Osteoarthritis In Life Research Articles

EFFICACY OF ERANDAMOOLADI BASTI IN THE MANAGEMENT OF JANU SANDHIGATAVATA VIS-A-VIS OSTEOARTHRITIS OF KNEE JOINT – AN OBSERVATIONAL CLINICAL STUDY

Janu Sandhigata vata is one such Vatavyadhi presenting with Sandhi shula, Sandhi shotha, Sandhi atopa and Prasarana Akunchana Vedana, resulting in limited range of movements, there by restricting daily activities of the individual. Janu Sandhigatavata is commonly identified with Osteoarthritis of Knee Joint characterized by pain, swelling & impaired movements. The prevalence of Osteoarthritis rises progressively with age and it has been estimated that that 45% of all people develop Knee Osteoarthritis in life. Osteoarthritis is more common in women than men. The existent line of treatment in contemporary field of medicine includes the administration of analgesics, NSAIDS, Intra articular steroids and surgical intervention in the form of Knee replacement, which all has their own limitation and meanwhile may have deleterious adverse effect. In this regard, there is a need for exploring the safe, effective and affordable line of treatment. Basti karma is a supreme treatment for Vata Vyadhi and also known as Ardha Chikitsa because of its multifaceted actions. Many Basti formulations are mentioned in classics among which Erandamooladi Bati was selected for the study because its dual action, does Vataghna and also relives Kapha Avarana. The results were statistically highly significant within the group.

Blocking mechanosensitive ion channels eliminates the effects of applied mechanical loading on chick joint morphogenesis.

Abnormalities in joint shape are increasingly considered a critical risk factor for developing osteoarthritis in life. It has been shown that mechanical forces during prenatal development, particularly those due to fetal movements, play a fundamental role in joint morphogenesis. However, how mechanical stimuli are sensed or transduced in developing joint tissues is unclear. Stretch-activated and voltage-gated calcium ion channels have been shown to be involved in the mechanoregulation of chondrocytes in vitro. In this study, we analyse, for the first time, how blocking these ion channels influences the effects of mechanical loading on chick joint morphogenesis. Using in vitro culture of embryonic chick hindlimb explants in a mechanostimulation bioreactor, we block stretch-activated and voltage-gated ion channels using, respectively, gadolinium chloride and nifedipine. We find that the administration of high doses of either drug largely removed the effects of mechanical stimulation on growth and shape development in vitro, while neither drug had any effect in static cultures. This study demonstrates that, during joint morphogenesis, mechanical cues are transduced—at least in part—through mechanosensitive calcium ion channels, advancing our understanding of cartilage development and mechanotransduction.This article is part of the Theo Murphy meeting issue ‘Mechanics of development’.