Paniculata Extracts Research Articles

High-Throughput Screening System Evaluation of Andrographis paniculata (Burm.f.) Extracts and Their Fractions against Mosquito Vectors.

Infectious diseases that cause illness and/or death in humans can be contracted from mosquito bites. A viable and alternate method of personal protection that can lower the danger of human exposure to mosquito-borne diseases is the use of plant-based repellents. Using a high-throughput screening system, the current work examined the toxicity, contact irritancy, and spatial repellency of Andrographis paniculata crude extract and its fractions against Aedes aegypti, Anopheles minimus, and An. dirus. Five fractions (i.e., F1, F2, F3, F4, and F5) were separated from the crude extract by column and thin layer chromatography and analyzed using high-performance liquid chromatography and mass spectrometry. The major active compounds identified from F3 and F5 were 4-deoxy-11,12-didehydroandrographolide and andrographolide. Three concentrations (1.0, 2.5, and 5.0%) for each of the crude extracts and the five fractions were individually impregnated on nylon netting strips and evaluated against the three mosquito species. Results showed that the highest contact irritancy was elicited by the crude extract at 5% concentration against Ae. aegypti (43.70% escaped). Results of the spatial activity index (SAI) showed that fractions F3 and F5 at 2.5% demonstrated the strongest repellency against Ae. aegypti (SAI = 0.84) and An. minimus (SAI = 0.83), respectively. Both the crude extract and its components did not cause any knockdown or mortality. These findings suggest that fractionation of A. paniculata extracts is valuable in assessing their spatial repellent efficacy against mosquitoes. Fractions F3 and F5 hold promise as natural mosquito repellents and could contribute to developing effective mosquito control strategies.

Antifungal properties of Andrographis paniculata extracts as potential biofungicides: a review

Andrographis paniculata, commonly known as the king of bitters, has been studied for its potential as a natural fungicide due to its several bioactive compounds, including andrographolides. The primary objective of this study was to demonstrate the possible efficacy of A. paniculata against a wide range of fungal diseases in terms of the properties of A. paniculata extracts that could potentially be used as a bio-fungicide. The presence of alkaloids, flavonoids, phenols, terpene derivatives compounds, and other phytochemical constituents makes the A. paniculata extract more precise in its mode of action against the variety of phytopathogenic fungi. Various physiological and biochemical changes in crops result in unintended physiological issues, degradation, and subsequent financial losses during storage. Synthetic fungicides have been applied to control plant diseases caused by phytopathogenic fungi. However, their application has drawn criticism because of adverse effects. Therefore, a possible solution would be to apply botanical fungicides, which have been proven to be generally non-phytotoxic, systemic, and rapidly disposable in nature. Botanical fungicides are endowed with benefits such as resistance inhibition, being ecofriendly, effective, elective, and more affordable than synthetic fungicides. Therefore, the medicinal herb, A. paniculata has been proven to be effective in controlling pathogens of agricultural crops and increased their shelf-life when used as a substitute for synthetic chemicals. Due to their antimicrobial capabilities against fungal, bacterial, and viral infections, various medicinal herbs have been investigated and employed for crude extraction globally. Andrographis paniculata extracts have broad-spectrum antifungal activity, indicating potential efficacy against a diverse variety of fungal infections.

Exploring the Enhanced Bioactivity of Selenium Nanoparticles Synthesized with Andrographis paniculata Extracts: A Cross-Cultural Comparison Between Thailand and India

Exploring the Enhanced Bioactivity of Selenium Nanoparticles Synthesized with Andrographis paniculata Extracts: A Cross-Cultural Comparison Between Thailand and India

Effectiveness of Phyllanthus niruri and Andrographis paniculata Extracts on Egg Quality in Laying Hens with Avian Pathogenic Escherichia coli

