Overall Prevalence Of Parasitism Research Articles

Assessments and Identification of Selected Fish-Borne Zoonotic Parasites in Nile Tilapia and African Catfish Species in Lakes of Haramaya District, Ethiopia

Global fish consumption increased higher than that of all other animal protein foods. The image of fish as a healthy food is the main reason for increasing demand for fish meat, but there are serious safety concerns related to the presence of fish-borne zoonotic pathogen including parasites. A cross-sectional study was conducted from December 2021 to July 2022 at Lake Haramaya, Lake Tinike, and Lake Adele on Oreochromis niloticus and Clarias gariepinus fish species to determine the prevalence of Contracaecum (Nematoda) and Clinostomum (Trematoda) infection. A total of 384 individuals of C. gariepinus 241 (62.76%) and O. niloticus 143 (37.24%) were sampled from Lake Haramaya 149 (38.8%), Lake Tinike 113 (29.4%) and Lake Adele 122 (31.8%). The overall prevalence of parasite was 30.2% (116/384) and higher overall prevalence of parasite was recorded in Lake Haramaya (36.2%) followed by Lake Adele (27.9%) and Lake Tinike (24.8%). C. gariepinus was more affected than O. niloticus fish and Contracaecum parasite was more prevalent than Clinostomum parasite (37.3% and 18.2%) and (29.9% and 14.6%), respectively. The distribution of parasite was significantly affected with respect to species of fish, sex, weight, and length categories, genera of parasite, and location of parasites. The perception of respondents on fish-borne zoonotic parasite (FBZP) was affected by demographic characteristics of respondents. Human and animals were interacted with the lakes and its environment adversely which can perpetuate the life cycle of FBZP. Moreover, unsafe fish meat processing from harvesting to consumption was the main problem at the study area, which can makes surrounding people under the risk of FBZP. However, the risks associated with FBZP can be reduced through the application of good hygiene, fishing practices, inspection of fish meat, proper storage, adequate cooking of fish, and with the promotion of the community awareness.

Gastrointestinal Helminth Parasites of Malapterurus electricus from Anambra River, Nigeria

Aim: One of the constraints of fish in the wild for sustainable productivity is parasitic infections. The study was carried out to determine the gastrointestinal helminth parasites associated with Malapterurus electricus and their prevalence in relation to sex and size.
 Study Design: The study was a survey done to establish the type of gastrointestinal helminth parasites found in M. electricus.
 Place and Duration of Study: The specimens were collected from Anambra River between August and December 2018.
 Methodology: A total of thirty-two (32) fishes were examined through dissection and observation of the alimentary canal under the objective lens of the microscope. The chi-square test was employed to determine the possible association between parasite prevalence, the sexes, and size. Results: The overall prevalence of parasites was 56.25%. The parasites recovered were, Electrotaenia malapteruri (50.00%) and Tenuisentis niloticus (6.25%). The male specimens (18) recorded a prevalence of 66.67% while the female (14) recorded a prevalence of 42.86%. There was no significant difference in the prevalence of gastrointestinal helminths among gender (P=0.178, P>0.05). The length group, 30 – 39cm had a 100% prevalence. Thus, a significant difference between the fish size and parasite prevalence was observed. The weight groups, 100 -199g, and 400 - 499g also had a 100% prevalence. However, no significant relationship was observed between fish weight and parasite prevalence. The intestine was infected with parasitic worms of the cestode and acanthocephalan species.
 Conclusion: This study showed that there is high prevalence of gastrointestinal helminth parasites in M. electricus and this may possibly affect the fish quality.

Evaluation of Three Deworming Methods and their Long-Term Effect on the Weight of Buffaloes Raised in Silvopastoral Systems

