Oxygen Depletion Rates Research Articles

High thermodynamical sensitivity of CO2 emissions from a large oligotrophic-hardwater lake (Nam Co) on the Tibetan Plateau

The Tibetan Plateau (TP) has the world's largest distribution of high-alpine and saline (generally hardwater) lakes, which are expected to affect regional carbon cycling profoundly. However, the variability, and especially underlying factors controlling CO2 dynamics, across widespread hardwater lakes is poorly understood on the TP. Here, we present year-round records of surface water pCO2 from a representative hardwater lake (Nam Co) on the TP, and analyze relationships between ambient variables and pCO2 during open water (i.e., ice-free) and ice-covered months. Surface pCO2 (233.3 μatm on average) was a little oversaturated to atmosphere (219 μatm on average) during the open water season. As a CO2 source, Nam Co emitted 8.73 ± 1.06 Gg C annually, but this flux only accounted for 0.53 ± 0.06 ‰ of its total dissolved inorganic carbon pool (1.64 × 1013 g C). Regression results indicate that, during open water months, both seasonal and diurnal varying patterns of surface pCO2 were influenced predominantly by water temperature, in a quasi-marine mode, by controlling gas solubility and dissolved carbonate equilibria. Therefore, CO2 evasion was elevated during summer months, despite the lake being autotrophic (i.e., CO2 consumption via photosynthesis). By contrast, during ice-covered months the surface pCO2 was strongly related to under-ice thermodynamics, and declined nonlinear with increased inversed stratification. In the hypolimnion, as a result of extremely weak metabolism (as indicated by low dissolved oxygen depletion rates) and a combined high carbonate buffering effect, accumulation of CO2 was negligible, leading to an absence of peak effluxes of CO2 during turnover periods, compared to eutrophic freshwater lakes. We argue that, under future global warming scenarios, consideration of the impact of gradually warming lake water on thermodynamics and dissolved carbonate equilibria are vital in order to understand the future CO2 dynamics of these widespread high-altitude oligotrophic-hardwater lakes situated across the TP.

A polyphenol-rich cranberry supplement improves muscle oxidative capacity in healthy adults.

Cranberries are rich in polyphenols, have a high antioxidant capacity, and may protect against exercise-induced free radical production. Mitochondria are known producers of free radical in skeletal muscle, and preventing overproduction of radicals may be a viable approach to improve muscle health. This study aimed to investigate the effect of a polyphenol-rich cranberry extract (CE) on muscle oxidative capacity and oxygenation metrics in healthy active adults. 17 participants (9 males and 8 females) were tested at: (i) baseline, (ii) 2 h following an acute CE dose (0.7g/kg of body mass), and (iii) after 4 weeks of daily supplement consumption (0.3g/kg of body mass). At each time point, muscle oxidative capacity was determined using near-infrared spectroscopy to measure the recovery kinetics of muscle oxygen consumption following a 15-20s contraction of the vastus lateralis. Cranberry supplementation over 28 days significantly improved muscle oxidative capacity (k-constant, 2.8±1.8vs. 3.9±2.2; p=0.02). This was supported by a greater rate of oxygen depletion during a sustained cuff occlusion (-0.04±0.02vs. -0.07±0.03; p=0.02). Resting muscle oxygen consumption was not affected by cranberry consumption. Our results suggest that cranberry supplementation may play a role in improving mitochondrial health, which could lead to better muscle oxidative capacity in healthy active adult populations.

Oxygen Enhancement Ratio-Weighted Dose Quantitatively Describes Acute Skin Toxicity Variations in Mice After Pencil Beam Scanning Proton FLASH Irradiation With Changing Doses and Time Structures

