Community Structure Parameters Research Articles

Spatial and Temporal Analysis of the Relationship between Phytoplankton Community Structure and Environmental Parameters of Yuvacık Reservoir

Spatial and Temporal Analysis of the Relationship between Phytoplankton Community Structure and Environmental Parameters of Yuvacık Reservoir

Seasonal Variations in Ostracod Community Structure and Physicochemical Parameters in Khanki Headworks, Pakistan

Protozoans, rotifers, ostracods and copepods are principal groups of zooplanktons. These organisms are crucial biotic elements, and impact all functional features of aquatic ecosystems. Objective: To assess the impacts of environmental variables on the abundance, biodiversity and seasonal distribution of ostracods in Khanki Headworks, Pakistan. To evaluate the contribution of ostracods as bio-monitors of contamination in freshwater ecosystems. Methods: Month wise separate water samples were taken for different environmental variables from four sampling sites (each with 3 sub-sites), for study period of one year (February 2021 to January 2022), whereas, samples of microcrustaceans (ostracods) were collected with planktonic net (mesh size: 37µm). Results: In total, 5 species of ostracods, associated to 4 genera and 2 families were identified from February 2021 to January 2022. Heterocypris incongruence was the most abundant species, whereas, Dolerocypris sinensis was noted as least abundant species. Ostracods abundance and biodiversity were monitored highest in June and minimum in the month of January. Results of Pearson correlation showed that physicochemical parameters, electrical conductivity (EC), temperature, turbidity, total dissolved solids (TDS) and pH have positive effects on the density and diversity of ostracods. In contrast, dissolved oxygen (DO) showed negative relationship with the abundance and diversity of planktonic ostracods. These findings were further validated by Canonical correspondence analysis (CCA). Conclusions: This investigation revealed that physicochemical parameters generally govern the population dynamics of ostracods.

Dynamics of the natural enemy community of Hyphantria cunea (Lepidoptera: Erebidae) in Dandong, China.

This study aims to explore the composition of natural enemy species in the fall webworm, Hyphantria cunea (Drury) population and the dynamics of its natural enemy community in Dandong, Liaoning Province, China, where it was first reported. We collected the natural enemy of eggs, larvae, and pupae of H. cunea on host trees at 12 survey sites from June 2019 to October 2020. The results showed that the community consists of 34 species: 20 predatory species, including 15 spiders and 5 insects, and 14 parasitic species, including 10 parasitic wasps and 4 parasitic flies. The top 3 dominant species based on the importance value index for both parasitic and predatory species were Pediobius pupariae > Chouioia cunea > Cotesia gregalis in the natural enemy community of H. cunea. Analysis of all 3 principal components by principal component analysis showed that Clubionidae sp. 1, Parena cavipennis, or other predators were the main factors affecting the natural enemy community. Analysis of the community structure parameters of the H. cunea natural enemy community in different developmental stages across generations revealed the following: (i) Compared with the degree of complexity of the egg and pupal stages, the larval stage was the highest. (ii) The complexity was determined by means of comprehensive evaluation: first-generation larvae in 2020 > first-generation larvae in 2019 > second-generation larvae in 2020 > second-generation larvae in 2019. These results clarify the dynamics of natural enemy species, coevolution with the host in the invaded habitat of H. cunea and development of biological control technologies.

Macroinvertebrate community structure, pollution tolerance, diversity and feeding functional groups in polluted urban rivers under different black and odorous levels

Macroinvertebrate community structure parameters can reflect the water quality and water ecosystem health status of rivers. In this paper, using the results of macroinvertebrates in 16 urban rivers in Sichuan Province from November 2020 to March 2021, we independently evaluated the level of black odor in urban rivers based on the mathematical model. On this basis, we examined the community structure, fouling tolerance, diversity, and feeding functional groups of macrobenthos in rivers with different degrees of black odor. During the study, a total of 39 species of macroinvertebrates were collected and identified to 3 phyla, 7 classes, 16 orders, 26 families and 30 genera. Urban rivers changed from non-black and non-odorous to black and odorous: (1) the dominant species, Chironomus flaviplumus, Physa fontinalis and Barbronia weberi, changed to Chironomus flaviplumus, a fouling-tolerant species with wide adaptability; (2) According to the tolerance values (TVs), the pollution resistance categories gradually increased as the level of black odor increased, which indicated the level of black odor in water bodies; (3) The calculation of the Hilsenhoff biotic indices (HBI) indicated that 83.3% of the sampled sites were moderately to heavily polluted. In general, the severity of water quality pollution in the black and odorous type of river was significantly higher than that in the non-black and odorous type of river, and with the deepening of the degree of blackness and odor the quality of the water deteriorated; (4) The community structure had an unstable community structure and a low diversity index of macroinvertebrate species; (5) The taxa of feeding functional groups shifted from the presence of all five types of feeding functional groups to the dominance of fouling-resistant species represented by scrapers. The results summarize the characteristics and change patterns of various indicators under different black-odorous degrees, indicating that the HBI can be used as an alternative indicator for the evaluation of black odorous water, and the number of species of Chironomidae can indicate the degree of black odor in water bodies.

