Brachiaria Mutica Research Articles

Four species of Chaetocnema Stephens, 1831 new to India (Coleoptera, Chrysomelidae, Galerucinae, Alticini)

Occurrence of four species of the flea beetle genus Chaetocnema Stephens viz. C. (Chaetocnema) kingpinensis Ruan, Konstantinov & Yang, C. (C.) yunnanica Heikertinger, C. (Udorpes) angustifrons Ruan, Konstantinov & Yang and C. (U.) reteimpunctata Ruan, Konstantinov & Yang are newly reported from India. Male of C. reteimpunctata is firstly described. Brachiaria mutica (Forssk.) Stapf (Poaceae) is newly recorded as the host plant of C. angustifrons.

Agronomic evaluation of 15 warm season grasses grown in temporarily waterlogged soils in Northeastern Argentina

Soils with temporarily waterlogged conditions are common in Northeastern Argentina. Fifteen accessions and hybrids of cultivated and native warm season grasses (Acroceras macrum, Urochloa species, Hemarthria altissima and Paspalum species) were planted in Basail, Chaco, in a completely randomized block design in November 2019. Initial, autumn and spring forage yield were measured in February 2020, July 2021 and December 2021, respectively. Plant height and plant diameter were evaluated in February 2020. Cold tolerance was estimated after the first frost in July 2021. Initial forage yield was between 29 and 181 g/plant. Urochloa mutica exhibited the greatest initial forage yield. Autumn and spring forage yield varied from 306 to 2,183 g/m2 and from 153 to 710 g/m2, respectively. U. mutica showed the highest autumn forage yield, however, it was the most damaged by cold weather. H. altissima hybrids were the most productive during spring and were cold tolerant. There were differences in plant height (7.5–49 cm) and plant diameter (68–268 cm) among the grasses. Grasses with prostrate, intermediate and upright growth habits were identified. Results indicate all grasses tested were adapted to humid Northeastern Argentina and varied in their morphological and agronomic traits.

Coupling of biochar and silicon for Phyto-management of Cd contaminated soil using Brachiariamutica

Contamination of soil with heavy metals is a threatening global environmental problem. With increasing urbanization, it is very important to design economical and environment-friendly mitigation measures to have pollutant free soil. Phytoremediation is an advancing technique to extract pollutants from soil to achieve eco-sustainability. This study aimed to investigate the combined impact of biochar and silicon on growth attributes of Brachiaria mutica grown in cadmium (Cd) contaminated soil. The biochar was prepared by co-pyrolysis of agricultural left-over biomass (rice husk, bamboo leaves and corn cob in a 1:1:1 ratio) and physio-chemical characters were studied by FTIR, SEM-EDX and proximate analysis. A pot study was conducted to evaluate the potency of biochar and silicon in lowering the Cd uptake by para grass grown under Cd spiked soil. Total 8 treatments were planned by taking 1 % and 2 % biochar with 0.01 % and 0.02 % silicon. The results have shown that treatment 8 (2 % biochar and 0.02 % silicon) exhibited maximum improvement in growth parameters such as shoot length by 2.64-fold and root length by 44.9 %, along with improving the antioxidant defense system of Para grass (CAT by 2-fold and POD by 56.4 %). Application of treatment 8 also decreased Cd concentration in the shoot and root by 48.8 % and 47.4 %, respectively. Treatment 8 was most effective in reducing CaCl2 extractable Cd in soil up to 56.1 %. Based on this data, treatment 8 was most effectual in suppressing Cd stress. Biochar produced from other agricultural waste material along with Si as inorganic amendment can be used to immobilize other toxic heavy metals at field level. Heavy metals immobilization potential of Si and biochar can be tested by harvesting other grasses and oil seed crop species.

