Pairwise Comparisons Research Articles

Validation of the pink esthetic score/white esthetic score at single tooth-supported prostheses in the esthetic zone: A randomized clinical trial.

To validate the reproducibility and inter/intra-observer variability of the Pink Esthetic Score/White Esthetic Score (PES/WES) of single tooth-supported prostheses in the maxillary esthetic zone (13-23). Forty-five patients were randomly assigned to one of the three treatment options (15 patients per group) receiving each one a different crown type: Porcelain fused to metal (PFM), monolithic zirconia, and lithium disilicate. Eight observers from each of four different specialties (Prosthodontists, Orthodontists, Periodontists, and Oral Surgeons) were recruited and assessed twice and four weeks apart (i.e., T1 and T2) 45 photographs of the single tooth-supported prosthesis using PES/WES and compared them with contralateral teeth. According to the ANOVA and post hoc tests, the zirconia crown type obtained the highest mean score by all observers, with a mean value of 16.70 ± 2.94. The prosthodontists and oral surgeons assigned the lowest mean score to PFM crowns, 13.03 ± 3.47 and 13.80 ± 3.17, respectively. Notably, the prosthodontists awarded the highest scores, specifically 17.50 ± 2.81 for the zirconia crowns. Intraobserver agreement was calculated utilizing the paired t-test. Pairwise comparisons between observers of different specialties revealed significant intraobserver agreement. Interclass correlation coefficient (ICC) scores were statistically significant among four specialties. No difference was detected concerning the interobserver agreement. The PES/WES index remains consistent across various observers from different specializations, yielding uniform results in the overall esthetic evaluation. Consequently, in light of the presented preliminary positive results, its use might also be considered for the esthetic assessment of single-tooth-supported prostheses. The PES/WES index may be employed clinically to evaluate single tooth-supported prostheses as it emerged as a reproducible esthetic scoring system.

The effectiveness of endoscopic diagnostics of colon tumors using artificial intelligence: prospective tandem study

AIM: to estimate the effectiveness of a medical decision support system based on artificial intelligence in the endoscopic diagnosis of benign tumors during tandem study.PATIENTS AND METHODS: from October to December 2023, a single-center comparative tandem study of medical decision support system based on artificial intelligence “ArtInCol” was done. The first stage was a traditional colonoscopy under sedation, the second one — colonoscopy using AI. A pairwise comparison of the main indicators of the effectiveness was made.RESULTS: in the primary endpoint, the polyp detection rate (PDR) in the traditional colonoscopy group was 40.6% vs 56.4% in the AI group, p = 0.0001 (RR = 1.39; 95% CI: 1.04–1.87). The mean number of lesions detected (MPP) was 1.63 (± 1.2) vs 2.47 (± 1.8) in the AI group (mean difference = 0.84; (95% CI: 1.07–0.61).CONCLUSION: the study demonstrated the effectiveness of the original medical decision support system for benign colon tumors detection in real clinical practice. The further stage, a multicenter randomized trial is needed.

My avatar makes me feel good? The effect of avatar personalisation and virtual agent interactions on self-esteem

ABSTRACT The theory of Objective Self-Awareness (OSA) and related studies suggest that embodiment of personalised avatars may induce self-awareness and influence self-esteem. Additionally, the Computers Are Social Actors (CASA) paradigm suggests that humans may mindlessly respond to computers in ways that are similar to human interactions. Based on those assertions, it is plausible that virtual embodiment of a personalised avatar and interactions with a virtual agent can shift self-esteem. However, those effects on self-esteem have not been thoroughly examined in past studies. To address these research gaps, a 2 (avatar personalisation: personalised vs. non-personalised avatar) × 2 (virtual agent interaction valence: positive vs. negative) between-subjects experiment was conducted using a Virtual Reality (VR) simulation (N =171). Findings from the study showed that there was no effects of avatar personalisation and virtual agent interaction valence on state self-esteem change. However, the pairwise comparisons present some preliminary indications that avatar personalisation and positive interactions with a virtual agent may facilitate improvements in state self-esteem altogether. Implications of the study findings are discussed.

