Ozark Research Articles

Mapping landforms of a hilly landscape using machine learning and high-resolution LiDAR topographic data

Landform maps are important tools in assessment of soil- and eco-hydrogeomorphic processes and hazards, hydrological modeling, and natural resources and land management. Traditional techniques of mapping landforms based on field surveys or from aerial photographs can be time and labor intensive, highlighting the importance of remote sensing products based automatic or semi-automatic approaches. In addition, the time-intensive manual labeling can also be subjective rather than an objective identification of the landform. Here we implemented such an objective approach applying a random forest machine learning algorithm to a set of observed landform data and 1m horizontal resolution bare-earth digital elevation model (DEM) developed from airborne light detection and ranging (LiDAR) data to rapidly map various landforms of a hilly landscape. The landform classification includes upland plateaus, ridges, convex slopes, planar slopes, concave slopes, stream channels, and valley bottoms, across a 400 km2 hilly landscape of the Ozark Mountains in northeastern Oklahoma. We used 4200 landform observations (600 per landform) and eight topographic indices derived from 2 m, 5 m and 10 m resolution LiDAR DEM in random forest algorithm to develop 2 m, 5 m and 10 m resolution landform models. We test the effectiveness of DEM resolution in mapping landforms via comparison of observed landforms with modeled landforms. Results showed that the approach mapped ∼84% of observed landforms when covariates were at 2 m resolution to ∼89% when they were at 10 m resolution. However, predicted maps showed that the 2 m resolution covariates performed better at capturing accurate landform boundaries and details of small-sized landforms such as stream channels and ridges. The approach presented here significantly reduces the time required for mapping landforms compared to traditional techniques using aerial imagery and field observations and allows incorporation of a wide variety of covariates. The landform map developed using this approach has several potential applications. It could be utilized in hydrological modeling, natural resource management, and characterizing soil-geomorphic processes and hazards in a hilly landscape.

Ornithophily in the trumpet creeper (Campsis radicans).

The diversification of hummingbirds (Trochilidae) has shaped the pollination strategies and floral trait evolution in at least 68 families of flowering plants in the Western Hemisphere. The trumpet creeper (Bignoniaceae) is the quintessential example of ornithophily in eastern North America. The mutualistic relationship between this orange-flowered liana and the ruby-throated hummingbird (Archilochus colubris) was illustrated as early as 1731. However, neither historical nor modern accounts accurately describe the feeding behavior of ruby-throats at trumpet creeper flowers or the floral adaptations for ornithophily. This paper explores their surprisingly immersive mode of foraging at trumpet creeper flowers and quantitatively assesses floral traits in two populations in the Ozark Mountains. The ruby-throat's bill is approximately one-third the length of the trumpet-shaped flowers, which counters the tendency for the corolla length of ornithophilous plants to match the bill length of their principal hummingbird pollinator. To access the nectary, ruby-throats grasp or cling to the ventral petal lobe of the corolla with their claws and thrust their head and upper body into the flower. This immersive "floral-diving" had not been formally documented among the 356 species of hummingbirds until now. The didynamous anthers and stigma are strategically positioned inside the corolla to brush the crown feathers when the ruby-throat inserts its head. A narrow stricture in the corolla, about a third of the way up, allows the bill and tongue of hummingbirds to pass while blocking bumblebees and carpenter bees from reaching the nectary. As a result, the abundant sucrose-rich floral nectar seems to be reserved for hummingbird pollinators.