PurposeTo investigate the ability of a biological oxygen enhancement ratio weighted dose, DOER, to describe acute skin toxicity variations observed in mice after proton pencil beam scanning (PBS) irradiations with changing doses and beam time structures. Materials and methodsIn five independent experiments, the right hind leg of a total of 621 CDF1 mice was previously irradiated in the entrance plateau of a PBS proton beam. The incidence of acute skin toxicity (of level 1.5-2.0-2.5-3.0-3.5) was scored for 47 different mouse groups that mapped toxicity as function of dose for conventional and FLASH dose rate, toxicity as function of field dose rate with and without repainting, and toxicity when splitting the treatment into 1-6 identical deliveries separated by 2 minutes. DOER was calculated for all mouse groups using a simple oxygen kinetics model to describe oxygen depletion. The three independent model parameters (oxygen depletion rate, oxygen recovery rate, oxygen level without irradiation) were fitted to the experimental data. The ability of DOER to describe the toxicity variations across all experiments was investigated by comparing DOER-response curves across the five independent experiments. ResultsAfter conversion from the independent variable tested in each experiment to DOER, all five experiments had similar MDDOER50 (DOER giving 50% toxicity incidence) with standard deviations of 0.45-1.6 Gy for the five toxicity levels. DOER could thus describe the observed toxicity variations across all experiments. ConclusionDOER described the varying FLASH sparing effect observed for a wide range of conditions. Calculation of DOER for other irradiation conditions can quantitatively estimate the FLASH sparing effect for arbitrary irradiations for the investigated murine model. With appropriate fitting parameters DOER may also be able to describe FLASH effect variations with dose and dose rate for other assays and endpoints.

Oxygen depletion and sediment respiration in ice‐covered arctic lakes

AbstractProcesses regulating the rate of oxygen depletion determine whether hypoxia occurs and the extent to which greenhouse gases accumulate in seasonally ice‐covered lakes. Here, we investigate the oxygen budget of four arctic lakes using high‐frequency data during two winters in three shallow lakes (9–13 m maximal depth) and four winters in 24 m deep main basin of Toolik Lake. Incubation experiments measured sediment metabolism. Volume‐averaged oxygen depletion measured in situ was independent of water temperature and duration of the ice‐covered period. Average rates were between 0.2 and 0.39 g O2 m−2 d−1 in the shallow lakes and between 0.03 and 0.14 g O2 m−2 d−1 in Toolik Lake, with higher rates in smaller lakes with their larger sediment area to volume ratio. Rates decreased to ~ 20%–50% of initial values in late winter in the shallow lakes but less or not at all in Toolik. The lack of a decline in Toolik Lake points to continued oxygen transport to the sediment–water interface where oxygen consumption occurs. In all lakes, lower in situ oxygen depletion than in incubation measurements points toward increasing anoxia in the lower water column depressing loss rates. In Toolik, oxygen loss during early winter was less in years with minimal snow cover. Penetrative convection occurred, which could mix downwards oxygen produced by photosynthesis or excluded during ice formation. Estimates of these terms exceeded photosynthesis measured in sediment incubations. Modeling under ice‐oxygen dynamics requires consideration of optical properties and biological and transport processes that modify oxygen concentrations and distributions.

Improving oxygen conditions in periodically stagnant basins using sea-based measures - Illustrated by hypothetical applications to the By Fjord, Sweden

To improve the water quality of a habitat or to stop eutrophication driven by internal loading of phosphorus from anoxic bottoms, the deepwater in inshore and offshore sea basins may be oxygenated using sea-based measures. The minimum oxygen concentration DOmin in periodically stagnant basins is controlled by three factors, namely (i) the rate of oxygen depletion dDO/dt. (ii) The residence time Te of the stagnant water. (iii) The oxygen concentration DOstart in the basin water at the beginning of a stagnation period. A theoretical framework is presented, suitable for evaluation of sea-based measures to improve the oxygen conditions in periodically stagnant basins. Five different sea-based measures to improve the oxygen conditions are recognized and discussed. The application of sea-based measures might be particularly attractive for habitat improvements in basins with poor oxygen conditions despite land-based measures are fully implemented. The sea-based measures are illustrated by hypothetical applications to the By Fjord on the west coast of Sweden, a salt-stratified fjord with periodically stagnant anoxic basin water confined behind the shallow Sunninge Strait in the mouth. An interesting result of one of the hypothetical applications is that dredging of the Sunninge Strait in 1958 and 1976 reduced tidal currents by a factor of 4. This reduced the tidal power supply to vertical mixing in the basin water, which led to prolonged residence time Te, from 2-3 years to 3–5 years, and deteriorated oxygen conditions in the basin water.

