P-uptake Efficiency Research Articles

Interactive effects of phosphorus fertilization and salinity on plant growth, phosphorus and sodium status, and tartrate exudation by roots of two alfalfa cultivars.

Soil phosphorus (P) deficiency and salinity are constraints to crop productivity in arid and semiarid regions. Salinity may weaken the effect of P fertilization on plant growth. We investigated the interactive effects of soil P availability and salinity on plant growth, P nutrition and salt tolerance of two alfalfa (Medicago sativa) cultivars. A pot experiment was carried out to grow two cultivars of alfalfa in a loess soil under a combination of different rates of added P (0, 40, 80 and 160 mg P kg-1 soil as monopotassium phosphate) and sodium chloride (0, 0.4, 0.8 and 1.6 g NaCl kg-1 soil). Plant biomass, concentrations of P ([P]), sodium ([Na]) and potassium ([K]) were determined, and rhizosheath carboxylates were analysed. There were significant interactions between soil P availability and salinity on some, but not all, of the parameters investigated, and interactions depended on cultivar. Plant growth and P uptake were enhanced by P fertilization, but inhibited by increased levels of salinity. Increasing the salinity resulted in decreased plant P-uptake efficiency and [K]/[Na]. Only soil P availability had a significant effect on the amount of tartrate in the rhizosheath of both cultivars. Increased salinity aggravated P deficiency. Appropriate application of P fertilizers improved the salt tolerance of alfalfa and increased its productivity in saline soils.

Cultivar-Dependent Responses in Plant Growth, Leaf Physiology, Phosphorus Use Efficiency, and Tuber Quality of Potatoes Under Limited Phosphorus Availability Conditions.

The limited availability of phosphorus (P) in soils causes a major constraint in the productivity of potatoes, which requires increased knowledge of plant adaptation responses in this condition. In this study, six potato cultivars, namely, Agria, Lady Claire, Milva, Lilly, Sieglinde, and Verdi, were assessed for their responses on plant growth, leaf physiology, P use efficiency (PUE), and tuber quality with three P levels (Plow, Pmed, and Phigh). The results reveal a significant variation in the cultivars in response to different P availabilities. P-efficient cultivars, Agria, Milva, and Lilly, possessed substantial plant biomass, tuber yield, and high P uptake efficiency (PUpE) under low P supply conditions. The P-inefficient cultivars, Lady Claire, Sieglinde, and Verdi, could not produce tubers under P deprivation conditions, as well as the ability to efficiently uptake P under low-level conditions, but they were efficient in P uptake under high soil P conditions. Improved PUpE is important for plant tolerance with limited P availability, which results in the efficient use of the applied P. At the leaf level, increased accumulations of nitrate, sulfate, sucrose, and proline are necessary for a plant to acclimate to P deficiency-induced stress and to mobilize leaf inorganic phosphate to increase internal PUE and photosynthesis. The reduction in plant biomass and tuber yield under P-deficient conditions could be caused by reduced CO2 assimilation. Furthermore, P deficiency significantly reduced tuber yield, dry matter, and starch concentration in Agria, Milva, and Lilly. However, contents of tuber protein, sugars, and minerals, as well as antioxidant capacity, were enhanced under these conditions in these cultivars. These results highlight the important traits contributing to potato plant tolerance under P-deficient conditions and indicate an opportunity to improve the P efficiency and tuber quality of potatoes under deficient conditions using more efficient cultivars. Future research to evaluate molecular mechanisms related to P and sucrose translocation, and minimize tuber yield reduction under limited P availability conditions is necessary.