Identifying and Prioritizing Factors to Improve Implementation of an Evidence-Based Program for Child Maltreatment Prevention in Rural Missouri Communities

AbstractPrevention of child abuse and maltreatment is critical in the Ozark region of Missouri where rates are higher than in other areas of the state. Community-based organizations seeking to address these issues in rural areas face significant challenges both internal and external to the organization. Guided by the Consolidated Framework for Implementation Research (CFIR), Whole Kids Outreach (WKO) utilized a modified concept mapping approach to identify strategies needed to address these challenges and more effectively implement the Healthy Families America (HFA) program in seven counties in the rural Missouri Ozark region. Semi-structured interviews were used to identify barriers and supports to the implementation of HFA. Statements were generated via in-person and telephone interviews with key stakeholders (WKO internal staff and external partners). These stakeholders then sorted statements into like piles and rated them according to importance. Concept mapping systems software, using multidimensional scaling and hierarchical cluster analysis, was used to determine the most salient cluster solution. The resulting cluster map depicted six thematic clusters and provided a visual representation of relationships between clusters. Stakeholders were then guided through a nominal group process to prioritize key issues and begin to develop strategies to address them.

A Diffusion‐Based Uncertainty Quantification Method to Advance E3SM Land Model Calibration

AbstractCalibrating land surface models and accurately quantifying their uncertainty are crucial for improving the reliability of simulations of complex environmental processes. This, in turn, advances our predictive understanding of ecosystems and supports climate‐resilient decision‐making. Traditional calibration methods, however, face challenges of high computational costs and difficulties in accurately quantifying parameter uncertainties. To address these issues, we develop a diffusion‐based uncertainty quantification (DBUQ) method. Unlike conventional generative diffusion methods, which are computationally expensive and memory‐intensive, DBUQ innovates by formulating a parameterized generative model and approximates this model through supervised learning, which enables quick generation of parameter posterior samples to quantify its uncertainty. DBUQ is effective, efficient, and general‐purpose, making it suitable for site‐specific ecosystem model calibration and broadly applicable for parameter uncertainty quantification across various earth system models. In this study, we applied DBUQ to calibrate the Energy Exascale Earth System Model land model at the Missouri Ozark AmeriFlux forest site. Results indicated that DBUQ produced accurate parameter posterior distributions similar to those from Markov Chain Monte Carlo sampling but with 30 times less computing time. This significant improvement in efficiency suggests that DBUQ can enable rapid, site‐level model calibration at a global scale, enhancing our predictive understanding of climate impacts on terrestrial ecosystems.

Catastrophic storms, forest disturbance, and the natural history of Swainson's warbler.

The core breeding range of Swainson's warbler (Limnothlypis swainsonii) overlaps a zone of exceptionally high tornado frequency in southeastern North America. The importance of tornadoes in creating breeding habitat for this globally rare warbler and other disturbance-dependent species has been largely overlooked. This paper estimates tornado frequency (1950-2021) and forest disturbance in the 240 counties and parishes in which breeding was documented from 1988 to 2014. The frequency of destructive tornadoes (EF1-EF5) varied 6-fold across the breeding range with a peak in the Gulf Coast states. Counties from east Texas to Alabama experienced the lowest median return interval of 5.4 years per 1000 km2, resulting in approximately 2477 ha of forest damage per 1000 km2 per century, based on current forestland cover. Tornadoes were significantly less frequent north and east of the core breeding range, with return intervals increasing to 9.1 years per 1000 km2 for breeding counties on the Atlantic coastal plain, 10.2 years per 1000 km2 in the Ozark Mountains, and 32.3 years per 1000 km2 in the Appalachian Mountains. Breeding counties within 150 km of the coastline from east Texas to North Carolina are also subjected to the highest frequency of hurricanes in the Western Hemisphere. Hurricanes often cause massive forest damage but archived meteorological and forestry data are insufficient to estimate the aggregate extent of forest disturbance in breeding counties. Nevertheless, the combined impact of tornadoes and hurricanes in the pre-Anthropogenic era was likely sufficient to produce a dynamic mosaic of early-successional forest crucial for the breeding ecology of Swainson's warbler. To ensure the long-term survival of this rare warbler, it is advisable to develop habitat management plans that incorporate remote sensing data on early-successional forest generated by catastrophic storms as well as anthropogenic activities.

