Osteoprotegerin In Patients Research Articles

Prognostic effect of osteoprotegerin in patients with ischemic stroke: A systematic review and meta-analysis.

Osteoprotegerin (OPG) is supposed to participate in the development of atherosclerosis and cardio-cerebrovascular disease. However, the results of research on relationship between OPG and ischemic stroke (IS) are controversial. Therefore, we carried out the first systematic review and meta-analysis to evaluate prognostic effect of osteoprotegerin in patients with IS. We comprehensively searched databases of PubMed, Embase, and the Cochrane Library through 21 August 2023 to identify observational studies that evaluated effect of OPG on poor functional outcome (modified Rankin Scale [mRS] Score of 3-6) and mortality in patients with IS. Adjusted odds ratios (aOR) with a 95% confidence interval (CI) of each included study were used as much as possible to assess the pooled effect. Five studies that enrolled 4,506 patients in total fulfilled our inclusion criteria. Three studies were included in the pooled analysis for each endpoint since one of the included studies had provided data on poor functional outcome as well as mortality. OPG was neither associated with poor functional outcome (aOR 1.29, 95% CI 0.90-1.85) nor with mortality (aOR 1.57, 95% CI 0.90-2.74) in patients with IS. There is insufficient evidence to demonstrate the correlation between OPG and mortality or poor functional outcome in IS patients. OPG cannot be applied to predict worse neurological function in IS patients based on the current evidence.

Parathyroid Hormone on Osteoprotegerin Levels in Patients with Primary Hyperparathyroidism.

Osteoprotegerin is a glycoprotein that plays a major role in the regulation of bone turnover. The influence of parathyroid hormone, an important regulator of bone remodeling, on osteoprotegerin production is controversial. The purpose of the study was to assess the influence of parathyroid hormone on the circulating level of osteoprotegerin in patients with primary hyperparathyroidism by comparing it with healthy controls. Forty-four patients with biochemical verification of primary hyperparathyroidism scheduled for the surgical cure and 38 healthy subjects were included. Blood samples of the study group were taken before surgery. Levels of serum parathyroid hormone, osteoprotegerin, calcium, 25-hydroxyvitamin D [25(OH)D], and alkaline phosphatase were analyzed. Bone mineral density at the L1-L4 vertebrae and femoral neck was calculated by dual-energy X-ray absorptiometry. Osteoprotegerin levels and bone mineral density values were significantly lower in patients than in the healthy subjects (P=.002 and P > .0001, respectively). There was no correlation between osteoprotegerin and parathyroid hormone in the groups. Osteoprotegerin was weakly correlated with bone mineral density in patients. No correlation was noted between osteoprotegerin and bone mineral density in the control group. Furthermore, osteoprotegerin levels were not correlated with calcium, 25(OH)D, and alkaline phosphatase levels in each group. The production of osteoprotegerin appears to be inhibited by parathyroid hormone in patients with primary hyperparathyroidism. A weak positive correlation found among osteoprotegerin and bone mineral density recommends that osteoprotegerin may be a molecule that impacts bone metabolism and finally bone mineral density.

Механізми ремоделювання кісткової тканини у осіб молодого віку з остеоартритом, що протікає на фоні ожиріння

