Oxide In Blood Serum Research Articles

The Correlation Between the Degree of Pain with Estradiol Hormone Levels, Interleukin 1β (il-1β) Levels and Nitrite Oxide in Blood Serum of Endometriosis Patients

Endometriosis is the chronic disease, in which the glandular and stromal-like endometrium grows outside the uterine cavity, Endometriosis is inflammatory and estrogen-dependent that affects 6-10% of women during their reproductive years and up to 50% of women receiving fertility treatment. The purpose of this study was to determine the correlation between the degree of pain with estradiol hormone levels, interleukin-1β (IL-1β) levels and Nitrite Oxide (NO) in the blood serum of patients with endometriosis. This type of research is an observational analytic with a cross sectional comparative study design in which the dependent and independent variables are obtained simultaneously. The research was conducted in type B and C hospitals. To take blood serum samples for endometriosis patients and the Biomedical Laboratory of FK Unand to check levels of estradiol hormone, levels of Interleukin-1β (IL-1β) and NO. The study was conducted in January - November 2020. The population was all patients who doing examination at the obstetrics gynecology department of the hospital. Samples were taken consecutively that met the inclusion and exclusion criteria with a total sample of 40 people. Data analysis using analyzed by univariate, bivariate and multivariate with chi-square test. The research results were obtained The mean age of endometriosis patients was 38.85 years, the mean pain degree of patients and the distribution of levels of estradiol, interleukin, 1β (IL-1β) and Nitric oxide. The results of the bivariate analysis showed that there were differences degree of pain with each Estradiol level, interleukin, 1β (IL-1β) as well as nitric oxide. The results of the multivariate analysis showed that there was a difference in the degree of pain with the three Estradiol levels. interleukin, 1β (IL-1β) and nitric oxide in the blood serum of people with endometriosis (P = 0,000 then p [α (0.05). From the research results it can be concluded that there are the relationship between degree of pain and level Estradiol, interleukin, 1β (IL-1β) and nitric oxide in the blood serum of people with endometriosis.

The role of catecholamines, melatonin and nitric oxide in the mechanisms of stress damage to the body

The mechanisms of response and adaptation of the body’s regulatory systems to stress factors is an urgent fundamental task and the goal of this study. The study used four experimental models of stress: acute stress, chronic stress, and their sequences. An acute stress response was induced in a 60-minute forced swimming test model in a pool. Chronic stress was simulated by restricting mobility (hypokinesia), which was done by placing the rats into special AE1001-R1 rat retainers, made of transparent polypropylene (OOO OpenScience, Russia), in which they were kept for 10 days, 20 hours a day. The levels of biogenic amines (adrenaline, norepinephrine, serotonin), melatonin and nitric oxide in blood serum were determined by a method of enzyme-linked immunosorbent. the results obtained provide fair evidence of an increased functional activity of both stress-response (sympathoadrenal system) and stress-limiting systems (ST, MT, NO) in stress reactions of the body, the severity of which depends on the duration and sequence of stress stimuli on animals.

The study of flow-mediated vasodilation of the brachial artery and other markers of endothelial function in children with familial hypercholesterolemia

Objective. To study the state of endothelial function in children with familial hypercholesterolemia by evaluating the flow-dependent vasodilation of the brachial artery and serum nitric oxide and endothelin-1 levels. Children characteristics and research methods . The study included total 62 children: 30 children (mean age 10,3 years ± 2,8) with a heterozygous form of familial hypercholesterolemia, not taking lipid-lowering therapy (main group), and 32 healthy children (mean age 10,3 years ± 2,8) in the control group. The content of endothelin-1 and nitric oxide in blood serum were determined by enzyme immunoassay. Flow-dependent vasodilation of brachial artery was measured using Philips Affinity 70 (Release 3,0.3, Philips Healthcare, USA). Results. The analysis of endothelin-1 level did not reveal statistically significant differences between healthy children and patients with familial hypercholesterolemia (0,213 [0,012; 0,368] and 0,227 [0,15; 0,315] pmol/l accordingly; p=0,062). The level of nitrogen oxide in healthy children was 33,6 [20—51] pmol/l, while patients with familial hypercholesterolemia had statistically significantly higher level of nitrogen oxide — 103,7 [48—152] pmol/l, p=0,001). Flow-dependent brachial artery vasodilation in healthy children was 9,82 [7,8; 11,2]% and in patients with familial hypercholesterolemia this indicator was decreased — 9,46 [6,5; 10,8]% (p=0,742). Conclusion. A study of endothelial function in children with familial hypercholesterolemia aged 7—11 years revealed an increase in the concentration of nitrogen oxide relative to healthy patients, probably of a compensatory nature. The flow-dependent vasodilation of the brachial artery and the level of endothelin-1 did not significantly differ from the control group. Due to possible cumulative effect of low-density proteins, it is necessary to monitor endothelial function in children with familial hypercholesterolemia in dynamics.