Putting eDNA to the test: A field comparison of eDNA metabarcoding to established protocols for assessing biodiversity in Missouri's Ozark Highland streams

AbstractAquatic biodiversity monitoring to inform conservation and management efforts in‐stream systems has increasingly begun to incorporate environmental DNA (eDNA)‐based sampling methods. We conducted a comparison of eDNA metabarcoding to a traditional protocol of combined seining and electrofishing methods to assess fish biodiversity of wadeable stream sites in six separate drainages in the Ozark Highlands of Missouri (USA). The study further focused on the headwaters of the Meramec River, which included 11 sites and seasonal sampling (summer and winter). We compared estimates of diversity across sampling methods, assessed the influences of water flow (depth, velocity, and discharge) and season, and tested the effects of sampling method and site locality on fish assemblage composition. eDNA sampling detected approximately double the number of species compared to traditional methods, providing higher diversity estimates while maintaining the relative ranking of sites. eDNA detection probabilities were positively associated with stream depth and velocity and were generally higher in summer than in winter but not for all species. Estimated species richness was positively associated with discharge for both methods but the relationship was stronger with eDNA sampling. Assemblage differences between tributary and mainstem sites were attributable to a small number of species that were found predominantly in one stream size category or the other, indicating that eDNA was sensitive to within‐drainage assemblage structure relationships. We highlight improved species detection, a more comprehensive understanding of assemblage structural dynamics, and the potential ability to integrate data across sampling methods as important benefits that encourage the use of eDNA metabarcoding as a primary collection method in future stream biodiversity assessment and monitoring programs.

Germplasm of Ozark chinquapin (Castanea ozarkensis Ashe) can be cryopreserved by dormant winter buds

Ozark chinquapin (Castanea ozarkensis Ashe) is a forest tree, endemic to the Ozark Mountain region in Eastern United States. Its nutritious nuts were consumed by Native Americans, European settlers, livestock, and wild animals and its wood was an important rot-resistant construction material. Once a significant tree in regional forest communities, the species was nearly eradicated by a chestnut blight caused by Cryphonectria parasitca (Murill) Barr fungus. Some individuals have survived as sprouts from adventitious root buds, but they rarely reach reproductive maturity. While some in situ restoration efforts are underway, the development of a viable ex situ germplasm preservation method is critical to the conservation of this important food-bearing species. Our experiment aimed to develop a cryopreservation method for C. ozarkensis dormant winter buds subjected to eight experimental treatments before desiccation, slow cooling, and storage in liquid nitrogen vapor. The highest post cryogenic viability was 91.2 % for dormant buds pretreated with 0.3 M sucrose for 16 h followed by 0.75 M sucrose for 3 h; this treatment is suggested for cryopreservation of dormant winter buds of Ozark chinquapin germplasm.

Ways parental health and stress shape the parenting of preteens in the rural Ozark Mountains

AbstractObjectiveWe explore how parental health and stress shape the rearing of preteen children in the rural Ozark Mountains.BackgroundIdentifying concerns that impact preteen parents' health and stress levels may facilitate understanding ways to promote resilience and happiness for families in underserved rural communities.MethodsUsing an interpretive phenomenological approach grounded in Lazarus's stress and coping paradigm, the current qualitative study used semistructured interviews with 20 White parents in the rural area of the Ozark Mountains who had at least one child born between 2008–2011.ResultsStudy participants' stories revealed that stressors related to parenting preteen children were rooted in stress from mental health or indirectly tied to social roles, such as occupational stress or financial constraints.ConclusionInterventions and tools need to be developed that are specific to the culture of rural areas and that focus on families as an essential part of developing resilience and happiness and mitigating the adverse effects of stress.ImplicationsInterventions focused on families need to include diverse social factors that impact family resilience and happiness and include education to increase health literacy, family supports related to improving connections between parents and their preteen children, family tax credits to reduce hardships for parents, a tool for screening parents for stress, and expansion of telehealth opportunities.

