Metamaterials are artificially engineered structures that have unique properties not usually found in natural materials, such as negative refractive index. Conventional interferometry or ellipsometry is generally used for characterizing the optical properties of metamaterials. Here, we report an alternative optical vortex based interferometric approach for the characterization of the effective parameters of optical metamaterials by directly measuring the transmission and reflection phase shifts from metamaterials according to the rotation of vortex spiral interference pattern. The fishnet metamaterials possessing positive, zero and negative refractive indices are characterized with the vortex based interferometry to precisely determine the complex values of effective permittivity, permeability, and refractive index. Our results will pave the way for the advancement of new spectroscopic and interferometric techniques to characterize optical metamaterials, metasurfaces, and nanostructured thin films in general.