Genetic Dissection of Phosphorous Uptake and Utilization Efficiency Traits Using GWAS in Mungbean

Mungbean (Vignaradiata L. Wilczek) is an early maturing legume grown predominantly in Asia for its protein-rich seeds. P deficiency can lead to several physiological disorders which ultimately result in a low grain yield in mungbean. The genetic dissection of PUpE (Puptake efficiency) and PUtE (P utilization efficiency) traits are essential for breeding mungbean varieties with a high P uptake and utilization efficiency. The study involves an association mapping panel consisting of 120 mungbean genotypes which were phenotyped for total dry weight, P concentration, total P uptake, and P utilization efficiency under low P (LP) and normal P (NP) conditions in a hydroponic system. A genotyping-by-sequencing (GBS) based genome-wide association study (GWAS) approach was employed to dissect the complexity of PUpE and PUtE traits at the genetic level in mungbean. This has identified 116 SNPs in 61 protein-coding genes and of these, 16 have been found to enhance phosphorous uptake and utilization efficiency in mungbeans. We identified six genes with a high expression (VRADI01G04370, VRADI05G20860, VRADI06G12490, VRADI08G20910, VRADI08G00070 and VRADI09G09030) in root, shoot apical meristem and leaf, indicating their role in the regulation of P uptake and utilization efficiency in mungbean. The SNPs present in three genes have also been validated using a Sanger sequencing approach.

Effects of various phosphorus fertilizers on maize yield and phosphorus uptake in soils with different pH values

ABSTRACT The performance of fertilizers may differ significantly in different soils. Here, we quantify effects of various phosphorus (P) fertilizers on maize yield and P uptake in soils with different pH values, using a pot experiment. Five P fertilizers were used; monoammonium phosphate (MAP), diammonium phosphate (DAP), calcium superphosphate (CSP), calcium magnesium phosphate (CMP) and, ammonium polyphosphate (APP). Two soils were neutral fluvo-aquic soil (pH 7.2) and acidic yellow cinnamon soil (pH 5.0). Generally, acidic soil restricted the root activity, dry matter accumulation, P uptake, and yield of maize. The effects of P fertilizers were inconsistent in the different soils. In neutral soil, the effects were the highest for DAP and lowest for CMP. However, in acidic soil they were highest for CMP, followed by DAP, and lowest for MAP. In neutral soil, DAP treatment showed higher P uptake efficiency (PUpE) and partial factor productivity (PPFP), while CMP treatment showed higher P harvest index (PHI) and utilization efficiency PUtE. In acidic soil, the PUpE and PPFP of CMP and DAP treatments were higher than in other treatments. Therefore, choosing the right P fertilizer based on soil acidity can significantly improve maize yield, P uptake, and P utilization to optimize P management.

Phosphorus and selenium uptake, root morphology, and carboxylates in the rhizosheath of alfalfa (Medicago sativa) as affected by localised phosphate and selenite supply in a split-root system.

Low availability of phosphorus (P) is a key limiting factor for the growth of many crops. Selenium (Se) is a nutrient for humans that is acquired predominantly from plants. Localised P and Se supply may affect P- and Se-uptake efficiency. Our aim was to examine the mechanisms of alfalfa (Medicago sativa L.) to acquire P and Se when the elements are heterogeneously or homogeneously distributed in soil, and how P and Se supply affect plant growth and uptake of P and Se. We conducted a split-root experiment growing alfalfa in a loess soil with two distribution patterns (i.e. heterogeneous and homogeneous) of P and Se. The application rates of P (KH2PO4) and Se (Na2SeO3) were 0 and 20mgPkg-1, and 0 and 1mgSekg-1, respectively. Our results showed that plants absorbed more Se when both P and Se were supplied homogeneously than when supplied heterogeneously. Supplying Se had a positive effect on plant P content. Localised P supply resulted in the exudation of more carboxylates by roots than homogeneous P supply did. Soil microbial biomass P was significantly greater when P was supplied homogeneously. Shoot-to-root translocation of Se had a positive effect on P-uptake efficiency. These results indicated that, compared with homogeneous P supply, localised P supply promoted P and Se uptake by increasing the amount of rhizosheath carboxylates and weakening the competition between roots and microbes. Translocation of Se within plant organs was promoted by the application of P, thus enhancing the P-uptake efficiency of alfalfa.

