Parameters Of Forensic Importance Research Articles

Phylogenetic analysis and haplotype diversity in Christian residents of Lahore, Pakistan, using 17 Y-chromosomal STR loci.

This population data pertains to 250 unrelated male residents of the Christian population of Lahore, Pakistan. AmpF/STR®Yfiler PCR amplification kit was utilized for evaluation of 17 Y-chromosomal STRs loci. Ancestral lineages and parameters of forensic importance were examined leading to recognition of 135 unique out of 175 total haplotypes with diversity value of 0.991. All Y-STRs portrayed high discrimination power where DYS385 depicted the maximum value of 0.917. Multidimensional Scaling (MDS) and Analysis of Molecular Variance (AMOVA) were generated using YHRD (Y-Chromosome STR Haplotype Reference Database) tools. A pair-wise genetic distance comparison using Rst, Fst, and associated p values lead to the conclusion that studied Christian community was significantly demarcated from the rest of the global populations.

Optimization of a reduced volume PCR amplification for PowerPlex® Fusion kit using FTA™ cards and generation of population genetic data for Brunei population.

The commercial PowerPlex® Fusion kit is an autosomal STR multiplex kit that has high discrimination power and is more informative in forensic, paternity, and relationship-testing cases. Key features of this multiplex system are the possibility to direct amplify FTA™ card punches as well as non-FTA cards and commonly used swabs; optimised inhibitor tolerance and high sensitivity generating full profiles from amount as little as 100pg of human DNA. This study focused on the optimization of performance variables such as FTA™ punch sizes, reduced reaction volumes, and FTA™ purification reagent aiming to increase the analytical sensitivity, decrease the sample consumption, and cost effectiveness. LOD and LOQ values demonstrated high sensitivity of the PowerPlex® Fusion system. In addition, population databases of Brunei Malay and Chinese from the Brunei Darussalam were established, and parameters of forensic importance were calculated. Overall, the forensic parameters indicated an enhanced utility of the PowerPlex® Fusion kit for forensic evidence analysis and paternity testing in Brunei Malay and Chinese populations.

Forensic evaluation of 11 non-standard STR loci in Bangladeshi population

Allele frequencies and forensic efficiency parameters of 11 non-standard autosomal STR loci, D21S1437, D22S683, D8S1110, D10S2325, D12S1090, D17S1294, D3S1744, D14S608, D20S470, D18S536 and D13S765 were evaluated in a sample of 102 unrelated Bangladeshi individuals. No significant deviation from Hardy-Weinberg equilibrium was observed in any of the loci studied. Statistical parameters of forensic importance, the power of discrimination (PD), observed and expected heterozygosity (H), polymorphism information content (PIC), matching probability (MP), power of exclusion (PE) and typical paternity index (TPI) were calculated for the loci. The most informative locus among the 11 STR loci was D10S2325 (PD=0.958), while the least informative was D17S129 (PD=0.876). The combined PD (1-PM) and MP was calculated to be 0.9999999999997 and 2.23×10(-23), respectively. These parameters indicated the usefulness of the loci in forensic personal identification and parentage testing among Bangladeshi population.

Analysis of 16 Y-chromosomal STRs in an African descent sample population of Chocó (Colombia)

We studied and established a database and some parameters of forensic importance were calculated of 16 Y-STR (DYS19, DYS385, DYS389I/II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438, DYS439, DYS460, DYS461, GATA-A10, GATA-H4 and DYS635) in a population of 298 unrelated males of African descent of Chocó (Colombia) and a total of 257 haplotypes were identified using the present set of Y-STR markers, of which 224 were represented only once in the database. Twenty-six haplotypes were presents two times, six haplotypes were presents in three individuals and one haplotype in four men. The haplotype diversity was 0.9987 ± 0.0004. By combining the allelic states of the 16 Y-chromosomal STRs we could construct highly informative haplotypes that allowed the discrimination of 86.2% of the samples tested. With this work we established a database of Y-STR and some parameters of forensic importance. This approach represents a very powerful tool for individual identification and paternity testing in forensic genetic.

Usefulness of 12 Y-STRs for forensic genetics evaluation in two populations from Venezuela

The distribution of allele frequencies and haplotypes for 12 STRs loci, (DYS19, DYS389I/II, DYS390, DYS391, DYS392, DYS393, DYS385a/b, DYS437, DYS438 and DYS439) on the Y-chromosome from two Venezuelan populations were determined in 173 DNA samples of unrelated males living in Caracas (62) and Maracaibo (111). Some parameters of forensic importance were calculated. AMOVA and genetic distances between these populations were estimated. The results confirmed Y-STR genotypes as useful markers for forensic genetics analysis.

Analysis of 16 Y-chromosomal STRs in a Córdoba (Colombia) population sample

We studied and established a database of 16 Y-STR (DYS19, DYS385, DYS389I/II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438, DYS439, DYS460, DYS461, GATA-A10, GATA-H4 and DYS635) in a population sample of 123 unrelated males of Córdoba (Colombia) and some parameters of forensic importance were calculated. The haplotype diversity was 0.9987. This approach represents a very powerful tool for individual identification and paternity testing in forensic medicine.

Analysis of 16 Y-chromosomal STRs in a Valle (Colombia) population sample

We studied and established a database of 16 Y-STR (DYS19, DYS385, DYS389I/II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438, DYS439, DYS460, DYS461, GATA-A10, GATA-H4 and DYS635) in a population sample of 150 unrelated males of Valle (Colombia) and some parameters of forensic importance were calculated. One hundred forty-six different haplotypes were found, 142 haplotypes of them were found to be unique and the others were shared by two persons. The haplotype diversity was 0.9996. The AMOVA results show that the percentage of variation is mainly within populations (99.95%) in agreement with previous results in European populations. By combining the allelic states of the 16 Y-chromosomal STRs, we could construct highly informative haplotypes that allowed the discrimination of 97.3% (146 out of 150) of the samples tested. This approach represents a very powerful tool for individual identification and paternity testing in forensic medicine.

Analysis of 16 Y-chromosomal STRs in a Cartagena (Colombia) population sample

We studied and established a data base of 16 Y-STR (DYS19, DYS385, DYS389I/II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438, DYS439, DYS460, DYS461, GATA-A10, GATA-H4 and DYS635) in a population of 173 unrelated males of Cartagena (Colombia) and some parameters of forensic importance were calculated. The haplotype diversity was 1.000±0.0006. This approach represents a very powerful tool for individual identification and paternity testing in forensic medicine.

Population Frequencies for CSF1PO, TPOX, TH01, F13A01, FES/FPS and VWA in Seven Amerindian Populations from Colombia

POPULATION: The Amerindian population of Colombia represents around 1.7% of the population (1,2). They belong to different linguistic families (Chibcha, Tukano, Arawak, Guahibo, Choco, Maku, Karib, and Quechua among others). The Amerindian tribes of Colombia are located in the Amazonian/Orinoquian region, the Northeast, Southwest and Pacific regions of Colombia. Few studies have been carried out for STR variability among Amerindian tribes (3-5). On the other hand, several studies have been performed for STR variability in Caucasian and Afro-Colombian populations (1,2,6-10). We have carried out a study in 7 Amerindian populations named Cubeo (n = 49), Curripaco (n = 32), Desano (n = 28), Tucano (n = 26), Embera (n = 31), Puinabe (n = 77), and Nukak (n = 29) for 6 STR loci (CSF1PO, TPOX, TH01, F13A01, FES/FPS and VWA) to determine allele frequencies and other parameters of forensic importance.