This study reports the concentrations of organic pollutants, nutrients, and some physicochemical parameters in water samples from Vlora Port. Organochlorine pesticides (OCPs), polychlorinated biphenyls (PCBs), polycyclic aromatic hydrocarbons (PAH) and BTEX (Benzene, Toluene, o-, m- and p-Xyelenes, Ethylbenzene) were analyzed organic pollutants. The levels of NO3-, NO2-, NH4-, N-total, PO4-, and P-total were determined. Temperature, PH, Conductivity, Turbidity, TDS, TSS DO, BOD and COD were physical-chemical parameters determined in the water samples. Vlora Port is located in the southern part of Albania in Vlora Bay, near Vlora City in the Adriatic Sea. Elevated activities in port areas (terrestrial and marine areas) are the main causes of water pollution. Urban pollution, agriculture, and water currents can influence marine water pollution in this area. To evaluate pollution levels at the port of Vlora, water samples (12 stations) were analyzed over a two-year period (2022 – 2023). Water samples were collected during two seasons: March and July of each year Analyses of organic compounds were performed using a gas chromatograph (Varian 450 GC) equipped with ECD and FID detectors. Nutrients were analyzed using the UV-VIS technique. Titration, automatic, semi-automatic, and gravimetric methods were used to determine the physicochemical parameters. Organic pollutants were detected in all the water samples. Nutrient concentrations and physicochemical values classified the marine water of Vlora’s port as moderately good. Levels of organic pollutants, nutrients, and physicochemical parameters in water samples from Vlora’s port were higher/comparable with those reported in previous studies of the Adriatic Sea (Albania coastline).