Dolomite and Micronutrient Fertilizer Affect Phosphorus Fate When Growing Crape Myrtle in Pine Bark

Soilless substrates are routinely amended with dolomite and sulfate-based micronutrients to improve fertility, but the effect of these amendments on phosphorous (P) in substrate pore-water during containerized crop production is poorly understood. The objectives of this research were as follows: compare the effects of dolomite and sulfate-based micronutrient amendments on total P (TP), total dissolved P (TDP), orthophosphate P (OP), and particulate P (PP; TP − TDP) concentrations in pour-through extracts; to model saturated solid phases in substrate pore-water using Visual MINTEQ; and to assess the effects of dolomite and micronutrient amendments on growth and subsequent P uptake efficiency (PUE) of Lagerstroemia L. ‘Natchez’ (crape myrtle) potted in pine bark. Containerized crape myrtle were grown in a greenhouse for 93 days in a 100% pine bark substrate containing a polymer-coated 19N–2.6P–10.8K controlled-release fertilizer (CRF) and one of four substrate amendment treatments: no dolomite or micronutrients (control), 2.97 kg·m−3 dolomite (FL); 0.89 kg·m−3 micronutrients (FM); or both dolomite and micronutrients (FLM). Pour-through extracts were collected approximately weekly and fractioned to measure pore-water TP, TDP, and OP and to calculate PP. Particulate P concentrations in pour-through extracts were generally unaffected by amendments. Relative to the control, amending pine bark with FLM reduced water-extractable OP, TDP, and TP concentrations by ≈56%, had no effect on P uptake efficiency, and resulted in 34% higher total dry weight (TDW) of crape myrtle. The FM substrate had effects similar to those of FLM on plant TDW and PUE, and FM reduced pore-water OP, TDP, and TP concentrations by 32% to 36% compared with the control. Crape myrtle grown in FL had 28% lower TDW but pour-through OP, TDP, and TP concentrations were similar to those of the control. Chemical conditions in FLM were favorable for precipitation of manganese hydrogen phosphate (MnHPO4), which may have contributed to lower water-extractable P concentrations in this treatment. This research suggests that amending pine bark substrate with dolomite and a sulfate-based micronutrient fertilizer should be considered a best management practice for nursery crop production.

Agronomic assessment of phosphorus efficacy for potato (Solanum tuberosum L) under legume intercrops

Phosphorus (P) is an essential element and its efficient use is of global importance. This study evaluated the effect of growing potato under legume intercrops on P uptake and use efficiency indices: P harvest index (PHI), P uptake efficiency (PuPE), P partial factor productivity (PPFP) and P partial balance (PPB). The experiment was carried out for four consecutive seasons with treatments comprising potato cultivated under legume intercrops: none (T1), dolichos (Lablab purpureus L) (T2), peas (Pisum sativum L) (T3) and beans (Phaseolus vulgaris L) (T4). Across the seasons, the mean haulm P uptake for T2 (6.7 kg P ha−1), T4 (5.5) and T3 (4.5) were 6%, 23% and 36% lower than that observed in T1 (7.1 kg P ha−1), respectively. On the other hand, tuber P uptake was highest in T1 (21.8 kg P ha−1) and T2 (21.3 kg P ha−1) and were significantly higher than 13.2 kg P ha−1 in T3 and 15.1 kg P ha−1 in T4. This had a profound effect on PuPE, which was equally highest in T1 (0.26 kg total P uptake kg−1 P supply) and T2 (0.25) and lowest in T3 (0.16) and T4 (0.18). Similarly, PPFP, PHI and PPB followed a similar trend, with highest values in T1 (57 kg tuber dry matter yield kg−1 P supply, 76.4 kg tuber P uptake kg−1 total plant’s P uptake and 0.20 kg tuber P uptake kg−1 P supply, respectively). Among the tested legume intercrops, dolichos competed least for P with the main crop (potato) hence it can be integrated into potato-based cropping systems without compromising potato tuber yield.