Providing sufficient and high-quality eggs plays an essential role in accommodating food safety and food security for consumers. The purpose of this study was to determine the effect of Phyllanthus niruri and Andrographis paniculata extracts on egg yolk index and haugh units (HU) value in laying hens with Avian Pathogenic Escherichia coli. This type of study was experimental using a complete randomized design with 100 experimental units of five treatments (P-) placebo, (P+) infected with Avian Pathogenic E. coli without P. niruri and A. paniculata extract, then groups of chickens infected with Avian Pathogenic E. coli and administration of (P1) 10% of P. niruri and 30% of A. paniculata, (P2) 20% of P. niruri and 20% of A. paniculata, (P3) 30% of P. niruri and 10% of A. paniculata then egg yolk index and HU value were evaluated. The data obtained were analyzed using ANOVA followed by Duncan’s test. The results showed a significant difference between the treatment groups based on the egg yolk index and the HU value. It can be concluded that P2 can be recommended for laying hens with avian pathogenic E. coli.

Inhibitory efficiency of Andrographis paniculata extract on viral multiplication and nitric oxide production.

Andrographis paniculata (Burm. F.) Nees is a medicinal plant previously reported with broad-spectrum antivirals but the mode of inhibition remains elusive. The objective of this study was to identify the most active fraction from A. paniculata ethanol extract (APE, APE-2A, APE-2B and APE-2C) and dry powder extract (APSP) against influenza A (H3N2), representing RNA viruses, and herpes simplex virus-1 (HSV-1), representing DNA viruses. The results showed that the fractions APSP, APE, APE-2B, and APE-2C directly neutralized the HSV-1 and influenza A (H3N2) when incubated at room temperature for 60min before infecting the cells. The results also showed that the additional APE-2A fraction also directly neutralized the influenza A (H3N2), but not the HSV-1. The APE, APE-2B and APE-2C inhibited the HSV-1 by more than 0.5 log when the fractions were introduced after infection. Similarly, the APSP and APE inhibited the influenza A (H3N2) more than 0.5 log after infection. Only 50μg/mL APE-2C inhibited the viruses greater than 0.5 log. In addition, A. paniculata extracts were also evaluated for their interfering capacities against nitric oxide (NO) production in LPS-activated RAW 264.7 macrophages. As well, APE-2C potently inhibited NO production at the IC50 of 6.08μg/mL. HPLC and LC-MS analysis indicated that the most actively antiviral fractions did not contain any andrographolide derivatives, whereas the andrographolide-rich fractions showed moderate activity.

Comparative Study of Optical and Chemical Properties of ZnO-Ag Composites Synthesized from Mimosa Pudica and Andrographis Paniculata Extracts

In this research, a comparison was made between the optical and chemical properties of ZnO-Ag composites with different ZnO and Ag ratios synthesized using extracts obtained from mimosa and Andrographis paniculata plants. The optical properties of the synthesized ZnO-Ag composites were analyzed, focusing on parameters such as absorption and bandgap energy. Moreover, the bandgap, which indicates the energy difference between the valence and conduction bands, was calculated to assess the compounds' electronic behavior. Furthermore, the chemical properties of the ZnO-Ag composites were investigated. This involved analyzing the chemical bond, crystal structure, and crystalline size using X-ray diffraction (XRD) and Fourier Transform Infrared Spectrometer (FT-IR). The experimental findings demonstrated that the crystal sizes of ZnO and Ag in ZnO-Ag composites synthesized using Mimosa pudica extract were considerably smaller compared to those synthesized using Andrographis paniculata extract. Consequently, the energy gap of the ZnO-Ag compounds synthesized with mimosa extract was higher in comparison to those synthesized with Andrographis paniculata extract. By comparing the optical and chemical properties of the ZnO-Ag composites synthesized from mimosa and Andrographis paniculata extracts, valuable insights can be gained regarding the influence of these plant extracts on the resulting composites.