Purpose of the Study: The present work aimed to evaluate the effect of three deworming methods on body weight gains in buffaloes raised in silvopastoral systems (SPS).
 Methods: Thirty buffaloes were divided into three groups (ten per group) as follows: 1) Albendazole 10 g + 1.3 g of cobalt sulfate at one week of age and 14 days later; 2) Albendazole 10 g + 1.3 g of cobalt sulfate at one week of age and 14 days later, then at 6 months and 14 days later; 3) Ivermectin 1 g at 30 days of age and then at six months of age. Birth, weaning (9 months), and final (18 months) weight were registered, and daily weight gains were calculated. The McMaster technique was used to evaluate the presence of parasites.
 Results: The overall prevalence of parasites in the animals evaluated was 93.33%. The most common parasites were: Strongylus sp., Neoascaris sp., Moniezia sp., and Eimeria sp. Before the application of the dewormers, initial values in G1, G2, and G3 were 360, 350, and 210 hpg/opg; after the application of the treatments, 60, 25, and 20 hpg/opg were obtained, respectively. Buffaloes in G2 showed significantly (P=0.046) high final weights (415.10±23.76 kg) compared to G1 (354.80±46.71 kg) but showed no difference with G3 (374.80±43.60k).
 Conclusion: It is concluded that albendazole at 10 g and a repeat at six months of age can be used in buffalo breeding in SPS, which could help to implement regenerative livestock programs, decreasing the use of Ivermectin.

Further Studies on Helminth Parasites of Fish in Asa Dam, Kwara State, Nigeria

Studies on parasites in fish have been a great concern for over a decade now due to disease conditions, overall nutritive devaluation and huge economic loss that resulted from parasitic infections. Added to the potential of infected fish to be a source of infections to man and other animals. The present study is, however, designed to ascertain the parasitic infection in fish from Asa Dam, Kwara State, Nigeria. Atotal number of 196 fish belonging to 10 families and 18 species were examined for helminth infection with an overall prevalence rate of 31.10%. Parasites were recovered from the skin, gills, body cavity, intestine, and liver of the fish host. Trematode was the most prevalent parasite group found in the fish host and represented 79.5% of the isolated parasites, followed by Cestode (7.14%), and Acanthocephalan was the least group (0.4%) recovered. Most of the Trematodes were recovered from T. zillii and H. fasciatus (109 and 56 respectively), while C. anguillaris, C. senegalensis, C. gariepinus and C. obscura have no cases of trematode. 2.55% of the cestodes were recovered from C. gariepinus while the overall index of infection was significantly high in T. zillii (48.90%) and H. fasciatus (40.44%). The overall prevalence of parasites examined in relation to sex indicates that males had 33.7% females had 28.4% prevalence accordingly with no significant difference (P> 0.05). This finding indicates that there is a high parasitic load as evident by the infection rate on the examined fishes, as such adequate measures should be put in place to reduce and prevent the parasites from spreading.

Effect of urbanization on zoonotic gastrointestinal parasite prevalence in endemic toque macaque (Macaca sinica) from different climatic zones in Sri Lanka.

Understanding variations in host-parasite relationships with urbanization is vital for both, public health management and conservation of endemic animals with high anthropogenic interactions. Toque macaques (Macaca sinica) are such endemic old-world monkeys in Sri Lanka. Three macaque sub species inhabit the main climatic zones of the island; M.s. sinica,M.s.aurifrons and M.s.opisthomelas inhabit the dry zone, wet zone, and montane regions of the island, respectively. This study aimed to examine parasite prevalence in this host in association with urbanization. A total of 180 fecal samples were collected from the three sub species of toque macaques inhabiting the main climatic zones (dry, wet, and montane) in Sri Lanka; 20 samples each were collected from urban, suburban, and wild populations representing each climatic zone. Twenty gastrointestinal (GI) parasite genera types i.e. five types of protozoan cysts, two types of trematode ova, four types of cestode ova, eight types of nematode ova, and a single type of acanthocephalan ova were identified. The overall prevalence of parasites was 62% (112/180) with the highest prevalence of Strongyloides infection. In all three sub species, toque macaque populations with proximity to human settlements, including urban and suburban populations, manifested a greater GI parasitic prevalence, mean ova/cyst counts and species richness, compared to their wild counterparts. Importantly, records of five parasite types in toques in Sri Lanka are reported for the first time, while Moniliformis type was reported as a first record in free ranging macaques, globally. This study clearly demonstrated that human contact and habitat modification may influence patterns of parasitic infections in macaques. As most of the parasite types identified manifest zoonotic potential, with public health implications, close associations of macaques may cause a threat to human well-being.