A comparative evaluation of fracture resistance of three recent composite systems in Class II mesio occluso distal cavities - An in vitro study

Though composite resins have received popularity as restorative materials because of their physical properties, there was a dearth in the research about fracture resistance among recently emerged composite systems including Tokuyama Estelite Sigma Quick, Admira Fusion, and Beautifil II LS nanohybrid composite systems. To compare and evaluate the fracture resistance of maxillary premolars with class II mesio occluso distal cavities restored with Tokuyama Estelite Sigma Quick, Admira Fusion, and Beautifil II LS nanohybrid composite systems. Current invitro research was conducted on 44 intact human premolar teeth- Eleven specimens were kept completely intact for control group (Group G1). Class II MOD cavities were done with high-speed handpiece and under constant irrigation in remaining 33 teeth, Group G2, Group G3, Group G4 were restored with Tokuyama Estelite Sigma Quick (Tokuyama Dental, Tokyo, Japan.), Beautifil II LS (Shofu, Germany.), Admira Fusion (Shofu, Germany.) respectively. SPSS version 20 software to analyse the data; and ANOVA followed by Tukey’s post-hoc analysis to compare the fracture resistance were used. With a fracture resistance of 1297.27±10.27 N, Group 1 (Control) had highest fracture resistance. Group 4 has the least fracture resistance (Admira Fusion). Every pairwise comparison was significant too. Among the three materials studied, Beautifil II LS had higher fracture resistance and Admira Fusion had the least fracture resistance.

Variations in the anatomical structure of the pterion among populations from various global regions

Anatomical variations in the structure of the pterion were examined in various craniological series represent-ing modern populations from Australia, Melanesia, Southeast Asia, North Asia, America, and Europe, encom-passing 2035 adult crania. The comparison of populations based on the frequency of nonmetric traits revealed significant diversity across regions. Notably, the sphenoparietal connection is most frequent in European and South Asian populations, as well as among Native Americans, while it is least common among African groups. The frontotemporal connection predominates among Australian Aborigines and Melanesians and is also common in African populations but is almost non-existent in Europeans. The X-shaped pterion is most frequently observed in the Eskimos of Chukotka and the Chukchi, as well as among Somali and Tanzanian populations. A high fre-quency of epipteric bones is particularly characteristic of Southern Indians, Burmese, Australians, and Melane-sians. Pairwise comparisons of populations were conducted using Smith's Mean Measure of Divergence (MMD). To visualize the data from individual regions, the method of Multidimensional Scaling (MDS) was applied to the matrices of biological distances. The MDS plot for the North Asian region revealed distinct clusters of the Arctic, Baikal, Cen-tral Asian, and Ural groups, aligning with G.F. Debetz's classification based on morphological similarities among populations. In the MDS plot for the South and Southeast Asian, American, and African populations, Australians and Melanesians formed a separate cluster; Pueblo Indians were closely aligned with Peruvians, South Indians with Burmese, Malays merged with Papuans, and African populations were positioned relatively close to each other. In Europe, Caucasian groups formed a distinct cluster. On the combined graph, the populations were clearly divided by regions. The results obtained indicate that the spatial arrangement of populations on the graphs approximately mir-rors their genetic connections and shared origins. This suggests that nonmetric variations in the pterion area are more likely determined by genetic rather than ecological factors and can be considered taxonomically valuable traits at the regional level. Investigating the structure of the pterion in an evolutionary context may shed light on broader questions concerning the evolution of the skull and the mechanisms influencing its formation.

Matched case-control comparison of surgical success after XEN45 Gel Stent and PRESERFLO MicroShunt implantation in a Caucasian population.