Microaggressions in the heartland “flyover” region: history, progress, lessons learned and challenges

PurposeThis article sheds light on the impact of collective characteristics of microaggression in a community and how this affects the perception and experiences of its underrepresented members through the lens of critical race theory (CRT). Using the Springfield community of the southwest Missouri Ozarks region in the United States of America as the authors' focus, the authors explore the barrier of microaggression in the lived experiences of a community striving for diversity and inclusion.Design/methodology/approachThe authors performed a systematic analysis using four CRT tenets: race is a social construct; racism is systematic, racism is commonplace and listening to lived experiences is essential. A sample of underrepresented professionals from the region was surveyed to obtain their lived experiences. Qualitative media analysis on varied media pieces was conducted to obtain context for the environment that precipitated these experiences.FindingsWhen residents from marginalized backgrounds face consistent microaggressions, their sense of belonging and contributions to the community significantly diminish, which has adverse implications for the community. If these issues are unaddressed, they might choose to leave due to feelings of underrepresentation. Community leaders must proactively implement strategies to welcome an evolving population and educate stakeholders about the detrimental effects of microaggressions on community cohesion.Originality/valueHistorically, the heartland of the United States of America has been a bellwether of the pulse of its average citizen. This region is currently experiencing an increase in diversity along with a significant rise in persistent microaggressions. Using CRT to analyze the impact, lessons learned and challenges, the authors provide recommendations for potential changes that could benefit the nation as a whole.

Novel Ozark Orthohantavirus in Hispid Cotton Rats (Sigmodon hispidus), Arkansas, USA.

We report a novel orthohantavirus, putatively named Ozark orthohantavirus, in hispid cotton rats captured within the Ozark Plateau in Arkansas, USA. This virus phylogenetically clusters with other orthohantaviruses that cause severe human disease. Continued orthohantavirus surveillance and virus sequencing are needed to address the potential public health threat of this virus.

Survey of southern Missouri Plethodontidae in Ozark caves

Plethodontidae is a large family of salamanders with a distribution ranging from eastern North Americato northern South America. Three genera and nine species occur in Missouri, most of which are typically found in cavesfor at least part of their lives. These species have been recorded to emerge from hibernation in early April and throughoutMay. This study shares the species abundance and diversity in ten Missouri Ozark caves: seven near St. Louis andthree near Springfield. Each cave was surveyed at least once between November 2021 and September 2022 using visualencounter surveys. Observational data were recorded without handling any individuals to minimize disturbance. Thethree caves near St. Louis had few to no salamanders present in early April, despite optimal weather conditions, buttended to have larger populations throughout the summer. Two caves near Springfield held at least a dozen individualsof multiple species in mid-April. Few individuals were recorded in the most southwestern cave sampled, potentiallydue to pollution or recent flooding. Our findings could aid others studying plethodontid salamanders to determine theprime conditions for emergence from hibernation, optimal habitats for different species in each cave zone, and potentialbiological or chemical impacts that could affect salamander abundance in caves.

Apparent annual survival of female eastern small-footed bats (Myotis leibii) roosting in Arkansas bridges

Abstract The Eastern Small-footed Bat (Myotis leibii) inhabits mountainous areas of the eastern United States and generally roosts in cliff faces and talus slopes. However, the difficulty of accessing these sites has limited research on this species. We studied survival rates of eastern small-footed bats by capturing bats roosting in crevices between sections of concrete guardrails on bridges in the Ozark mountains of northern Arkansas from 2014 to 2021. We accumulated 1,413 bat captures (1,050F; 363M) of 283 individual females and 198 individual males sampled over 8 years and determined apparent annual survival of female bats using Cormack–Jolly–Seber survival models. Only 1 of 30 bats tested positive for the presence of Pseduogymnoascus destructans, a fungus causing white-nose syndrome (WNS) which is responsible for significant declines in populations of some North American bat species, when all maternity colonies were sampled in the spring of 2019. Overall apparent annual survival of all females was 0.643, with first-year juvenile survival of 0.472 and adult (ages 1–6) survival of 0.744, approximately 58% greater than first-year survival. Apparent annual survival did not differ greatly among the sites or among years. We found no significant decline or increase in overall number of bats among all sites during the 8 years of study. Stability in both the number of bats encountered and their survival rates suggests that the impact of WNS on these colonies has been marginal.