Seasonal evolution and controlling factors of bottom oxygen depletion in the Bohai Sea

A coupled physical-biogeochemical model is used to investigate the seasonal evolution and controlling factors of oxygen depletion in the Bohai Sea (BS). Comparisons show that the model reproduces observed spatiotemporal variations of important physical and biogeochemical variables well. Bottom oxygen in the BS shows an annual cycle with significant drawdown in summer and enhanced replenishment in fall. Two oxygen-depleted regions off Qinhuangdao (QHD) and the Yellow River estuary (YRE) develop separately and experience higher oxygen depletion rates and longer durations of low-oxygen conditions. The evolution of oxygen depletion is primarily controlled by stratification and biological oxygen consumption but is also modulated by lateral transport. Strong stratification is established earlier than oxygen depletion and maintains its development. The biological oxygen consumption determines the two oxygen-depleted regions under stratified conditions. Lateral transport influenced by anticyclonic circulations favors an expansion of oxygen depletion off QHD but alleviates oxygen depletion off the YRE.

Drivers of fish biodiversity in a rapidly changing permafrost landscape

Abstract Rapid environmental change occurring in northern permafrost regions may have profound implications for fish biodiversity but remains poorly understood. Climate change, increasing human development, and resultant permafrost thaw may combine to alter the quality and quantity of fish habitat including reductions in preferred thermal habitat, changes in water quality, and modified drainage patterns. Our study objective was to understand how lake fish communities residing on permafrost landscapes may be responding to climate change and land use disturbance. We investigated the drivers of freshwater fish community health in lakes of the lower Mackenzie River basin, an ice‐rich permafrost region that is experiencing substantial warming, permafrost thaw, and new major highway development. We collected lake morphometry, water quality, and fish community data from 50 lakes and derived several indicators of aquatic health including fish species richness, relative abundance, and the occurrence of three culturally important fish species. We found that water quality and lake size were significant co‐drivers of fish community health whereas relationships with summer thermal habitat, as represented by July air temperature, were relatively negligible. Dissolved organic carbon (an indicator for lake browning) emerged as a particularly important driver of fish community structure, and fish community health steeply declined when dissolved organic carbon concentrations exceeded 17–18 mg/L. We suggest potential mechanisms for these declines including light inhibition during summer and a reduced capacity for overwintering in smaller and murkier lakes that may experience faster oxygen depletion rates. Using a more expansive regional water quality database of 203 lakes, we observed potential supporting evidence that warming and new road development increased dissolved organic carbon and total phosphorus concentrations, possibly reducing fish habitat quality in this region. Together, these results highlight how fishes relying on the numerous small and shallow lakes that dominate permafrost landscapes may be vulnerable to the combined effects of rapid warming and new infrastructure.

Kinetics of silicon nitride coatings degradation and its influence on liquid infiltration in PV silicon crystallization processes

Safe implementation of reusable crucibles in the silicon PV industry requires a thorough understanding of the reactions in the system to avoid liquid infiltration and crucible failure at high temperatures. Typically, an oxidized Si 3 N 4 -coating layer is applied to the crucibles to avoid wetting. During melting, the coating undergoes a transition in its wettability behavior as a result of oxide depletion. However, much uncertainty still exists about undergoing reactions and their effect on the coating depletion, and hence the wetting kinetics. Here we report on the coating's oxygen depletion mechanisms by applying a novel coating method to avoid conventional oxidation as it causes severe degradation of non-oxide crucibles. By adding colloidal silica to the silicon nitride coating, we (i) control the oxygen concentration in the coating, (ii) avoid the crucible degradation, and (iii) eliminate the pre-oxidation step. Furthermore, a quantification of the coating's oxygen content effect on the depletion rate was presented via an analytical model. These results provided insight into how the coating depletion can hinder the liquid infiltration by forming and stabilizing silicon oxynitride in the coating as evidenced by Raman mapping and thermodynamic calculations. The difference between the rates of liquid infiltration and oxygen depletion was also elucidated in detail. • Developing a new coating method for non-oxide crucible materials. • Optimization of the oxygen content that is required to avoid wetting. • Modelling of the depletion rate of the coating at the three major regions. • Revealing the mechanism of infiltration and the major compounds that are responsible for non-wetting.

