Paper Chromatography Of Sugars Research Articles

First report of congenital or infantile cataract in deranged proteoglycan metabolism with released xylose

AIMTo investigate the chemical pathology in the blood and lens, in cases of congenital or infantile cataract in children excreting predominantly non-reducing carbohydrates in urine.METHODSUrine samples from children with congenital...

Some technical improvements in the paper chromatography of sugars : A method of sample desalting and a sensitive staining reagent

Some technical improvements in the paper chromatography of sugars : A method of sample desalting and a sensitive staining reagent

A Study of Trailing in the Paper Chromatography of Sugars.

A Study of Trailing in the Paper Chromatography of Sugars.

A new detection method in paper chromatography of sugars

A new detection method in paper chromatography of sugars

Detection and Paper Chromatography of Sugars and Sugar Phosphates in Picric Acid System

Detection and Paper Chromatography of Sugars and Sugar Phosphates in Picric Acid System

A New Solvent for Quantitative Paper Chromatography of Sugars

MANY mobile phases are found in the paper chromatography of sugars; but the separation of sucrose, fructose and glucose is somewhat difficult, because their R F values are very near each other1. The separation is enough for identification purposes, but it is not good enough for elution or photometric readings, because the sugars are still partly mixed. The solvent suggested below divides the sugars quite well with the following R F values (at 20° C.): sucrose, 0.17; glucose, 0.26; mannose, 0.30; arabinose, 0.32; fructose, 0.32; xylose, 0.33; ribose, 0.41.

Qualitative Paper Chromatography of Sugars in Plants

Qualitative Paper Chromatography of Sugars in Plants

Use of unneutralized hydrolysates in paper chromatography of sugars.

IN preparing hydrolysates of polysaccharides for paper chromatography, it has always been considered necessary to neutralize the sulphuric acid with barium carbonate before applying the solution to the paper, as the acid causes disturbing stains. Indeed, when using an ammoniacal silver spray, white stains appear at the place where the spots of glucose and galactose should be; but the flow of the sugars does not seem to be disturbed.

Use of ion-exchange resins in paper chromatography of sugars.

THE recent communication by Phillips and Pollard1 suggests that results obtained here may be of interest. In the paper chromatography of dilute and very impure sugar solutions, such as non-diabetic urines or certain protein hydrolysates, it is common practice to use beforehand a ‘mixed bed’ ion-exchange resin to remove interfering anions and cations2. For example, ‘Bio-Deminrolit E’ a mixture of a weakly basic resin containing amine groups and a sulphonic acid type resin, is shaken with the solution for 20 min. and, after centrifuging, the clear supernatant contains all the sugars (except amino-sugars and uronic acids) uncontaminated by ionizable substances3. When ‘Bio-Deminrolit E’ was replaced by ‘Bio-Deminrolit’ or ‘Bio-Deminrolit F’, which are mixtures of sulphonic acid type resins with strongly basic resins of the quaternary ammonium hydroxide type, it was found that all fructose, glucose, galactose, mannose and fucose were removed from the dilute solutions employed.