Ovo Injection Research Articles

In ovo injection of organic zinc effects on hatching rate of broiler breeder hen eggs and productivity of broilers

The aim of this study was to investigate the effect of in ovo injection of organic zinc on the characteristics of broiler chickens and the performance of broiler chickens A total of 320 eggs from Ross 308 strain with an average weight of 65 grams were randomly divided into four groups, negative control (no injection), positive control (in ovo injection of 0.272 ml of normal saline solution) and two experimental groups, eggs in the first received in ovo injection of 0.272 mg of organic zinc whereas in the second the eggs were injected with 0.544 mg of the same organic zinc. The injection was done in the amniotic sac on the 10th day of incubation. The effect of injection on hatching traits and then on functional traits, blood parameters, immune response, carcass characteristics and cecal microbial population of resulting broilers were measured. The results showed that the percentage of hatched broiler chickens in the zinc-treated groups was significantly lower compared to the two control groups. However, the intraocular injection of zinc caused a significant increase in the final weight gain of the birds and their feed consumption. Blood parameters were also affected in experimental treatments and blood glucose increased and blood triglyceride, cholesterol and lipoproteins decreased compared to negative and positive control (P<0.01). On the other hand, no statistically significant difference was observed in the immune response, microbial population and carcass characteristics among the experimental groups. In conclusion, the intra egg injection of organic zinc can show favorable results in the performance indicators of broiler chickens, although it had no effect on the immune response and microbial population.

Effects of In Ovo Administration of Freeze-Dried Royal Jelly on Hatchability, Blood Parameters, and Organ Weights of Day-Old Chicks.

Royal jelly renowned for its robust nutritional, functional, and biological properties, is a pivotal product derived from honeybees. The purpose of this investigation was to assess the theory that in ovo injection of freeze-dried royal jelly (FDRJ) solutions at varying concentrations can influence the hatchability, blood properties and hatching characteristics of day-old chicks. A total of 480 eggs (54.81 ± 0.187 g) were allocated into four experimental groups: negative control (NC), without injection, positive control (PC), administered with regular saline, a low FDRJ dose group (9 mg/egg), and a high FDRJ dose group (18 mg/egg). The in ovo injections were administered on Day 18 of incubation, and the experiment was subsequently continued until the incubation period concluded at 21 days. Results revealed that the lower FDRJ dose (9 mg/egg) significantly improved hatchability percentages compared to other treatments. Conversely, the higher FDRJ dose (18 mg/egg) and control groups (NC and PC) resulted in significantly higher chick yield percentages than the lower FDRJ and PC groups. The NC group showed the supreme yolk sac (YS) percentage, whereas the yolk-free body mass (YFBM) percentages displayed an inverse trend. Furthermore, the in ovo FDRJ injection did not affect haematological values or the relative organ weight of day-old chicks. In conclusion, in ovo FDRJ injection demonstrated beneficial effects on hatchability and chick weight, as evidenced by the studied parameters.

The Impact of In Ovo Injection of Organic Manganese on Broiler Breeder Hen Egg Hatchability and Progeny Broilers Productivity

The Impact of In Ovo Injection of Organic Manganese on Broiler Breeder Hen Egg Hatchability and Progeny Broilers Productivity

Enhancements in the expressions of genes associated with the immunity, muscle growth, and antioxidant activity of 14 d broilers in response to the in ovo injection of the Marek's disease vaccine alone or in conjunction with in ovo and dietary supplemental administration of 25-hydroxycholecalciferol