Genotypic Variation in Cotton Genotypes for Phosphorus-Use Efficiency

Low phosphorus (P) availability is a major constraint for cotton production. Consequently, P-efficient genotypes can improve productivity under conditions where the higher application of P is not economical. This study was conducted to characterize cotton genotypes for P-use efficiency under various P concentrations (0, 10, 20, 40, 80, and 500 μM KH2PO4). The results showed large genotypic variation in five selected traits, such as root dry weight, shoot dry weight, photosynthetic activity, P-utilization efficiency, and P-uptake efficiency. Based on these five selected traits, the genotypes were grouped into three main classes as efficient, moderate efficient, and inefficient genotypes as proposed by different researchers. Most of the genotypes behaved in a similar pattern under different P concentrations. Among the genotypes, Xinluzao-49 and Xinluzao-48 were considered as P efficient while CCRI-64 and Yumian-21 as inefficient genotypes. However, the rest of the genotypes were considered as moderately P efficient. The results prove that a large genetic potential exists in cotton genotypes for P-use efficiency, and the use of P-efficient genotypes for cultivation will reduce the application of phosphatic fertilizers. Furthermore, the use of P-efficient genotypes will improve cotton breeding activities and help in improving the environmental sustainability of cotton production.

Eucalypts and low phosphorus availability: between responsiveness and efficiency

Twenty-four species of eucalypts were studied regarding their ability to grow under low P and their responsiveness to P inputs. Growth and photosynthesis-related parameters were evaluated. Growth of all species was influenced by low P availability. No significant correlation was found between leaf P concentration and biomass, indicating that P concentrations in leaves cannot be solely considered an indication of the responsiveness to P in eucalypts. Species responsive to P-input (high agronomic P efficiency values, APE) were those with low P use efficiency - PUE (here assessed as relative efficiency of P-use, REP) and low P uptake efficiency (PUpE). But, non-responsive species were related to higher P-efficiency under low soil P-availability. Eucalyptus tereticornis, E. cladocalyx, E. globulus and E. camaldulensis were efficient under low-P availability. Whereas, E. crebra and E acmenoides were the most responsive species, with high APE, suggesting that for these species P-inputs are needed to guarantee plant growth. The root:shoot ratio remained constant at different P availabilities, suggesting that biomass allocation towards the root in response to P and greater investment in roots were not correlated with greater PUE. Under limited P, E. robusta and E. botryoides exhibited low foliar P contents and higher root:shoot ratios than those of other species with higher P contents, indicating that greater root investment does not necessarily result in greater PUE. The results suggest that the divergence among species is probably related to different mechanisms, which may improve P-use efficiency.

The effect of pH on morphological and physiological root traits of Lupinus angustifolius treated with struvite as a recycled phosphorus source

AimsPhosphorus (P) recovery from specific waste streams is necessary to develop environmentally sustainable and efficient fertilizers, achieving maximum productivity with minimum losses. A promising example of a P-recovery product is struvite (MgNH4PO4⋅6H2O). Phosphorus availability from struvite is profoundly influenced by soil pH and/or processes in the rhizosphere. Root exudates (e.g., organic anions) and root morphology affect fertilizer bioavailability. The overall objective of our study was to identify root morphological and physiological traits of the narrow-leaf lupine (Lupinus angustifolius L. subsp. angustifolius, cultivar: blau “Boregine”) involved in the acquisition of P from struvite, compared with KH2PO4 as a soluble P source. The study included different pH conditions, as soil pH is one of the main factors affecting P availability.MethodsNarrow-leaf lupine plants were grown in river sand under three pH conditions (4.5, 6.5 and 7.5). Three different P treatment conditions were used: 1) KH2PO4 (KP); 2) MgNH4PO4⋅6H2O (Struvite), both supplied at 15 μg P g−1 dry sand; and 3) no P addition (Nil-P), as control. Organic acids in the rhizosheath were collected. Root morphological parameters such as specific root length and root diameter were analyzed.ResultsThere was no significant difference in total plant biomass detected under any pH condition between struvite and KP treatments. In both acidic and alkaline conditions, the P-uptake efficiency (PUE: mg P plant−1/cm2 root surface area) with struvite was significantly greater than with KP. At neutral pH, there was no difference in PUE between plants supplied with KP or struvite. Plants growing at neutral pH showed greater root exudation of carboxylates (mainly citrate) when struvite was added compared with KP. At alkaline pH, the exudation per unit root surface area was greater than that at acidic or neutral pH. Plants growing in acidic pH had a higher specific root length (SRL) compared with those grown at alkaline or neutral pH.ConclusionsSimilar P-uptake efficiency from struvite and KH2PO4 at neutral pH in conjunction with the higher total biomass compared to the Nil-P treatment (70% higher) suggests very effective mobilization of P from struvite by carboxylate exudation. Application of struvite, while taking into account the different strategies for nutrient mobilization, can increase the use efficiency of this recovered P source.