МЕТОД ПРОГНОЗИРОВАНИЯ РАЗВИТИЯ ВЫСОКОЙ СТЕПЕНИ ВЫРАЖЕННОСТИ ВОСПАЛИТЕЛЬНОГО ПРОЦЕССА У ДЕТЕЙ С ОСТРЫМИ ВНЕГОСПИТАЛЬНЫМИ ПНЕВМОНИЯМИ

Aim of study. To identify a set of independent factors and develop a method for predicting the high degree of severity of the inflammatory process in children with acute community-acquired pneumonia. Material and methods. A cohort of 100 patients was examined, including children with acute pneumonia with (n=70) and without (n=30) endothelial dysfunction. All children underwent a comprehensive study, which included a clinical examination, laboratory studies according to generally accepted methods. The study of the function of external respiration was carried out using the spirograph “Spiro-spectrum”. Determination of the content of nitric oxide in blood serum was performed by spectrophotometric method using cadmium and Griss reagent, circulating endothelial cells by microscopy. Results. A regression model was created that enables to predict the probability of a high degree of inflammatory process in children with segmental and lobar pneumonia (sensitivity 96.4%, specificity 93.3%). Conclusions. The use of the regression equation enabled to develop a method for predicting the probability of a high degree of inflammatory process in children with segmental and lobar pneumonia that have signs of endothelial dysfunction, and as a result – to choose the optimal management strategy for such patients.

Prognosing a severe course of asthma in children following the study of endothelial function

Abstract Background Asthma is a common disease with increasing prevalence in children and adults. The WHO estimates that annually 15 million disability-adjusted life-years are lost, and 250,000 asthma deaths are reported worldwide. Approximately, 500,000 annual hospitalizations are due to asthma (1). Aim In our study, we aimed to evaluate the endothelial function in children with asthma in remission and the prognosis of severe asthma. Materials and methods The study involved examination of 91 children, aged 6–17 years, with persistent asthma in the remission period. Indices of endothelial function (soluble vascular molecule of intercellular adhesion-1 [sVCAM-1], concentration of stable metabolites of nitric oxide in blood serum [NO2 +NO3 ], thickness of the intima-media complex [IMC] of the common carotid artery (CCA), and endothelium-dependent dilatation of the brachial artery [FMD%]). Statistical analyses were performed with StatSoft STATISTICA Version 8 (Tulsa, OK). To determine the relation between qualitative characteristics, the criterion χ2 was used, and the procedure of multiple logistic regression analysis was performed. Results The endothelium parameter levels (FMD% [H = 46.02], IMC [H = 60.75], NO2 + NO3 [H = 40.82], and sVCAM-1 [H = 76.57, p = 0.0000]) depend on the severity of the disease. The study showed that the factors that should be taken into account in prognosis of the formation of the severe course of asthma include positive family allergic history, serum sVCAM-1 and NO2 + NO3 levels, and the thickness of IMC CCA. Conclusions All the children with asthma in the remission period were found to have endothelial dysfunction. The degree of disruption of the function of the endothelium depends on the severity of the course of asthma. An algorithm for predicting the severe course of asthma in children has been developed.

THE EVALUATION OF NOISE EFFECT ON BIOCHEMICAL INDICES OF ENDOTHELIAL DYSFUNCTION IN WORKERS OF SELWYNITE ORE-DRESSING AND PROCESSING ENTERPRISE

