Roads cause biodiversity loss and the effects of wildlife-vehicle collisions may ripple from individuals and populations to ecosystem functioning. Amphibians are threatened worldwide and, despite being particularly prone to roadkill impacts, they are often neglected in assessments. Here, we develop a sampling and analytical framework for spatially prioritizing mitigation actions for anuran amphibian roadkills based on fatality estimation and landscape conversion. The framework is composed of the six following steps: (1) pre-selection of segments to survey using the wetland coverage in the surroundings and the presence of roadkills of aquatic reptiles as a proxy for wet areas; (2) spatiotemporally replicated counts with a dependent double-observer protocol, that is, each segment is sampled multiple times by two pairs of people on foot; (3) extraction of covariates hypothesized to affect spatial and temporal variation in roadkill rates and persistence; (4) hierarchical open-population N-mixture modelling to estimate population dynamics parameters, which accounts for imperfect detection and spatiotemporal heterogeneity in removal, detection, and roadkill rates, and explicitly estimates carcass entries per time interval. (5) Assessment of land cover transition to infer landscape stability; and (6) prioritization of segments based on higher fatality rates and lower landscape conversion rates. We estimated a mean of 136 (95%CrI = 130–142) anurans roadkill per km per day in the 50 sample sites selected. The initial number of carcasses had a positive relationship with the percentage occupied by wetlands and a negative association with the percentage occupied by urban areas. The number of entrant carcass per interval was higher in the presence of rainfall and had a positive association with the wetlands cover. Carcass persistence probability was higher at night and lower in sites with high traffic volume. Ten segments (~1% of road extension) were prioritized using the median as threshold for fatality estimates and landscape conversion. It is urgent to appropriately evaluate the number of amphibians roadkilled aiming to plan and implement mitigation measures specifically designed for these small animals. Our approach accounts for feasibility (focused on sites with greater relevance), robustness (considering imperfect detection), and steadiness (less prone to loss of effectiveness due to landscape dynamics).
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