Influences the Marek's disease vaccine (MDV) alone or combined with the in ovo and dietary administration of 25-hydroxycholecalciferol (25OHD3) on the expression of genes associated with the breast muscle deposition, adaptive and innate immunity, and antioxidant and vitamin D activities of 14 d-old broilers were investigated. Four in ovo treatments were: non-injected; commercial MDV-alone-injected (50 μl); or 50 μl of MDV containing 1.2 (MDV+25OHD3-1.2) or 2.4 (MDV+25OHD3-2.4) μg of 25OHD3. Two dietary treatments were a commercial diet containing 25OHD3 (250 IU)/kg of feed (control) or the same diet supplemented with additional 25OHD3 (2,760 IU)/kg of feed (Hy-D diet). One bird per pen (48 total) was sampled at 14 d for determination of the expression of genes involved with the muscle deposition (MyoD1, MyoG, Pax3, and Mrf4), immunity (INF-γ, IL-10, IL-8, IL-1β, and TGF-β4), antioxidant capacity (SOD1, SOD2, GSH-P1, GSH-P7, and CAT), and vitamin D activity (VDR, 1α-hydroxylase, and 24-hydroxylase) in the spleen and pectoralis major (P.major) muscle. The treatment differences were considered significant at P ≤ 0.05. In the P. major, Mrf4 and MyoG were up-regulated in Hy-D-fed birds. Also, the in ovo and dietary 25OHD3 sources individually increased SOD2 gene expression in the P. major. In the spleen, the expressions of IL-1β and IL-8 were down-regulated and IL-10 and TGF-β4 gene expressions were up-regulated in Hy-D-fed birds than those commercial-fed broiler. In ovo and dietary 25OHD3 sources enhanced vitamin D gene (1α-hydroxylase and 24-hydroxylase) activities in the breast and spleen. In ovo x dietary treatment interactions were significant for the MyoD1, IL-8, Pax3, TGF-β4 genes of the P. major, in which the combined MDV with 1.2 μg of 25OHD3 enhanced their expressions in birds fed the Hy-D diet. In conclusion, both 25OHD3 sources promoted the expression of genes associated with immunity and P. major growth. It is recommended that both 25OHD3 sources can be used to promote the gene expression of 14-d-old broilers in the spleen and breast muscle when MDV administered in ovo.

Evaluation of d 18 in ovo administration of soursop (Annona muricata) leaf extract into the air space on hatch performance and physiology of Noiler chicks

Efficient poultry production can be accomplished using combined technologies. A combination of in ovo and ethno-veterinary technologies can deliver significant benefits, including reduced labour and production costs. This study evaluated the effect of in ovo administration of soursop leaf extract (SLE) on the hatching performance and physiology of Noiler chicks. A total of 550 eggs were incubated, and 460 fertile eggs were randomly distributed into 4 groups with 3 replicates on the 18th d of incubation. The eggs were then injected with 0.75 µg SLE, 1.5 µg SLE, and saline solution (negative control) at a rate of 0.2 ml in the air space. The noninjected group served as the positive control. At the end of the hatching period, the various groups were evaluated for embryo mortality, hatchability, hatch duration, organ weight, serum biochemistry, and chick quality. The results showed no significant differences (P > 0.05) in embryonic mortality, hatchability, organ weight, total protein, albumin, globulin, aspartate aminotransferase, alanine aminotransferase, and glucose among the treatment groups. However, chick weight, chick quality, and serum triglyceride levels were significantly (P < 0.05) higher in the extract-injected group. Additionally, incubation and hatch times were significantly lower (P < 0.05) in the SLE group compared to the other groups. In ovo administration of soursop leaf extract resulted in reduced incubation duration, hatch time, and embryo mortality. In conclusion, the in ovo injection of SLE improved hatch performance and chick quality.

Effects of in ovo supplementation of selenium (Se) and zinc (zn) on hatchability and production performance of broiler chickens.

The current research was conducted to assess the effect of in ovo feeding (IOF) of selenium (Se) and zinc (Zn) on hatchability, production performance, liver, intestinal morphology, antioxidant levels and expression levels of immune-related genes in broiler chickens. A total of 400 fertilized eggs were equally divided into four groups: control (non-injected), sham (in ovo injection of 0.75% NaCl), Se (@ 1.5µg/egg in ovo injection) and Zn (500µg/egg in ovo injection) groups respectively. On the seventeenth day of incubation, treatment solutions were administered into amniotic fluid of fertilized eggs. The results revealed that Se and Zn supplementation significantly (P < 0.05) enhanced hatchability, post-hatch growth, organ development, and liver antioxidant capability. Histopathological examination revealed a typical hepatocyte morphology, well-arranged cells, and a significant (P < 0.05) decrease in apoptosis in both selenium and zinc groups. Additionally, selenium and zinc produced auspicious effects on intestinal epithelium and villi surface area. Interestingly, our results revealed that IOF of Se and Zn modulated the expression of immune-related genes in comparison to the control and sham groups. Conclusively, IOF of Se and Zn augmented health and productivity by enhancing the cellular immunity in the broiler chickens, thus IOF can be utilized as an effective strategy to promote health and immunity in broiler chickens.