The purpose of the study was to determine the content of osteoprotegerin in the blood serum of patients with osteoarthritis, based on the stage of obesity and the state of bone mineral density. Materials and methods. Under surveillance there were 75 patients with osteoarthritis, which proceeded against the background of an altered body mass index. The comparison group consisted of 50 patients with osteoarthritis and normal body weight. The age of patients in both groups was comparable, and gender balance was observed between groups. The osteoprotegerin index (pg/ml) was studied in fasting blood serum by enzyme immunoassay (ELISA) using of the FineTest EH0247 reagents, China. The prevalence of osteoporotic conditions was assessed using dual-energy x-ray absorptiometry (DEXA), HOLOGIC Explorer QDR W Series Bone Densitometer (USA). Statistical analysis was performed using the software package Statistics 10.0 and Excel 2010. Cardinal and ordinal changes were compared using the Mann-Whitney test. Correlation was calculated using Spearman's rank correlations. In all statistical analysis procedures, the level of significance p was taken equal to or less than 0.05 (p <0.05). Based on body mass index, all patients in the main group were divided into 3 subgroups. Thus, changes in body weight were recorded in 75 patients with osteoarthritis (main group). 1st stage of obesity was found in 31 patients (42%); 2nd – 22 patients (29%) and 22 patients (29%) with osteoarthritis were overweight. Results and discussion. When studying the content of osteoprotegerin in the examined patients, it was found that both in the isolated course of osteoarthritis (92.29±1.68 pg/ml) and in its combination with obesity (124.03±3.2 pg/ml), there was an increase in this indicator when compared with the results of patients in the control group (65.64±0.64, p <0.05). In order to study the state of bone mineral density in patients with osteoarthritis, a densitometric study was performed using double energy X-ray absorptiometry – DEHA (device "Expert 1188", Lunar, USA), which allowed to diagnose osteoporotic conditions. Thus, signs of osteopenia were found in 11 patients (15%) of the main and 8 people (16%) of the comparison group. Manifestations of osteoporosis were found in 18 (24%) and 5 people (10%), respectively. In patients of the main group with osteopenia, the content of osteoprotegerin in the blood serum corresponded to 108.43±0.48 pg/ml, in the comparison group – 84.86±0.87 pg/ml. With the development of osteoporosis, this value exceeded the control values, however, it was somewhat lower than with osteopenia: 99.94±1.1 and 73.57±1.66 pg/ml, respectively. Conclusion. The course of osteoarthritis is accompanied by an increase in the production of osteoprotegerin, which indicates a violation of bone tissue remodeling through increased processes of its resorption. The level of blood serum osteoprotegerin in patients with osteoarthritis has a significant increase with weight gain and its maximum value is observed in patients with 2nd stage of obesity. In patients with osteoarthritis, the formation of osteoporotic conditions (osteopenia or osteoporosis) is observed, one of the markers of which can be considered an increase in the content of osteoprotogerin glycoprotein – cytokine, which is characterized by inhibition of osteoclast production

Pathogenetic and diagnostic role of osteoprotegerin in the combined course of osteoarthritis and obesity

In recent years, it has been proven that the formation of OA can occur against the background of impaired bone metabolism.
 Bone remodeling is considered as a continuous complex process aimed at eliminating microdamages and updating the bone matrix. A key link in the regulation of this process is the system RANK / RANKL / OPG (osteoprotegerin), which provides a balance of activity of osteoblasts and osteoclasts. It blocks the interaction between RANK and RANKL by intercepting the RANKL ligand, which inhibits osteoclast development and reduces bone resorption.
 Thus, these properties of OPG can be considered as one of the pathogenetic factors in patients with dystrophic joint lesions.
 Objective: to investigate the content and role of osteoprotegerin in young patients with osteoarthritis, which occurs against the background of changes in body weight.
 Materials and methods. The study involved 75 young patients (mean age - 30.92 ± 0.546 years) with overweight or obesity, who in the previous stages of the study was diagnosed with osteoarthritis (main group). The comparison group was represented by 50 patients with osteoarthritis and normal body weight. The age of patients was 30.95 ± 0.545 years; as in the previous group, men predominated - 64% and 36% respectively.
 Benchmarks of osteoprotegerin were obtained in a study of 37 relatively healthy individuals of the same age and sex.
 The level of osteoprotegerin was determined by enzyme-linked immunosorbent assay using the FineTest EH0247 reagent kit, China.
 The outcomes were processed by the methods of variation statistics using the computer program STATISTICA.Results and discussion. When determining the content of OPG in the serum of patients of the main group was found to increase to 124.03 pg / ml, against control - 65.64 pg / ml. In the group of patients with isolated OA, this value was 92.29 pg / ml. Meaning that, in OA, running on the background of altered BMI, the content of OPG is likely to increase, both in relation to the norm and the results of the comparison group (p <0,001).
 The formation and course of osteoarthritis is accompanied by an increase in osteoprotegerin, the content of which depends on the degree of obesity and the radiological stage of the process.
 Conclusions: The course of osteoarthritis is accompanied by an increase in osteoprotegerin, which is considered a negative regulator of bone resorption.
 In osteoarthritis, which occurs against the background of changes in body mass index, a direct correlation with the degree of obesity.
 Changes in osteoprotegerin in patients with osteoarthritis depend on the radiological stage of joint damage and are most pronounced in individuals with stage 2 obesity and stage 2 radiological changes