Pathological conditions of the sentinel bivalve, the little mussel Brachidontes rodriguezii, from contaminated intertidal sites in the Southwestern Atlantic coast

A survey of pathological conditions affecting the natural beds of the dominant bivalve species of the intertidal rocky coasts of the Southern Atlantic Ocean, was performed. Samples of the little mussel (n = 992), Brachidontes rodriguezii, which is considered a sentinel species, were collected from seven sites that present different anthropogenic activity (low anthropogenic activity, harbor, and sewage waste) along the Argentinean coast, and processed by standard histological techniques. Our results showed for the first time in a B. rodriguezii population from Mar del Plata harbor a bacterium causing cellular lysis of the spermatogenic cells (6.5% of prevalence) and in a population from Villa Gesell, a site with low anthropogenic activity, one little mussel presented cytoplasmic vacuoles in the oocytes’s cytoplasm similar to a microsporidian (0.2% of prevalence). A ciliated protozoan parasitizing the cytoplasm of the epithelial digestive tubules and larval stages of two different digenean parasites, was found infecting the little mussel from both contaminated and uncontaminated sites. Sporocysts of the family Bucephalidae invaded the gonad, causing severe gonadal damage including castration, and gymnophallid metacercariae located between the mantle and shell or embedded in the mantle tissues, occupied the gonadal space.In Punta Carballido, a site located next to a sewage outfall, the highest prevalence of infection was found, for the ciliated protozoan in the digestive tubules (5.1%) and for the digenean parasites (bucephalid sporocyst 24.4%, and gymnophallid metacercariae 50%). Moreover, we found a positive correlation between shell length and overall prevalence of parasites.

Prevalence of cryptosporidiosis among children with diarrhoea under five years admitted to Kosti teaching hospital, Kosti City, Sudan

BackgroundCryptosporidiosis is a disease caused by infection with an intestinal coccidian parasite Cryptosporidium. Cryptosporidium species are the second leading cause of diarrheal disease and death in children in developing countries. Until now, no data have been available or published on its prevalence among children with diarrhea in Sudan. Therefore, this paper was designed to determine the prevalence rate of Cryptosporidium among children with diarrhea under 5 years who were admitted to Kosti Teaching Hospital.MethodsA hospital-based cross-sectional study including children under 5 years old admitted to the pediatric section of the hospital between September 2020 and December 2020. A total of one-hundred and fifty stool samples were collected. All stool samples were examined using the modified Ziehl Neelsen (mZN) staining technique and then examined microscopically for Cryptosporidium infection.ResultsA total of 150 children were examined out of which 70 presented with diarrhea. A greater prevalence of 19/70 (27.1%) of Cryptosporidium was observed in children with diarrhea than children without diarrhea 7/80 (8.8%). There was a significant relationship between the prevalence of Cryptosporidium and the presence of diarrhea in children under 5 years in the Kosti Teaching Hospital(P < 0.05). It was found that a higher prevalence was registered among children using piped-water sources for drinking.ConclusionsThe overall prevalence of parasite detected was 17.3% among children admitted to Kosti Teaching Hospital. The prevalence rate of the infection among Children with diarrhoea was 27.1%. Studying the prevalence rate of cryptosporidiosis among diarrheic children may predict their health status, leading to a better diagnosis, treatment, and, therefore, patients’ status improvement.

Bot Fly Parasitism of Allegheny Woodrats (Neotoma magister) in Virginia

The Allegheny woodrat (Neotoma magister) is a species of high conservation concern and relatively well-studied with respect to habitat use/associations, food habits, conservation genetics, and population trends. However, with the exception of raccoon roundworm (Baylisascaris procyonis) occurrence and etiology in woodrats, most disease and parasite ecology aspects for the woodrat are unknown. Herein, we examined the prevalence of bot flies (Cuterebra) over nearly three decades of woodrat surveys (1990–2018) in the central Appalachian Mountains of western Virginia. We use genetic analyses to identify recent bot fly specimen collections from a woodrat captured in 2017. Though highly variable from year to year, the overall prevalence of parasitism was low (typically < 4% of captures). As such, bot flies do not appear to be a widespread parasitic burden to Allegheny woodrats in Virginia. Genetic analysis of four collected bot fly larvae was inconclusive, as the genetic signature of these woodrat bots did not match any of the six bot species known to parasitize rodents and lagomorphs in the eastern United States. Further collections and genetic analyses will be needed to determine if the genetic database is incomplete or incorrect, or if our find is a new species of bot fly not yet taxonomically recognized.

Prevalence and risk factors of gastrointestinal nematode infestation of horses, donkeys and mules in tropical, dry and temperate regions in Mexico.