Botanicals as Quorum Quenching Molecules and Their Effect in Plant Growth Promoting Traits in Pantoea, Paenibacillus, and Rhizobium

Quorum sensing is a cell-to-cell communication mechanism that was reported in Rhizobium mayense S11R1, Pantoea dispersa YBB19B, and Paenibacillus illionoisensis YBB20 by the presence of N-acyl homoserine lactones. This study explored possible mechanisms for expressing plant growth-promoting traits meditated through the quorum-sensing molecules. To prove the above hypothesis, a quorum quenching approach was carried out to study the regulation of plant growth-promoting traits of three isolates using botanicals like Curcuma longa (Turmeric) and Andrographis paniculata (Nilavembu) extracts (500, 1000, and 1500 ppm). At 1500 ppm, indole acetic acid production was completely inhibited in all three isolates. Extracellular Polymeric Substances (EPS) production was significantly reduced in Pantoea dispersa YBB19B (24 µg/mL) when treated with nilavembu extract. Biofilm-forming ability was weakened to a greater extent in Pantoea dispersa YBB19B and Paenibacillus illionoisensis YBB20 when treated with nilavembu extract. Hence, it is concluded that plant growth promotion traits in the selected bacteria are mediated through quorum sensing.

Assessment of the Phytochemical Profile, Antioxidant Capacity, and Hepatoprotective Effect of Andrographis paniculata against CCl4-Induced Liver Dysfunction in Wistar Albino Rats.

Recent studies have highlighted the necessity to thoroughly evaluate medicinal plants due to their therapeutic potential. The current study delves into the phytochemical profile, antioxidant capacity, and hepatoprotective effect of Andrographis paniculata. The investigation specifically targets its effectiveness in mitigating liver dysfunction induced by carbon tetrachloride (CCl4) in Wistar albino rats, aiming to uncover its promising role as a natural remedy for liver-related ailments. A. paniculata leaf extract was screened for phytoconstituents and antioxidant and hepatoprotective effects in Wistar albino rats against CCl4-induced liver dysfunction. Phytochemical analysis revealed the presence of flavonoids, alkaloids, and phenolic compounds in all extracts. The phenolic concentration ranged from 10.23 to 19.52 mg gallic acid per gram of the sample, while the highest flavonoid concentration was found in the ethanol fraction (8.27 mg rutin equivalents per gram). The antioxidant activity varied from 10.23 to 62.23. GC-MS analysis identified several phytochemicals including octadecanoic acid, stigmasterol, phenanthrenecarboxylic acid, and others. Effects of the ethanol extract of A. paniculata were evaluated in four groups of animals. Biochemical estimations of serum glutamine oxaloacetate transaminase, serum glutamine pyruvate transaminase, and serum bilirubin were significantly higher (p < 0.05) in the CCl4-treated group. Treatment with 300 mg/kg b.w. of the ethanol extract of A. paniculata significantly (p < 0.05) decreased these serum enzymes. Lipid peroxidation levels in carbon tetrachloride-treated animals showed a substantial (p < 0.05) rise when compared to untreated animals, while the lipid peroxidation levels were considerably (p < 0.05) reduced after treatment with ethanol extract at 300 mg/kg b.w. Liver biochemical catalase activities were significantly reduced in the carbon tetrachloride-treated animals. The results of this study conclusively demonstrate that A. paniculata extracts are a rich source of phytochemicals and possess significant antioxidant, free radical scavenging, and hepatoprotective properties.

GABA content and an antioxidant profile positively correlated with the anticonvulsive activity of Microcos paniculata in acute seizure mice

This study evaluated the effects of different parts of M. paniculata (MP) extracts on convulsions and antioxidant activities in mice. Six polyphenolic compounds were identified, where epicatechin and quercetin have been identified in the highest amounts (23.01 and 32.23 mg/100 g of dry MP extract, respectively) in MP leaf and stem extracts, using Ultra Performance Liquid Chromatography. 7-day oral administration of MP at doses of 100, 200, and 400 mg/kg body weight (BW) significantly reduced convulsions and reduced mortality rates compared with seizure inducer groups. Antioxidant potentials were measured by superoxide dismutase (SOD), catalase (CAT), thiobarbituric acid reactive substances (TBARS), and reduced glutathione (GSH) content in whole-brain homogenates. Gamma-aminobutyric acid (GABA) levels significantly increased in leaves and stem-treated groups, suggesting that MP leaves and stems have potent antioxidant properties that can attenuate convulsions by modulating the GABAergic system and antioxidant activities.