Increasing Carbon-to-Phosphorus Ratio (C:P) from Seston as a Prime Indicator for the Initiation of Lake Reoligotrophication.

Decline in total phosphorus (TP) during lake reoligotrophication does not apparently immediately influence carbon assimilation or deep-water oxygen levels. Traditional monitoring and interpretation do not typically consider the amount of organic carbon exported from the productive zone into the hypolimnion as a measure of net ecosystem production. This research investigated the carbon-to-phosphorus ratios of suspended particles in the epilimnion, (C:P)epi, as indicators of changing productivity. We report sestonic C:P ratios, phytoplankton biomass, and hypolimnetic oxygen depletion rates in Lake Hallwil, a lake whose recovery from eutrophic conditions has been documented in 35 years of historic water-monitoring data. This study also interpreted long-term (C:P)epi ratios from reoligotrophication occurring in four other lakes. Lake Hallwil exhibited three distinct phases. (i) The (C:P)epi ratio remained low when TP concentrations did not limit production. (ii) (C:P)epi increased steadily when phytoplankton began optimizing the declining P and biomass remained stable. (iii) Below a critical TP threshold of ∼15 to ∼20 mg P m-3, (C:P)epi remained high and the biomass eventually declined. This analysis showed that the (C:P)epi ratio indicates the reduction of productivity prior to classic indicators such as deep-water oxygen depletion.

Winter Oxygen Regimes in Clear and Turbid Shallow Lakes

AbstractDissolved oxygen controls important processes in lakes, from chemical reactions to organism community structure and metabolism. In shallow lakes, small volumes allow for large fluctuations in dissolved oxygen concentrations, and the oxygen regime can greatly affect ecosystem‐scale processes. We used high frequency dissolved oxygen measurements to examine differences in oxygen regimes between two alternative stable states that occur in shallow lakes. We compared annual oxygen regimes in four macrophyte‐dominated, clear state lakes to four phytoplankton‐dominated, turbid state lakes by quantifying oxygen concentrations, anoxia frequency, and measures of whole‐lake metabolism. Oxygen regimes were not significantly different between lake states throughout the year except for during the winter under‐ice period. During winter, clear lakes had less oxygen, higher frequency of anoxic periods, and higher oxygen depletion rates. Winter oxygen depletion rates correlated positively with peak summer macrophyte biomass. Due to lower levels of oxygen, clear shallow lakes may experience anoxia more often and for longer duration during the winter, increasing the likelihood of fish winterkills. These observations have important implications for shallow lake management, which typically focuses efforts on maintaining the clearwater state.

Assessing the Nutrient Dynamics in a Himalayan Warm Monomictic Lake

Thermal stratification is considered to be the most important limnological feature of deep lake ecosystems affecting a water column’s chemical characteristics thereby directly connected with lake management. The lake is also attracting the attention of management authorities, being a growing tourist destination with sustainable environmental management plan. Lake Manasbal is only true warm monomictic urban lake in Kashmir and remains thermally stratified for 8–9 months, with separate layers of epilimnion, metalimnion and hypolimnion.We noticed that in the lower part of the hypolimnion, the process of stratification is characterized by an oxygen deficit (O2 < 1 mg/L). The rate of oxygen depletion reported in the lake’s bottom layers is indicative of the water body’s more eutrophic nature. A clinograde type of pH curve with a wide range of fluctuation from top to bottom layers of water was observed. Thermal stratification of the lake allows ammonium and phosphates to pool in the hypolimnion for much of the stratification period, resulting in concentrations higher than in the epilimnion. Consequently, the lake was characterized by a nutrient-enriched hypolimnion and an epilimnion deprived of nutrients. With the advent of the lake stratification, the thermocline was formed on 15th March between 2 and 3 m and on 7th April between 4 and 6 m, which gradually moved down to 10 m on 4th July and remained at that depth until circulation. The concentration of phosphorus and nitrogen in Lake Manasbal showed an increase towards the bottom, thus revealing an inverse clinograde depth distribution. Due to stratification phenomena in this lake, the internal release and subsequent distribution of nutrients throughout water column especially phosphorus from sediment water interface after mixing is a cause of concern for management authorities as it has the potential to diminish the ecological and aesthetics of the lake.Therefore, we argue that this study will provide some valuable insights on how stratification is affecting the nutrient dynamics and will offer inputs for better management accordingly both from ecological and economic perspectives.