Responses to phosphorus among barley genotypes

Genetic improvement in phosphorus (P) use efficiency (i.e. the ratio of biomass or yield at nil P to that at a given rate of application) is an important goal to improve P recovery and P efficiency of farming systems. Experiments were conducted at three sites in South Australia between 2009 and 2011 to characterise genetic variation in yield with no applied P and in the response to P fertiliser among a diverse range of barley (Hordeum vulgare L.) genotypes. In each experiment, 39–54 genotypes were grown at 0 or 30 kg P/ha. Responses to P were measured near the beginning of stem elongation by using normalised difference vegetation index (NDVI) and by harvesting the grain. Rhizosheath size was also measured on seedlings. Consistent differences in growth and yield at 0 kg P/ha were measured among the genotypes. By contrast, there were large environmental effects on responses to P, but some genotypes showed consistent responses. Measurements of growth, yield and P uptake on a subset of genotypes showed that most of the variation in biomass and yield could be attributed to variation in P-uptake efficiency (net total P uptake per unit available P) rather than to P-utilisation efficiency (biomass or yield per unit total P uptake). The size of the rhizosheath made a small contribution to variation in NDVI but not grain yield, suggesting that rhizosheath size may be of some benefit to early growth but that this does not persist through to yield. Genetic correlations between NDVI and yield were often weak but were generally positive at 0 kg P/ha. Correlations between responses in NDVI and responses in grain yield were low and often negative. The study identified several barley genotypes that showed consistent differences in yield at low P and responses to P; however, selection for P efficiency based solely on responses in vegetative growth may not be appropriate. Variation in P uptake appeared to be more important than P-utilisation efficiency for P efficiency in barley.

Kin recognition in plants with distinct lifestyles: implications of biomass and nutrient niches

Kin recognition has been demonstrated by plant biomass allocation and morphology traits as well as by nitrogen (N) uptake, but has not been examined from a nutrient-niche view yet. In this study, four species with distinct lifestyles, including Glycine max (L.) Merr. (herbaceous legume), Belamcanda chinensis (L.) DC. (herbaceous non-legume), Caesalpinia pulcherrima (L.) Sw. (woody legume), and Populus tomentosa (L.) Carr. (woody non-legume) were used to demonstrate kin recognition by estimating their biomass and allocation, as well as nutrient niches based on their uptake efficiency for N, phosphorus (P), sulfur (S), potassium (K), calcium (Ca), magnesium (Mg), and iron (Fe). For G. max, kin recognition was achieved by increased biomass, and by reduced nutrient-uptake efficiency of N, P, S, K, Ca, Mg, and Fe (decreased nutrient niches) to decrease nutrient competition among kin plants compared to the strangers. Although B. chinensis and C. pulcherrima had no biomass response, kin plants of B. chinensis increased, whereas C. pulcherrima decreased their S-uptake efficiency compare to strangers. Therefore, kin competition occurred in B. chinensis through increased nutrient niche whereas kin recognition occurred in C. pulcherrima through decreased nutrient niche. By comparison, P. tomentosa showed the co-occurrence of kin recognition and competition by increased root allocation and decreased P-uptake efficiency. These findings suggest that the biomass allocation and plant nutrient niches based on their nutrient-uptake efficiency can be used as potential parameters to identify kin recognition.