The labor conditions of workers of key professions of selwynite concentrating manufacture (drying machine operative, granulation machine operative, dosing of drying-granulating room machine operative, centrifuge operative, filtration operative, transporter of drying granulating room, engineer of mill machine) are characterized by effect of physical factors of industrial environment. The noise is the most significant of them. It is established that level of noise at working places of workers of analyzed specialties attains 83-98 dBA that on 18 dBA is higher of maximum permissible level of 80 dBA. In examined workers a reliable increasing of level of homocysteine and lipoprotein (a) up to 3.8 times is established against the background of decreasing of production of nitric oxide in blood serum up to 1.2 times and increasing of level of malonic di-aldehyde up to1.3 times relatively to similar indices in workers of comparison group. The consequence of these processes can be a disorder of structure and endothelial function of vessels, increasing of permeability of endothelium, loss of its elasticity and as a consequence disorder of functional activity of vessel wall. The substantiation is given to indicator indexes (level of lipoprotein (a), homocysteine in blood serum and content of malonic di-aldehyde in blood plasma), having higher and average degree of occupational conditionality and forming biochemical profile of endothelial dysfunction as a predictor of arterial hypertension in workers of selwynite ore-dressing and processing enterprise undergoing effect of noise. The given biochemical indices are appropriate to be applied in early diagnostic and prevention of endothelial dysfunction in workers of the mentioned enterprises.

Protective action of turmeric in alcoholic hepatitis and myelosupression

Изучен эффект масляного экстракта куркумы при алкогольном повреждении печени и миелосупрессии, индуцированной циклофосфаном у мышей. Установлено миело- и гепатопротекторное действие экстракта куркумы. Показано, что экстракт куркумы способствует сохранности лейкоцитов и спленоцитов после введения цитостатика. При алкогольном повреждении печени экстракт куркумы уменьшает повреждение тканей печени, что проявляется более низким уровнем в сыворотке крови и гомогенате печени трансаминаз, щелочной фосфатазы, билирубина и оксида азота.

Antioxidant status in albino rats vaccinated against salmonellosis under chronic action of T-2 toxin and its correction by aminoseleton

The article represents the study of the effect of chronic intoxication with T-2 toxin on the antioxidant status of albino rats under their vaccination against salmonellosis. It is stated that the intensity of lipid peroxidation (LPO) processes increased under the action of toxin and led to increased content of toxic product, malonic dialdehyde (MDA), in blood and decreased indices of antioxidant protection (AOP), activity of glutathione peroxidase (GPO) and catalase enzymes and level of vitamins E and A, in liver and nitric oxide in blood serum. The application of aminoseleton promoted the lower level of LPO, decreased MDA in blood, and increased potential of enzymatic and nonenzymatic components of AOP.

Inhibitory effect and mechanism of procyanidin from vaccinium on isoprenaline-induced myocardial fibrosis in rats

To detect the effect and mechanism of procyanidin foom vaccinium (PC) on myocardial fibrosis in rats. The myocardial fibrosis model in rats was built by subcutaneous injection of 5 mg x kg(-1) x d(-1) of isoprenaline (Iso) for 7 days in vivo while intragastrically administrating PC 100, 200 and 400 mg x kg(-1) x d(-1) for 14 days. Following the model was established, myocardial indexes (heart weight/body weight, HW/BW and left ventricalar weight/body weight, LVW/BW) were measured. Besides, angiotensin II and I , III collagen quantification levels in blood serum were determined respectively by ELISA. The change in the content of nitric oxide (NO) in blood serum was determined with colorimetry. The content of malondialdehyde (MDA) in left ventricle was assayed with spectrophotometry. The contents of lactate dehydrogenase (LDH) and creatine kinase (CK) in blood serum were detected with automatic biochemistry analyzer. Compared with the control group, the myocardial indexes, the contents of I , III collagen quantification, angiotensin II in blood serum and malondialdehyde in left ventricle were markedly increased and the content of nitric oxide in blood serum was decreased, the contents of lactate dehydrogenase and creatine kinase in blood serum were markedly increased in Iso model group (P<0.05 or P<0.01). Compared with the model group, the myocardial indexes were decreased, the contents of I , III collagen quantification, angiotensin II in blood serum were reduced in PC 200 and 400 mg x kg(-1) x d(-1) groups (P<0.05 or P<0.01). The content of nitric oxide in blood serum was increased, the content of malondialdehyde in left ventricle was markedly decreased, the contents of lactate dehydrogenase and creatine kinase in blood serum were markedly decreased in PC three groups (P<0.05 or P<0.01). PC could inhibit collagen synthesis by decreasing angiotensin II level and increasing the content of nitric oxide and antioxidation, and thereby inhibiting myocardial fibrosis and protect myocardium in rats.