Serum Levels of Osteoprotegerin in Patients with Acne Vulgaris

Serum Levels of Osteoprotegerin in Patients with Acne Vulgaris

Effect of Omega 3 Fatty Acids on the Vascular Calcification Biomarkers Fetuin A and Osteoprotegerin in Patients With CRF

Effect of Omega 3 Fatty Acids on the Vascular Calcification Biomarkers Fetuin A and Osteoprotegerin in Patients With CRF

Prognostic significance of arterial stiffness and osteoprotegerin in patients with stable coronary artery disease.

Arterial stiffness and vascular calcification significantly contribute to coronary atherosclerosis progression. The prognostic value of increased arterial stiffness and vascular calcification in subjects with stable coronary artery disease (CAD) after percutaneous coronary intervention(PCI) is currently under question. We randomly enrolled 262 patients with stable CAD 1month after successful PCI. Carotid-femoral pulse wave velocity (PWV) was measured as a well-established index of central aortic stiffness. Osteoprotegerin (OPG) plasma levels were measured as a biomarker of vascular calcification. Patients were followed up prospectively up to 52months. The primary endpoint was the composite of death from cardiovascular causes, myocardial infarction, stroke or hospitalization for cardiovascular causes. During the follow-up period, 48 patients presented the primary composite endpoint. Subjects who presented the primary endpoint, compared to subjects free of cardiovascular events, had significantly increased PWV (9.45±2.19m/s vs 8.73±2.07m/s, P=.04) and OPG levels (4.21±2.19pmol/L vs 3.18±1.74pmol/L, P=.003). Survival analysis indicated that PWV predicted adverse cardiac events MACE (Hazard ratio=1.29 95%CI: 1.07-1.57, P=.008) independently from confounders such as age, sex, smoking habits, ejection fraction, extent of coronary artery disease, hypertension and diabetes mellitus. Interestingly, for every increase in pulse wave velocity by 1m/s, there is an anticipated increase in the risk of major adverse cardiovascular event (MACE) by 29%. These findings extend the current knowledge concerning the role of arterial stiffness as powerful biomarkers in cardiovascular disease. Measurement of PWV might have a role in ascertaining prognosis and managing treatment in patients with stable CAD after PCI.

Serum Levels of Fibroblast Growth Factor-23, Osteoprotegerin, and Receptor Activator of Nuclear Factor Kappa B Ligand in Patients with Prolactinoma

The aim of this study to was to evaluate the effect of fibroblast growth factor-23 (FGF-23), osteoprotegerin (OPG), receptor activator nuclear κB ligand (RANKL), and vitamin D hormones on bone loss in patients with hyperprolactinemia due to pituitary prolactinoma. We recruited 46 premenopausal female patients with prolactinoma and age and sex-matched healthy controls (Group 3, n = 20) for this cross-sectional study. Prolactinoma patients were divided into 2 groups as patients newly diagnosed (Group 1, n = 26) and those under cabergoline treatment (Group 2, n = 20). Anthropometric and metabolic variables; hormonal profiles; and osteocalcin, deoxypyridinoline (DOP), and bone mineral density measurements were performed for all participants. FGF-23, OPG, and RANKL levels were analyzed in all groups. FGF-23, OPG, calcium, phosphorus, and parathormone levels were similar between all groups despite significantly higher levels in the control group in terms of vitamin D and RANKL levels than in patients. Bone loss was found more in Group 2, particularly observed in Z scores of femur and spinal bone (P<.05). Correlation analysis revealed a negative correlation between FGF-23 and femur neck T score (r = -0.0433, P = .05) in patients with active prolactinoma. A positive correlation was also observed between parameters of DOP and OPG (r = 0.673, P = .02). In patients with remission there were a negative correlation between prolactin and luteinizing hormone (r = -600, P = .08). Additionally, a negative correlation was found between osteocalcin and osteoprotegerin in patients in remission (r = -0.73, P = .01). Our data indicated that FGF-23 and OPG levels do not play a critical role on the development of bone decrease in patients with hyperprolactinemia. However, further prospective studies in larger numbers of participants should be designed to clarify this issue. BFP = body fat percentage BMD = bone mineral density BMI = body mass index CV = coefficient of variation DOP = deoxypyridinoline ELISA = enzyme-linked immunosorbent assay FGF-23 = fibroblast growth factor-23 HOMA-IR = homeostatic model assessment of insulin resistance OPG = osteoprotegerin RANKL = receptor activator nuclear κB ligand.

