Fasciolosis is a helminthosis of the liver caused by trematodes of the genus Fasciola. It is an enzootic disease in several West African countries, it causes milk losses and significant seizures of livers in slaughterhouses. The objective of this study is to determine the parasitic status of cattle slaughtered in slaughterhouses in relation to fasciolosis. It was carried out in 3 slaughterhouses in Mali, namely the Frigorifique Abattoir of Bamako (AFB), the Ségou Regional Abattoir (ARS) and the Mopti Regional Abattoir (ARM). A total of 900 faecal samples were analyzed by the successive wash sedimentation technique and 900 slaughtered cattle livers were inspected for Fasciola gigantica. When analyzing the data,the highest prevalences (feces and livers) were observed at the regional slaughterhouse of Mopti (13% and 30%), followed by the regional slaughterhouse of Ségou (11.5% and 18.5%). The lowest, at the Bamako refrigerated slaughterhouse (6.4% and 13.6%). It also emerges from the results obtained that overall the prevalences both for coprology and liver inspection, of the rainy season (11.3% and 20.3%) were higher than those of the dry season (6, 8% and 15.7%). In relation to the sexes, the study showed that females were more parasitized than males with coprological prevalences and infestation rates of 11.7% and 20.2% respectively in females compared to 7.2% and 17 % in males.