Effect of Pyridoxine in Ovo Injection in Broiler Hatching Eggs on Some Physiological Parameters

Effect of Pyridoxine in Ovo Injection in Broiler Hatching Eggs on Some Physiological Parameters

Evaluating the interaction response of broiler chickens to in ovo feeding of Citrus aurantiifolia seed extract and its powder supplementation

This study aimed to evaluate the effect of in ovo injection and post-hatch supplementation of Citrus aurantiifolia seed on hatchability, growth performance, antioxidant capacity, immune organs weight, and intestinal morphology of Cobb 500 broilers chickens. On day 18 of incubation, 450 fertile eggs were randomly allocated into three sets: non-injected group, 0.5 µg/ml Citrus aurantiifolia seed extract (CASE) group and 0.75 µg/ml CASE group. After hatch, each set was allocated into two groups (5 replicates of 10 birds/replicate each) and was fed on a standard diet with or without Citrus aurantiifolia seed powder (CASP) supplementation (5 g/kg). The groups were divided as follows: IoCA0-C, IoCA0-CA, IoCA5-C, IoCA5-CA, IoCA75-C and IoCA75-CA where IoCA0-C was: non-injected group fed standard diet (negative control group), IoCA0-CA: non-injected and fed CASP supplemented diet (positive control group), IoCA5-C: 0.5 µg/ml of CASE-injected and fed standard diet, IoCA75-C: 0.75 µg/ml of CASE-injected and fed standard diet, IoCA5-CA: 0.5 µg/ml of CASE-injected and fed CASP supplemented diet and IoCA75-CA: 0.75 µg/ml of CASE-injected and fed CASP supplemented diet. The results revealed that the interaction between CASE in ovo injection at 0.5 µg/ml and dietary CASP supplementation at 5 g/kg in the diet increased body weight and weight gain (p < 0.001). The interaction between CASE in ovo injection and dietary CASP supplementation decreased the serum malondialdehyde concentration, the relative weight of the thymus and improved duodenum and ileum villus height (p < 0.05). In conclusion, in ovo injection and dietary supplementation of Citrus aurantiifolia seed powder can improve growth performance and the intestinal morphology of broiler chickens.

In ovo injection of bee pollen extract on hatchability, chick quality, glycogen reserves and production performance in broiler chickens

This study aimed to investigate the effects of in ovo injection of bee pollen (BP) extract on some hatching traits, glycogen reserves and production performance in broilers. A total of 886 eggs was randomly assigned to 5 treatments: the 0.9% NaCl diluent-injected control group, and the groups that were injected with BP extract at 3 different concentrations: BP-1.5%, BP-2.5% and BP-5.0% (7, 12 and 25 mg/egg, respectively). The last group received a carbohydrate solution (CS). At 18 d of incubation, 500 µL of each solution was injected into the air sac of each egg of the injected groups. After hatching, a total of 570 one-day-old chicks were distributed into 5 groups (in ovo injection) with 3 replicates with 38 birds. The in ovo injection of BP at a dose of 5% and CS resulted in lower hatching rates (P < 0.05) as compared with the control group. The level of glycogen in the muscle tissue of newly hatched birds was not significantly different (P > 0.05). Supplementation of embryos with BP extract also caused a significant increase in the length of chicks in the BP-1.5% group (P < 0.001). Hatched chicks from the injected eggs had a lower liver glycogen content than that of the control. Based on the results, it was concluded that high-quality day-old chicks could be obtained by in ovo injection. Supplementation of chicks with 1.5% BP extract had a beneficial effect on BW during the first rearing period and showed a hypocholesterolemic effect in young chicks. At the same time, an adverse effect of a high dose of BP (5%) and a carbohydrate solution administered in ovo on the hatchability level of chicks was shown. The implementation of the carbohydrate mixture resulted in a deterioration of biochemical indices in the plasma of newly hatched chicks, especially in the case of transaminase activity.