Serum Osteoprotegerin in Patients with Calcified Aortic Valve Stenosis in Relation to Heart Failure.

The aim of the study was to assess the role of serum osteoprotegerin (OPG) as a biomarker in patients with aortic valve stenosis (AS) in relation to heart failure and symptomatic status. This was a case control study, which included 51 patients with AS and 39 control subjects. At the time of study enrolment, detailed medical history was obtained and all subjects underwent physical examination, chest x-ray and echocardiography. OPG levels were measured in all subjects, and serum N-terminal of the pro b-type natriuretic peptide (NT pro BNP) levels were determined in patients with AS. Serum OPG levels were elevated in patients with AS compared to control subjects (p=0.001). Patients with heart failure due to AS had elevated serum OPG levels in comparison to patients without heart failure (p=0.001). A significant correlation between OPG and symptomatic status was observed in all patients with AS (p<0.001), however, it was not the case in patients without heart failure (p=0.425). There was a positive correlation between OPG and NT pro BNP concentrations with objective signs of heart failure on chest x-ray (p<0.001). Negative correlation of OPG concentrations with aortic valve area was present (p<0.040), as well as with left ventricular ejection fraction (p<0.001). Serum OPG could be a valuable biomarker in the evaluation of severity of calcified AS and serve as an additional indicator besides clinical presentation and echocardiography in the assessment of surgical treatment or aortic valve replacement.

Diagnostic and Prognostic Properties of Osteoprotegerin in Patients with Acute Dyspnoea: Observations from the Akershus Cardiac Examination (ACE) 2 Study.

BackgroundCirculating osteoprotegerin (OPG) levels are increased in patients with chronic heart failure (HF). The diagnostic and prognostic merit of OPG measurement in patients admitted with acute dyspnoea is unknown.ObjectivesTo evaluate the diagnostic and prognostic value of measuring OPG in patients admitted to hospital with acute dyspnoea.MethodsOPG was analysed by ELISA in 308 patients admitted due to acute dyspnoea. Investigators blinded to OPG results adjudicated the diagnosis for the index hospitalization. Clinical outcomes were obtained from hospital records.ResultsIn total, 139 patients (45%) were hospitalized with acute HF. OPG levels on hospital admission were higher in patients with acute HF vs. no acute HF, 7.8 (5.5–10.4) vs. 5.4 (3.8–7.2) pmol/L, p<0.001. The area under the receiver operator characteristic curve (ROC AUC) of OPG to discriminate between HF vs. non-HF was 0.695 [95% CI 0.636–0.754]. OPG did not provide incremental information to the ED physician’s prediction or N-terminal pro-B-type natriuretic peptide regarding the diagnosis of acute HF. OPG levels (log transformed) were associated with mortality in crude analysis (HR (95% CI) 1.87 (1.34 to 2.61), p<0.001), but this association was attenuated and no longer significant after including established cardiac biomarkers into the model.ConclusionIn patients admitted to hospital with acute dyspnoea, OPG levels are higher in patients with acute HF than in those with dyspnoea from other causes. However, OPG does not provide incremental information beyond ED physician assessment for the diagnosis of acute HF or beyond clinical risk variables and established cardiac biomarkers concerning prognosis.

Effects of Minocycline on Urine Albumin, Interleukin-6, and Osteoprotegerin in Patients with Diabetic Nephropathy: A Randomized Controlled Pilot Trial.