The aim of this study was to compare the outcome of implantation of a XEN45 Gel Stent with the outcome of implantation of a Preserflo MicroShunt in a matched-pair analysis in eyes being naïve to filtering surgery. In this comparative, retrospective study, 50 eyes that had undergone XEN45 Gel Stent implantation were compared with 50 eyes after Preserflo implantation. Follow-up was at least 6 months, and surgical success was measured by criteria A (IOP < 21 mmHg, IOP reduction >20%, no repeat surgery); criteria B (IOP < 18 mmHg, IOP reduction >20%, no repeat surgery); and criteria C (IOP ≤15 mmHg, IOP reduction ≥40%, no repeat surgery). After a follow-up period of 12 months, mean IOP had decreased from preoperative 25.2 ± 4.8 mmHg in the XEN group to 14.5 ± 4.0 (n = 35) and from 25.3 ± 6.8 mmHg to 11.9 ± 2.9 (n = 41) in the Preserflo group, respectively. The IOP at the last follow-up of the two groups differed significantly (p < 0.01). The probability of surgical success did not differ concerning Criteria A and B, but surgical success was significantly higher in the Preserflo group for Criteria C (60%, p < 0.01). Both the Preserflo and XEN45 Gel Stent provide an effective and safe treatment option for advanced glaucoma and have a high potential to reduce intraocular pressure. Absolute IOP levels of <16 mmHg after 12 months were significantly more frequent in the Preserflo group.

Hip fractures in patients with primary aldosteronism - a Swedish nationwide study.

Previous studies suggest that primary aldosteronism (PA) is associated with dysregulated bone homeostasis. The aim of this study was to evaluate the incidence of hip fractures in patients with PA. We studied a nationwide cohort of 2419 patients with PA (1997-2019) and 24187 age and sex matched controls from the general population. Hip fractures were identified by ICD codes in the Swedish National Patient Register. We estimated hazard ratios (HRs) for incident hip fractures, adjusted for prior fractures, socioeconomic factors, diabetes, osteoporosis, hyperparathyroidism, and cardiovascular disease (CVD). Pairwise subgroup comparisons were performed by age (18-56 and > 56years), sex, CVD at baseline, and treatment for PA. During a mean follow up of 8 ± 5 years, 64 (2.6%) patients had a hip fracture after being diagnosed with PA, compared to 401 (1.7%) controls. After adjustments, PA was associated with a 55% increased risk of hip fracture compared to controls (HR 1.55 [1.18-2.03]). HRs were increased in women (HR 1.76 [95% CI 1.24-2.52]), patients aged > 56 years (HR 1.62 [95% CI 1.21-2.17]), and patients with CVD at diagnosis (HR 2.15 [95% CI 1.37-3.37]). PA patients treated with adrenalectomy did not have higher risk than controls (HR 0.84 [95% CI 0.35-2.0]), while patients treated with mineralocorticoid receptor antagonists (MRA) retained a greater risk (HR 1.84 [95% CI 1.20-2.83]). PA is associated with increased hip fracture risk, especially in women, patients diagnosed after the age of 56 years and patients with established CVD at diagnosis. Also, patients treated with MRA seem to have an increased risk of hip fractures, while adrenalectomy may be protective.

Case-Case Genome-Wide Analyses Identify Subtype-Informative Variants that Confer Risk for Breast Cancer.

Breast cancer includes several subtypes with distinct characteristic biological, pathological, and clinical features. Elucidating subtype-specific genetic etiology could provide insights into the heterogeneity of breast cancer to facilitate development of improved prevention and treatment approaches. Here, we conducted pairwise case-case comparisons among five breast cancer subtypes by applying a case-case GWAS (CC-GWAS) approach to summary statistics data of the Breast Cancer Association Consortium. The approach identified 13 statistically significant loci and eight suggestive loci, the majority of which were identified from comparisons between triple-negative breast cancer (TNBC) and luminal A breast cancer. Associations of lead variants in 12 loci remained statistically significant after accounting for previously reported breast cancer susceptibility variants, among which two were genome-wide significant. Fine mapping implicated putative functional/causal variants and risk genes at several loci, e.g., 3q26.31/TNFSF10, 8q22.3/NACAP1/GRHL2, and 8q23.3/LINC00536/TRPS1, for TNBC as compared to luminal cancer. Functional investigation further identified rs16867605 at 8q22.3 as a SNP that modulates enhancer activity of GRHL2. Subtype-informative polygenic risk scores (PRS) were derived, and patients with a high subtype-informative PRS had an up to 2-fold increased risk of being diagnosed with TNBC instead of luminal cancers. The CC-GWAS PRS remained statistically significant after adjusting for TNBC PRS derived from traditional case-control GWAS in The Cancer Genome Atlas and the African Ancestry Breast Cancer Genetic Consortium. The CC-GWAS PRS was also associated with overall survival and disease-specific survival among breast cancer patients. Overall, these findings have advanced our understanding of the genetic etiology of breast cancer subtypes, particularly for TNBC.

