Event Abstract Back to Event Electrophysiological Pain Responses in Cultured Human iPSC-Derived Sensory Neurons Using High-Throughput Multi-Electrode Array System Aoi Odawara1, 2, 3, Naoki Matsuda1 and Ikuro Suzuki1, 4, 5* 1 Tohoku Institute of Technology, Department of Electronics, Japan 2 Tohoku University, AIMR, Japan 3 Japan Society for the Promotion of Science (JSPS), Japan 4 Japan Agency for Medical Research and Development (AMED), Japan 5 Consortium for Safety Assessment using Human iPS Cells (CSAHi), Japan Dorsal root ganglion (DRG) sensory neurons are pain-related neurons and have a variety of sensory receptors that are activated by chemical, thermal, and mechanical stimuli. Establishment of pharmacological assay in pain research and drug screening is important issue. In addition, human induced pluripotent stem cell (hiPSC)-derived sensory neurons may be effectively used for drug discovery and toxicity testing. The purpose of this study was to evaluate the physiological responses against typical pain-related molecules, temperature change and anti-cancer drug in cultured sensory neurons using high-throughpupt multi-electrode array (MEA) system. Human iPSC-derived sensory neurons were cultured on MEA chips, and the electrophysiological responses against capsaicin, menthol, allyl isothiocyanate (AITC), anti-cancer drug vincristine and oxaliplatin were measured by the MEA system. To confirm the responses depending on each receptor, we examined the responses in presence of each receptor antagonist. We also examined whether the increase of cold sensitivities occur in presence of anticancer drug in vitro hiPSC-derived sensory neurons. We firstly confirmed the expression of typical sensory marker Nav1.7, TRPV1, TRPM8, and TRPA1 using immunostaining in culture hiPSC-derived sensory neurons at 8 weeks culture. Evoked responses against capsaicin, menthol, and AITC were detected. As the responses disappeared with each channle bloker, these responses were confirmed to be channel specific responses. The evoked responses against anticancer drug vincristine and oxaliplatin were also detected. Furthermore, we found that the responses against AITC increase in presence oxaliplatin and with the concentration of oxaliplatin. We have succeeded in detecting the electrophysiological pain reponses against capsaicin, menthol, allyl isothiocyanate (AITC), anti-cancer drug vincristine and oxaliplatin in hiPSC-derived sensory neurons using MEA system. We found that the increase of cold sensitivities in vivo phenomenon was also detected in vitro hiPSC-derived sensory neurons. Keywords: hiPSC-derived sensory neurons, TRP channels, Anti-cancer drug, Pharmacology, High-Throughput MEA system Conference: MEA Meeting 2018 | 11th International Meeting on Substrate Integrated Microelectrode Arrays, Reutlingen, Germany, 4 Jul - 6 Jul, 2018. Presentation Type: Poster Presentation Topic: Stem cell-derived applications Citation: Odawara A, Matsuda N and Suzuki I (2019). Electrophysiological Pain Responses in Cultured Human iPSC-Derived Sensory Neurons Using High-Throughput Multi-Electrode Array System. Conference Abstract: MEA Meeting 2018 | 11th International Meeting on Substrate Integrated Microelectrode Arrays. doi: 10.3389/conf.fncel.2018.38.00060 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 18 Mar 2018; Published Online: 17 Jan 2019. * Correspondence: Prof. Ikuro Suzuki, Tohoku Institute of Technology, Department of Electronics, Sendai, Japan, s.ikurou@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Aoi Odawara Naoki Matsuda Ikuro Suzuki Google Aoi Odawara Naoki Matsuda Ikuro Suzuki Google Scholar Aoi Odawara Naoki Matsuda Ikuro Suzuki PubMed Aoi Odawara Naoki Matsuda Ikuro Suzuki Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.