Patterns of deep fine root and water utilization amongst trees, shrubs and herbs in subtropical pine plantations with seasonal droughts.

Seasonal droughts will become more severe and frequent under the context of global climate change, this would result in significant variations in the root distribution and water utilization patterns of plants. However, research on the determining factors of deep fine root and water utilization is limited. We measured the fine root biomass and water utilization of trees, shrubs and herbs, and soil properties, light transmission, and community structure parameters in subtropical pine plantations with seasonal droughts. We found that the proportion of deep fine roots (below 1 m depth) is only 0.2-5.1%, but that of deep soil water utilization can reach 20.9-38.6% during the dry season. Trees improve deep soil water capture capacity by enhancing their dominance in occupying deep soil volume, and enhance their deep resource foraging by increasing their branching capacity of absorptive roots. Shrubs and herbs showed different strategies for deep water competition: shrubs tend to exhibit a "conservative" strategy and tend to increase individual competitiveness, while herbs exhibited an "opportunistic" strategy and tend to increase variety and quantity to adapt to competitions. Our results improve our understanding of different deep fine root distribution and water use strategies between overstory trees and understory vegetations, and emphasize the importance of deep fine root in drought resistance as well as the roles of deep soil water utilization in shaping community assembly.

Gastropod community structure in the water of Terkulai Island Tanjungpinang City

Gastropods are marine biota found in the waters of Pulau Terkulai, Tanjungpinang City and are one of the catch of fishermen. However, information on the structure of the gastropod community in these waters is not widely known. The purpose of this study was to determine the types of gastropods, gastropod community structure, and the relationship between gastropod community structure and environmental parameters in the waters of Terkulai Island. This research was conducted in April-August 2023. Determination of sampling points using random sampling method as many as 30 points with sampling plots measuring 1x1m2. The results of this study obtained 650 individuals with 15 species of gastropods. The highest density was found in Batillaria attramentaria species with a total of 12.20 ind/m2. Gastropod community structure diversity value (H') ranged from 1.512 which is classified as moderate. The uniformity value (E) ranges from 0.558 which is classified as moderate and the dominance value (D) ranges from 0.353 which is classified as moderate. The results of the measurement of the physicochemical parameters of the waters are still at a feasible threshold for gastropod life. The principal component analysis (PCA) shows that the density with water parameters has a positive correlation with temperature and organic matter.

How does land-use change alter soil microbial diversity, composition, and network in subtropical China?

Soil microbial communities play essential roles in degraded soil ecosystems. The “emergent trait” indices (e.g., microbial biomass C and N, and enzyme activity) and specific soil microbiota associations have been intensively studied, but the responses of soil microbial composition and network complexities to land-use changes remain unclear. Using amplicon sequencing of 16S rRNA genes and internal transcribed spacer (ITS) regions, we assessed the response ratio of soil microbial genomic diversity, community structure, and network parameters to primary evergreen broadleaved forest (control) conversion to conifer plantations, orchards, sloping tillage, and paddy fields. Agricultural lands had a higher soil bacterial and lower fungal diversity than primary forest. Overall, primary forest conversion reduced microbial network complexity, except in the sloping tillage. Moreover, land-use changes shifted the microbial community composition by either decreasing or increasing the relative abundance of dominant and putative keystone associations. The mean response ratio (indicator response percentage to primary forest conversion) of the microbial index to the land-use change varied depending on the soil microbial alpha diversity, network topological parameters, and dominant and putative keystone associations. Alterations in soil microbial diversity, composition, and network complexity were related to reduced soil resource availability following land-use change. Our study suggested that primary forest conversion to agricultural lands did not always negatively influences soil microbial diversity, composition, network complexity, and putative keystone associations. In addition, specific soil microbial associations showed highly sensitive to land-use change.