Chemical Composition of Spent Mushroom Paddy Straw (SMPS) and its Nutritive Value in Cattle

Background: An experiment was conducted to study the chemical composition of spent mushroom (Pleuroyus ostreatus) paddy straw (SMPS) and its nutritive value in cattle. Samples of the SMPS were collected from various mushroom farms of Assam and were analysed for proximate principles according to the AOAC (2012) and fraction of fibre were estimated according to the Van Soest method (1991). Methods: Three digestion trials were conducted to evaluate the nutritive value of SMPS (difference method) by using a group of six mature healthy and non-productive cows of approximately same age and similar body weight. In first trial, only para grass was fed to animals, in second trial, para grass along with GNC were fed together and in third trial, SMPS and GNC were fed together to determine the digestibility for various nutrients of the SMPS. Result: The average chemical composition of SMPS were 42.65±1.33, 76.30±0.40, 7.23±0.24, 1.31±0.06, 21.25±0.76, 23.69±0.49, 46.51±0.57, 62.03±1.18 and 49.26±0.67 per cent of DM, OM, CP, EE, CF, TA, NFE, NDF and ADF, respectively. The DCP and TDN content of SMPS were 4.75 and 59.76 per cent, respectively. From the results obtained, it could be concluded that Spent Mushroom (Pleurotus ostroeatus) paddy straw had higher CP and total ash and lower CF and higher nutritive value (DCP and TDN) compared to paddy straw and could be used as a maintenance types of roughage source for feeding to cattle.

Potential interaction of metals tolerant bacterial strain (Bacillus cereus) with Para grass (Brachiaria mutica) for remediation of metal contaminated soil

BackgroundAnthropogenic activities are severely polluting the environment, particularly the soil. The quality of soil near the industrial area was investigated as well as the positive interaction between metal tolerance Bacillus cereus and Brachiaria mutica under greenhouse conditions. Furthermore, the PGP attributes of this B. cereus, as well as their impact on B. mutica growth and metal removal potential, were investigated. The soil quality parameters pH, EC, NPK, As, Cd, Cr, Fe, Mn, Pb, and Zn were also investigated. MethodsStandard methods were used to determine the physicochemical properties of the test soil sample. The metal tolerance and pollutant remediation potential of Bacillus cereus and Brachiaria mutica, as well as their positive interactions, were determined using the agar plate and greenhouse methods, respectively. FindingsMost of the soil parameters were found that were not meet the permissible standards. B. cereus possesses considerable tolerance to heavy metals (As, Cd, Mn, Cr, Fe, Pb, & Zn) up to 250 mg L-1 dosage. B. cereus also showed remarkable metal mobilization and metal absorption potential and significant PGP natures, which enhance the growth and essential biomolecule content as well as remediation potential of B. mutica.

Effect of Feeding Roughage Hay on Production Efficiency and Economic Return in Crossbred Sheep

This study aimed to investigate the effect of feeding roughage hay on production efficiency and economic return in crossbred sheep. A completely randomized design was employed on Santa Ines crossbred sheep from 16 animals and divided into 4 groups. The average initial body weights of all animals were 16.87±0.97 kilograms and the average final weight were 23.25±1.07 kilograms under 60 days feeding period. Four groups were fed with fresh grass (para grass) at 3.5 kg/head/day (T1; Control group), para grass hay at 1.5 kg/head/day (T2), ruzi grass hay at 1.5 kg/head/day (T3), and pangola grass hay at 1.5 kg/head/day (T4). All animals received concentrate with 14 %CP 0.2 kg/head/day throughout the period of the study. The results showed that animals in the 4 groups had no difference in weight gain and average daily gain (P>0.05). Total dry matter intakes of groups 1-4 were found to be 2.86±0.08, 1.28±0.09, 1.27±0.09 and 1.34±0.08 kgDM/head/day (P<0.01), respectively. Total dry matter intakes of protein were 0.09±0.01, 0.07±0.01, 0.08±0.01 and 0.10±0.01 kgDM/head/day, respectively (P<0.01). Total dry matter intake per body weight of group 1 was 16.99% of body weight, which was significantly higher than other groups (P<0.01). Blood metabolites such as blood glucose in range 63.94-70.04 mg/dl, and blood urea nitrogen in the range 13.44-17.13 mg/dl were found to be in the normal range (P>0.05). The total production costs per head of the four groups averaged 224.78±3.72, 240.42±3.73, 240.13±3.73 and 245.71±3.72 baht/head (P<0.01) and total production costs per weight gain were found to be 38.78±7.90, 37.93±7.92, 38.85±7.92 and 48.79±7.90 baht/head, respectively.