Potato Response to Phosphorus Fertilization at Two Sites in Nova Scotia, Canada

Identifying phosphorus (P) requirements and P-use efficiency is crucial to a sustainable potato industry in Eastern Canada. In a three-year study (2013–2015) conducted in Nova Scotia, Canada, we assessed the potato (CV Superior) yield response to P fertilization on two different soils in the Annapolis Valley. Soil organic matter ranged from 3.0% to 4.1% and from 2.0% to 2.5% at the Kentville and Sheffield sites, respectively. Initial soil phosphorus was higher at Kentville site, ranging from 81 to 162 mg kg−1 than at Sheffield site ranging from 75 to 109 mg kg-1. A randomized complete block design was used with five P rates (0, 17.5, 35, 52, and 79 kg P ha−1) applied at planting. Tuber yields were assessed at harvest, and P-uptake efficiency characteristics were measured before vine senescence. Total and marketable yields were not impacted by P rates. Marketable yield was 68% and 57% greater for Kentville than for Sheffield in 2013 and 2015, respectively and were significantly affected by P rates × year interactions at a 5.4% probability level. Quadratic functions were used to describe tuber yield responses to P rates (0.61 > R2 < 0.85) and P rates corresponding to the maximum yields were 17.5 kg P ha−1 in 2013 and 2015 and 35 kg P ha−1 in 2014 when data from both sites were pooled together. Phosphorus uptake efficiency ranged from 0.47 to 0.54 g DM mg−1 P offtake at Kentville and from 0.45 to 0.49 g DM mg−1 P offtake at Sheffield and was 13% and 7% greater at Kentville than at Sheffield in 2013 and 2015, respectively. While further studies are still needed for recalibration, results from this study provide some of the first information regarding potato response to P fertilization in Nova Scotia. Based on current P recommendations in the region for the same soil P levels, our results suggest that current P rates can be reduced without impacting potato yield.

Phosphorus efficient potato cultivars for Nilgiris

Field experiments were conducted at Central Potato Research Station, Muthorai, the Nilgiris, Tamil Nadu forthree years with seven potato cultivars under four levels of phosphorus application to evaluate their phosphorususe efficiency. Cultivars Kufri Neelima and Kufri Swarna were found more P efficient because of their higherrelative biomass production, tuber yield efficiency index, harvest index, agronomic use efficiency (AUE) and Puptake efficiency. These two cultivars produced higher tuber yields under no P and at Pmax of standard variety. The cultivars Kufri Girdhari, Kufri Jyoti and Kufri Himalini proved P responsive as they responded well to itsapplication and produced very low yields under no P application. Higher root biomass in these two efficientcultivars could be the reason behind their higher P use efficiency in comparison to other cultivars. The two Pefficient potato cultivars also happened to be resistant to potato cyst nematode, which are very common andserious problem in Nilgiris. The mechanism to have resistance against PCN, whose cysts emerge only in thepresence of root exudates of susceptible potato cultivars, could also have benefitted those cultivars to showmore efficiency in native P utilization. Further investigations are required to find out the exact mechanism of Pefficiency in these two PCN resistant cultivars.

Overcoming Phosphorus Deficiency in West African Pearl Millet and Sorghum Production Systems: Promising Options for Crop Improvement.

West Africa (WA) is among the most food insecure regions. Rapid human population growth and stagnating crop yields greatly contribute to this fact. Poor soil fertility, especially low plant available phosphorus (P) is constraining food production in the region. P-fertilizer use in WA is among the lowest in the world due to inaccessibility and high prices, often unaffordable to resource-poor subsistence farmers. This article provides an overview of soil P-deficiency in WA and opportunities to overcome it by exploiting sorghum and pearl millet genetic diversity. The topic is examined from the perspectives of plant breeding, soil science, plant physiology, plant nutrition, and agronomy, thereby referring to recent results obtained in a joint interdisciplinary research project, and reported literature. Specific objectives are to summarize: (1) The global problem of P scarcity and how it will affect WA farmers; (2) Soil P dynamics in WA soils; (3) Plant responses to P deficiency; (4) Opportunities to breed for improved crop adaptation to P-limited conditions; (5) Challenges and trade-offs for improving sorghum and pearl millet adaptation to low-P conditions in WA; and (6) Systems approaches to address soil P-deficiency in WA. Sorghum and pearl millet in WA exhibit highly significant genetic variation for P-uptake efficiency, P-utilization efficiency, and grain yield under P-limited conditions indicating the possibility of breeding P-efficient varieties. Direct selection under P-limited conditions was more efficient than indirect selection under high-P conditions. Combining P-uptake and P-utilization efficiency is recommendable for WA to avoid further soil mining. Genomic regions responsible for P-uptake, P-utilization efficiency, and grain yield under low-P have been identified in WA sorghum and pearl millet, and marker-assisted selection could be possible once these genomic regions are validated. Developing P-efficient genotypes may not, however, be a sustainable solution in itself in the long-term without replenishing the P removed from the system in harvested produce. We therefore propose the use of integrated soil fertility management and systems-oriented management such as enhanced crop-tree-livestock integration in combination with P-use-efficiency-improved varieties. Recycling P from animal bones, human excreta and urine are also possible approaches toward a partially closed and efficient P cycle in WA.