BackgroundWe tested minocycline as an anti-proteinuric adjunct to renin-angiotensin-aldosterone system inhibitors (RAASi) in diabetic nephropathy (DN) and measured urinary biomarkers to evaluate minocycline’s biological effects.MethodsDesign: Prospective, single center, randomized, placebo-controlled, intention-to-treat pilot trial. Inclusion. Type 2 diabetes/DN; Baseline creatinine clearance > 30 mL/min; proteinuria ≥ 1.0 g/day; Age ≥30 years; BP <150/95 mm Hg; intolerant of/at maximum RAASi dose. Protocol. 3-wk screening; Baseline randomization; Urine and blood measures at months 1, 2, 4, and Month 6 study completion. Urine interleukin-6 (IL-6) and osteoprotegerin were measured in a subset. Primary outcome. Natural log of urine protein/creatinine (ln U P:Cr) ratio at Month 6 vs Baseline.Results30 patients completed the study. The 15% decline in U P: Cr in minocycline patients (6 month P:Cr ÷ Baseline P:Cr, 0.85 vs. 0.92) was not significant (p = 0.27). Creatinine clearance did not differ in the 2 groups. Urine IL-6:Cr (p = 0.03) and osteoprotegerin/Cr (p = 0.046) decrements were significant. Minocycline modified the relationship between urine IL-6 and proteinuria, suggesting a protective biological effect.ConclusionsAlthough the decline in U P:Cr in minocycline patients was not statistically significant, the significant differences in urine IL-6 and osteoprotegerin suggest that minocycline may confer cytoprotection in patients with DN, providing a rationale for further study.Trial RegistrationClinicaltrials.gov NCT01779089

Effect of metformin treatment on circulating osteoprotegerin in patients with nonalcoholic fatty liver disease.

Growing evidence suggests complex interplay between nonalcoholic fatty liver disease (NAFLD) and bone health. The present study's aim was to examine the impact of metformin treatment on circulating osteoprotegerin (OPG) in patients with NAFLD, a population in which this relationship has not yet been studied. In a randomized, placebo-controlled study, 63 patients with NAFLD were assigned to one of two groups: Group 1 received daily metformin; Group 2 received a placebo. Metabolic parameters, insulin resistance markers and OPG levels were examined at baseline and at the end of the study. In the placebo group, liver function and OPG levels did not change during the study. Among metformin-treated patients, significant declines in OPG and alkaline phosphatase were observed. CRP and ALT decreased marginally during the 4-month treatment period. While at baseline circulating OPG levels did not differ significantly between the groups, by the end of the study OPG was significantly lower in patients treated with metformin than in the placebo group (p<0.0001). Delta OPG was significantly greater in the metformin group than the placebo group (p=0.001). In the general linear model, metformin treatment was the only significant independent predictor of endpoint and delta OPG. Metformin treatment was associated with a significant decrease in OPG levels in patients with NAFLD. The effect on OPG was associated with exposure to metformin per se. NCT01084486.

Osteoprotegerin in patients with degenerative aortic stenosis and preserved left-ventricular ejection fraction

ObjectivesThe aim of the present study was to evaluate value of osteoprotegerin (OPG) in patients with degenerative aortic stenosis and preserved left-ventricular ejection fraction.MethodsWe have prospectively followed 70 patients with aortic stenosis (mean aortic gradient ≥15 mmHg) and preserved le

Effects of Combination Therapy With Olmesartan and Azelnidipine on Serum Osteoprotegerin in Patients With Hypertension