Incidence of grass and weed sensitization in Bangkok, Thailand: a clinical study.

Allergic rhinitis (AR) is a prevalent public health concern globally, significantly impacting quality of life. In Thailand, the prevalence of AR is rising, with grass and weed pollen identified as primary outdoor triggers. This study aimed to (1) assess patterns of pollen sensitization in Thai AR patients and (2) investigate correlations between demographics/clinical data and SPT results. A total of 121 individuals aged ≥18 years with clinically diagnosed AR were recruited. Skin prick testing (SPT) was performed using a panel of commonly encountered tropical grass and weed pollen extracts. SPT wheal sizes and clinical symptom scores were recorded. Correlations between SPT outcomes and symptom scores were analyzed. Among the participants, 104 (85.95%) exhibited positive SPT reactions to at least one pollen type. Nutsedge (76/121), para grass (57/121), and Bermuda grass (48/121) were the most frequently identified allergens. Hurricane grass elicited the strongest reaction, evidenced by the highest average wheal size (6.2 mm). Poly-sensitization was observed in 77 (63.6%) of the SPT-positive individuals, with most cases involving two different pollen extracts (35/77). Notably, AR severity positively correlated with both average wheal size and the number of positive SPT tests. This study highlights nutsedge, para grass, and Bermuda grass as major allergenic pollen sources for Thai AR patients. Including nutsedge, hurricane grass, and careless weed in clinical SPT panels is recommended for improved diagnostic accuracy. Additionally, the positive correlation between AR severity and pollen reaction strength emphasizes the importance of implementing patient education and avoidance strategies.

Enzymatic Activities in the Rhizosphere Soil as Influenced by Filterbeds and Hydrophytes in the Vertically Constructed Wetland

An experiment with different filter beds and macrophytes was carried out to study their phytoremediation capacity on the efficiency of domestic wastewater treatment through constructed wetland (CW) at the University of Agricultural Sciences, Dharwad campus, Karnataka, between November 2017 and March 2018. Twenty treatment combinations involving five types of filter beds (FB-1: gravel, FB-2: gravel-sand-gravel, FB-3: gavel-sand-brick-gravel, FB-4: gravel-sand-charcoal-gravel and FB-5: gravel-sand-(charcoal+brick)-gravel) and four macrophytes (MP-1: Typha latifolia, MP-2: Brachiaria mutica, MP-3: Canna indica and MP-4: Phragmites sp.) were evaluated for treating domestic waste water. After 120 days from the start, across treatment combinations, the plant in each column was uprooted, and the soil adhered to the root was collected in a polyethylene cover. The samples were brought to the laboratory, refrigerated, and then analysed for dehydrogenase, phosphatase, and urease activities, as well as for biofilm formation. Results revealed that the highest biofilm growth was observed on brick (1.18 and 0.05 mg g-1) had the maximum biofilm formation, followed by sand (0.68 and 0.04 mg g-1), charcoal (0.67 and 0.02 mg g-1), and gravel (0.31 and 0.01 mg g-1) at depths ranging from 0 to 5 and 5 to 15 cm, respectively. Since the CW can be implemented with flexibility, it can be used as the primary, secondary or tertiary treatment stage depending on the site and its configuration.