Comparative Evaluation of Novel Herbal Mouthwash with 12.5% Xylitol Mouthwash and 0.7 M Saline Mouthwash on Plaque Control: An Interventional Study

Background and Aim: Among the wide array of herbal plants possessing medicinal values, Andrographis paniculata (Nilavembu) stands out as a time-tested premiere medicinal herb. A. paniculata (Nilavembu) extracts exhibited varying degrees of inhibitory activity against the growth of all the microorganisms. Hence, this research was made to assess and compare the effect of Nilavembu extract (A. paniculata) with 12.5% xylitol mouthwash and control group (0.7 M saline mouthwash) on dental plaque and gingival inflammation. Materials and Methods: A double-blinded parallel-arm randomized clinical trial was done among 40 participants aged 20–30 years. Participants were randomly allotted into four groups: Group 1 - Nilavembu extract with 0.7 M saline wash; Group 2 - Nilavembu extract with 12.5% xylitol mouthwash; Group 3-0.7 M saline mouthwash; and Group 4-12.5% xylitol mouthwash. The plaque and gingival status were assessed using oral hygiene simplified index and Loe and Silness index at baseline and end of the study (7th day). Statistical analysis was carried out to compare the effect of all four treatments groups using the Kruskal–Wallis test, Wilcoxon sign rank test, and post hoc test; P ≤ 0.05 was considered statistically significant. Results: Among four groups, Group 2 showed a greater mean reduction followed by Group 1 in oral hygiene index score and gingival score. There was a statistically significant difference between the scores of the four groups. Conclusion: It can be concluded that A. paniculata (Nilavembu extract) could serve as a potential herbal alternative in the therapeutic management of dental plaque and gingivitis.

A Brief Review on Murraya paniculata (Orange Jasmine): pharmacognosy, phytochemistry and ethanomedicinal uses.

Murraya paniculata (family-Rutaceae), popularly known as orange jasmine, is the most important evergreen plant. The Rutaceae family is economically significant due to its diverse edible fruits and essential oils. Murraya paniculata extracts (MPE) of leaf have been shown to include phenolic compounds, highly oxygenated flavonoids, flavanones, sesquiterpenoids, polymethoxy glycosides, and coumarins. Cyclocitral, methyl salicylate, trans-nerolidol, cubenol, isogermacrene, -cadinol, and cubeb-11-ene are all abundant in MPE. The usages of various parts of this plant, such as bark, leaves and flower, as a remedy for a variety of ailments as widely recorded in the traditional literature. The plant has anti-diabetic, anti-obesity, antibacterial, anti-implantation, anti-oxidative, cytotoxic, anti-diarrheal, antidepressant and anti-anxiety properties and many others. The goal of the review is to reignite interest in this potential plant, encouraging researchers to continue their research in order to uncover novel therapeutic compounds for the treatment and management of a range of infections. The current review provided a comprehensive overview of this traditional unique plant. The review paves a way for exploring its active chemical elements with substantial pharmacological values further for potential benefits of mankind.

Cytotoxic action of the leaves of Uvaria chamae P.Beauv. and Dicliptera paniculata (Forssk.) I.Darbysh. from Nigeria mediated through intrinsic apoptotic pathway induction in four cancer cell lines