P accumulation and physiological responses to different high P regimes in Polygonum hydropiper for understanding a P-phytoremediation strategy.

Phosphorus (P) accumulators used for phytoremediation vary in their potential to acquire P from different high P regimes. Growth and P accumulation in Polygonum hydropiper were both dependent on an increasing level of IHP (1–8 mM P) and on a prolonged growth period (3-9 weeks), and those of the mining ecotype (ME) were higher than the non-mining ecotype (NME). Biomass increments in root, stem, and leaf of both ecotypes were significantly greater in IHP relative to other organic P (Po) sources (G1P, AMP, ATP), but lower than those in inorganic P (Pi) treatment (KH2PO4). P accumulation in the ME exceeded the NME from different P regimes. The ME demonstrated higher root activity compared to the NME grown in various P sources. Acid phosphatase (Apase) and phytase activities in root extracts of both ecotypes grown in IHP were comparable to that in Pi, or even higher in IHP. Higher secreted Apase and phytase activities were detected in the ME treated with different P sources relative to the NME. Therefore, the ME demonstrates higher P-uptake efficiency and it is a potential material for phytoextraction from P contaminated areas, irrespective of Pi or Po contamination.

Comparison of Phosphorus Use Efficiency Among Various Winter Wheat Accessions Grown in Acid and Calcareous Soils

The objective was to differentiate and estimate phosphorus (P)-uptake and P-utilization efficiency among several winter wheat accessions. Twenty-two accessions were characterized for their ability to uptake and utilize P in two low P soils (acid and calcareous). Plants were grown for 28 days in two different sized pots and analyzed for biomass and total P concentration. Total P uptake and biomass was greater in the large pot and calcareous soil. However, there were no significant correlations between the small and large pot for biomass, P uptake, and P concentration, indicating that rooting conditions were different for the two pots, which allowed differentiation between P-utilization and P-uptake efficiency. Possession of ALTM1 gene for malate excretion did not appear to be related to P-uptake efficiency. Several accessions were found to be either P-uptake or P-utilization efficient in both soils. Phosphorus use efficiency mechanisms may contribute to acid soil tolerance.

Role of arbuscular mycorrhizal symbiosis in phosphorus-uptake efficiency and aluminium tolerance in barley growing in acid soils

Arbuscular mycorrhizal fungi (AMF) play an important role in protecting plant growth against such stresses as phytotoxic aluminium (Al) in soil. To understand some of the AMF interactions that relate to amelioration of Al phytotoxicity and phosphorus (P)-uptake efficiency in barley (Hordeum vulgare L.), this study examined the effect of soil Al levels and mycorrhizal symbiosis on plant response, including root colonisation, AMF propagules and glomalin production. A greenhouse experiment was conducted using two native barley cultivars, Sebastián and Aurora, grown in an acidic soil at two Al-saturation levels (80% Al-sat, unlimed soil; 7% Al-sat, limed soil) with and without AMF propagules. Root dry weight, total and colonised root lengths, and root P and Al contents were determined at 60 and 150 days after sowing. AMF spore density, total hyphal length, glomalin-related soil protein (GRSP) and Al bound to GRSP (Al-GRSP) were analysed at final harvest. AM root colonisation was not inhibited in limed soil, mycorrhizal propagule numbers increased at high Al levels, and Al-GRSP ranged from 5.6% to 8.3% of the total GRSP weight. These values also increased in unlimed soil, particularly those associated with cv. Aurora. Root Al concentration correlated inversely with AMF spores (r = –0.85, P &lt; 0.001) and Al-GRSP (r = –0.72, P &lt; 0.01), but only in plants growing in limed soil. Conversely, the AMF treatments in which Al was present showed a greater relationship between total root length and both root Al (r = –0.72, P &lt; 0.01) and root P (r = 0.66, P &lt; 0.01) concentrations. Sebastián showed a greater response to lime, whereas Aurora responded better to mycorrhizal presence. The relative growth rate of roots, P uptake efficiency and mycorrhizal parameters such as root colonisation, spores, hyphae and GRSP showed Aurora to be more Al-tolerant than Sebastián. In conclusion, the greater rate of increase of AM propagules, GRSP and Al-GRSP associated with cv. Aurora supports the hypothesis that AMF play an important role in the Al-tolerance capacity and P-uptake efficiency of H. vulgare growing in soils with high Al levels.