Growth, yield and economics of fodder grasses at Prayagraj, Uttar Pradesh

To study the growth, yield and economics of fodder grasses at Prayagraj in Uttar Pradesh, experiment was established in July 2022 with three treatments and five replications. In this study planted three fodder grasses viz., Pennisetum purpurmum × Pennisetum typhoides (Hybrid napier), Brachiaria mutica (Para grass) and Stylosanthes hamata (Stylo). Results indicated that the maximum height was found in T1: Hybrid Napier 186.60 cm followed by T2: Para grass 132 cm and minimum in T3: Stylo 57 cm. The plant population was maximum found in T3: Stylo 16 followed by T1: Hybrid Napier and T2: Para grass 4 whereas number of tillers or branches per meter square found maximum in T3: Stylo 159.80 followed by T1: Hybrid Napier 158.60 and minimum in T2: Para grass 120. The green fodder yield and dry fodder yield was maximum found in T1: Hybrid Napier 860.16 and 266.65 qha-1 followed by T2: Para grass 438.77 and 122.86 qha-1 and minimum in T3: Stylo 194.65 and 36.98 qha-1 respectively. The maximum net return was found in T1: Hybrid Napier Rs. 75,660 followed by T3: Stylo Rs. 62.505 and minimum in T2: Para grass Rs. 61,605. The maximum B:C ratio was found in T1: Hybrid Napier 2.86 followed by T3: Stylo Rs. 2.61 and minimum in T2: Para grass Rs. 2.29.

Tolerance to drought and flooding events provides a competitive advantage for an invasive over a native plant species

Abstract Changes in precipitation patterns are one of the most pervasive components of climate change. It has been suggested that the increased frequency of extreme drought and flooding events could affect the outcome of competition between native and invasive plants. However, empirical evidence for this prediction remains scarce. We combined controlled experiments and field observations in a freshwater floodplain to assess how drought and flooding events affect growth and biomass production of a native and an invasive plant species, grown in monoculture and mixed culture. We used the native Panicum dichotomiflorum and the invasive Urochloa mutica, which are two common grasses that frequently grow in natural floodplains of the Neotropics. The experimental and observational data showed that biomass production, growth and relative dominance of the native species were higher under more stable water levels. By contrast, the invasive species had higher biomass production, growth and relative dominance under extreme drought and flooding conditions. Thus, extremes in precipitation can alter the relative dominance of the two species in favour of the invader. Our study provides comprehensive evidence that water‐level oscillations may negatively impact the performance of the native species, whereas they did not alter the biomass production and growth of the invasive species. Under stable conditions, faster growth of the native species may hinder successful establishment of the invasive species. However, under extreme drought and flooding events, the invader could be favoured. Although our findings are based on the outcome of the interaction between only one native and one invasive plant species, our results suggest that biotic resistance might be higher under stable water conditions.

Impact of Cropping Systems on Soil Organic Carbon Levels and Enzyme Activity

Monocropping makes agriculture more susceptible to the adverse effects of climate change. Resilient agriculture systems that can buffer crops against various agricultural vulnerabilities is the need of the hour. In this context, a study was conducted to assess the impact of various rice based cropping systems on soil health.
 Aims: To study the effect of different rice based cropping systems on soil enzyme activity and organic carbon.
 Study Design: Randomized block design, Replications – 3.
 Place and Duration of Study: Integrated Farming System Research Station (IFSRS) of Kerala Agricultural University located at Karamana, Thiruvananthapuram, during kharif, rabi and summer seasons of 2019-2020.
 Methodology: The experiment consists of eleven cropping systems (T1 : rice - fallow - fallow, T2 : rice - rice - fallow, T3 : rice - daincha - cowpea, T4 : (rice + daincha) - rice - green gram, T5 : rice - cassava - amaranthus, T6 : rice - cassava - cowpea, T7 : rice - para grass - fodder cowpea, T8 : rice - fodder cowpea - fodder maize, T9 : rice - okra - culinary melon, T10 : rice - okra - yard long bean and T11 : rice - rice – amaranthus).
 Results: Soil organic carbon and enzyme activities were found highest in the cropping system (rice+daincha) – rice – green gram during kharif. During rabi, soil organic carbon and activity of urease and acid phosphatase were highest in rice- daincha- cowpea while dehydrogenase activity was highest in rice- fodder cowpea – fodder maize. During summer, rice- okra – yard long bean, rice- cassava- amaranthus and rice- cassava – cowpea recorded the highest urease, acid phosphatase and dehydrogenase activity, respectively. The organic carbon was highest for the sequence rice – okra –culinary melon. The soil organic carbon and enzyme activities were least in rice- fallow-fallow cropping sequence during all the three cropping seasons.
 Conclusion: Brown manuring, use of leguminous crops and cover crops in the crop sequence instead of leaving the land fallow and addition of organic sources of nutrition increase the soil organic carbon and enzymatic activity of microbes. The increase in soil organic carbon and activity of enzymes in soil owing to crop rotation of rice with arable crops relies on the crop species which are cultivated.