Survival and movements of brown‐headed nuthatches after translocation to the Missouri Ozarks

AbstractThe brown‐headed nuthatch (Sitta pusilla) was likely extirpated from Missouri in the early 1900s as a result of habitat loss through extensive logging. Conservation partners including the Missouri Department of Conservation, United States Forest Service, University of Missouri, and others, relocated 102 brown‐headed nuthatches from Ouachita National Forest in Arkansas to Mark Twain National Forest in Missouri in 2020 and 2021 to establish a local population. We tracked 50 individuals for 24 ± 11 (median ± SD) days after release using radio telemetry and monitored movements in relation to sex and whether a bird was captured alone or as part of a group. We examined 25‐day survival using a spatial Cormack Jolly Seber (sCJS) model to account for likely dispersal outside of our study area. All birds survived capture, transport, and release. The 25‐day survival estimate was 0.56 (95% CI = 0.36–0.78) in 2020 and 0.68 (95% CI = 0.46–0.89) in 2021. Mean total distance moved following release was 5,670 (SD = 3,407) m and mean daily movement was 243 (SD = 135) m which was less than the mean daily dispersal distance estimated from our sCJS model (420 m, SD = 221). Our short‐term monitoring suggests initial success of the translocation, but continued monitoring is needed to evaluate long‐term success. Our survival analysis approach, which included movement data, illustrates the importance of considering dispersal when estimating survival.

Spatial acclimation of elk during population restoration to the Missouri Ozarks, USA

AbstractWildlife translocations are an important conservation tool but can be challenging for the animals. For translocations to be successful, animals must adjust to their release landscape. Investigating how animals acclimate to their release landscapes can improve post‐release monitoring and inform about the management needs of translocated populations. We investigated movements and resource selection dynamics of 106 elk (Cervus canadensis) during the first 6–8 years following their release to Missouri, USA in 2011–2013. We observed evidence of spatial acclimation as determined by cessation of changes in resource selection together with monthly range sizes and fidelity of individual elk. Females showed faster evidence of acclimation in their movements following release than males. Although range fidelity for both sexes stabilized within approximately seven months, range size stabilized within approximately three months for females and stabilization began within approximately 11 months for males. Selection for multiple resources by elk also largely stabilized within their first year following release. A simple refuge‐forage trade‐off alone did not explain acclimation in resource selection. Elk selected for high‐quality food plots across the temporal extent of restoration regardless of time since release, whereas their selection for cover resources during summer increased after elk acclimated. Together, spatial acclimation generally lagged behind physiological responses observed in this system, adding to the increasing evidence that translocated animals display acclimation patterns across trait‐specific time periods. Our approach demonstrates the utility of accounting for acclimation effects across multiple spatial response metrics for improving post‐release monitoring, evaluation, and management of restored wildlife populations.

Late Quaternary–Recent intraplate topographic uplift of the St. Francois Mountains (Ozark Plateau), Missouri (USA), driven by differential density and erosion

AbstractThe origin of relatively high topography in intraplate settings is elusive. The St. Francois Mountains, a tectonically inactive region with high relief, a Mesoproterozoic granite and rhyolite core, and Paleozoic sedimentary flanks, provide insights into the evolution of intraplate landscapes. We determined 14 in situ10Be exposure ages, 23 10Be catchment-wide denudation rates (CWDRs), and geomorphic indices using a geographic information system, and we modeled surface uplift using an isostatic-flexure model. CWDRs varied from ~5 to 10 ± 1.7 mm/k.y. and averaged 1.5 times greater in streams draining granite and rhyolite than in those draining flanking sedimentary rocks. In situ results from bedrock strath terraces gave Quaternary incision ages and incision rates that were tenfold higher than CWDRs. Major knickpoints (locally called shut-ins), all at 200 ± 70 m elevation, are located at contacts between the igneous and sedimentary rocks. Our exposure and denudation results inform isostatic-flexural modeling, which together support the conclusion that late Quaternary–Recent surface uplift driven by differential density and erosion focused in the igneous core is creating significant topography and relief in this intraplate setting.