Effects of psychological interventions on fear of cancer recurrence: A systematic review and network meta‐analysis

AbstractThe study aimed to systematically compare the effects of psychological interventions on relieving fear of cancer recurrence (FCR) by a systematic review and network meta‐analysis (NMA). The relevant randomized controlled trials were searched from China National Knowledge Infrastructure, Wanfang Database, VIP Database for Chinese Technical Periodicals, Chinese Biomedical Literature Database, Cochrane Library, Pubmed, Web of Science, CINAHL, PsycINFO, and Embase. The retrieval time was from the establishment of each database to January 23, 2024. Review Manager 5.4 software was used to evaluate the quality of each literature that met the inclusion and exclusion criteria. Stata16.0 was used for NMA. Standardized mean differences (SMDs) of patients' FCR outcomes and 95% confidence intervals (CIs) were used to determine the effects. Inconsistency test, network map, surface under the cumulative rankings curve (SUCRA), comparison‐adjusted funnel plot were performed. A total of 41 articles were included, with 4056 patients and 15 psychological interventions. Six psychological interventions (NT, Narrative Therapy; ACT, Accept and Commitment Therapy; GT, Therapy based on Gratitude‐Expanded Behavior Theory; Blend Cognitive Behavior Therapy; PERMA, PERMA Therapy; CBT, Cognitive Behavior Therapy) were effective in alleviating FCR in the short term compared with usual care, whereas the effects of ACT, GT, and CBT were sustained up to more than 3 months postintervention. NT ranked as most likely to alleviate FCR, (SUCRA: 89.8%, SMD: −2.89, 95% CI: −4.08 to −1.69), followed by ACT (SUCRA: 88.1%, SMD: −2.83, 95% CI: −4.38 to −1.27) in short‐term effects. GT ranked as most likely to alleviate FCR in long‐term effects (SUCRA: 100%, SMD: −3.35, 95% CI: −4.21 to −2.50), followed by ACT (SUCRA: 88.9%, SMD: −1.64, 95% CI: −2.36 to −0.91). However, most of the quality of evidence for pairwise comparison was rated as “very low” to “low.” The evidence can help inform evidence‐based practice and guide healthcare providers in deciding on the most effective psychological interventions for FCR, which should also be viewed with caution due to the low level of the quality.

Solar energy potential mapping in Ukraine through integration of GIS, remote sensing, and fuzzy logic

ABSTRACT The Green Deal strategic plan for the development of renewable energy until 2030 is of particular importance in the context of the restoration of Ukraine’s post-war energy infrastructure. One of the key topics is the analysis of the possibilities of installing large solar power plants in Ukraine. In this article, a multi-criteria analysis of the suitability of the territory of Ukraine is carried out on the basis of climatic, topographic and land use criteria. To assess land suitability, criteria standardized using fuzzy logic with weights determined by experts through the method of pairwise comparisons were combined using a weighted sum model. Upon completing the study, a suitability map was generated, depicting zones with varying levels of suitability (ranging from 0 to 1) for solar power plant placement. It was found that more than 35.68% of the country has average values of the suitability index (0.65–0.7), and approximately 18.82% show high indicators (<0.7). Conditions are especially favorable in the south of Ukraine.