Bacterial Community Structure, Diversity, and Fertility of Soil with and without Press Mud in Two Sites in Panay, Philippines

Sugarcane farmers commonly use press mud as organic fertilizer to improve the properties of soil and increase crop production. This study compared the effects of press mud on bacterial community structure, bacterial diversity, and physicochemical parameters of sugar mill soil in two sites, the URC Passi Sugar Central and Passi Sugar Central, Inc. both in Panay, Philippines. DNA and soil analyses were conducted to characterize the soil samples with and without press mud from both sites. The results showed that all nutrient contents increased in both areas after press mud application, except in URC Passi Sugar Central, where no increase in phosphorus and sulfur was observed. Bacterial diversity did not significantly increase six months after press mud application. Community pattern results showed that if soil samples within sites were compared, soil with press mud was significantly higher compared to the soil without press mud in terms of OTU richness (Capiz sugar central: 46.00±1.00, 40.00±1.50, respectively; URC Passi Sugar Central: 48.00±0.50, 45.00±0.00, respectively) and carrying capacity (Capiz sugar central: 169±7.36, 125±9.48, respectively; URC Passi Sugar Central: 181±3.80, 162±0.00, respectively) (p<0.05). The same trend was observed in soil samples between sites that were compared. Soil without press mud from URC Passi Sugar Central had a significantly higher compared with soil without press mud of Capiz Sugar Central in terms of OTU richness (45.00±0.00, 40.00±1.50, respectively) and carrying capacity (162±0.00, 125±9.48, respectively) (p<0.05). However, no significant difference was observed in soil with press mud between the two sites in OTU richness, carrying capacity, Shannon, and Evenness analyses (p>0.05). The phylogenetic tree analysis showed that Massilia sp. is closely related to Burkholderia arboris, and Lysobacter sp. is closely related to both Massilia sp.and B. arboris. Generally, press mud application helps increase the available nutrients, diversity, and community patterns in soil, making it good organic fertilizer.

Influence of seasonality, wastewater treatment plant process, geographical location and environmental parameters on bacterial community selection in activated sludge wastewater treatment plants treating municipal sewage in South Africa

This is the first comprehensive study that focusses on the correlation between the bacterial community composition and a range of previously identified selective criteria in activated sludge wastewater treatment plants on the African continent. Multivariate statistical analyses were used to determine the relative significance of the geographical location (factor: site), wastewater treatment plant process (factor: configuration), seasonality (factor: season), and environmental parameters on the bacterial communities in nine wastewater treatments plants from two sites in South Africa using terminal restriction fragment length polymorphism as a screening tool to rationalize the number of samples (to 50 samples) for high throughput (Illumina MiSeq) sequencing.Site was the most significant factor (Global ANOSIM R value = 0.91, p = 0.001), and it was established that the inter-site differences were not climatic in origin but related to differences in the composition of the influent and activated sludge. Previous studies that have reported associations between microbial community structure and environmental parameters have measured influent chemistry, and this is the first time, to our knowledge, that the comprehensive chemical character of activated sludge itself has been included in this type of study. It was found using BEST analysis that the activated sludge ammonia, activated sludge total phosphate and influent chemical oxygen demand were the most significant (p < 0.001) drivers for inter-site bacterial community selection (ANOSIM Global R values of 0.862, 0.782 and 0.428, respectively). This link would not have been established with only influent chemical analyses as there was no significant difference (t-test, p > 0.05) in the average influent phosphate concentrations between the 2 sites, but there was a highly significant difference (p < 0.001, t (15.5)>t-crit (2.01)) in the activated sludge total phosphate concentrations (20.8 ± 17.0 and 127.8 ± 40.2 mg/L). This is notable for all future studies on a global level aimed at identifying factors for selection of microbial communities in activated sludge.

The Species Structure of Plankton Communities as a Response to Changes in the Trophic Gradient of the Mouth Areas of Large Tributaries to a Lowland Reservoir