Exploring the potential of bacterial-augmented floating treatment wetlands for the remediation of detergent-contaminated water

Due to industrialization and urbanization, the use of detergents inadvertently led to contamination of aquatic environments, thus posing potential threat to aquatic organisms and human health. One of the main components of detergents is linear alkylbenzene sulfonate (LAS), which can cause toxic effects on living organisms, particularly aquatic life in the environment. In this study, floating treatment wetlands (FTWs) mesocosms were developed and augmented with LAS-degrading bacteria. The plant species, Brachiaria mutica (Para grass), was vegetated to establish FTWs and bacterial consortium (1:1:1:1) of Pseudomonas aeruginosa strain PJRS20, Bacillus sp. BRRH60, Acinetobacter sp. strain CYRH21, and Burkholderia phytofirmans Ps.JN was augmented (free or immobilized) in these mesocosms. Results revealed that the FTWs removed LAS from the contaminated water and their augmentation with bacteria slightly increased LAS removal during course of the experiment. Maximum reduction in LAS concentration (94%), chemical oxygen demand (91%), biochemical oxygen demand (93%), and total organic carbon (91%) was observed in the contaminated water having FTWs augmented with bacterial consortium immobilized on polystyrene sheet. This study highlights that the FTWs supported with immobilized bacteria on polystyrene sheets can provide an eco-friendly and sustainable solution for the remediation of LAS-bearing water, especially for developing countries like Pakistan.

Studi Kecernaan dan Konsumsi Pakan pada Kambing Peranak Etawah Jantan Muda yang diberi Pakan Hijauan Mengandung Tannin Kondensasi

This study aims to determine the effectiveness of using various levels of forage sengon leaves (Albizia falcataria) as a source of condensed tannin on dry matter intake (DMI), organic matter intake (OMI), digestible dry matter intake (DDMI), digestible organic matter intake (DOMI), dry matter digestibility (DMD), and organic matter digestibility (OMD). This study used sixteen young male ettawa cross-breed goats aged 8–12 months with an average body weight range of 19±1.8 kg. The study was arranged in a randomized block design (RBD) with four treatments and four bodyweight groups as replicates. Diet treatments consisted of R0: 60% Brachiaria mutica (BM) + 0% A. falcataria (AF) Leaf + 40% Concentrate (CON), R1: 50% BM + 10% AF Leaf + 40% Concentrate, R2: 40% BM + 20% AF Leaf + 40% CON, and R3: 30% BM + 30% AF Leaf + 40% CON. The results showed that the use of sengon leaves as a source of condensed tannin in diets significantly (P 0.05) reduced DMI, OMI, DDMI, and DOMI but did not significantly (P > 0.05) affect DMD and OMD values. This study concluded that AF leaf as a forage source containing condensed tannin made up as much as 30%, or equivalently, 50%, of the forage in the diet of young male ettawa cross-breed goats without having any effect on diet intake or digestibility.