The aims of this study were to determine the prevalence of gastrointestinal nematodes (GIN) in horses, donkeys and mules and its associations with age, sex and climatic factors (derived from satellite data) to identify the potential risk factors of different climate regions in four Mexican states. From May 2017 to April 2018, a total of 560 farm owners or managers answered the questionnaire, and the data were used to establish three Köppen climate classes (tropical, dry, temperate). The overall prevalence of GIN parasites in equines was 77.9% (436 out of 560). The highest percentage of GIN was detected in mules (87.5%). In addition, the highest prevalence among the climate regions was found in the temperate climate (79.9%). The identified nematodes were strongyle type (77.9%), Parascaris equorum (5.7%), Strongyloides (0.7%) and Oxyuris equi (2.9%). The highest mean value of strongylid eggs per g faeces was found in the dry climate (632.6±96.8), followed by that in the tropical climate (518.3±49.7) and temperate climate (383.8±30.2); however, a similar prevalence was observed between different climate regions. Age and sex were identified as risk factors for high shedding of strongyle eggs: the odds ratios for higher shedding intensities were 3.858 for geldings compared to mares, 2.602 for 6-10-year-old equines and 3.597 for ≤16-year-olds compared to young equines (≤5years old).

Bot Fly Parasitism of Allegheny Woodrats (Neotoma magister) in Virginia

The Allegheny woodrat (Neotoma magister) is a species of high conservation concern and relatively well-studied with respect to habitat use/associations, food habits, conservation genetics, and population trends. However, with the exception of raccoon roundworm (Baylisascaris procyonis) occurrence and etiology in woodrats, most disease and parasite ecology aspects for the woodrat are unknown. Herein, we examined the prevalence of bot flies (Cuterebra) over nearly three decades of woodrat surveys (1990–2018) in the central Appalachian Mountains of western Virginia. We use genetic analyses to identify recent bot fly specimen collections from a woodrat captured in 2017. Though highly variable from year to year, the overall prevalence of parasitism was low (typically < 4% of captures). As such, bot flies do not appear to be a widespread parasitic burden to Allegheny woodrats in Virginia. Genetic analysis of four collected bot fly larvae was inconclusive, as the genetic signature of these woodrat bots did not match any of the six bot species known to parasitize rodents and lagomorphs in the eastern United States. Further collections and genetic analyses will be needed to determine if the genetic database is incomplete or incorrect, or if our find is a new species of bot fly not yet taxonomically recognized.

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The document associated with this DOI has been withdrawn.

Prevalence of gastrointestinal parasites in domestic pigeons and backyard chickens in Kalar and Khanaqin districts, Iraq

Domestic pigeons and backyard chickens are reared by a large number of people in Iraq for several purposes including meat, egg, commerce, and hobby. They are infected with different types of parasites which cause high mortality rate and great economic loss. The present study was conducted in two districts in Garmian region, Kalar and Khanaqin to study the prevalence of gastrointestinal parasites in domestic pigeons and backyard chickens. A total of 720 fresh fecal samples from (401 backyard chickens and 319 domestic pigeons) were examined using direct microscopy and standard floatation technique. The overall prevalence of parasites was 38.61 %. The rate of infection in chickens was 32.91% (132/401) and the rate of infection in pigeons was 45.76% (146/319). Five genera of nematodes were identified in chickens: Ascaridia galli (5.98%), Heterakis gallinarum (4.48%), Capillaria sp. (3.24%), Oxyspirura mansoni (1.74%) and Trichostrongylus tenuis (0.75%). Two cestodes were also identified, Choanotaenia infundibulatum (6.73%) and Raillietina sp. (0.75%) and one protozoan, Eimeria sp. (15.96%). Five genera of parasites were identified in domestic pigeons as follows: three nematodes, Ascaridia columbae (2.82%), Heterakis gallinarum (1.25%) and Capillaria sp. (0.62%), one cestode, Raillietina sp. (1.56%) and one protozoan, Eimeria sp. (42%). Sexes of the birds had no effect on the rate of infection p>0.05. Regarding locations, pigeons from Khanaqin were highly infected with parasites compared to Kalar p

Prevalence of dirofilariasis in shepherd and stray dogs in Iranshahr, southeast of Iran