A case series of fluoroscopy-guided neurolytic splanchnic nerve block for chronic pancreatitis pain

Context: Splanchnic nerve block (SPN) with local anesthetic and steroid is used to relieve the pain of chronic pancreatitis. However, it does not provide long-lasting relief. We hypothesize that the use of 70% alcohol will give adequate analgesia for more than months. Aims: The primary objective was to find out the analgesic efficacy of the use of 70% alcohol with SPN. Secondary objectives included the incidence of side effects, analgesic consumption postintervention, quality of life (QOL) assessed via a self-reported quality of life scale, and repeat block during the 1-year follow-up period. Settings and Design: Retrospective analysis of all patients with chronic pancreatitis who received bilateral SPN over the last 4 years. Methods and Materials: SPN was performed using the posterior retrocrural approach with the patient in the prone position as described in the literature using a 23 G × 90 mm spinal needle bilaterally at the level of T12 using C-arm/fluoroscopy guidance. Data were collected from the procedure book of the pain clinic and medical records. Statistical Analysis Used: Quantitative data for change in pre- to post-block VAS score was collected using the non-parametric Wilcoxon signed ranks test. Results: The baseline VAS, post-procedure VAS, and VAS at 3 months follow-up was 7.69 ± 1.3,2.44 ± 0.96 and 1.56 ± 1.15. A pairwise comparison of VAS performed between baseline and immediate post-procedure, baseline, and VAS at 3 months was found to be highly significant. Conclusions: Fluoroscopy-guided neurolytic SPN with 70% alcohol gives significant pain relief for more than 3 months. It also leads to improvement in 3 months QOL.

Strategic Management of Financial and Economic Security: Modern Challenges and Threats in a Hyperdynamic External Environment

The main purpose of the article is to increase the efficiency of strategic management of the financial and economic security of a modern enterprise in the face of new challenges and threats. The object of the study is the system of financial and economic security of a modern company. The scientific task is to assess modern surpluses and threats in the system of ensuring the financial and economic security of an enterprise. The research methodology involves the use of modern methods of system analysis, pairwise comparison and hierarchical analysis to assess challenges and threats. As a result, a matrix of the most significant challenges and threats in the system of ensuring the financial and economic security of a modern enterprise was built. The innovativeness of the results obtained is revealed through the proposed methodological approach to assessing and streamlining challenges and threats in the system of ensuring the financial and economic security of a modern enterprise.

Effect of different inhalant allergens on T-cell subsets in adults with bronchial asthma

Objective We analyzed the impact of different inhalant allergens on T-lymphocyte subsets in patients diagnosed with bronchial asthma. Methods The study included 57 bronchial asthma patients and 22 healthy controls. Asthma patients were categorized into dust mite, animal hair, pollen, and mold groups. Flow cytometry was used to measure the cells in the case group and control group. These T-lymphocyte subset markers were evaluated among patients with bronchial asthma caused by different allergens as well as between the case group and control group. Results Peripheral blood CD4+ T-cells, CD8+ T-cells, CD4/CD8 ratio, and Th17/Treg ratios were all higher in the case group than in the control group (p < 0.05). Peripheral blood T-lymphocyte subsets were compared among the four groups, and it was found that there were statistical differences in the Th17/Treg ratio among the four groups (p < 0.05). There were no significant differences observed among the four groups in terms of CD3+ cells, CD4+ cells, CD8+ cells, Th1 cells, Th2 cells, Th17 cells, Treg cells, Th9 cells, and Th22 cells. Further pairwise comparison was made, and the results suggested that the peripheral blood Th17/Treg ratio in the pollen mixed group was lower than that in the dust mite mixed group, animal hair mixed group, and mold mixed group (p < 0.05). Conclusion Patients with bronchial asthma show varied T-lymphocyte subset responses to different inhalant allergens. Elevated CD4+ T cells and Th17 cells in peripheral blood could indicate asthma risk. However, small sample size may introduce bias to these findings.