In vitro CHEMICAL AND FERMENTATIVE CHARACTERISTICS OF TROPICAL PASTURES AT DIFFERENT AGES OF REGROWTH

The low nutritive value of pastures in tropical regions represents a limitation in the productivity of grazing ruminants. The objective was to determine the in vitro chemical and fermentative characteristics of nine tropical grasses (Aruana [Panicum maximun Jacq. cv. Aruana], Bermuda [Cynodon dactylon L.], Estrella de África [Cynodon Cynodon nlemfuensis Vanderyst], Insurgente [Brachiaria brizantha Hochst. Stapf.], Llanero [Andropogon gayanus Kunth], Mombaza [Panicum maximun Jacq. cv. Mombaza], Pará [Brachiaria mutica Stapf], Pangola [Digitaria eriantha Steud.], and Tanzania [Panicum maximun Jacq. cv. Tanzania]) at three regrowth ages (30, 45, and 60 days). The chemical analysis was used to determine the concentrations of dry matter (DM), crude protein (CP), acid detergent fiber (ADF), neutral detergent fiber (NDF), and ash (Ce). The in vitro trial measured partial (24, 48, and 72 h) and cumulative biogas and methane production, fermentation kinetics estimators (A, b, and k), dry matter degradation (DMD) and detergent neutral fiber degradation (NDFD), pH, and ammonia nitrogen (N-NH3). The experimental design was completely randomized at each regrowth age. In conclusion, based on chemical analysis and fermentative characteristics, Pangola and Estrella pastures performed better at each regrowth age.

A Pilot Study to Identify Grass Species That Mediate Pollen Allergy in Thailand

Introduction: Some grass species that are either common or widely spread in Thailand have not been used for pollen allergy diagnosis. In order to improve diagnostic accuracy, the aim of this pilot study was to identify the grass species responsible for pollen allergy in Thailand. Methods: The skin sensitization of pollen extracts from six different grass species, including rice (Oryza sativa), corn (Zea mays), sorghum (Sorghum bicolor), para grass (Urochloa mutica), ruzi grass (Urochloa eminii), and green panic grass (Megathyrsus maximus), was evaluated by skin prick test (SPT). Serum’s IgE specific to each pollen extract was analyzed by Western blot (WB). The ImmunoCAPTM test for Johnson grass was also evaluated. Results: Of the thirty-six volunteers who participated in this study, eighteen tested positive for at least one of the diagnostic tests, namely SPT, WB analysis, or ImmunoCAPTM. Notably, skin reactivity to para grass, corn, sorghum, and rice was more commonly observed compared to ruzi grass and green panic grass. However, in the WB analysis, individuals with pollen-specific IgE were more frequently detected in sorghum, green panic grass, corn, rice, and ruzi grass than para grass. Conclusion: In this pilot investigation, our findings indicate that the pollen extracts of rice, corn, sorghum, and para grass are associated with pollen allergy in Thailand. These results contribute to the current knowledge on the identification of grass species that are associated with pollen allergy in Thailand and Southeast Asia.

Soil macrofauna diversity and weed dynamics in response to different methods of weed control in smallholder rubber farming

