Introduction: Secondary infertility indicates previous pregnancy but failure to conceive subsequently. Incidences of secondary infertility are increasing day by day. Many factors like age, hectic schedule, stress, diet, unhealthy lifestyle are responsible for it. Psychological stress disturbs overall health of women by inducing generation of reactive oxygen species and thereby oxidative stress. This oxidative stress may hamper the health of ovaries, oocyte quality and causes female reproductive health disorders. Aim: To study the effect of Ayurvedic treatment in management of unexplained secondary infertility. Objective: To assess the effect of Ayurvedic management of unexplained secondary infertility. Methodology: Single case study of unexplained secondary infertility. Case description: A 37 years old female patient came in ITRA OPD of Streeroga and Prasutitantra, trying to conceive since 1.5 years. She had a history of 2 MTPs and 1 female child of 10 year. Her last MTP was done in 2020. After that she was actively trying to conceive around last 1.5 years but couldn’t conceive again inspite of regular unprotected coitus. Treatment: She was treated with Ayurvedic oral medications along with Panchakarma. Result: After four months of treatment she conceived and followed Garbhini Paricharya. Conclusion: Ayurvedic management can act as an anti-stress and antioxidant agent thereby improving the reproductive health of women and increasing their chances of conception.