OSOM Trichomonas Rapid Test Research Articles

Prevalence and Risk Factors for Trichomonas Vaginalis Infection Among Women: a Population-Based Controlled Study in Saudi Arabia.

Information on Trichomonas vaginalis (T. vaginalis) infection in Saudi Arabia is scarce. The aim of study was to assess the burden and risk factors of T. vaginalis infection for a cohort of women living in Saudi Arabia. Women aged ≥ 18 years who were seeking medical care at the King Faisal Medical Complex Gynecology Clinic in Taif city, Western Saudi Arabia, were enrolled in a non-randomized case-control study between June 2018 and May 2019. Participants were interviewed using a standard questionnaire for a number of sociodemographic and clinical characteristics. Vaginal swabs obtained from each participant were screened for T. vaginalis infection with direct wet mount smear microscopy, the OSOM Trichomonas rapid test 'OSOM Trich' (Genzyme Diagnostics, Cambridge, MA, USA) and a published nested PCR. Over the study period, 155 women were recruited: 79 with symptoms of vaginitis (i.e. cases) and 76 with no symptoms (i.e. controls). The T. vaginalis infection was detected in ~20% (16/79) of cases and ~9% (7/76) of the controls by the nested PCR. Using the PCR test results as a gold standard, the wet mount microscopy's sensitivity, specificity, negative predictive value, and positive predictive value were 69.5%, 100%, 94.9%, and 100%, respectively, whereas the OSOM Trich's were 86.9%, 100%, 97.7%, and 100%, respectively. The main high-risk factors included age between 30 and 39 years (~35%), marriage for 10 - 30 years (~62%), non-education (~41%), urban residence (~29%), and employment (~36%). Highly significant differences were observed concerning infection distribution among cases for the presence of lower abdominal pain (~64%) and abnormal vaginal discharge (38%) as presenting symptoms (χ2 = 20.42; p < 0.001 and χ2 = 5.63; p = 0.017, respectively). The burden of infection with T. vaginalis is unexpectedly high in the population studied. Regular screening for T. vaginalis infection, particularly in high-risk women, is required.

Comparative Analysis of Various Diagnostic Methods for Trichomonas Vaginalis Infection in Trinidad and Tobago

Trichomonas vaginalis infection is a common sexually transmitted infection in the reproductive age group, which may lead to complications. Wet mount examination is the most common test for diagnosis, but it has low sensitivity. Fluorescent antibody staining can be used for diagnosis, but it is expensive and requires special microscopic facility. Culture is considered as the gold standard, but it takes a long time for diagnosis and not practical for routine use. OSOM Trichomonas Rapid Test is a recently introduced rapid method based on immunochromatographic assay of trichomonal protein antigens. Nucleic Acid Amplification Tests (NAAT’s) are highly sensitive, it requires expensive resources and is currently not widely available in developing countries. The present study was done to compare these four available diagnostic techniques for detection of trichomoniasis. Samples were collected from a total of six hundred ninety two (692) patients. Wet mount examination, Fluorescent antibody staining, InPouch culture and OSOM Trichomonas Rapid Test were performed. Out of 692 patients included in our study, Four hundred and twenty two (422) were females and Two hundred seventy (270) were males. 12.4% positive cases of Trichomoniasis were detected by InPouch culture. OSOM Trichomonas Rapid Test detected in (11.2%) cases and Fluorescent antibody staining detected in 9.3% cases, whereas, wet mount examination detected only 3.5% positive cases. OSOM Trichomonas Rapid Test showed sensitivity and specificity of 90.5% and 98.5%, respectively. Comparative analysis of four diagnostic methods indicates OSOM Trichomonas Rapid Test is a easily performed simple test with higher sensitivity and specificity than both wet mount examination and Fluorescent antibody staining. Standardized strips of high quality with reasonable costing could be recommended as a routine test in clinic as well as in rural settings.

Trichomonasspp. in Pigeons: Detection by OSOM Trichomonas Rapid Test

The efficacy of the OSOM Trichomonas Rapid Test (developed for rapid diagnosis of human Trichomonas vaginalis) in detection of Trichomonas spp. in pigeons (Columba livia) was investigated. Two oral cavity swabs were taken from 50 farm pigeons. Cultivation in Diamond Trichomonas medium was used as a reference method. According to a morphological determination, Trichomonas gallinae was the only protozoan found; however, no further molecular analysis was conducted. The OSOM Trichomonas test was positive in 39 oral swabs. In comparison with the cultivation method three samples were identified as false negative and one as false positive. Test specificity and sensitivity were established as 93% and 90%, respectively. Using Cohen's Kappa, the concordance between the two testing methods was found to be strong (kappa = 0.7506, 95% CI = 0.5162-0.9850). The OSOM Trichomonas test is not able to distinguish between Trichomonas species; however, results suggest that the test is suitable for the rapid detection of Trichomonas spp. infection in pigeons.