Effect of Phosphorus on the leaf yield, nutrient uptake and P-use efficiencies of sun cured chewing tobacco (Nicotiana tobacum) grown in Tamil Nadu

A field experiment was conducted during 2005-2009 to study the effect of various P management practices on the productivity, chewing quality, nutrient uptake, P-use efficiencies on chewing tobacco. There is a dire need to reduce the P requirement for chewing tobacco (Nicotiana tabacum L.), as it is costlier and scarce. First grade leaf yield (FGLY) and total cured leaf yield (TCLY) with 50,75 and 100% recommended P with or without phospho bacteria (PSB) are comparable. The FGLY and TCLY increase varied between 7 to 20 % and 19 to 30 % respectively over no P. Phosphorus application irrespective of the levels improved the chewability (Score &gt;60) over no P. The uptake of NPK and soil available P improved with levels of P with or without PSB over no P. Phosphorus application every year improved the soil available P. The P balance showed loss of P in all the treatments. P-use efficiency (39.5- 42.3 kg/kg), P-uptake efficiency (0.12 - 0.15 kg/kg) , agronomic efficiency (0.12 - 0.15 kg/kg) , recovery efficiency (7.27 7.55%) and partial factor productivity (138 - 148 kg/kg) was higher at 50% P with or without PSB. Physiological efficiency was higher at 100% P +PSB (315 kg/ha) in the first year and 50% P (395.3 kg/ha) in the second year. Net returns and B:C ratio with 100% P was comparable with 75 and 50 % P with or without PSB. It was concluded that 50% recommended P (22 kg P/ha) would be sufficient for higher yield , net returns, better leaf chemistry and P-use efficiency.

Modelling root plasticity and response of narrow-leafed lupin to heterogeneous phosphorus supply

Searching for root traits underpinning efficient nutrient acquisition has received increased attention in modern breeding programs aimed at improved crop productivity. Root models provide an opportunity to investigate root-soil interactions through representing the relationships between rooting traits and the non-uniform supply of soil resources. This study used simulation modelling to predict and identify phenotypic plasticity, root growth responses and phosphorus (P) use efficiency of contrasting Lupinus angustifolius genotypes to localised soil P in a glasshouse. Two L. angustifolius genotypes with contrasting root systems were grown in cylindrical columns containing uniform soil with three P treatments (nil and 20 mg P kg−1 either top-dressed or banded) in the glasshouse. Computer simulations were carried out with root architecture model ROOTMAP which was parameterized with root architectural data from an earlier published hydroponic phenotyping study. The experimental and simulated results showed that plants supplied with banded P had the largest root system and the greatest P-uptake efficiency. The P addition significantly stimulated root branching in the topsoil, whereas plants with nil P had relatively deeper roots. Genotype-dependent root growth plasticity in response to P supply was shown, with the greatest response to banded P. Both experimental and simulation outcomes demonstrated that 1) root hairs and root proliferation increased plant P acquisition and were more beneficial in the localised P fertilisation scenario, 2) placing P deeper in the soil might be a more effective fertilisation method with greater P uptake than top dressing, and 3) the combination of P foraging strategies (including root architecture, root hairs and root growth plasticity) is important for efficient P acquisition from a localised source of fertiliser P.