Vascular calcification is a potent predictor of plaque instability and cardiac events. Osteoprotegerin (OPG), well-known vascular calcification mediator, is a signaling molecule involved in bone remodeling, which has been implicated in the regulation of vascular calcification and atherogenesis. The purpose of this study was to compare the combination treatments of olmesartan/azelnidipine and olmesartan/diuretics on serum bone-related markers in patients with essential hypertension. A total of 48 patients with hypertension treated with 20 mg olmesartan were randomized to receive combination treatment with 16 mg azelnidipine (O/A group) or diuretics (1 mg indapamide; O/D group) for 12 months. Osteoprotegerin, matrix metalloproteinase 2 (MMP-2), and high-sensitive CRP (hs-CRP) were measured after 3 and 12 months of treatment. Cardio-ankle vascular index (CAVI) was measured as the arterial stiffness using a VaSera CAVI instrument at the same time points. In both groups, the systolic and diastolic blood pressure reduction is similar. Serum OPG, MMP-2, and hs-CRP were significantly decreased at 12 months in the O/A group (P < .05), while there were no significant reductions in the O/D group. CAVI was significantly improved at 12 months in both the treatment groups. The improvement in CAVI was significantly greater in the O/A group than in the O/D group. Azelnidipine, but not indapamide, combined with olmesartan, improved arterial stiffness and were associated with significant decrease in OPG, MMP-2, and hs-CRP concentrations. These results suggest that the beneficial effects of the combination treatments of olmesartan/azelnidipine on arterial stiffness are mediated by alteration in bone-remodeling and inflammatory markers.

Detection of autoantibodies to osteoprotegerin in patients with rheumatoid arthritis and their association with disease activity

Searchable abstracts of presentations at key conferences on calcified tissues ISSN 2052-1219 (online)

Relationship between Bone Mineral Density and Serum Osteoprotegerin in Patients with Chronic Heart Failure

PurposeHeart failure (HF) had been reported with increased risk of hip fractures. However, the relationship between circulating biomarkers and bone mineral density (BMD) in chronic HF remained unclear.MethodsThis is a cross-sectional study which recruited stable chronic HF from registry of the Heart Failure Center of National Taiwan University Hospital. Patients underwent dual-energy x-ray absorptiometry (DEXA) measurements at hip and lumbar spines and biochemical assessments including B-type natriuretic peptide (BNP-32), myostatin, follistatin and osteoprotegerin (OPG).ResultsA total of 115 stable chronic HF individuals with left ventricular ejection fraction (EF) <45% (74% of male, mean age at 59) were recruited with 24 patients in NYHA class I, 73 patients in NYHA class II and 18 patients in NYHA class III. Results of BMD showed that Z scores of hip in NYHA III group (−0.12±1.15) was significantly lower than who were NYHA II (0.58±1.04). Serum OPG was significantly higher in subjects of NYHA III (9.3±4.6 pmol/l) than NYHA II (7.4±2.8 pmol/l) or NYHA I (6.8±3.6 pmol/l) groups. There’s a significant negative association between log transformed serum OPG and trochanteric BMD (R = −0.299, P = 0.001), which remained significant after multivariate analysis.ConclusionsOur study demonstrated an inverse association between serum OPG and trochanteric BMD in patients with HF. OPG may be a predictor of BMD and an alternative to DEXA for identifying at risk HF patients for osteoporosis.

Detection of autoantibodies to osteoprotegerin in patients with severe idiopathic osteoporosis

Detection of autoantibodies to osteoprotegerin in patients with severe idiopathic osteoporosis

Enzyme replacement therapy (ENB-0040) in hypophosphatasia improves functional outcome and decreases TNSALP substrates in adolescents and adults