Chemerin abundance in egg white and its expression with receptors in extra-embryonic annexes of Pekin ducks: implications for embryo development

Embryonic mortality is a significant problem in the commercial duck industry worldwide. Therefore, identification of new biomarkers for duck embryo development is necessary. In the chicken (order Galliformes), we previously showed that chemerin is a hormone locally produced by the reproductive tract in hens, particularly in the magnum area, leading to its accumulation in the egg white and within the embryo annexes during embryonic development. We therefore hypothesized that the chemerin concentration in egg white could be a biomarker of egg performance and reproductive parameters in Pekin ducks (order Anseriformes). Thus, we collected eggs from Pekin ducks over a 5-day period at three stages of the laying period (before the laying peak, after the laying peak, and at the end of the laying period) to measure the chemerin concentrations in egg white by enzyme-linked immunosorbent assay. The chemerin concentration in egg white decreased during the laying period and was not associated with reproductive parameters. We found negative correlations between the chemerin level in egg white and the albumen weight. Reverse-transcriptase quantitative polymerase chain reaction showed that chemerin and its three receptors CMKLR1, GPR1, and CCRL2 were expressed in the reproductive tract and within allantoic and amniotic annexes during embryo development. Chemerin concentrations strongly increased in amniotic fluid on embryonic day 16 (ED16) when the egg white was transferred into the amniotic sac. Finally, chemerin inhibition in egg white by in ovo injections of anti-chemerin antibodies (0.01, 0.1, and 1 µg) increased the embryo mortality rate. These data demonstrate the important role of the chemerin system during egg formation and embryo development in Pekin ducks, suggesting their potential use as biomarkers for determining the quality of poultry eggs and embryo development.

Benefit and potential risk: Effects of in ovo copper oxide nanoparticles supplementation on hatchability traits, organ weightsand histological features of newly hatched chicks.

This investigation was directed to examine the influence of copper oxide nanoparticles (CuO-NPs) on the hatchability traits, and chick quality of newly hatched broiler chicks. A total of 480 eggs were randomly divided into four treatment groups, each consisting of three duplicates. As a negative control (NC), the first group was not injected; the second group was injected with saline and served as a positive control (PC), the third and fourth groups were injected with 30 and 60 ppm of (CuO-NPs)/egg. Eggs were injected into the amniotic fluid on the eighteenth day of the incubation period. Results showed that the hatchability, chick yield %, yolk free-body mass (YFBM), chick length, shank length (SL), and relative weight of the heart, gizzard and intestine of day-old broiler chicks were all unaffected by the in ovo injection of CuO-NPs. The Pasgar Score was slightly improved compared to the NC and PC groups. Also, the in ovo administration of CuO-NPs (60 ppm/egg) significantly increased the intestine length. Both levels of CuO-NPs significantly increased the concentration of Cu ions in the hepatic tissue. Additionally, different levels of tissue damage were seen in the liver of the birds that were given low or high dosages of CuO-NPs. Conclusively, the in ovo injection of CuO-NPs has a good result on the appearance of the chicks (Pasgar score). However, negative effect of CuO-NPs on liver tissue may raise concerns about the potential risks of applying CuO-NPs in ovo administration.

Effect of different synbiotic administration methods on growth, carcass characteristics, ileum histomorphometry, and blood biochemistry of Cobb-500 broilers.

To combat enteric infections and antibiotic resistance in the poultry industry, researchers seek alternatives such as probiotics, prebiotics, and synbiotics as growth promoters. Synbiotics support probiotic growth through the supply of essential nutrients. The study's objectives were to assess the most effective delivery methods for synbiotics and evaluate their growth, histomorphometric, and hematological impacts on Cobb-500 broilers. Two studies, independently conducted, employed a completely randomized design. One hundred and eighty viable eggs in the first trial were assigned to three groups: Control (T1), sterile water (T2), and synbiotic in sterile water (T3). On the 21st day of hatching, hatchability, day-old body weights, and ileum samples for histomorphometric analysis were recorded. In the second trial, out of 500 viable eggs, 200 eggs were fed in ovo with synbiotics (PoultryStar® sol, Biomin Singapore Pte Ltd, Singapore) on 17.5 days and 300 were set aside without in ovo injection. The treatments were control (T1), in water synbiotic (T2), in ovo synbiotic (T3), combination of in ovo synbiotic and synbiotic in feed (T4), and synbiotic in feed only (T5). On 21 and 42 days, blood, ileum, and visceral organ samples were collected for laboratory analysis. Data on weight gain, daily feed intake, and water consumption were recorded for 42 days. The initial experiment's results revealed a decrease in hatchability, slight weight increase, and significant intestinal morphological changes with the use of an in ovo synbiotic. Applying synbiotic through various methods in the second trial yielded better growth results, lower blood cholesterol, and significantly longer (p < 0.05) villi on 21 days. Using the in ovo method to administer synbiotics lowered hatchability. Use of synbiotics with any method or in combination enhances growth, ileum structure, dressing yield, feed efficiency, and cholesterol levels in blood. Synbiotics enhance gut health and overall performance in broilers when used through diverse approaches.