The mouth areas of large rivers can serve as a good model of heterogeneity sites with a pronounced trophic gradient to assess the impact of the degree of eutrophication on different plankton communities. The aim of this research was to identify the possible response of the diversity indicators of phyto- and zooplankton communities to trophic gradients in the mouth area of two large tributaries of the reservoir, formed in the Middle Volga River (Russia). Both linear regression models and canonical correlation analysis (CCA) were used to assess the role of abiotic and biotic predictors in the structural organization of plankton communities and to assess the changes in the parameters of the species plankton community structure in the trophic gradient. It was found that the species diversity (Adjusted R2 = 0.116) and evenness (Adjusted R2 = 0.114) of phytoplankton significantly decreased with an increase in the degree of eutrophication, while the species diversity (Adjusted R2 = 0.059) and evenness (Adjusted R2 = 0.073) of zooplankton increased. According to the CCA models, electrical conductivity (EC) explained the largest proportion of the observed dispersion. The Trophic State Index (TSI) explained 3.0% of the total variance in the phytoplankton community species structure and 7.8% in the zooplankton one. The variation in phyto- and zooplankton dominant complexes generally corresponded to the well-known patterns of plankton species succession in the gradient of trophic conditions and can be considered as a classic manifestation of the cascade effect in the food chains of freshwater plankton communities. Our results highlight the necessity of studying the mouth river areas, as well as applying an integrated approach to investigating the response of plankton communities to eutrophication processes of continental water bodies.

Spatiotemporal distribution of size-fractioned phytoplankton in the Yalu River Estuary, China

ABSTRACT The grain size structure of phytoplankton has great influence on shellfish culture. The present study aimed to assess the spatial and temporal variation in the phytoplankton community structure in the Yalu River Estuary and to explore the relationship between the phytoplankton community structure and various environmental parameters in 2020. High-throughput sequencing was used in this study. The results showed that nanophytoplankton, especially Karlodinium veneficum, dominated the estuary throughout the year. The biomass ratio of picophytoplankton, nanophytoplankton, and microphytoplankton were 20:63:17 in spring, 30:44:26 in summer, 1:38:61 in autumn, and 2:45:53 in winter, respectively. Meanwhile, Dinophyta had the greatest biomass throughout the year, followed by Bacillariophyta. On the spatial dimension (Station average), COD, T, SST had a positive impact on total phytoplankton communities, and Dep had a negative impact. In the time dimension (Monthly average), the environmental factor that significantly controlled the phytoplankton community structure were NO2 and SST.

Flow predictability indicates the ecological quality of the river: A case of invertebrates in Central Europe

• Predictability described most of macroinvertebrate structure parameters variation. • There was strong positive relation between BMWP_PL and flow predictability. • The regionalization is helpful tool for the rational management of water resources. • The predictability estimates the human impact on rivers ecological status. Flow regime and its variability play a fundamental role in preserving rivers' ecosystems. Effects of flow predictability modeling on macroinvertebrate community structure parameters and taxonomic composition were investigated. The main aim of the present work was to apply flow predictability as an indicator of river ecological health and to investigate the relationship between the flow predictability index and macroinvertebrate assemblages variation. Due to the high spatial variability in the abiotic and biotic characteristics of rivers, the classification and regression trees algorithm in respect to Polish Biological Monitoring Working Party (BMWP_PL) index on regional scale was done. The study was conducted in 30 catchments representing various rivers (from 0 m a.s.l. at the Baltic Sea, up to 2500 m a.s.l. in the Tatra Mountains) of Central Europe. Principal component analysis of macroinvertebrate habitat metrics indicated the presence of two groups of habitat characteristics. One of them is related to the quantitative characteristics of the regime, whereas is the second with the seasonality-predictability of the regime. Redundancy analysis showed that predictability of the hydrological regime was the most influential variables on macroinvertebrate community parameters. Generalized linear modeling showed that predictability (positively) and watershed area (negatively) influence on BMWP_PL index referred to the ecological statement of the river. Based on the main components analysis and CART algorithm in terms of the BMWP_PL index, analyzed catchments were divided into five groups according to predictability, high pulse durations, and catchments area. The predictability index is proposed as a good method for estimating the impact of human pressure on river biota without performing an extensive hydrobiological survey.

Mangroves Community Structure and Growth Parameters in Silo-Siloan Islet, Clarin, Bohol, Philippines

Mangroves Community Structure and Growth Parameters in Silo-Siloan Islet, Clarin, Bohol, Philippines

Effects of fishing and environmental parameters on the commercial bony fish assemblage in the southern Caspian Sea