Hypophosphatasia (HPP), a heritable metabolic bone disease, results from inactivating mutation(s) in the gene for the tissue non-specific isoenzyme of alkaline phosphatase (TNSALP). The natural substrates that accumulate in HPP include inorganic pyrophosphate (PPi), an inhibitor of mineralization, and pyridoxal 5′ phosphate (PLP), the principal circulating form of vitamin B6. PPi excess blocks hydroxyapatite crystal formation causing rickets in growing children and osteomalacia in adults. There is no approved therapy. ENB-0040, a bone-targeted, recombinant, human TNSALP given subcutaneously has improved severely affected infants and children with HPP. Objective: Evaluate TNSALP substrate levels following 24 weeks of ENB-0040 treatment (Rx) and six-minute walk test (6MWT) function in adolescents and adults with HPP. The 6MWT is a performance-based measure of functional exercise capacity. Methods: 6 adolescents and 13 adults with HPP (mean age 42 yrs [14–68]) were randomized in an open-label, multicenter, multinational, dose-ranging, concurrent control study of the safety, efficacy, and pharmacokinetics of ENB-0040. Patients received 0, 2.1, or 3.5 mg/kg/wk of ENB-0040. Results: At baseline (BL), patients averaged 349 m during the 6MWT (6–620 m), but 10 required assistive devices. The differences walked for the Rx vs no Rx groups were +26 vs −14 m at week 24. Among those with functional impairment (n=12) whose BL 6MWT was 25%N75% of normal, 9 improved (8/9 Rx, and 1/3 no Rx). The mean improvements for the 2.1 and 3.5 mg/kg/wk groups were +35.4 m (n=5) and +43.5 m (n=4), respectively. Injection site reactions occurred in 7 patients, but did not cause discontinuation of treatment. Six serious adverse events (SAEs) were reported among 3 patients, two observation patients had 4 of the SAEs. No SAEs were from ENB-0040. Serum PLP levels decreased in both groups, from 324 and 602 ng/mL at BL to 69 ng/mL and 38 ng/L at week 24, respectively (p=0.009). Conclusion: ENB-0040 significantly decreases TNSALP substrates and improves the 6MWT in adolescents and adults with HPP. This article is part of a Special Issue entitled ECTS 2012. Disclosure of interest: M. Whyte grant/research support from Enobia, consulting fees from Enobia, C. Greenberg grant/research support from Enobia, Advisory Board Membership of Enobia, consulting fees from Enobia, P. Kishnani grant/research support from Enobia, Advisory Board Membership of Enobia, consulting fees from Enobia, K. Madson grant/research support from Enobia, A. Mhanni grant/research support from Enobia, T. Weber: none declared, K. Mack grant/research support from Enobia, A. Reeves grant/research support from Enobia, H. Plotkin employee of Enobia, N. Kreher employee of Enobia, H. Landy employee of Enobia. doi:10.1016/j.bone.2012.02.101 OC31 (recipient of a 2012 ECTS Travel Award) Detection of autoantibodies to osteoprotegerin in patients with severe idiopathic osteoporosis P. Riches⁎, T. Gilchrist, W.D. Fraser, S.H. Ralston Rheumatology Department, Institute of Genetics and Molecular Medicine, Edinburgh, UK Faculty of Medicine and Health Sciences, University of East Anglia,

No evidence of circulating autoantibodies against osteoprotegerin in patients with celiac disease

To investigate risk factors for low bone mineral density (BMD) in celiac disease (CD) patients, focusing on circulating autoantibodies against osteoprotegerin (OPG). Seventy asymptomatic CD adult patients on gluten-free diet (GFD) and harbouring persistent negative CD-related serology were recruited. Conventional risk factors for osteoporosis (e.g., age, sex, menopausal status, history of fractures, smoke, and body mass index) were checked and BMD was assessed by dual energy X ray absorptiometry. Serum calcium and parathyroid hormone (PTH) levels were evaluated. Thirty-eight patients underwent repeat duodenal biopsy. Serum samples from a selected sub-group of 30 patients, who were also typed for human leukocyte antigen (HLA) DQ2 and DQ8 haplotype, were incubated with homodimeric recombinant human OPG and tested by western blotting with an anti-OPG antibody after immunoprecipitation. Despite persistent negative CD-related serology and strict adherence to GFD, 49 out of the 70 (74%) patients displayed low BMD. Among these patients, 13 (24%) showed osteoporosis and 36 (76%) osteopenia. With the exception of age, conventional risk factors for osteoporosis did not differ between patients with normal and low BMD. Circulating serum calcium and PTH levels were normal in all patients. Duodenal mucosa healing was found in 31 (82%) out of 38 patients who underwent repeat duodenal biopsy with 20 (64%) still displaying low BMD. The remaining 7 patients had an incomplete normalization of duodenal mucosa with 6 (84%) showing low BMD. No evidence of circulating antibodies against OPG was found in the serum of 30 celiac patients who were tested for, independent of BMD, duodenal histology, and HLA status. If any, the role of circulating autoantibodies against OPG in the pathogenesis of bone derangement in patients with CD is not a major one.

Evaluation of serum matrix Gla protein with osteoprotegerin in patients with rheumatoid arthritis

Evaluation of serum matrix Gla protein with osteoprotegerin in patients with rheumatoid arthritis