Environmental and climatic factors affecting winter hypoxia in a freshwater lake: evidence for a hypoxia refuge and for re-oxygenation prior to spring ice loss

Low dissolved oxygen, or hypoxia, is a common phenomenon in ice-covered lakes in winter. We measured dissolved oxygen (DO) before, during, and after ice-over to characterize the timing, severity, and spatial variability of winter hypoxia in Upper Red Rock Lake, Montana, home to one of the last remaining lacustrine populations of endemic Montana Arctic Grayling (Thymallus arcticus). Unlike most previous investigations of winterkill-prone lakes, we observed considerable horizontal spatial variability in DO, a non-linear winter oxygen depletion rate, and lake-wide re-oxygenation 2–4 weeks prior to spring ice loss. Parts of the upper 1 m of the lake and near stream mouths remained well-oxygenated even during late winter. DO levels were strongly associated with maximum daily air temperature. Our analysis of a 28-year weather record revealed large interannual variability in risk of winter hypoxia, with a slight declining trend in winter severity (number of days with maximum air temperatures ≤ 0°C) in Upper Red Rock Lake. The approach we used in our study provides a useful framework for quantifying and mapping the seasonal dynamics of the extent and severity of winter hypoxia, and for identifying critical winter habitats.

Hypolimnetic oxygen depletion rates in deep lakes: Effects of trophic state and organic matter accumulation

AbstractThis study investigated the consumption of oxygen (O2) in 11 European lakes ranging from 48 m to 372 m deep. In lakes less than ~ 100 m deep, the main pathways for O2 consumption were organic matter (OM) mineralization at the sediment surface and oxidation of reduced compounds diffusing up from the sediment. In deeper lakes, mineralization of OM transported through the water column to the sediment represented a greater proportion of O2 consumption. This process predominated in the most productive lakes but declined with decreasing total phosphorous (TP) concentrations and hence primary production, when TP concentrations fell below a threshold value of ~ 10 mg P m−3. Oxygen uptake by the sediment and the flux of reduced compounds from the sediment in these deep lakes were 7.9–10.6 and 0.6–3.6 mmol m−2 d−1, respectively. These parameters did not depend on the lake's trophic state but did depend on sedimentation rates for the primarily allochthonous or already degraded OM. These results indicate that in lakes deeper than ~ 100 m, mineralization of autochthonous OM is mostly complete by the time of sedimentary burial. This explains why hypolimnetic O2 concentrations improve more rapidly following TP load reduction in deeper lakes relative to shallower lakes, where larger sediment‐based O2 consumption by settled OM and release of reduced substances may inhibit the restoration of hypolimnetic O2 concentrations.

Rates of oxygen-depletion in granular Lambeth Group strata and evidence for barometric pumping

Laboratory experiments have been performed to demonstrate significant levels of oxygen depletion within samples of Upnor Formation of the Lambeth Group deposits. These have been used to explain the reasons for several incidences of confined space hypoxia during underground construction within the stratum beneath London. Further investigation, using a relatively small-scale field pump-out test, revealed the rapid effects of dewatering on the generation of hypoxic gas (within the ground) and that the amount of oxygen falls to fatal levels within a very short time after commencement of pumping. Monitoring of boreholes during ground investigation for the Thames Tideway Tunnel has indicated barometric control on the release of hypoxic gas from installations within granular deposits of the Lambeth Group, namely the Upnor Formation, as well as channel sand deposits within the Laminated Beds. In these, reduced levels of oxygen coincide with low and/or falling barometric pressure. Continuous 24-hour monitoring demonstrates that the reduction is almost instantaneous and has serious implications for the Health and Safety of underground construction personnel.