Effects of the Marek's Disease Vaccine on the Performance, Meat Yield, and Incidence of Woody Breast Myopathy in Ross 708 Broilers When Administered Alone or in Conjunction with In ovo and Dietary Supplemental 25-Hydroxycholecalciferol.

The effects of the Marek's disease vaccine (MDV) on the live performance, breast meat yield, and incidence of woody breast myopathy (WBM) of Ross 708 broilers were investigated when administered alone or in conjunction with in ovo and dietary supplemental 25-hydroxycholecalciferol (25OHD3). At 18 d of incubation (doi), four in ovo injection treatments were randomly assigned to live embryonated Ross 708 broiler hatching eggs: (1) non-injected; (2) commercial MDV alone; or MDV containing either (3) 1.2 or (4) 2.4 μg of 25OHD3. An Inovoject multi-egg injector was used to inject a 50 μL solution volume into each egg. The birds were provided a commercial diet that contained 250 IU of cholecalciferol/kg of feed (control) or a commercial diet that was supplemented with an additional 2760 IU of 25OHD3/kg of feed (HyD-diet). In the growout period, 14 male broilers were placed in each of 48 floor pens resulting 6 replicated pens per in ovo x dietary treatment combination. Live performance variable were measured at each dietary phases from 0 to 14, 15 to 28, and 29 to 40 d of age (doa). At 14 and 40 doa, pectoralis major (P. major) and pectoralis minor (P. minor) muscles were determined for one bird within each of the six replicate pens. At 41 doa, WBM incidence was determined. No significant main or interaction effects occurred for WBM among the dietary or in ovo injection treatments. However, in response to in ovo 25OHD3 supplementation, BW and BWG in the 29 to 40 doa period and BWG and FCR in the 0 to 40 doa period improved. In addition, at 40 and 41 doa, breast meat yield increased in response to in ovo and dietary 25OHD3 supplementation. Future research is needed to determine the possible reasons that may have been involved in the aforementioned improvements.

Effects of in ovo injection of organic selenium on the hatchability of broiler breeder hen eggs and resulting chick physiology and performance.

Selenium is an essential mineral for poultry. The conflicting reports about its in ovo injection are the justification for the more detailed investigation. The aim of this study was to investigate the effects of in ovo injection of organic selenium on the hatching traits of broiler chickens and their performance. Three hundred and twenty eggs of Ross 308 strain with an average weight of 65g and 160 chicks were randomly divided into 4 treatment groups (each with 8 replicates of 10 eggs each for hatching parameters and 4 replicates of 10 chicks for broiler farming parameters): negative control (no injection), positive control (in ovo injection of 0.272mL of normal saline solution) and 2 selenium treatments (in ovo injection of 2.72 or 5.44μg of organic selenium). Injection was into the amniotic sac on the 10th day of incubation. Effects of in ovo injection on hatching and performance traits, blood parameters, immune responses, carcass characteristics, meat fatty acid profile, cecal microbial population and selenium consternation in the tibia were measured. Fewer chicks from the injected treatments hatched than from the negative control group (p<0.01). However, the injection of selenium increased feed intake and the final weight of the birds (p<0.01). Blood parameters were also affected. Glucose and cholesterol in experimental treatment chicks was lower than those of the controls (p<0.01), whereas blood lipoproteins (VLDL, LDL and HDL) and the ratio of cholesterol to HDL was significantly increased in the treatments injected with selenium (p<0.01). There was no significant difference in the immune response or microbial population between the experimental groups, but carcass components, such as thigh, breast, wing and abdominal fat weight, were significantly greater in the selenium treatments. Intra-egg injection of organic selenium produced favourable effects on performance of broiler chickens, although it had no effect on immune response or microbial population. However, the negative effect on hatching of chickens needs to be prevented to result in an acceptable economic return for the producer.