Since the 1990s, anthropogenic activities have been the major direct or indirect threats to the structural and functional organization of the unique ecosystem of the Caspian Sea (CS). This study attempts to investigate the relationships between fish community structure and environmental parameters and to analyze the Abundance Biomass Comparison (ABC) in the CS. The input data, including catch data by species and environmental parameters, were collected from 1996 to 2017. Of the 13 bony fish species identified, only two species Rutilus kutum and Chelon aurata accounted for 47.14% and 62.65%, and 40.80% and 29.34% of CPUE and NPUE, respectively. DisTLM revealed that five environmental variables showed a significant linear relationship with the NPUE resemblance matrix. Based on the AIC criteria, the combination of year, precipitation, SST_Apr, SSL, SST, GTA, Iranian rivers, and Volga discharges explain 80.3% of the total variability. Based on the ABC curves and W statistics, it was concluded that the fish assemblage was environmentally stressed from 1996 to 2003. The Shannon diversity index (H´) showed a decreasing trend, suggesting that fish species have been exposed to increasing stress over the past 22 years. Considering the current environmental conditions (downward trend in SSL river discharge and increase in SST) and anthropogenic activities, it appears that the downward trend will continue in the future.

Variability of the Community Structure and Morphometric Parameters of Ground Beetles (Coleoptera, Carabidae) under an Anthropogenic Impact Gradient

Variability of the Community Structure and Morphometric Parameters of Ground Beetles (Coleoptera, Carabidae) under an Anthropogenic Impact Gradient

Diversity and Biogeography of Bathyal and Abyssal Seafloor Bacteria and Archaea Along a Mediterranean-Atlantic Gradient.

Seafloor sediments cover the majority of planet Earth and microorganisms inhabiting these environments play a central role in marine biogeochemical cycles. Yet, description of the biogeography and distribution of sedimentary microbial life is still too sparse to evaluate the relative contribution of processes driving this distribution, such as the levels of drift, connectivity, and specialization. To address this question, we analyzed 210 archaeal and bacterial metabarcoding libraries from a standardized and horizon-resolved collection of sediment samples from 18 stations along a longitudinal gradient from the eastern Mediterranean to the western Atlantic. Overall, we found that biogeographic patterns depended on the scale considered: while at local scale the selective influence of contemporary environmental conditions appeared strongest, the heritage of historic processes through dispersal limitation and drift became more apparent at regional scale, and ended up superseding contemporary influences at inter-regional scale. When looking at environmental factors, the structure of microbial communities was correlated primarily with water depth, with a clear transition between 800 and 1,200 meters below sea level. Oceanic basin, water temperature, and sediment depth were other important explanatory parameters of community structure. Finally, we propose increasing dispersal limitation and ecological drift with sediment depth as a probable factor for the enhanced divergence of deeper horizons communities.

Soil salinity and nutrients availability drive patterns in bacterial community and diversity along succession gradient in the Yellow River Delta

Exploring the main driving factors of microbial community in different succession stages of coastal wetland could provide insights into ecological restoration activities. The present study examined the soil microbial assemblage in a succession gradient of the coastal wetland in the Yellow River Delta (YRD) and elucidated the contributions of spatial structure, vegetation, and soil properties to microbial communities. Plant and soil samples in four succession stages from (S1, S2, S3, and S4) were selected in the coastal wetland of YRD. Soil bacterial community was analyzed using the 16S rRNA high-throughput sequencing technique. Partial mantel test and redundancy analysis were conducted to elucidate the relationship between bacterial community structure and environmental parameters. Soil pH and salinity decreased and soil nutrients increased with succession from mud flat to the late stage. Higher bacterial richness and diversity were also observed with coastal wetland succession. Although most of the soil properties, vegetation, and spatial distribution have an impact on the bacterial community, soil salinity and nutrient availability (e.g., total nitrogen content, nitrate content and dissolved organic carbon) have the greatest influence on the shift in the bacterial community along the succession gradient. Taken together, soil salinity and nutrients availability could be major drivers of variations in bacterial assemblage with coastal wetland succession. These findings improve our understanding of soil microbes as affected by environmental factors and microbial diversity could be considered as a helpful tool to evaluate the efficiency of ecological restoration in coastal wetlands.

Effect of submerged plant coverage on phytoplankton community dynamics and photosynthetic activity in situ