Dirofilariasis is a zoonotic parasitic disease, which its cause Dirofilaria immitis, a nematode transmitted by insects and a worldwide dissemination. Dogs and cats are the main hosts of this parasite. Therefore, this study was conducted to determine the prevalence of D. immitis in shepherd and stray dogs in Iranshahr city, southeast of Iran. In this study 49 shepherd dogs and 50 stray dogs selected randomly and the blood samples were taken and sent to parasitology laboratory. Thin and thick thin blood samples were prepared and stained with Giemsa method and modified knott method. The obtained data were analyzed by SPSS 18 statistical software. The overall prevalence of parasite was 30.3%, seven of 49 shepherd dogs (14/3%) and twenty three of 50 stray dogs (46%) were positive. From 30 positive samples, 14.3% of shepherd dogs and 50% of stray dogs were male, and 44.7% of stray dogs were female. 14% of infected shepherd dogs and 38.5% of infected stray dogs were indigenous, and 16.7% of infected shepherd dogs and 72.7% of infected stray dogs were hybrids. There was no statistically significant relationship between the prevalence of D. immitis with age, gender, breed and usage of anti-parasitic drugs. Due to the relatively high contamination of dogs in the city of Iranshahr with this parasite, veterinarians and public health professionals should be considered necessary and preventive measures in relation to this disease.

Parasitism by Bat Flies on an Urban Population of Cynopterus brachyotis in Singapore

This study characterizes the patterns of bat fly parasitism on Cynopterus brachyotis, the most common fruit bat in the tropical urban environment of Singapore. Mist netting was conducted over a period of five months in two secondary forest sites, where 211 unique individuals were captured and examined for ectoparasites. The relative density, prevalence and mean intensity of parasitism by the wingless nycteriibid Leptocyclopodia ferrarii ferrarii were compared with respect to sex, age and reproductive status of captured bats. The overall prevalence of parasitism was 40.3% and analyses revealed no significant differences in the three parameters when comparisons were made between demographic subgroups. However, adults, females and pregnant females had a higher prevalence of infestation, while juveniles, males and non-pregnant females had higher mean intensity of bat flies. Roosting behavior is likely to be the driving factor for parasite transmission, as bat flies are more likely to encounter females because of C. brachyotis' harembased social structure. No correlation was found between bat body condition and parasite load, even when the relationship was examined for juveniles and adults separately. The observation of a re-infestation within 46 days suggests that there is high intraindividual and intra-roost parasite transmission. Research into roost group size, distribution, site fidelity, and connectivity is needed to better understand the patterns and dynamics of parasitism on bats living in the urban environment.

GASTROINTESTINAL PARASITES OF SHEPERD DOGS FROM TETOVO REGION MACEDONIA

A total of 78 faecal samples from owned shepherd dogs were collected in Tetovo, Macedonia and were examined for the presence of intestinal parasites by centrifugation - flotation method that is more sensitive. The overall prevalence of parasitism in the tested samples was 0,71%. The species found and the infective percentage were: Ancylostoma caninum 41%, Trichuris vulpis 39.2% and Toxocara canis 17,8. The parasite Angiostrongylus vasorum was found in only 1 sample. There was not observed any significant difference in prevalence between dogs genders according the species of parasite found. Young animals were found to more frequently shed nematode eggs in faeces than adult animals. The presence of zoonotic species of parasites in dogs in the studied region, associated with the elevated degree of misinformation of the owners, indicates that the risk of zoonotic infection by canine intestinal parasite may be high.

Variations in the Rate of Infestations of Dogs with Zoonotic Nematodes and the Contamination of Soil in Different Environments.

Companion animals are an important aspect in human life. However, they may also be considered a source of pathogens. An example of zoonotic parasitoses is toxocarosis or cutaneous larva migrans (CLM). The aim of the study was to detect zoonotic nematodes of dogs living in different areas and the intensity of contamination in parasite polluted environments that are hazardous to human health. The fecal samples were examined using standard flotation and decantation methods as well as McMaster’s quantitative technique. The soil samples in urban and rural areas were examined using a modified flotation method as described by Quinn et al. Statistical analyses were performed by IBM SPSS Statistics Version 23. The overall prevalence of parasites in dogs was 38%, 17.02% and 56.60% from urban and rural areas, respectively. The percentage values of nematodes important for human health (Toxocara canis, Ancylostomatidae, Trichuris vulpis) remained at the same level (16%). The infected dogs were dominated by a single parasite species, the main was T. canis (28.95%). In total, 54.30% of the soil samples were contaminated with parasite eggs. The contamination of urban and rural sandpits was 40% and 60%, respectively. The molecular examinations of soil samples using LAMP (loop-mediated isothermal amplification) confirmed the presence of nematode eggs of the species T. canis in all samples previously classified as positive