A multi-method approach for assessing the distribution of a rare, burrowing North American crayfish species.

Primary burrowing crayfishes face high extinction risk, but are challenging to study, manage, and conserve due to their difficult-to-sample habitat (i.e., terrestrial burrows) and low population densities. We apply here a variety of methods to characterize the distribution, habitat associations, and conservation status of the Boston Mountains Crayfish Cambarus causeyi (Reimer, 1966), an endemic burrowing crayfish found only in the Ozark Mountains of Arkansas, United States. We used species distribution modeling (SDM) on historic occurrence records to characterize the distribution and macro-scale habitat associations of this species. We then ground-truthed SDM predictions with conventional sampling, modeled fine-scale habitat associations with generalized linear models (GLM), and lastly developed and tested an environmental DNA (eDNA) assay for this species in comparison to conventional sampling. This represents, to our knowledge, the first successful eDNA assay for a terrestrial burrowing crayfish. Our MaxEnt-derived SDM found a strong effect of average annual precipitation on the historic distribution of C. causeyi, which occurred most frequently at locations with moderately high average annual precipitation (140-150 cm/yr) within our study region. Cambarus causeyi was difficult to detect by conventional sampling in 2019 and 2020, found at only 9 of 51 sites (17.6%) sampled by searching for and manually excavating crayfish burrows. Surprisingly, habitat suitability predicted from our MaxEnt models was not associated with contemporary C. causeyi occurrences per GLMs. Instead, C. causeyi presence was negatively associated with both sandy soils and the presence of other burrowing crayfish species. Poor SDM performance in this instance was likely caused by the omission of high resolution fine-scale habitat data (e.g., soils) and biotic interactions from MaxEnt models. Finally, our eDNA assay detected C. causeyi from six of 25 sites (24.0%) sampled in 2020, out-performing conventional surveys by burrow excavation for this species. Given the difficulty of studying primary burrowing crayfishes and their high conservation need, we propose that eDNA may become an increasingly important monitoring tool for C. causeyi and similar species.

Anthropogenic channel network development in forested watersheds by recent stream channelization practices in Mark Twain National Forest, Missouri Ozarks

Human modifications of stream systems often result in channel instability and sediment pulse- induced problems. While previous research explored the direct anthropogenic impacts of channelization on fluvial systems, the history of repeated channelization, their persistent impacts on headwater streams and subsequent evolution of a drainage system need further investigation. This study evaluates the spatial distribution of channel form and stability in Big Barren Creek watershed, which drains the karst dominated Ozark Highlands of southeast Missouri. The objectives of this research are threefold: (i) describe channel morphology and hydrology in undisturbed and disturbed segments based on field data, (ii) propose a conceptual model of channel modification and response to explain the patterns of channel adjustment and (iii) evaluate the history of channel disturbance using historical aerial photograph analysis. Results show that disturbed reaches have larger channel dimensions than undisturbed reaches because channelization increased stream power and shear stress, particularly for channels that are actively incising. Channelized reaches are primarily identified on private land where headcuts developed and migrated upstream to the national forestland. Sediment supply to the channel increases from both upstream incision and bank and levee collapse resulting in downstream aggradation, which forces landowners to repeat channelization for flood control. The proposed conceptual model suggests that while the disturbed segments evolve into wider and deeper channels following channelization, they exhibit the potential to become stable over time because of biogeomorphic resistance (e.g., in-channel vegetation, armored-cohesive soil) related to the ephemeral nature of the streams in this karst region. This idea is also supported by the slower average headcut migration rate of earlier channelization (2 m/yr) compared to recent channelization (6–8 m/yr). However, channel adjustment largely depends on repeated channelization that resets the evolutionary stages that preclude this fluvial system from attaining stability.