Restoration of submerged plants in eutrophic lakes can reduce nutrients and phytoplankton biomass in the water body. However, the effect of submerged plants on phytoplankton communities and their photosynthetic activity in situ are still poorly understood. Here, we studied the response of phytoplankton community structure and fluorescence parameters to different submerged plants coverage, the relationship of phytoplankton community and fluorescence parameters with submerged plants coverage and water physicochemical parameters were analysed in sampling area of Hangzhou West Lakes. The results showed that the coverage and biomass of submerged plants were negatively correlated with nitrogen and phosphorus contents in water body but positively correlated with total phenol content. The ratio of nitrogen to phosphorus in the study site changed greatly (32.25–124.54). In spring and summer, Oscillatoria and Leptolyngbya (Cyanophyta) were the dominant species, while in autumn and winter, the dominant species were Cyclotella (Chlorophyta), and Melosira and Cymbella (Bacillariophyta). Compared with Chlorophyta and Bacillariophyta, fluorescence parameters of Cyanophyta were more sensitive to total phosphorus, N:P ratio, total phenols, pH, and electric conductivity. Fluorescence parameters of Chlorophyta and Bacillariophyta were only affected by underwater light. Total phosphorus (TP) and N:P had a negative effect on the maximum photochemical electron yield of Cyanophyta. Furthermore, Cyanophyta was inhibited by total phenols from submerged plants. When phytoplankton were under stress, photochemical electron yield decreased significantly, whereas non-photochemical quenching increased. The structural equation model showed that the coverage of submerged plants might indirectly affect the fluorescence parameters of Cyanophyta by affecting nitrogen, phosphorus, and total phenol contents in the water body. These findings contribute to the understanding of the mechanisms underlying the impact of submerged plant restoration on phytoplankton community dynamics in subtropical eutrophic shallow lakes and provide a theoretical basis for the management of lakes.

Effect of the skincare product on facial skin microbial structure and biophysical parameters: A pilot study.

Daily use of cosmetics is known to affect the skin microbiome. This study aimed to determine the bacterial community structure and skin biophysical parameters following the daily application of a skincare product on the face. Twenty‐five Korean women, who used the same skincare product for four weeks participated in the study. During this period, skin hydration, texture, sebum content, and pH were measured, and skin swab samples were collected on the cheeks. The microbiota was analyzed using the MiSeq system. Through these experiments, bacterial diversity in facial skin increased and the microbial community changed after four weeks of skincare product application. The relative abundance of Cutibacterium and Staphylococcus increased, significant changes in specific bacterial modules of the skin microbial network were observed, and skin hydration and texture improved. It was suggested that daily use of skincare products could affect the microbial structure of facial skin as well as the biophysical properties of the facial skin. These findings expand our understanding of the role of skincare products on the skin environment.

Continuous Sugarcane Planting Negatively Impacts Soil Microbial Community Structure, Soil Fertility, and Sugarcane Agronomic Parameters.

Continuous planting has a negative impact on sugarcane plant growth and reduces global sugarcane crop production, including in China. The response of soil bacteria, fungal, and arbuscular mycorrhizae (AM) fungal communities to continuous sugarcane cultivation has not been thoroughly documented. Using MiSeq sequencing technology, we analyzed soil samples from sugarcane fields with 1, 10, and 30 years of continuous cropping to see how monoculture time affected sugarcane yield, its rhizosphere soil characteristics and microbiota. The results showed that continuous sugarcane planting reduced sugarcane quality and yield. Continuous sugarcane planting for 30 years resulted in soil acidification, as well as C/N, alkali hydrolyzable nitrogen, organic matter, and total sulfur content significantly lower than in newly planted fields. Continuous sugarcane planting affected soil bacterial, fungal, and AM fungal communities, according to PCoA and ANOSIM analysis. Redundancy analysis (RDA) results showed that bacterial, fungal, and AM fungal community composition were strongly associated with soil properties and attributes, e.g., soil AN, OM, and TS were critical environmental factors in transforming the bacterial community. The LEfSe analysis revealed bacterial families (e.g., Gaiellaceae, Pseudomonadaceae, Micromonosporaceae, Nitrosomonadaceae, and Methyloligellaceae) were more prevalent in the newly planted field than in continuously cultivated fields (10 and 30 years), whereas Sphingomonadaceae, Coleofasciculaceae, and Oxyphotobacteria were depleted. Concerning fungal families, the newly planted field was more dominated than the continuously planted field (30 years) with Mrakiaceae and Ceratocystidaceae, whereas Piskurozymaceae, Trimorphomycetaceae, Lachnocladiaceae, and Stigmatodisc were significantly enriched in the continuously planted fields (10 and 30 years). Regarding AMF families, Diversisporaceae was considerably depleted in continuously planted fields (10 and 30 years) compared to the newly planted field. These changes in microbial composition may ultimately lead to a decrease in sugarcane yield and quality in the monoculture system, which provides a theoretical basis for the obstruction mechanism of the continuous sugarcane planting system. However, continuous planting obstacles remain uncertain and further need to be coupled with root exudates, soil metabolomics, proteomics, nematodes, and other exploratory methods.