BackgroundOver the years, the morbidity and mortality due to cancer have continued to increase worldwide. Plants are well utilized in Nigeria ethno-medicine for cancer treatment. However, scientific evidence, particularly at the molecular level, has been lacking, and biodiversity loss is a threat. Aim of the studyThe cytotoxic activity of the leaves of six plants used in Nigeria ethno-medicine was investigated and the molecular pathway of cytotoxic action of two active extracts on four cancer cell lines (A549, RD, MCF-7, and HeLa) was also assessed. Materials and methodsCytotoxic activities of the plant extracts were assessed on breast (MCF-7), lung (A549), cervical (HeLa), adenocarcinoma and rhabdomyosarcoma (RD) cells using MTT cell viability assay in a time-dependent manner. The sandwich ELISA method was used to assess the protein expressions (Bcl-2, BAX, and executioner caspase 3) involved in the apoptotic pathway in cancer cells after treatment with cytotoxic extracts. UHPLC-MS/MS approach was used to analyze the possible bioactive phytochemicals of the cytotoxic plant extract. ResultsUvaria chamae and Dicliptera paniculata extracts displayed good cytotoxicity across all cell lines in a time- and concentration-dependent manner (CC50 <30 µg/mL). Increased pro-apoptotic BAX and lowered expression of anti-apoptotic Bcl-2 show the cytotoxic action of the two extracts followed the intrinsic apoptotic pathway compared to untreated cells. The increased expression of executioner caspase 3 indicated that the intrinsic apoptotic pathway was caspase-dependent. ConclusionThe anticancer potential of U. chamae and D. paniculata in this study validates the use of these plants in Nigerian ethno-medicine, highlighting apoptosis as a molecular pathway of their cytotoxicity.

Effect of andrographolide and deep eutectic solvent extracts of Andrographis paniculata on human coronavirus organ culture 43 (HCoV-OC43)

BackgroundAndrographis paniculata (Burm. f.) Nees has demonstrated potential for treating infections caused by coronaviruses. However, no antiviral activity of andrographolide or A. paniculata extracts against human coronavirus organ culture 43 (HCoV-OC43) has been reported. PurposeThis study aimed to evaluate the anti-HCoV-OC43 effect of andrographolide and A. paniculata as well as the correlation between andrographolide concentration and the anti-HCoV-OC43 activity of A. paniculata extracts. MethodsThis study evaluated and compared the in vitro anti-HCoV-OC43 activities of various A. paniculata extracts and andrographolide. To obtain A. paniculata extracts with different concentrations of andrographolide and its components, methanol and deep eutectic solvents (DES) were used to extract the aerial parts of A. paniculata. Andrographolide content was determined using UV-HPLC, and antiviral activity was assessed in HCT-8 colon cells. ResultsThe methanol and five acidic DES (containing malic acid or citric acid) extracts of A. paniculata exerted anti-HCoV-OC43 activity. Antiviral activity had a moderately strong positive linear relationship (r = 0.7938) with andrographolide content. Although the methanol extract contained the highest andrographolide content (2.34 mg/ml), its anti-HCoV-OC43 activity was lower than that of the DES extracts containing lower andrographolide concentrations (0.92–1.46 mg/ml). ConclusionMethanol and the five acidic DES extracts of A. paniculata exhibited anti-HCoV-OC43 activity. However, the in vitro antiviral activity of A. paniculata extracts did not have a very strong positive linear relationship (r < 0.8) with andrographolide concentration in the extract. As a result, when comparing A. paniculata extracts, the anti-HCoV-OC43 test could provide a different result from the andrographolide concentration determination.

Quantification and Standardization of Andrographolide in Andrographis Paniculata Samples by Validated RP-HPLC and HPTLC Methods.

Andrographis paniculata (family Acanthaceae) is known as Kalmegh, one of the traditionally used important medicinal plant contains several biologically active phytochemical including andrographolide. A. paniculata is broadly used by healthcare practitioners in India and also used in different traditional medicinal system. In this study, the leaves of A. paniculata were collected from West Medinipur, East Medinipur, South 24 Parganas, Purulia and Hooghly district of West Bengal, India. This study aiming towards validation and development of a simple, precise and reproducible reverse-phase high-performance liquid chromatography (RP-HPLC) and high-performance thin layer chromatography (HPTLC) methods for quantification of andrographolide in A. paniculata extracts. The validated RP-HPLC and HPTLC study confirmed that different concentrations of andrographolide content present in the plant samples, which are collected from above different districts of West Bengal, India. The amounts of andrographolide were found to be 2.71% (w/w), 3.19% (w/w), 1.83% (w/w), 1.73% (w/w) and 2.94% (w/w) in RP-HPLC study and 2.13% (w/w), 2.51% (w/w), 1.01% (w/w), 1.25% (w/w) and 2.15% (w/w) in HPTLC study. This precise, reproducible, accurate and specific method can be used for the quantification of andrographolide in kalmegh, as per the International Council for Harmonization of Technical Requirements for Pharmaceuticals for Human Use (ICH) guidelines recommendations.

