The taxonomic review of the ostracods from Solimões Formation in the western Amazonas State, Brazil, allowed the identification of eight genera and twenty-two species of the genus Cyprideis Jones, 1857. Two species are new: Cyprideis goeldiensis sp. nov and Cyprideis javariensis sp. nov.; and Cyprideis sp. 1 remains in open nomenclature. For the first time, the species Cyprideis santaelenae Sousa and Ramos 2023, originally described to the Pebas Formation, in Peru, was recorded in the Solimões Formation. A new morphotype of the species Cyprideis matorae Gross et al. 2014 is here identified. The stratigraphical distribution of the Cyprideis species in the borehole 1AS-32-AM permitted the identification of four ostracod zones: Cyprideis caraionae Zone, Cyprideis minipunctata Zone, Cyprideis cyrtoma Zone and Cyprideis paralela Zone, presenting a time range from the middle Miocene (Serravallian) to the late Miocene (Tortonian). Based on the ostracod assemblage, an environment of a semiconfined lake, with mesohaline features and sporadic marine influence is attested to the western Amazonia during the Miocene. The species turnover of Cyprideis occurred during the Tortonian.