O06.1 Evaluation of 5 Different Tests For Trichomonas Vaginalis (TV) Infection and Cost Effective Planning For Clinical Implementation

BackgroundTV is the most common non-viral STI in the world. Despite this, TV infection in UK Genitourinary clinics is mainly (and often exclusively) diagnosed by wet mount microscopy alone. Microscopy...

Performance of the OSOM Trichomonas Rapid Test for diagnosis of Trichomonas vaginalis infection among women in Mysore, India

Background Trichomonas vaginalis is the world's most common treatable sexually transmissible infection. Currently, wet mount microscopy and syndromic management based on vaginal discharge are the most widely used methods for diagnosing and treating trichomoniasis in resource-constrained settings. Wet mount microscopy requires equipment and trained technicians, who are in short supply. We examined the diagnostic accuracy of the OSOM Trichomonas Rapid Test for detecting T. vaginalis vaginal infection among women in Mysore, India. During July 2009-August 2010, 450 sexually active women over 18 years seeking care at an urban reproductive health clinic were enrolled in the study. Clinician-collected vaginal swabs were evaluated for trichomonads using wet mount microscopy, InPouch culture and the OSOM test. Of the 418 samples included in the analyses, culture detected 68 (16.3%) positive samples, wet mount microscopy detected 56 of the culture-positive samples and four false positive samples. The OSOM test detected 60 of the culture-positive samples plus two false positive cases. Using the composite reference standard (CRS), defined as wet mount- or culture-positive, the sensitivities of wet mount, the OSOM test and culture were 83.3%, 86.1% and 94.4%, respectively. The positive and negative predictive values of the OSOM test were 100% and 97.1% respectively. The Cohen's kappa agreement between the OSOM test and the CRS was excellent (κ=0.94). The OSOM test has high sensitivity, excellent specificity, and excellent positive and negative predictive value compared to a CRS. This simple test can improve screening and diagnosis of T. vaginalis infection in resource-constrained settings where microscopy and culture are unavailable.

P3-S7.01 Evaluation of a simple point-of-care rapid test for detecting Trichomonas vaginalis among women in Mysore, India

BackgroundTrichomonas vaginalis is one of the easily treatable sexually transmitted infections in the world. Current methods used to diagnose trichomoniasis rely heavily on training and experience of the technician and...

Use of an Immunochromatographic Assay for Rapid Detection of Trichomonas vaginalis in Vaginal Specimens

Trichomonas vaginalis infection is estimated to be the most widely prevalent nonviral sexually transmitted infection in the world. Wet-mount microscopy is the most common diagnostic method, although it is less sensitive than culture. The OSOM Trichomonas Rapid Test (Genzyme Diagnostics, Cambridge, Mass.) (referred to here as OSOM) is a new point-of-care diagnostic assay for T. vaginalis that uses an immunochromatographic capillary flow (dipstick) assay and provides results in 10 min. The purpose of this study was to determine the test characteristics of OSOM compared to those of a composite reference standard (CRS) comprised of wet-mount microscopy and T. vaginalis culture. This multicenter cross-sectional study enrolled sexually active women > or =18 years of age who presented with symptoms of vaginitis, exposure to T. vaginalis, or multiple sexual partners. Vaginal-swab specimens were obtained for T. vaginalis culture, wet mount, and rapid testing. The prevalence of T. vaginalis in this sample was 23.4% (105 of 449) by the CRS. The sensitivity and specificity of OSOM vaginal-swab specimens were 83.3 and 98.8%, respectively, while wet mount had a sensitivity and specificity of 71.4 and 100%, respectively, compared to the CRS. OSOM performed significantly better than wet mount (P = 0.004) and detected T. vaginalis in samples that required 48 to 72 h of incubation prior to becoming culture positive. The performance of the rapid test was not affected by the presence of coinfections with chlamydia and gonorrhea. The OSOM Trichomonas Rapid Test is a simple, objective test that can be expected to improve the diagnosis of T. vaginalis, especially where microscopy and culture are unavailable.