Diversity inPhytochemical composition and Medicinal value ofMurraya paniculata.

Murraya paniculata is herbal medicinal plant which is traditionally being used for management of cardiovascular, intestinal and respiratory (air way) disorders. The goal of this review is to analyze and report the biological activities of active phytochemicalsfrom Murraya paniculata . The data was searched using different search engines and specific keywords including M. paniculata , herbal medicine, phytochemicals, extract, essential oil, bioactivities. M. paniculata has been found to have wide range of pharmacological activities, including antianxiety, antioxidant, antidepressant, antibacterial, analgesic and anti-diabetic properties. A diverse range of phytochemicals, including phenols, coumarins, terpenoids, flavonoids, and alkaloids have been isolated fromthe plant and tested for a variety of biological activities. This review will provide more information and stimulate additional research to develop more effective and cost-efficient alternative medicine from this plant.

Potency of Origanum vulgare and Andrographis paniculata extracts on growth performance in poultry

The objective of this study was to investigate the effect of phytobiotics combination of Origanum vulgare and Andrographis paniculata water extracts (FOA) mixed into the feed of broiler and specific-pathogen-free chickens as an alternative to Antibiotics Growth Promoter (AGP). Performance, intestinal bacteria characteristic, and oocysts of Eimeria spp. in feces were measured and compared with the AGP-added group. The first experiment in broiler chickens compared FOA, Zinc Bacitracin (ZB, as an AGP group), and negative control. On day 28, FOA group and ZB group showed significantly higher body weight than the control group (P < 0.05). The FCR of ZB group was better than FOA group. However, FOA group displayed better microbiota profile than ZB group and negative control, with more Lactobacillus spp. and Bacillus spp., and less Escherichia coli and Salmonella spp. isolated from intestines. The second experiment in specific-pathogen-free chickens showed the anticoccidial effect of FOA addition to reduce the number of oocysts per gram (OPG) from live coccidia vaccine. FOA group and Amprolium group showed OPG reduction (82.53% and 92.02%, respectively) after 7 days of treatment. In conclusion, the combination of Origanum vulgare and Andrographis paniculata extract can function as an AGP replacement in feed.

Andrographis paniculata Dosage Forms and Advances in Nanoparticulate Delivery Systems: An Overview.

Andrographis paniculata is a well-known Asian medicinal plant with a major phytoconstituent of diterpene lactones, such as andrographolide, 14-deoxyandrographolide, and neoandrographolide. A World Health Organization (WHO) monograph on selected medicinal plants showed that A. paniculata extracts and its major diterpene lactones have promising anti-inflammatory, antidiabetic, antimalarial, anticancer, antifungal, antibacterial, antioxidant, and hypoglycemic activities. However, these active phytochemicals have poor water solubility and bioavailability when delivered in a conventional dosage form. These biological barriers can be mitigated if the extract or isolated compound are delivered as nanoparticles. This review discusses existing studies and marketed products of A. paniculata in solid, liquid, semi-solid, and gaseous dosage forms, either as an extract or isolated pure compounds, as well as their deficits in reaching maximum bioavailability. The pharmaceutics and pharmacological activity of A. paniculata as a nano-delivery system are also discussed.

Bio-insecticide’s Extract of Scented Root (Polygala paniculata) in Controlling the Mosquito Aedes aegypti (L.)