Comparison of the Extent of Root Resorption in Maxillary Anterior Teeth Retracted using Regular Temporary Anchorage Devices (TADS) Versus Conventional Anchorage Methods: A Retrospective Study

Aim The aim of this study will be to compare the amount of root resorption of maxillary anterior retracted with anterior TADs,regular TADs and without skeletal anchorage.  Materials and methods: Samples for the study will be the patients undergone orthodontic treatment at the Department of Orthodontics,K.V.G. Dental College and Hospital, Sullia Sample size of 45 patients will be divided in to 3 groups . ,Group 1 consists of patients treated with anterior TADs, Group2 consists of patients treated with regular TADs and Group 3 consists of patients treated without skeletal anchorage for retraction of maxillary anteriors. Root resorption will be measured by comparing pretreatment and post treatment intraoral periapical radiographs (IOPAR) radiographs.  Result- The study meticulously compared root resorption levels among three groups: Conventional, Anterior Temporary Anchorage Devices (TADs), and Posterior TADs. Anterior TADs exhibited the highest mean root resorption, significantly higher than both Conventional and Posterior TADs. Statistical analyses confirmed these differences, highlighting the impact of anchorage method on root resorption. Pairwise comparisons and confidence intervals further supported the findings, emphasizing the nuanced variations observed.

Hypogonadism in men with inflammatory joint diseases: Frequency and clinical characteristics

To study the frequency of hypogonadism (HG) in men with rheumatoid arthritis (RA), ankylosing spondylitis (AS) and psoriatic arthritis (PsA) and to evaluate the impact of HG on the course of RA and and concomitant diseases. A single-stage continuous study included 170 men with RA, 57 men with AS and 85 men with PsA, who were hospitalized at the Nasonova Research Institute of Rheumatology. Patients were assessed for total testosterone (ТS) levels and subsequently divided into subgroups with normal (>12 nmol/l) and reduced levels. An intergroup comparison was carried out on the main indicators used in clinical rheumatological practice to assess the stage, activity and other medical and demographic characteristics of rheumatic disease, as well as on concomitant conditions. The second stage of the study involved a pairwise intergroup comparison among patients with HG with RA, AS and PsA. The incidence of ТS deficiency among patients with RA was 24.1%, among patients with AS - 17.5%, and with PsA - 31.8%. In patients with RA, HG was associated with a significantly higher mean body mass index, higher fasting blood glucose and uric acid, higher erythrocyte sedimentation rate and anemia. Patients with AS with HG had significantly lower hemoglobin levels and more frequent anemia, as well as higher levels of C-reactive protein and erythrocyte sedimentation rate. In PsA, older age was observed in the androgen deficiency group, as well as higher body mass index and fasting glucose levels; obesity was more common. An intergroup comparison of quantitative and qualitative indicators between patients with androgen deficiency in all three rheumatic diseases (RDs) did not reveal significant differences in the average concentrations of ТS, luteinizing hormone, sex hormone binding globulin, experience of RD, laboratory markers of inflammatory activity, as well as glucose and uric acid. A similar incidence of diabetes mellitus, obesity and anemia was noted for all three nosologies. ТS levels and the presence of HG were not associated with the stage and activity of RD, but ТS deficiency was accompanied by higher laboratory indicators of inflammatory activity, lower hemoglobin values, and metabolic disorders. Patients with HG, regardless of nosology, had similar levels of sex hormones and indicators reflecting RD and concomitant conditions.

Alternative Virtual Lab-Based Practical Learning Model to Improve Scientific Attitude and Science Process Skills

