Coronavirus disease 2019 (COVID-19) is caused by a novel coronavirus named severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which may infect any cell type expressing ACE2 and TMPRSS2 receptors. ACE2 is expressed in several human ovarian compartments, and relatively highly expressed in oocytes. Therefore, the ovary and oocyte might be potential targets of SARS-CoV-2. Information concerning the susceptibility of the female reproductive systems to SARS-CoV-2 infection, and possible effects on embryo development remain inconsistent. Time-lapse imaging (TLI) systems allow for the mapping of morphological changes or events with the exact time-point of occurrence. The aim of this study was to investigate the impact of SARS-CoV-2 infection on embryo morphokinetic development. This historical cohort study enrolled 884 injected oocytes from 88 female patients undergoing ICSI from Mar/2019 to Jun/2021. Female patients were age matched in a 1:3 ratio, to either a COVID group, including patients with a positive SARS-CoV-2 immunoglobulin test (n=22 patients, 152 injected oocytes), or a control group, including patients with a negative SARS-COV-2 immunoglobulin test (n=66, 732 injected oocytes). A sample of at least 111 embryos had 95% power to detect a 20% effect with alpha of 5%. Embryos were cultured in the EmbryoScope incubator, which recorded the following kinetic markers: timing to pronuclei appearance and fading (tPNa and tPNf), two (t2), three (t3), four (t4), five (t5), six (t6), seven (t7), and eight cells (t8), morulae (tM), start of blastulation (tSB) and blastulation (tB). Durations of second and third cell cycles (cc2 and cc3) and timing to complete synchronous divisions s1, s2, and s3 were calculated. The KIDScore ranking was recorded. The impact of SARS-COV-2 on embryo morphokinetic events and ICSI outcomes were investigated considering clustering of data. Embryos derived from patients in the COVID group presented longer tPNa (7.4 ± 0.26 vs. 6.71 ± 0.18, p< 0.001), tPNf (24.503 ± 0.47 vs. 23.386 ± 0.336, p=0.003), t2 (26.980 ± 0.489 vs. 26.001 ± 0.344, p=0.032), t3 (37.992 ± 0.659 vs. 36.146 ± 0.461, p=0.005), t4 (41.027 ± 0.677 vs. 37.745 ± 0.471, p< 0.001), t5 (51.856 ± 1.067 vs. 48.636 ± 0.722, p< 0.001) and tB (109.122 ± 0.804 vs. 106.244 ± 1.429, p=0.028). Durations of cc3 (14.187 ± 0.65 vs. 12.806 ± 0.25, p=0.049), s1 (2.397 ± 0.895 vs. 2.732 ± 0.306, p< 0.001), s2 (3.073 ± 0.36 vs. 1.871 ± 0.146, p=0.002) were also significantly increased in the COVID group compared with the control group, while KIDScore D5 ranked significantly lower in the COVID group (5.3 ± 0.3 vs. 6.3 ± 0.1, P=0.005). Previous SARS-CoV-2 maternal infection had significant impacts on embryo morphokinetic events and KIDScore rank.