In ovo feeding of α-ketoglutaric acid improves hepatic antioxidant-gene expression, plasma antioxidant activities and decreases body temperature without affecting broiler body weight under cyclic heat stress

The broiler industry is adversely affected by the rise in global temperature. This study investigated the effects of in ovo feeding of α-ketoglutaric acid (AKG) on growth performance, organ weight, plasma metabolite, plasma oxidative stress, rectal temperature (RT), and hepatic mRNA expression of antioxidant-related genes in Arbor Acres broilers subjected to cyclic heat stress (HS). Three hundred fifty fertile eggs during incubation were divided into 5 groups according to AKG concentrations and temperature conditions. After dissolving AKG in distilled water at 0, 0.5, 1.0, and 1.5, 0% AKG was in ovo administered to 2 of the 5 groups whereas the remaining 3 groups received 0.5, 1.0, and 1.5%, respectively. From d 29 to 34 of age, 4 groups of birds received heat stress (HS) at 31°C ± 1°C for 6 h per day while the other group was kept at room temperature (21°C ± 1°C; NT). So, the 5 treatment groups were: 1) 0AKG-NT, where chicks hatched from eggs receiving 0% AKG were reared under thermoneutral conditions. 2) 0AKG-HS, where chicks hatched from eggs receiving 0% AKG were reared under cyclic HS conditions. 3) 0.5AKG-HS, where chicks hatched from eggs receiving 0.5% AKG were reared under cyclic HS conditions. 4) 1.0AKG-HS, where chicks hatched from eggs receiving 1.0% AKG were reared under cyclic HS conditions. 5) 1.5AKG-HS, where chicks hatched from eggs receiving 1.5% AKG were reared under cyclic HS conditions. HS significantly reduced body weight change (ΔBW %) and average daily gain (ADG) without affecting average daily feed intake (ADFI). Feed conversion ratio (FCR) was significantly increased (P = 0.003) in all HS-treated groups. A significant linear decrease in the final RT (P = 0.005) and a change in RT (P = 0.003) were detected with increasing AKG concentration. Total antioxidant capacity (P = 0.029) and antioxidant balance (P = 0.001) in plasma increased linearly with increasing AKG concentration whereas malondialdehyde concentrations were linearly decreased (P = 0.001). Hepatic gene expression of CAT (P = 0.026) and GPX1 (P = 0.001) were dose-dependently upregulated while nicotinamide adenine dinucleotide phosphate oxidase (NOX)1, NOX4, and heat shock protein (HSP)70 were linearly downregulated (P < 0.05). Hence, in ovo injection of AKG was effective in mitigating HS-induced oxidative stress without attenuating the adverse effects on broiler growth.

Effect of In Ovo Injection with Ascorbic Acid on Hatchability, Hatching Weight and Muscular Gain in Broiler Chickens.

Effect of In Ovo Injection with Ascorbic Acid on Hatchability, Hatching Weight and Muscular Gain in Broiler Chickens.

Effects of In ovo Injection of L-carnitine and Betaine on Hatchability, Blood Metabolites Concentration, Carcass Characteristics, Expression of Some Growth Associated Genes and Development of One Day Old Chicks

Effects of In ovo Injection of L-carnitine and Betaine on Hatchability, Blood Metabolites Concentration, Carcass Characteristics, Expression of Some Growth Associated Genes and Development of One Day Old Chicks

Influence of in ovo administration of oyster mushroom extract on morphological and histological responses of intestines of broiler chickens.

This study investigated the influence of in ovo administration of oyster mushroom aqueous extract on intestinal development of broiler chickens. A total of 600 hatching eggs of Arbor acre strain of broiler chickens were used in this experiment. The eggs were divided into three treatments; T1: Control (non-injected eggs), T2: in ovo administration of 0.1 ml of oyster mushroom aqueous extract and T3: in ovo administration of 0.2 ml of oyster mushroom aqueous extract. In ovo injection of eggs was carried out on the 18th day of incubation and hatched chicks were brooded for 2 weeks and the experiment lasted for six weeks. At the starter and finisher (7th and 56th days of age) phases, two birds per replicate were randomly selected for intestinal evaluation. Data collected were subjected to Analysis of Variance using General Linear Model. Results from the starter phase showed jejunal villus height significantly (p&lt;0.05) ranged from 290.62 µm in birds in T1 to 415.60 µm in birds in the T3 group. Moreover, jejunal crypt depth ranged from 51.96 µm to 115.62 µm, while muscular wall thickness ranged from 53.94 µm to 139.23 µm with the highest recorded in the T2 birds. In finisher broilers, there was significant (p&lt;0.05) difference among treatments for duodenum length, muscular wall thickness of the ileum and colon length. Histological plates taken in the starter phase indicated no visible lesion in the villi across treatments with crypts appearing normal. However, finisher broilers in the T2 group had preeminent villi representation showing intact enterocytes and slightly high and more prominent crypts. Therefore, to improve jejunal morphology in the starter phase as well as increased intestinal length and superior duodenal histology in the finisher phase of broiler chickens, in ovo administration of 0.1 ml oyster mushroom aqueous extract is recommended.