Elevada prevalencia de Blastocystis spp. en niños de una escuela periurbana

Introducción. La parasitosis intestinal es un grave problema de salud pública en países en vía de desarrollo; la blastocistosis es una parasitosis emergente. Objetivos. Determinar la prevalencia de infección por Blastocystis spp. y de otros enteroparásitos en niños de una escuela periurbana. Diseño. Estudio observacional y transversal. Lugar. Escuela ubicada en el distrito de Yura en Arequipa, Perú. Participantes. 83 niños, entre 4 y 8 años de edad. Intervenciones. Las muestras de heces de los niños fueron analizadas con el método Teleman modificado; se hizo un análisis descriptivo a través del cálculo de frecuencias. Principales medidas de resultados. Presencia del parásito intestinal. Resultados. La prevalencia de Blastocystis spp. fue 81,9% (68/83), la prevalencia global de parásitos fue 96,4% (80/83); además, 80,7% (67/83) tenía más de un tipo de parásito. Conclusiones. Existe una elevada prevalencia de Blastocystis spp. y de otros enteroparásitos; también, un elevado poliparasitismo en la población escolar estudiada. Es el primer estudio realizado en dicha comunidad.

THE PARASITIC PROFILE AMONG SCHOOL CHILDREN IN El-WADI EL-GADDED GOVERNORATE, EGYPT

Parasitic infection is still a serious public health problem in the world, especially in developing countries including Egypt. It represents a major cause of morbidity and mortality in childhood and among high-risk groups-in most parts of the world. This study detected the prevalence of parasitic infection among school children in-El-Wadi El-Gadded (the New Valley Governorate). A total of randomly chosen 1615 students aged from 6-16 years, (771 males & 844 female) from 12 primary schools and 12 preparatory schools related to four centers (El Dakhala, El Farfra, Paris and Platt) from the New Valley. Governorate. Each child was subject to: A questionnaire sheet, Urine examination through sedimentation methods, Stool examination using: Direct smear, Simple sedimentation method and Modified Ziehl-Neelsen Stain, Blood samples were collected randomly from 450 children and examined for Seropositivity of toxoplasmosis using (On-SiteToxolgG/IgM Rapid Test- Cassette) and examination of hair & clothes for ectoparasites (lice). The overall prevalence of parasites was (39.1%) among primary and preparatory school children. The helminthes were E. vermicularis (15.2% & 17.1%); A. lumbricoides (1.3% & 1.9%) and then H. nana (0.9% & 0.6%) and the protozoa were E. histolytica (14.1% & 13.2%), Giardia lamblia (3.8% & 3.9%), and then Cryptosporidium parvum (0.09%) and seropositivity of toxoplasmosis was in (3.0% & 2.7%) among primary and preparatory school children respectively. Mixed infection was in (0.4%) among primary school children. Head lice infestaiion was more prevalent among primary school children than preparatory school ones with a ratio (3.5% &0.2%) respectively, was nil among males.

Ticks infesting wild small rodents in three areas of the state of São Paulo, Brazil

ABSTRACT: From May to September 2011, a total of 138 wild rodents of the Cricetidae family were collected in the cities of Anhembi, Bofete and Torre de Pedra, in São Paulo State. All animals were examined for the presence of ticks, which were collected and identified at species level in the laboratory by morphological examination (for adults, nymphs and larvae) and molecular biology, by sequencing of a fragment of the 16S mitochondrial rDNA (for larvae). A total of 47 ticks (21 larvae and 26 nymphs) were collected from rodents, identified as 21 larvae and 23 nymphs of Amblyomma ovale (collected on Oligoryzomys nigripes , Oligoryzomys flavescens , Sooretamys agouya and Nectomys squamipes ), two nymphs of Ixodes schulzei (O. nigripes and O. flavescens ) and an Amblyomma sculptum nymph in S. agouya . The overall prevalence of parasitism by A. ovale was 4.3% for larvae, and 10.1% for nymphs. The mean intensity of parasitism was 3.5 larvae/parasitized animal, and 1.6 nymphs/parasitized animal. One O. nigripes was found parasitized by both larva and nymph of A. ovale , and another O. nigripes was parasitized simultaneously by an I. schulzei nymph and five A. ovale nymphs. This research reports the following unpublished records: A. ovale on O. flavescens , O. nigripes and S. agouya ; A. sculptum on S. agouya ; and I. schulzei on O. flavescens and O. nigripes .