance of environmental molecules, yet limited data are available on the routes of QAC metabolism or metabolite levels in humans. To address this gap and to advance QAC biomonitoring capabilities, we analyzed 19 commonly used QACs and their phase I metabolites by liquid chromatography-ion mobility-tandem mass spectrometry (LC-IM-MS/MS). In vitro generation of QAC metabolites by human liver microsomes produced a series of oxidized metabolites, with metabolism generally occurring on the alkyl chain group, as supported by MS/MS fragmentation. Discernible trends were observed in the gas-phase IM behavior of QAC metabolites, which, despite their increased mass, displayed smaller collision cross-section (CCS) values than those of their respective parent compounds. We then constructed a multidimensional reference SQLite database consisting of m/z, CCS, retention time (rt), and MS/MS spectra for 19 parent QACs and 81 QAC metabolites. Using this database, we confidently identified 13 parent QACs and 35 metabolites in de-identified human fecal samples. This is the first study to integrate in vitro metabolite biosynthesis with LC-IM-MS/MS for the simultaneous monitoring of parent QACs and their metabolites in humans.

Nilai Profetik Dalam Novel Bidadari Bermata Bening Karya Habiburrahman El Shirazy dan Implikasi terhadap Pembelajaran Sastra di Madrasah Aliyah

This study aims to determine the prophetic values in the novel Bidadari Bermata Bening by Habiburrahman El Shirazy and Implications for Literature Learning in Madrasah Aliyah (MA). In this thesis, researchers use analytical techniques or qualitative descriptive methods. Descriptive qualitative is qualitative data in the form of words, schemes, and images. The process of data analysis is carried out by reading, analyzing the prophetic values in the novel Bidadari Bermata Bening by Habiburrahman El Shirazy. Based on data analysis, the results of analysis in the aspect of prophetic values were found through conversations and narratives in the novel Bidadari Bermata Bening by Habiburrahman El Shirazy, it is known that there are 3 (three) elements of prophetic values in it, namely 1. Humanization (Amar Ma'ruf), 2. Liberation (Nahi Munkar), 3. Transcendence (Tu'minunna Billah). Researchers found 95 prophetic values in the novel Bidadari Bermata Bening by Habiburrahman El Shirazy including (39 values of humanization or amar ma'ruf, 26 values of liberation or nahi munkar, 30 values of transcendence or tu'minunna billah). The results of this study revealed the prophetic values in the novel Bidadari Bermata Bening by Habiburrahman El Shirazy which narrowed down into three aspects. First humanization, humanization in this study is to produce far hatred, always do good to anyone who hurts, respect and respect parents, care for others and always invite in terms of kindness. Both liberations, liberation in this study is related to the rejection of corruption, eradication from ignorance, acquittal from vile accusations or slander, and discussion of the entanglement of use in political matters. Third, transcendence in this study is concerned with acknowledging God's power and power with complete conviction that everything and all motion and action proceeds from Him. Keywords: Prophetic Value, Qualitative Descriptive, Clear Eyed Angel Novel

Toxicokinetics of homosalate in humans after dermal application: applicability of oral-route data for exposure assessment by human biomonitoring

Homosalate (HMS) is a UV filter used in sunscreens and personal care products as a mixture of cis- and trans-isomers. Systemic absorption after sunscreen use has been demonstrated in humans, and concerns have been raised about possible endocrine activity of HMS, making a general population exposure assessment desirable. In a previous study, it was shown that the oral bioavailability of cis-HMS (cHMS) is lower than that of trans-HMS (tHMS) by a factor of 10, calling for a separate evaluation of both isomers in exposure and risk assessment. The aim of the current study is the investigation of HMS toxicokinetics after dermal exposure. Four volunteers applied a commercial sunscreen containing 10% HMS to their whole body under regular-use conditions (18–40 mg HMS (kg bw)−1). Parent HMS isomers and hydroxylated and carboxylic acid metabolites were quantified using authentic standards and isotope dilution analysis. Further metabolites were investigated semi-quantitatively. Elimination was delayed and slower compared to the oral route, and terminal elimination half-times were around 24 h. After dermal exposure, the bioavailability of cHMS was a factor of 2 lower than that of tHMS. However, metabolite ratios in relation to the respective parent isomer were very similar to the oral route, supporting the applicability of the oral-route urinary excretion fractions for dermal-route exposure assessments. Exemplary calculations of intake doses showed margins of safety between 11 and 92 (depending on the approach) after single whole-body sunscreen application. Human biomonitoring can reliably quantify oral and dermal HMS exposures and support the monitoring of exposure reduction measures.