Effect of in ovo injection of epigallocatechin-3 gallate and oleuropein on hatching, productive and physiological aspects of broiler chicks exposed to short heat stress

This study was aimed to investigate the influence of in ovo injection (IO) into air cell (AC) or yolk sac (YS) of epigallocatechin-3 gallate (EG) and oleuropein (OL) as antioxidants on hatching, physiological and productive performance of heat-stressed chicks. 840 fertile eggs were chosen for IO on 12th day of incubation. Eggs were divided into 7 groups within 4 replications each as follows: negative control (NC) without IO and other groups involved IO of 100 μl distilled water and 5 mg each of EG and OL in both AC and YS sites of egg. After hatching, chicks were exposed to heat stress for 24 h and raised for 42 d. Compared with NC, the results revealed that main effect of IO with EG and OL increased (p≤0.05) hatchability and decreased deformed chicks and serum levels of glucose, cholesterol, aspartate aminotransferase, corticosterone and heat shock protein 70 with enhancing feed efficiency, superoxide dismutase, glutathione peroxidase and thyroxine in serum or liver. High chick length and relative chick weight with low serum protein carbonyl and feed intake were recorded (p≤0.05) by EG. Low rectal temperature and heterophil to lymphocyte ratio with high body weight were recordered (p≤0.05) by OL. No mainly altered effects between both IO sites on most variables measured. However, there were significant influences among interactive treatments which related to in ovo injected substance in an injection site-dependent manner. It is concluded that improved hatchability, physiological and productive characteristics of heat-stressed chicks were achieved by IO of EG and OL.

In ovo injection of AZD6244 suppresses feather follicle development by the inhibition of ERK and Wnt/β-catenin pathways in goose embryos (Anser cygnoides)

ABSTRACT 1. Feathers are an important product from poultry, and the state of feather growth and development plays an important role in their economic value. 2. In total, 120 eggs were selected for immunoblotting and immunolocalisation experiments of ERK and β-catenin proteins in different developmental stages of goose embryos. The ERK protein was highly expressed in the early stage of goose embryo development, while β-catenin protein was highly expressed in the middle stage of embryo development. 3. The 120 eggs were divided into four treatment groups, including an uninjected group (BLANK), a group injected with 100 µl of cosolvent (CK), a group injected with 100 µl of AZD6244 containing cosolvent in a dose of 5 mg/kg AZD6244 containing cosolvent (AZD5) and a group injected with 100 µl of AZD6244 containing cosolvent in a dose of 15 mg/kg AZD6244 containing cosolvent (AZD15). The eggs were injected on the ninth day of embryonic development (E9). Samples were collected at E21.5 to observe feather width, feather follicle diameter, ERK and Wnt/β-catenin pathway protein expression. 4. The AZD5 and AZD15 doses were within the embryonic safety range compared to the BLANK and CK groups and had no significant effect on the survival rate and weight at the inflection point, but significantly reduced the feather width and feather follicle diameter (p < 0.05). The AZD6244 treatment inhibited ERK protein phosphorylation levels and blocked the Wnt/β-catenin pathway, which in turn significantly down-regulated the expression levels of FZD4, β-catenin, TCF4 and LEF1 (p < 0.05), with an inhibitory effect in the AZD15 group being more significant. The immunohistochemical results of β-catenin and p-ERK were consistent with Western blot results. 5. The small molecule inhibitor AZD6244 regulated the growth and development of feather follicles in goose embryos by the ERK and Wnt/β-catenin pathways.