Controlling Aedes aegypti mosquitoes with chemical insecticides causes resistance on humans, environmental residues, and contaminates food and water. Polygala paniculata is a potential alternative to insecticides in controlling Ae. aegypti. This study aimed to determine the effect of the Insecticide Score of P. paniculata extracts on the mortality of Ae. aegypti mosquitoes based on KT50. A total of 20 mosquitoes for each concentration were used to examine the effectiveness of 10%, 15%, 20%, and 25% concentration of P. paniculata extracts compared to a positive and a negative control with four replications. The results showed that P. paniculata extracts had an effect on Aeaegypti mosquito mortality. There were significant differences in mortality rate between concentrations (Kruskal-Wallis test, p = 0.001). Spearman Correlation test gave a p-value of 0.008 < 0.05. Similarly, there was a weak but significant correlation between plant extract concentration and mortality (Spearman correlation: r = +0.312, p = 0.008). The 10% and 15% concentrations had KT50with Insecticide Scores of 1 and 2, respectively, implying that both had no knockdown effect. A 20% concentration had a KT50 with an Insecticide Score of 3, indicating a weak knockdown effect, whereas a 25% concentration had KT50with an Insecticide Score of 5, signifying a quick knockdown effect. These results show that a 25% concentration has a quick knockdown time on Ae. aegypti mosquitoes. Therefore, extract P. paniculata extract at a concentration of 25% has a potential for use as a bio-insecticide in controlling Ae. aegypti mosquitoes.

Transcriptomic Profile Analysis of Streptococcus mutans Response to Acmella paniculata Flower Extracts.

Background Acmella paniculata has been used as a traditional medicine to treat oral health diseases such as dental caries and periodontitis. Streptococcus mutans is a common bacterium that initiates dental caries at an early stage. Aim The aim of this study was to determine the mode of action of A. paniculata (extracts) against S. mutans growth. Methods Time-kill assay has been done to investigate the rate of kill and effectiveness of Acmella paniculata (AP) extracts against S. mutans growth. Phytochemical analysis was done to identify major compounds in AP extracts using gas chromatography mass spectrometry (GCMS). Scanning and transmission electron microscopy (SEM and TEM) have been done to observe the morphological changes of treated bacteria. Transcriptomic profile analysis has been done using Next Gene Sequencing. Results AP flower n-hexane (APFH) and AP flower dichloromethane (APFD) extracts acted as bactericidal agents after killing >3 log10 cfu/mL of S. mutans after 24 hours. Oleic and hexadecenoic acids were found to be the major compounds in APFD and APFH extracts, respectively. Photomicrographs from SEM and TEM of treated S. mutans show that the bacterial cell wall has been lysed and the cytoplasm content was decreased. Pathway analysis revealed that the APFD extract significantly affected biosynthesis peptidoglycan, gene expression, RNA processing, and macromolecule metabolism processes in S. mutans. Conclusion Data analysis revealed that multiple mechanisms of action were involved in antibacterial activity of A. paniculata extracts toward S. mutans.

A Dosing-Spoon-Based Electrochemical Sensor for Fast Assessment of Andrographis paniculata Extracts

Directly analyzing an herbal drug and its contamination is crucial to avoid severe problems due to uncertain dosages and unwanted contaminants. This article describes the first example of a dosing-spoon-based sensor for point-of-use detection of an herbal extract and its contamination. Andrographis paniculata is an herbal drug conventionally used for the therapy of many symptoms and infectious diseases, including COVID-19. The developed device consists of a sensitive electrochemical sensor, enabling the detection of A. paniculata extract. The lab-on-a-spoon squarewave voltammetric system provides the detection result with favorable sensitivity and simplicity in a short time (less than 15 s). The calibration curve of the modeled herbal extract covers a range of 0.030–7.350 mg ml–1 with a limit of detection of 20 μg ml–1. The device and data analysis were applied for determining real samples of A. paniculata extracts. The developed device is sensitive for the screening of processed products from A. paniculata. Moreover, this single dosing-spoon-based sensor can also simultaneously screen the contamination of heavy metals (i.e., cadmium and lead). We envision that this lab-on-a-spoon demonstration would expand the scope of portable pharmaceutical and biochemical sensors, offering promising convenient screening tools for industrial and household applications.