Water column changes under ice during different winters in a mid-latitude Mediterranean high mountain lake

Winter limnology has been developed mainly in northern-temperate or subarctic lakes, while mid-latitude Mediterranean high mountain lakes have been less studied during the ice-covered season. We present a five-year study in one of these lower latitude high mountain lakes, conducted with multiprobes located at two different depths recording high-frequency data. We have studied highly contrasted winters regarding temperature and snowfall. The results of a principal component analysis and a subsequent hierarchical clustering show that bottom oxygen depletion under ice is strongly influenced by winter cover thickness and transparency. A thick and opaque winter cover (dark mode) leads to an intense oxygen depletion until anoxia, while a thin and clear winter cover (clear mode) allows higher dissolved oxygen saturation due to oxygen production by photosynthesis. Under unusual conditions of cold and dry climate, only a weak decrease in bottom dissolved oxygen was observed. The extent of dissolved oxygen depletion and its relationship with meteorological parameters and climate patterns are further investigated with generalized additive models. Winter cover thickness and phenology are driven by the joint effect of the North Atlantic Oscillation and East Atlantic climatic patterns. Ice phenology could also influence the following ice-free period given the correlation of lake heat content with the ice-off date and the ice cover length. We propose some features jointly occurring in Lake Cimera as a model for mid-latitude Mediterranean high mountain lakes, including (yet not exclusive) the occurrence of very thick winter covers, the prevalence of dark mode, the relatively high and similar through the years oxygen depletion rates, and high water temperatures during the ice-free periods. It is unclear how the ongoing climate change will affect the prevalence of dark over clear modes due to the expected antagonistic effects of warmer temperatures and lower snowfalls.

Impact of Target Oxygenation on the Chemical Track Evolution of Ion and Electron Radiation.

The radiosensitivity of biological systems is strongly affected by the system oxygenation. On the nanoscopic scale and molecular level, this effect is considered to be strongly related to the indirect damage of radiation. Even though particle track radiolysis has been the object of several studies, still little is known about the nanoscopic impact of target oxygenation on the radical yields. Here we present an extension of the chemical module of the Monte Carlo particle track structure code TRAX, taking into account the presence of dissolved molecular oxygen in the target material. The impact of the target oxygenation level on the chemical track evolution and the yields of all the relevant chemical species are studied in water under different irradiation conditions: different linear energy transfer (LET) values, different oxygenation levels, and different particle types. Especially for low LET radiation, a large production of two highly toxic species ( and ), which is not produced in anoxic conditions, is predicted and quantified in oxygenated solutions. The remarkable correlation between the and production yield and the oxygen enhancement ratio observed in biological systems suggests a direct or indirect involvement of and in the oxygen sensitization effect. The results are in agreement with available experimental data and previous computational approaches. An analysis of the oxygen depletion rate in different radiation conditions is also reported. The radiosensitivity of biological systems is strongly affected by the system oxygenation. On the nanoscopic scale and molecular level, this effect is considered to be strongly related to the indirect damage of radiation. Even though particle track radiolysis has been the object of several studies, still little is known about the nanoscopic impact of target oxygenation on the radical yields. Here we present an extension of the chemical module of the Monte Carlo particle track structure code TRAX, taking into account the presence of dissolved molecular oxygen in the target material. The impact of the target oxygenation level on the chemical track evolution and the yields of all the relevant chemical species are studied in water under different irradiation conditions: different linear energy transfer (LET) values, different oxygenation levels, and different particle types. Especially for low LET radiation, a large production of two highly toxic species ( and ), which is not produced in anoxic conditions, is predicted and quantified in oxygenated solutions. The remarkable correlation between the and production yield and the oxygen enhancement ratio observed in biological systems suggests a direct or indirect involvement of and in the oxygen sensitization effect. The results are in agreement with available experimental data and previous computational approaches. An analysis of the oxygen depletion rate in different radiation conditions is also reported.

Modeling the Kinetics, Curing Depth, and Efficacy of Radical-Mediated Photopolymerization: The Role of Oxygen Inhibition, Viscosity, and Dynamic Light Intensity.