Home in the hills: rural stayers in the Missouri Ozarks and Nebraska Sandhills

ABSTRACT Drawing on results from online surveys of the mobility of people who grew up in Missouri (2021) and Nebraska (2019), this article situates itself among emerging research on immobile rural populations. By using an immobility prospective, this research draws attention to the most influential factors in an individual’s active, intended, and deliberate decision to stay in two of the most rural regions of the United States: the Missouri Ozarks and the Nebraska Sandhills. The findings of both the Missouri and Nebraska Roots Migration Surveys suggest that deep familial roots and a sense of rurality among those who grow up in rural America bond individuals with their home places and influence their residential decision-making.

Aquatic Macroinvertebrate Community Structure along a Continuum in a Spring Dominated River, Missouri, USA

The Current River is a cold-water, thermally constant Missouri Ozark stream and is one of the few remaining free-flowing rivers in the U.S. The Current River’s baseflow is largely fed by hundreds of springs, which include five first-magnitude springs. Little attention has been given to the influence of spring inflows on river temperature and spring influence on biodiversity. The objectives of this study were to demonstrate how large springs affect river macroinvertebrate communities, and to explore the relationships among macroinvertebrate diversity and habitat variables to estimate spring influences on community structure and diversity. Aquatic macroinvertebrates were collected from 42 riffle/run habitats of the Current River main-stem, tributaries, and springs during the winter season. Samples at each site were collected using a Slack-Surber sampler for macroinvertebrates with additional habitat variables collected: substrate size, embeddedness, periphyton, filamentous green algae, vegetation, depth, current velocity, temperature, dissolved oxygen, specific conductance, and pH. Beta diversity analysis was performed on consecutive pairs of site taxa richness values using the Wilson–Shmida calculation to determine the impact of main-stem confluences with either tributaries or springs, and invertebrate community relationships were explored using nonmetric multidimensional scaling (NMDS). Water temperature and taxa richness exhibited similar patterns, with higher temperatures being associated with lower taxa richness. Downstream of each large-magnitude spring, taxa richness sharply decreased, while taxa richness increased downstream of tributaries. Beta diversity usually declined downstream of the confluences with springs, but increased downstream of the tributaries. Data from large springs were closely grouped in NMDS, while tributaries and main-stem sites were more widely scattered. These data indicate spring inputs produce more homogenous conditions in the main-stem river compared to more heterogenous conditions produced by tributary inputs. Macroinvertebrate diversity along the Current River also does not follow predictions from the river continuum concept, but rather diversity peaks are downstream of springs. Our data clearly demonstrate the strong influence of large springs on macroinvertebrate communities in the Current River.

Roost Characteristics of a Tricolored Bat Perimyotis subflavus in the Missouri Ozarks

The tricolored bat (Perimyotis subflavus) once commonly occurred throughout the eastern and central United States, but is now experiencing range-wide population declines primarily due to white-nose syndrome and forest fragmentation. Conservation efforts for this species require more information regarding summer maternity roost characteristics, especially in the Ozark Highlands of Missouri, where the species has declined precipitously. Herein we report the capture and tracking of one female tricolored bat to two summer roosts in southeastern Missouri. Tree roosts differed by their substrates, as one was within a cluster of dead leaves on a snag and the other was located under exfoliating bark; otherwise, the roosts and surrounding vegetation were similar. Both roosts were located in large (>30 cm diameter breast height) oak (Quercus spp.) trees under a dense canopy of trees with similar basal areas. While our capture success was limited over two years, details regarding tricolored bat roost ecology are imperative for the species' conservation and management.