A virtual laboratory is computer software that has the ability to perform mathematical modeling of computer devices presented in the form of simulations. Virtual laboratories are not a substitute for real laboratories, but are used to complement and improve the weaknesses of real laboratories. This research aims to determine the effect of virtual labs on science education students' scientific attitude abilities and science process skills in science courses. The design of this research is a repeated pre-experiment without a control class, with a one-group and pretest-posttest design. The sample in this study was 60 students of the science education study program at Makassar State University, Indonesia, used as experiment 1 group A (n = 30), experiment 2 group B (n = 30). Two groups (experiment 1 and experiment 2) were tested with a pretest and posttest. Each group uses a virtual lab-based practical model (PbVLab). Scientific attitudes and scientific skills were measured using multiple choice tests before and after treatment. The mixed method ANOVA statistical test was used to determine how effective the use of PbVLab was in improving scientific attitudes and science process skills. The results of testing research based on Within-Subjects Effects showed that there was no difference between the pretest-posttest scores for scientific attitudes and scientific skills (F = 0.00; p &lt; 0.05) in each group. The results of the pairwise comparison showed a significance value of &lt;0.05, and there was a significant increase in the pretest-posttest scores for scientific attitudes and science process skills in each group. The effect size results (partial eta squared) show that experimental group 1 experienced an increase in scientific attitudes by 52%; science process skills 53%, experimental group 2 scientific attitudes 54% and science process skills 56%. So, it can be concluded that experimental group 1 and experimental group 2 (PbVab model) provide the most effective contribution to improving scientific attitudes and science process skills.

Necessary to continue imaging spontaneous cervical artery dissecting pseudoaneurysms after one year?

Background and purposeThe value of long-term serial imaging of dissecting pseudoaneurysm (dPSA) is poorly characterized. This study investigated the long-term radiographic evolution of dPSA. MethodsWe performed a query in our institutional craniocervical artery dissection registry to identify cases with spontaneous dPSA who had at least one year of follow-up with serial angiographic imaging. We performed Wilcoxon rank-sum pairwise comparison test to determine if there was a significant change in the aneurysm size over time. ResultsThis observational cohort study included 76 patients (46 females; 64 dPSA in the internal carotid artery [ICA] and 12 in the vertebral artery [VA]) with a median age of 49.5 years (range 24-77). The initial median dPSA size was 8 mm (interquantile range(iqr) = 5.88-11mm), and the final median dPSA size was 7 mm (iqr = 4-11 mm). Most patients had either no change or reduction in dPSA size in the serial follow-up, with no significant change over time. All the patients had favorable outcomes at the last follow-up, and most patients were symptom-free from dPSA (92 %). Two patients (2.6%) experienced recurrent ischemic strokes in the same territory as the initial ischemic stroke without any change in dPSA size. ConclusionFurther serial scans for dPSA after one year may be deferred in the absence of interim clinical symptoms as most dPSA either remains stable or decreases in size. Recurrent stroke, although a rare event, was not associated with an increase in dPSA size.

Proteomic signatures uncover phenotypic plasticity of susceptible and resistant genotypes by wall remodelers in rice blast.

Molecular communication between macromolecules dictates extracellular matrix (ECM) dynamics during pathogen recognition and disease development. Extensive research has shed light on how plant immune components are activated, regulated and function in response to pathogen attack. However, two key questions remain largely unresolved: (i) how does ECM dynamics govern susceptibility and disease resistance, (ii) what are the components that underpin these phenomena? Rice blast, caused by Magnaporthe oryzae adversely affects rice productivity. To understand ECM regulated genotype-phenotype plasticity in blast disease, we temporally profiled two contrasting rice genotypes in disease and immune state. Morpho-histological, biochemical and electron microscopy analyses revealed that increased necrotic lesions accompanied by electrolyte leakage governs disease state. Wall carbohydrate quantification showed changes in pectin level was more significant in blast susceptible compared to blast resistant cultivar. Temporally resolved quantitative disease- and immune-responsive ECM proteomes identified 308 and 334 proteins, respectively involved in wall remodelling and integrity, signalling and disease/immune response. Pairwise comparisons between time and treatment, messenger ribonucleic acid expression, diseasome and immunome networks revealed novel blast-related functional modules. Data demonstrated accumulation of α-galactosidase and phosphatase were associated with disease state, while reactive oxygen species, induction of Lysin motif proteins, CAZymes and extracellular Ca-receptor protein govern immune state.

