This study outlines a theoretical model that governs how the transmission of mechano-stimulation affects the proliferation of osteoblast-like (MC3T3-E1) bone cells attached to a deformable silasticnano-bioactive membrane. The nano-bioactive silastic membrane was functionalized by exposure to ultraviolet oven (UV Ozone) treatment regimes, coated with an extra-cellular matrix (ECM) protein, which is fibronectin (FN) and then seeded with MC3T3-E1 cells. The proliferation of MC3T3-E1 cells were evaluated after the cells were plated on the functionalized FN-coated nano-bioactive membrane surfaces and subjected to a 2 hour mechano-stimulation comprising of the application of a 2% dynamic equi-biaxial strain at 1 Hz frequency cycle. Elevation of the phalloidin-stained cellular actin cytoskeleton was observed, indicating the occurrence of mechanical transduction of the applied strains through the cell integrin receptors which culminated in the reorganization of the cellular cytoskeleton. The classical theory of elastic membrane deformation, therefore, rightly predicts mechano-transduction between the bioengineered nano-bioactive membrane surfaces and the applied mechanical force strains, via the integrin receptors, to the MC3T3-E1 cells, leading to enhanced cellular proliferation.