BackgroundThe COVID-19 pandemic has highlighted the need for effective infection control in outpatient healthcare settings. Germicidal Ultraviolet-C (GUV) light, known for inactivating microorganisms by damaging their DNA or RNA, offers a potential solution. This study examines the efficacy of GUV air disinfection systems in real-world outpatient environments. MethodsWe deployed upper-room and far-UV GUV fixtures in three outpatient facilities, assessing their impact on bacterial loads through air and surface sampling and bioindicator tests. Occupancy was also monitored. ResultsWhile manual air and surface sampling did not show a significant difference in bacterial loads between control and UV-C-treated groups, bioindicator tests demonstrated a high level of spore inactivation (up to 99.7% for upper-room GUV and 96.26% for far-UV). Occupancy levels did not significantly influence these outcomes. DiscussionThe discrepancy between bioindicator efficacy and environmental sampling results suggests limitations in the latter's ability to accurately capture environmental bioburden. Bioindicators proved to be reliable for in-situ validation of UV-C surface disinfection. ConclusionsBioindicators are effective for validating GUV surface disinfection efficacy in healthcare settings, though further research is needed to optimize environmental sampling methods for assessing GUV's impact on real-world bacterial loads.