Vision Experiments on Chroma-Related Preference for Color Quality of Lighting

ABSTRACT Two vision experiments were conducted to quantify the chroma-related preference in color quality of lighting in a real room-size cubicle simulating interior lighting environment with spectrally tunable light sources. The first experiment used 9 different lights with increased or decreased chroma in nine levels in red-green directions. The second experiment used 11 different lights with increased or decreased chroma in three hue directions, for red, green, and yellow, controlled as independently as possible. About 20 subjects participated in these two experiments evaluating the appearance of various fruits and vegetables as well as skin tone under these test lights by numerous pair comparisons. The results of the first experiment showed that chroma increase in ΔC*ab ≈ 4 to 8 from the reference light was most preferred for all target objects, and desaturated lights were disliked in all cases. The second experiment showed that chroma change in red is significantly important for the preference, green was second, and yellow had the least effect on preference. The results of the second experiment suggested that preference increases with increasing red chroma in light, and after a peak in preference (between ΔC*ab ≈ 5 to 10), preference starts decreasing, which is consistent with findings from the first experiment. Neither the viewing targets nor the correlated color temperature (CCT) conditions were found to have significant impact on the subjects’ preference.

CREB5 as an essential transcription and tumorigenic regulator in basal-like breast and prostate cancer.

1107 Background: Breast cancers (BC) harbor basal-like transcriptional profiles, in which a large subset are pathologically triple negative breast cancers (TNBC). These are hormone therapy insensitive, have limited targeted therapies, and decreased overall survival (OS). Recent studies determined that prostate cancers (PCs) also exhibit luminal- or basal-like transcriptional subtypes. The 30-40% of basal-like PCs exhibit resistance to hormone therapies and have overall worse clinical outcomes. Here we deployed in silico computational models and tumor phenotype testing in vitro and in vivo. This identified that CREB5, a transcription factor commonly amplified or overexpressed in advanced cancers, regulates basal-like BCs and PCs through stem cell-like genes and properties that drive tumor formation. Methods: Protein and RNA levels were examined in 5 subtypes of BC from the TCGA (n=981). Normalized expression and PAM50 signatures were analyzed using data from Decipher tests of PC patients (n=585). Kaplan-Meier curves were used to compare relapse free survival in PC (n=84) and OS in TNBC (n=248). The ALAN (Algorithm for Linking Activity Networks (1) computational tool was used to determine gene-gene similarities of ~20,000 genes based on ~400,000,000 pairwise comparisons in 208 tumors. In PC (LNCaP) and TNBC (MDA-MB-231 and HCC1806) cell lines, we examined gene expression profiles from RNA-seq and found Hallmark signatures through Gene Set Enrichment Analysis. Motif analysis was conducted from ChIP-seq data on immuno-precipitated CREB5. We conducted in vitro and in vivo assessment of tumorigenicity. Results: Basal BCs harbored increased CREB5 expression and CREB5-high basal BCs had worse outcomes (HR 2.09, 95% CI 1.1-3.96, p&lt;0.021). Reflecting this, in basal-like BC cell lines, CREB5 increased cell viability and formation of tumor-like spheroids in 3D culture. In PCs, CREB5 had a positive correlation with basal- and not luminal-like PCs (Spearman ρ = 0.519, p&lt;0.001). Through ALAN analysis of metastatic PCs, CREB5 exhibited strong concordance with other known resistance genes (FGFR1/2) as well as transcription factors that promote stem cell-like features. Particularly, CREB5 was associated with increased FOSL1 (Spearman ρ = 0.37, p&lt;0.001), a metastasis-regulating gene. In PC cell lines, CREB5 overexpression promoted FOSL1 and other stem cell-like genes and co-bound to FOSL1 transcription regulatory elements. CREB5 overexpression in PC cell lines promoted the formation of tumor-like colonies, spheroids, and increased tumor formation from the xenografts in mice. Conclusions: Basal-like BCs and PCs demonstrate overexpression of CREB5, a gene that promotes tumor formation and stem cell-like genes. This mechanistic knowledge may guide treatment management strategies and aid in the development of novel therapies against basal-like BCs and PCs. 1. Bergom et al, Communications Biology, 2023.