The pancreaticojejunostomy device is an exogenous object positioned at the junction between the jejunum and the pancreatic duct. Bacteria can readily settle on its surface, forming a biofilm, potentially leading to inflammation at the anastomotic site, resulting in overgrowth and narrowing of the anastomosis. Furthermore, biofilm formation on the inner surface of the lumen can obstruct the outflow duct of pancreatic fluid. This study aims to develop an innovative 3D pancreaticojejunostomy device coated with nano-silver for antibacterial properties. Chitosan serves as the coating medium, loaded with nano-silver particles. The presence of nano-silver in the coating is confirmed by observing its surface morphology and elemental composition and examining drug release patterns via inductively coupled plasma (ICP) analysis. Moreover, we validated its biocompatibility, antibacterial performance, and pancreas toxicity through in vitro and in vivo experiments. The results affirm the successful fabrication of this pancreaticojejunostomy device, highlighting its nano-silver coating and its antibacterial efficacy, as well as its satisfactory in vivo and in vitro biocompatibility and drug release capabilities. To sum up, this antibacterial 3D pancreaticojejunostomy device is promising to prevent infections and inflammatory reactions around the site and duct obstruction caused by bacterial biofilms.