Abstract. Chaniago I, Yulistriani, Umami IM, Bukhari ZZ. 2023. Soil macrofauna diversity and weed dynamics in response to different methods of weed control in smallholder rubber farming. Biodiversitas 24: 3106-3113. Weeds interference in rubber farmland may reduce latex yield. Farmers apply herbicides to control weeds. However, the herbicide not only controls weed but also affects the presence of soil macrofauna. A study has been conducted to determine the effect of different method of weed control on soil macrofauna and weeds at smallholder rubber farming at Pulau Punjung, Dharmasraya District, West Sumatra, Indonesia. The experiment was conducted in a completely randomized block design with 6 treatments and 4 pseudo-replications. Treatment was mechanical control, without weed control, and 4 doses of herbicide glyphosate+metsulfuron-methyl (1.5 L ha-1 + 15 g ha-1, 2 L ha-1 + 15 g ha-1, 2.5 L ha-1 + 15 g ha-1, 3 L ha-1 + 15 g ha-1) with 400 L ha-1 of volume. Pitfall traps were used to collect the soil macrofauna. Data were analysis with ANOVA for weed and soil macrofauna in response to different methods of weed control. Results demonstrated that the application of glyphosate+metsulfuron-methyl herbicide significantly suppressed weeds higher than that of other methods of weed control (p<0.05). Para grass (Brachiaria mutica (Forssk.) Stapf) was the most dominant weed but has been affected most by 3 L ha-1 + 15 g ha-1 herbicide mixture. Soil macrofauna of the order Hemiptera was affected most by the application of herbicide with a total reduction of 71.58% of number of individuals at 12 weeks after application of herbicide. In general, different weed control methods resulted in varied numbers of orders and individuals of the soil macrofauna.

Effect of Fish Oil and Linseed Oil on Intake, Milk Yield and Milk Fatty Acid Profile in Goats.

This study aimed to evaluate the effect of incorporating linseed oil and fish oil in the diet on intake, ruminal fermentation, milk yield, and milk fatty acid profiles in dairy goats. Four crossbred Saanen lactating goats in mid-lactation and milking 1.30 ± 0.28 g/day were used in a 4 × 4 Latin square design. The basal diet contained concentrate and Para grass (C:F 40:60). Treatments included a basal diet without oil supplementation (Ctrl) or with 2.5% linseed oil (LO2.5), 2.5% linseed oil and fish oil (3:2, w/w, LFO2.5), and 4.16% linseed oil and fish oil (3:2, w/w, LFO4.16). Diets had no effect on intake, milk yield, milk composition, or ruminal fermentation (p > 0.05). Compared with Ctrl, lower (p < 0.05) proportions of C10:0-C14:0 in milk fat were observed with LFO4.16. Compared with the Ctrl and linseed oil added alone, feeding LFO4.16 led to a greater (p < 0.01) concentration of C18:1 t11. Compared with both the Ctrl and LO2.5 diets, milk c9,t11 CLA was 4.53 and 2.94 times greater with the LFO4.16 diet. Compared with Ctrl and LO2.5 diets (0.06% and 0.08%), goats fed LFO2.5, and LFO4.16 had greater (p < 0.001) concentrations of C22:6n-3 (0.63% and 0.87%). Overall, the combined data suggested that including 4.16% linseed oil and fish oil in the diet of dairy goats was effective in improving the concentrations of health-promoting fatty acids in milk without affecting milk production.

Evaluation of grass pollen quality control for allergy test kit production using pollen morphological characters

Grass pollen is one of the leading causes of allergies in throughout the world. The symptom level depends on the personal immunity. Pollen allergy test kits are suitable for detecting allergic symptoms, which they are produced from pure pollen of specific species. The production of pollen allergy testing kits requires accurate botanical standards and identification for the process of quality control of raw materials basing on Thai Food and drug ministration (Thai FDA). To produce allergy test kit, we focused on the pollen of these 6 common grass species and also use pollen morphological characters for detection and identification. Therefore, this study focused on the method for qualification control and standardization for prototype product of grass pollen allergy testing kit. Six common grass species as Megathyrsus maximus (Jacq.) B.K.Simon &amp; S.W.L. Jacobs, Oryza sativa L., Sorghum bicolor (L.) Moench, Urochloa eminii (Mez) Davidse, Urochloa mutica (Forssk.) T.Q.Nguyen and Zea mays L.). The criteria were: 1) common in Thailand and Southeast Asia; 2) high density in the atmosphere; and 3) severity of the allergic reaction. The modified acetolysis were applied for samples preparation. The pollen morphological (polar and equatorial axes, exine, porus, annulus and ornamentation) were observed and measurement under light and scanning electron microscope. The character matrix was analyzed based on a regression tree and Tukey’s HSD test to assess support for the different pollen morphological characters. The distinguished character is ornamentation. The results were successfully described the pollen species and showed that the pollen morphologies were useful for contaminated detection. In addition, this method was suitable applied for qualification control of the raw materials for grass pollen allergy testing kits production.

