Introduction: Vaginal discharge is a common problem among many women and causes unpleasant odor and discomfort. Therefore, the study was conducted on a group of these women to find out the microbial content causing this problem and to conduct a drug sensitivity test. Methods and Materials: Specimens were collected from 92 non-pregnant women, aged (20-60) years, from the city of AL-Najaf in Iraq. 78 (84.78%). Results: Samples were positive, and the largest percentage of microbial content was for fungi represented by yeasts of Candida spp. in the rate of 54%. C. albicans was the most prevalent kind by 15%. The remainder is made up of non-Candida albicans kinds. Which is C. glabrata, C. Ciferrii, C. Guilliermondii, C. Tropicals, C. krusi . This was followed by gram-positive bacteria at a rate of 28%, This percentage was distributed between Streptococcus pyogenes by 15% and the rest for other types of bacteria Staphylococcus Spp. While Gram-negative bacteria accounted for the least percentage of the total positive specimens, at 18%, distributed between Klebsiella spp.13% and E. coli, 5%. As for the drug sensitivity test, C. glabrate, C. ciferrii and C. Krusi showed resistance to fluconazole (MIC= 8) and were sensitive to Voriconazole. Both Gram-positive and Gram-negative bacteria showed various drug sensitivity. Conclusion: This study showed that there are two main causes of vaginal infections: first: an overgrowth of Candida yeasts, and second: some types of bacteria, which are similar to those that cause urinary tract infections, as these bacteria are transmitted between the vagina and the urinary canal due to the closeness of the two areas to each other in women.