Kinetic equations for a modeling system with type-I radical-mediated and type-II oxygen-mediated pathways are derived and numerically solved for the photopolymerization efficacy and curing depth, under the quasi-steady state assumption, and bimolecular termination. We show that photopolymerization efficacy is an increasing function of photosensitizer (PS) concentration (C0) and the light dose at transient state, but it is a decreasing function of the light intensity, scaled by [C0/I0]0.5 at steady state. The curing (or cross-link) depth is an increasing function of C0 and light dose (time × intensity), but it is a decreasing function of the oxygen concentration, viscosity effect, and oxygen external supply rate. Higher intensity results in a faster depletion of PS and oxygen. For optically thick polymers (>100 um), light intensity is an increasing function of time due to PS depletion, which cannot be neglected. With oxygen inhibition effect, the efficacy temporal profile has an induction time defined by the oxygen depletion rate. Efficacy is also an increasing function of the effective rate constant, K = k′/, defined by the radical producing rate (k′) and the bimolecular termination rate (kT). In conclusion, the curing depth has a non-linear dependence on the PS concentration, light intensity, and dose and a decreasing function of the oxygen inhibition effect. Efficacy is scaled by [C0/I0]0.5 at steady state. Analytic formulas for the efficacy and curing depth are derived, for the first time, and utilized to analyze the measured pillar height in microfabrication. Finally, various strategies for improved efficacy and curing depth are discussed.

An innovative method to calculate oxygen consumption rate

Based on heat and mass transfer characteristics of spontaneous combustion of coal, Arrhenius equation and the Ranz-Marshall correlation, a novel approach was proposed in this paper to estimate oxygen consumption rate of self-ignition of coal at high temperature. Compared with the conventional methods, this approach involves not only kinetic properties of self-ignition of coal and temperature, but also the ambient air flow characteristics and diameter of coal particle. To testify the proposed approach, oxygen consumption rates at high temperature were measured by the programmable isothermal oven experiments. Comparisons between experimental and theoretical results indicate that the rates of oxygen depletion calculated by the proposed approach agree well with those measured from laboratory-scale experiments, which further validates the proposed approach.

Oxygen Management at the Microscale: A Functional Biochip Material with Long-Lasting and Tunable Oxygen Scavenging Properties for Cell Culture Applications.

Oxygen plays a pivotal role in cellular homeostasis, and its partial pressure determines cellular function and fate. Consequently, the ability to control oxygen tension is a critical parameter for recreating physiologically relevant in vitro culture conditions for mammalian cells and microorganisms. Despite its importance, most microdevices and organ-on-a-chip systems to date overlook oxygen gradient parameters because controlling oxygen often requires bulky and expensive external instrumental setups. To overcome this limitation, we have adapted an off-stoichiometric thiol-ene-epoxy polymer to efficiently remove dissolved oxygen to below 1 hPa and also integrated this modified polymer into a functional biochip material. The relevance of using an oxygen scavenging material in microfluidics is that it makes it feasible to readily control oxygen depletion rates inside the biochip by simply changing the surface-to-volume aspect ratio of the microfluidic channel network as well as by changing the temperature and curing times during the fabrication process.

In situ, high-resolution time series of dissolved phosphate in Green Bay, Lake Michigan

Abstract In nearly every instance in which the environment has been sampled on a higher resolution in time or space, fundamental processes have come to light that were previously undetected or unobserved. In this study, an autonomous dissolved phosphate sensor was deployed at the Entrance Light station in lower Green Bay, Lake Michigan in 2012 and 2013. Hourly phosphate sensor measurements were compared with other real time sensor data to gain insights into the processes occurring at this site. Results showed that the water column at this location undergoes repeated stratification and turnover during the course of the summer. Often, the stratification results from intrusions of cold hypolimnetic bottom water from the north, while turnover is associated with significant northerly and/or easterly wind events. It was observed that, during calm periods, dissolved phosphate concentrations increased at a rate that was stoichiometrically consistent with the consumption of dissolved oxygen during the remineralization of organic matter; specifically, areal oxygen consumption rates ranged from 3.2 to 43 mmol m−2 d−1 and oxygen to phosphate ratios ranged from 120 to 210. At other times, the inverse relationship between dissolved oxygen and dissolved phosphate was not stoichiometrically linked; during these times, areal oxygen depletion rates ranged from 51 to 240 mmol m−2 d−1 and oxygen to phosphate ratios ranged from 260 to 2300. Future strategic deployment of multiple in situ dissolved phosphate and other nutrient sensors will enhance the understanding of nutrient cycling in this important aquatic system.