Effects of using Water Hyacinth (Eichhornia crassipes L.) in the Diet of Swamp Buffaloes on Nutrient Digestibility, Rumen Environment, Purine Derivatives, and Nitrogen Retention

The present experiment aims to evaluate the effects of incremental levels of water hyacinth (WH) in Para grass (Brachiaria mutica) based on the diet on nutrient intake, digestibility, and nitrogen retention of growing swamp buffaloes. Four male buffaloes of 305 ± 8.40 kg were allocated in a 4x4 Latin square design. The treatments were 25, 50, 75, and 100% WH (DM basis), replacing the Para grass (PG) corresponding to WH25, WH50, WH75, and WH100 treatment. Urea-molasses cake was used to supply the dietary crude protein intake so that this was 210 g/100 kg live weight per day for all the treatments (DM basis). The results showed that although the dry matter (DM), neutral detergent fiber (NDF), and metabolizable energy (ME) intake were not significantly different (P&gt;0.05) among the treatments, these were numerically higher for the WH50 treatment. Especially in the daily ME intake (MJ/k g LW) was 39.1, 43.4, 41.7, and 39.9 for the WH25, WH50, WH75, and WH100 treatments, respectively. With 50% WH replacing PG in the diet, it gave potential for better nutrient digestibility, nitrogen retention, and daily weight gain in the present study. In conclusion, WH could be used to replace PG in buffalo diet up to 100%. At a level of 50% replacement of WH to PG in the buffalo, the diet was optimum based on the utilization of nutrients and energy of WH, lower feed cost, and environmental improvement.

Performance of Perennial Grass Species under Supplemental Irrigation

The study was conducted at Agricultural Research Centre with the objective of identifying the best-performing perennial grass species under supplemental irrigation and advising the promising cultivated fodder grass for smallholder livestock producers. Randomized Complete Block Design of six perennial grass species with three replications was employed. The six perennial grass species used were T1 (Brachiaria mutica_18659), T2 (Cynodon aethiopicus), T3 (Pennisetum sphacelatum), T4 (Brachiaria decumbense), T5 (Chloris gayana cv. Masaba), and T6 (Brachiaria mutica_6964). The result revealed that agronomic performance of cover, vigor, and height was varied (P&lt;0.001) among perennial grass species. Furthermore, effects of cover (P&lt;0.05), vigor (P&lt;0.05) and height (P&lt;0.001), and dry matter contents (P&lt;0.05) of perennial species over years were found. The dry matter yields and contents of P. sphacelatum (22.85 ton ha-1 and 41.7%) followed by C. aethiopicus (12.53 ton ha-1 and 40.6%) of perennial grass species performed better (P&lt;0.001) than others. The highest (P&lt;0.001) dry matter yield (20.3 ton ha-1) was obtained during the second year of harvest than the first (12.65 ton ha-1) and third (11.36 ton ha-1) year harvests. Similarly, the highest (P&lt;0.001) dry matter percent of the perennial grass species was also found during the second year harvest (50.1%) than the first (25.8%) and third (40.0%) year harvests. The lowest dry matter yield was recorded by C. gayana at the first year (8.5 ton ha-1) and third year (8 ton ha-1) harvests. Likewise, B. mutica_18659 provided the lowest dry matter yield (12.9 tons ha-1) during the second harvest period. Hence, smallholder farmers can preferably be advised to use P. sphacelatum and C. aethiopicus perennial grass fodder crops resources under supplemental irrigation.